Restriction map for
pF1KSDA0526
(Linear) (Six-base) MAPSORT of: KIAA0526.seq Check: 9536 from: 1 to: 1686
KIAA0526.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 484 1686
Size: 484 1202
Acc65I G'GTAC_C
Cuts at: 0 1632 1686
Size: 1632 54
AclI AA'CG_TT
Cuts at: 0 1126 1686
Size: 1126 560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1480 1630 1686
Size: 1004 476 150 56
Fragments arranged by size:
1004 476 150 56
AfeI AGC'GCT
Cuts at: 0 99 1686
Size: 99 1587
AhdI GACnn_n'nnGTC
Cuts at: 0 333 1686
Size: 333 1353
AleI CACnn'nnGTG
Cuts at: 0 223 1686
Size: 223 1463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 956 988 1686
Size: 956 32 698
Fragments arranged by size:
956 698 32
ApaI G_GGCC'C
Cuts at: 0 1060 1686
Size: 1060 626
ApoI r'AATT_y
Cuts at: 0 723 926 1686
Size: 723 203 760
Fragments arranged by size:
760 723 203
AvaI C'yCGr_G
Cuts at: 0 1099 1686
Size: 1099 587
BamHI G'GATC_C
Cuts at: 0 1095 1686
Size: 1095 591
BanI G'GyrC_C
Cuts at: 0 1048 1291 1438 1632 1686
Size: 1048 243 147 194 54
Fragments arranged by size:
1048 243 194 147 54
BanII G_rGCy'C
Cuts at: 0 14 1060 1686
Size: 14 1046 626
Fragments arranged by size:
1046 626 14
BbeI G_GCGC'C
Cuts at: 0 1052 1442 1686
Size: 1052 390 244
Fragments arranged by size:
1052 390 244
BbsI GAAGACnn'nnnn_
Cuts at: 0 970 1397 1686
Size: 970 427 289
Fragments arranged by size:
970 427 289
BfrBI ATG'CAT
Cuts at: 0 790 1686
Size: 790 896
BglI GCCn_nnn'nGGC
Cuts at: 0 1064 1686
Size: 1064 622
Bme1580I G_kGCm'C
Cuts at: 0 1060 1686
Size: 1060 626
BmgBI CAC'GTC
Cuts at: 0 1226 1686
Size: 1226 460
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 473 1686
Size: 473 1213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 623 1172 1382 1686
Size: 446 177 549 210 304
Fragments arranged by size:
549 446 304 210 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 607 982 1686
Size: 291 316 375 704
Fragments arranged by size:
704 375 316 291
BsaAI yAC'GTr
Cuts at: 0 87 221 1686
Size: 87 134 1465
Fragments arranged by size:
1465 134 87
BsaHI Gr'CG_yC
Cuts at: 0 1049 1353 1439 1686
Size: 1049 304 86 247
Fragments arranged by size:
1049 304 247 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 419 439 449 469 590 620 1686
Size: 419 20 10 20 121 30 1066
Fragments arranged by size:
1066 419 121 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 105 604 673 1686
Size: 105 499 69 1013
Fragments arranged by size:
1013 499 105 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 1232 1686
Size: 630 602 454
Fragments arranged by size:
630 602 454
BsiEI CG_ry'CG
Cuts at: 0 7 1686
Size: 7 1679
BsiHKAI G_wGCw'C
Cuts at: 0 218 1686
Size: 218 1468
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1344 1654 1686
Size: 1344 310 32
Fragments arranged by size:
1344 310 32
Bsp1286I G_dGCh'C
Cuts at: 0 14 218 1060 1686
Size: 14 204 842 626
Fragments arranged by size:
842 626 204 14
BspHI T'CATG_A
Cuts at: 0 459 1686
Size: 459 1227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1199 1686
Size: 1199 487
BsrFI r'CCGG_y
Cuts at: 0 124 1686
Size: 124 1562
BsrGI T'GTAC_A
Cuts at: 0 377 1314 1686
Size: 377 937 372
Fragments arranged by size:
937 377 372
BstAPI GCAn_nnn'nTGC
Cuts at: 0 758 1219 1686
Size: 758 461 467
Fragments arranged by size:
758 467 461
BstYI r'GATC_y
Cuts at: 0 131 964 1095 1686
Size: 131 833 131 591
Fragments arranged by size:
833 591 131 131
BstZ17I GTA'TAC
Cuts at: 0 485 1686
Size: 485 1201
BtsI GCAGTG_nn'
Cuts at: 0 1220 1686
Size: 1220 466
DraI TTT'AAA
Cuts at: 0 1574 1686
Size: 1574 112
EaeI y'GGCC_r
Cuts at: 0 4 126 406 1686
Size: 4 122 280 1280
Fragments arranged by size:
1280 280 122 4
EagI C'GGCC_G
Cuts at: 0 4 1686
Size: 4 1682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 623 1004 1172 1382 1480 1630 1686
