Restriction map for
pF1KA0523
(Linear) (Six-base) MAPSORT of: KIAA0523.seq Check: 9320 from: 1 to: 1725
KIAA0523.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AccI GT'mk_AC
Cuts at: 0 551 989 1725
Size: 551 438 736
Fragments arranged by size:
736 551 438
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 1725
Size: 429 1296
AflIII A'CryG_T
Cuts at: 0 1091 1526 1725
Size: 1091 435 199
Fragments arranged by size:
1091 435 199
AleI CACnn'nnGTG
Cuts at: 0 1235 1725
Size: 1235 490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 112 229 1507 1725
Size: 112 117 1278 218
Fragments arranged by size:
1278 218 117 112
AlwNI CAG_nnn'CTG
Cuts at: 0 51 875 969 1725
Size: 51 824 94 756
Fragments arranged by size:
824 756 94 51
ApaI G_GGCC'C
Cuts at: 0 136 394 1725
Size: 136 258 1331
Fragments arranged by size:
1331 258 136
ApaLI G'TGCA_C
Cuts at: 0 1462 1725
Size: 1462 263
ApoI r'AATT_y
Cuts at: 0 1312 1725
Size: 1312 413
AvaI C'yCGr_G
Cuts at: 0 137 224 394 828 1725
Size: 137 87 170 434 897
Fragments arranged by size:
897 434 170 137 87
BanI G'GyrC_C
Cuts at: 0 141 427 1103 1334 1491 1725
Size: 141 286 676 231 157 234
Fragments arranged by size:
676 286 234 231 157 141
BanII G_rGCy'C
Cuts at: 0 136 213 271 394 658 718 1619 1725
Size: 136 77 58 123 264 60 901 106
Fragments arranged by size:
901 264 136 123 106 77 60 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 205 239 994 1028 1725
Size: 205 34 755 34 697
Fragments arranged by size:
755 697 205 34 34
BclI T'GATC_A
Cuts at: 0 1643 1725
Size: 1643 82
BglI GCCn_nnn'nGGC
Cuts at: 0 178 1725
Size: 178 1547
BlpI GC'TnA_GC
Cuts at: 0 536 1725
Size: 536 1189
Bme1580I G_kGCm'C
Cuts at: 0 136 144 394 1101 1337 1466 1496 1721
Size: 136 8 250 707 236 129 30 225
Cuts at: 1721 1725
Size: 4
Fragments arranged by size:
707 250 236 225 136 129 30 8
4
BmgBI CAC'GTC
Cuts at: 0 1422 1725
Size: 1422 303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 672 1725
Size: 672 1053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 702 1725
Size: 7 695 1023
Fragments arranged by size:
1023 695 7
Bpu10I CC'TnA_GC
Cuts at: 0 1613 1725
Size: 1613 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 1420 1725
Size: 516 904 305
Fragments arranged by size:
904 516 305
BsaAI yAC'GTr
Cuts at: 0 1094 1725
Size: 1094 631
BsaWI w'CCGG_w
Cuts at: 0 305 1283 1658 1725
Size: 305 978 375 67
Fragments arranged by size:
978 375 305 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 667 697 1725
Size: 667 30 1028
Fragments arranged by size:
1028 667 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1486 1555 1725
Size: 1486 69 170
Fragments arranged by size:
1486 170 69
BseYI C'CCAG_C
Cuts at: 0 183 246 787 875 1725
Size: 183 63 541 88 850
Fragments arranged by size:
850 541 183 88 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1725
Size: 199 1526
BsiEI CG_ry'CG
Cuts at: 0 1596 1725
Size: 1596 129
BsiHKAI G_wGCw'C
Cuts at: 0 1466 1725
Size: 1466 259
BsmI GAATG_Cn'
Cuts at: 0 886 1725
Size: 886 839
Bsp1286I G_dGCh'C
Cuts at: 0 136 144 213 271 394 658 718 1101
Size: 136 8 69 58 123 264 60 383
Cuts at: 1101 1337 1466 1496 1619 1721 1725
Size: 236 129 30 123 102 4
Fragments arranged by size:
383 264 236 136 129 123 123 102
69 60 58 30 8 4
BspEI T'CCGG_A
Cuts at: 0 305 1283 1658 1725
Size: 305 978 375 67
Fragments arranged by size:
978 375 305 67
BspHI T'CATG_A
Cuts at: 0 359 1725
Size: 359 1366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 923 1725
Size: 923 802
BsrBI CCG'CTC
Cuts at: 0 540 1545 1725
Size: 540 1005 180
Fragments arranged by size:
1005 540 180
BsrFI r'CCGG_y
Cuts at: 0 85 556 593 677 1595 1725
Size: 85 471 37 84 918 130
Fragments arranged by size:
918 471 130 85 84 37
BsrGI T'GTAC_A
Cuts at: 0 1018 1725
Size: 1018 707
BssSI C'ACGA_G
Cuts at: 0 460 1231 1404 1725
Size: 460 771 173 321
Fragments arranged by size:
771 460 321 173
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 1725
Size: 162 1563
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1237 1725
Size: 1237 488
Bsu36I CC'TnA_GG
Cuts at: 0 956 1725
Size: 956 769
BtgI C'CryG_G
Cuts at: 0 176 744 815 926 1725
Size: 176 568 71 111 799
Fragments arranged by size:
799 568 176 111 71
BtsI GCAGTG_nn'
Cuts at: 0 516 1725
Size: 516 1209
DrdI GACnn_nn'nnGTC
Cuts at: 0 226 1725
Size: 226 1499
EaeI y'GGCC_r
Cuts at: 0 2 170 456 860 945 1378 1593 1725
Size: 2 168 286 404 85 433 215 132
Fragments arranged by size:
433 404 286 215 168 132 85 2
EagI C'GGCC_G
Cuts at: 0 1593 1725
Size: 1593 132
EarI CTCTTCn'nnn_
Cuts at: 0 617 1360 1725
Size: 617 743 365
Fragments arranged by size:
743 617 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 589 744 1215 1725
Size: 25 564 155 471 510
Fragments arranged by size:
564 510 471 155 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 429 702 1725
Size: 7 422 273 1023
Fragments arranged by size:
1023 422 273 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 1725
Size: 799 926
EcoO109I rG'GnC_Cy
Cuts at: 0 132 133 391 951 1298 1725
Size: 132 1 258 560 347 427
Fragments arranged by size:
560 427 347 258 132 1
EcoRI G'AATT_C
Cuts at: 0 1312 1725
Size: 1312 413
HaeII r_GCGC'y
Cuts at: 0 184 1602 1725
Size: 184 1418 123
Fragments arranged by size:
1418 184 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 699 1199 1231 1725
Size: 667 32 500 32 494
Fragments arranged by size:
667 500 494 32 32
HincII GTy'rAC
Cuts at: 0 1392 1725
Size: 1392 333
HindIII A'AGCT_T
Cuts at: 0 1071 1725
Size: 1071 654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 780 1725
Size: 42 738 945
Fragments arranged by size:
945 738 42
MscI TGG'CCA
Cuts at: 0 4 458 862 947 1725
Size: 4 454 404 85 778
Fragments arranged by size:
778 454 404 85 4
MslI CAynn'nnrTG
Cuts at: 0 1235 1725
Size: 1235 490
MspA1I CmG'CkG
Cuts at: 0 108 299 344 604 746 1351 1725
Size: 108 191 45 260 142 605 374
Fragments arranged by size:
605 374 260 191 142 108 45
NaeI GCC'GGC
Cuts at: 0 558 1597 1725
Size: 558 1039 128
Fragments arranged by size:
1039 558 128
NcoI C'CATG_G
Cuts at: 0 926 1725
Size: 926 799
NgoMIV G'CCGG_C
Cuts at: 0 556 1595 1725
Size: 556 1039 130
Fragments arranged by size:
1039 556 130
NspI r_CATG'y
Cuts at: 0 261 781 1120 1725
Size: 261 520 339 605
Fragments arranged by size:
605 520 339 261
PasI CC'CwG_GG
Cuts at: 0 372 387 1706 1725
Size: 372 15 1319 19
Fragments arranged by size:
1319 372 19 15
PfoI T'CCnGG_A
Cuts at: 0 718 1424 1725
Size: 718 706 301
Fragments arranged by size:
718 706 301
PmlI CAC'GTG
Cuts at: 0 1094 1725
Size: 1094 631
PpuMI rG'GwC_Cy
Cuts at: 0 951 1298 1725
Size: 951 347 427
Fragments arranged by size:
951 427 347
PspOMI G'GGCC_C
Cuts at: 0 132 390 1725
Size: 132 258 1335
Fragments arranged by size:
1335 258 132
PspXI vC'TCGA_Gb
Cuts at: 0 224 1725
Size: 224 1501
PstI C_TGCA'G
Cuts at: 0 107 158 266 710 1725
Size: 107 51 108 444 1015
Fragments arranged by size:
1015 444 108 107 51
PvuII CAG'CTG
Cuts at: 0 1351 1725
Size: 1351 374
RsrII CG'GwC_CG
Cuts at: 0 731 1725
Size: 731 994
SacII CC_GC'GG
Cuts at: 0 747 1725
Size: 747 978
SapI GCTCTTCn'nnn_
Cuts at: 0 1360 1725
Size: 1360 365
SfcI C'TryA_G
Cuts at: 0 103 154 262 706 1725
Size: 103 51 108 444 1019
Fragments arranged by size:
1019 444 108 103 51
SmaI CCC'GGG
Cuts at: 0 139 1725
Size: 139 1586
SmlI C'TyrA_G
Cuts at: 0 224 495 1435 1725
Size: 224 271 940 290
Fragments arranged by size:
940 290 271 224
SphI G_CATG'C
Cuts at: 0 1120 1725
Size: 1120 605
StyI C'CwwG_G
Cuts at: 0 857 926 1725
Size: 857 69 799
Fragments arranged by size:
857 799 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 1725
Size: 238 1487
TatI w'GTAC_w
Cuts at: 0 1018 1725
Size: 1018 707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 767 799 1725
Size: 767 32 926
Fragments arranged by size:
926 767 32
Tth111I GACn'n_nGTC
Cuts at: 0 1387 1725
Size: 1387 338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 813 1056 1725
Size: 813 243 669
Fragments arranged by size:
813 669 243
XhoI C'TCGA_G
Cuts at: 0 224 1725
Size: 224 1501
XmaI C'CCGG_G
Cuts at: 0 137 1725
Size: 137 1588
XmnI GAAnn'nnTTC
Cuts at: 0 850 1725
Size: 850 875
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BcgI BclI BglI BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstXI Bsu36I BtgI BtsI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII
HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII RsrII SacII SapI SfcI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BciVI BfrBI BglII BmrI BmtI BsaI
BsaBI BsaHI BsiWI BsmBI BsrDI BssHII BstBI BstEII
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EcoICRI
EcoRV FalI FseI FspI FspAI HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PvuI SacI SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI XbaI ZraI