Size: 446 177 381 168 210 98 150 56
Fragments arranged by size:
446 381 210 177 168 150 98 56
EcoO109I rG'GnC_Cy
Cuts at: 0 914 1057 1596 1649 1686
Size: 914 143 539 53 37
Fragments arranged by size:
914 539 143 53 37
EcoRI G'AATT_C
Cuts at: 0 723 1686
Size: 723 963
EcoRV GAT'ATC
Cuts at: 0 256 1686
Size: 256 1430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1035 1576 1686
Size: 1035 541 110
Fragments arranged by size:
1035 541 110
HaeII r_GCGC'y
Cuts at: 0 101 1052 1442 1686
Size: 101 951 390 244
Fragments arranged by size:
951 390 244 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 439 471 1686
Size: 319 32 88 32 1215
Fragments arranged by size:
1215 319 88 32 32
HindIII A'AGCT_T
Cuts at: 0 182 1686
Size: 182 1504
KasI G'GCGC_C
Cuts at: 0 1048 1438 1686
Size: 1048 390 248
Fragments arranged by size:
1048 390 248
KpnI G_GTAC'C
Cuts at: 0 1636 1686
Size: 1636 50
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1467 1686
Size: 1467 219
MslI CAynn'nnrTG
Cuts at: 0 223 937 1264 1686
Size: 223 714 327 422
Fragments arranged by size:
714 422 327 223
MspA1I CmG'CkG
Cuts at: 0 1608 1686
Size: 1608 78
NaeI GCC'GGC
Cuts at: 0 126 1686
Size: 126 1560
NarI GG'CG_CC
Cuts at: 0 1049 1439 1686
Size: 1049 390 247
Fragments arranged by size:
1049 390 247
NgoMIV G'CCGG_C
Cuts at: 0 124 1686
Size: 124 1562
NsiI A_TGCA'T
Cuts at: 0 792 1686
Size: 792 894
NspI r_CATG'y
Cuts at: 0 1425 1686
Size: 1425 261
PasI CC'CwG_GG
Cuts at: 0 1053 1686
Size: 1053 633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 1220 1686
Size: 1188 32 466
Fragments arranged by size:
1188 466 32
PpuMI rG'GwC_Cy
Cuts at: 0 1596 1686
Size: 1596 90
PsiI TTA'TAA
Cuts at: 0 467 511 1686
Size: 467 44 1175
Fragments arranged by size:
1175 467 44
PspOMI G'GGCC_C
Cuts at: 0 1056 1686
Size: 1056 630
PstI C_TGCA'G
Cuts at: 0 105 1686
Size: 105 1581
PvuII CAG'CTG
Cuts at: 0 1608 1686
Size: 1608 78
ScaI AGT'ACT
Cuts at: 0 615 1084 1686
Size: 615 469 602
Fragments arranged by size:
615 602 469
SexAI A'CCwGG_T
Cuts at: 0 1336 1686
Size: 1336 350
SfcI C'TryA_G
Cuts at: 0 101 414 1241 1686
Size: 101 313 827 445
Fragments arranged by size:
827 445 313 101
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1064 1686
Size: 1064 622
SfoI GGC'GCC
Cuts at: 0 1050 1440 1686
Size: 1050 390 246
Fragments arranged by size:
1050 390 246
SmlI C'TyrA_G
Cuts at: 0 270 586 997 1686
Size: 270 316 411 689
Fragments arranged by size:
689 411 316 270
StyI C'CwwG_G
Cuts at: 0 1299 1686
Size: 1299 387
TatI w'GTAC_w
Cuts at: 0 377 613 1082 1314 1686
Size: 377 236 469 232 372
Fragments arranged by size:
469 377 372 236 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 419 451 590 622 1458 1490 1686
Size: 419 32 139 32 836 32 196
Fragments arranged by size:
836 419 196 139 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1200 1686
Size: 1200 486
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AhdI AleI AloI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BfrBI BglI Bme1580I BmgBI BplI BpmI BpuEI BsaAI
BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrFI BsrGI BstAPI BstYI BstZ17I BtsI
DraI EaeI EagI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HindIII KasI KpnI MmeI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PasI PpiI PpuMI
PsiI PspOMI PstI PvuII ScaI SexAI SfcI SfiI
SfoI SmlI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AflII AflIII AgeI AlfI AlwNI ApaLI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BclI BglII BlpI BmrI BmtI Bpu10I BsaI BsaBI
BsaWI BseYI BsiWI BsmI BspEI BsrBI BsrDI BssHII
BssSI BstBI BstEII BstXI Bsu36I BtgI BtgZI ClaI
CspCI DraIII DrdI EarI EciI EcoICRI EcoNI FseI
FspI FspAI HincII HpaI MfeI MluI MscI NcoI
NdeI NheI NotI NruI PacI PciI PflMI PfoI
PmeI PmlI PshAI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI