Restriction map for
pF1KA0530
(Linear) (Six-base) MAPSORT of: KIAA0530.seq Check: 6313 from: 1 to: 6792
KIAA0530.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2747 6792
Size: 2747 4045
AatII G_ACGT'C
Cuts at: 0 3283 6792
Size: 3283 3509
AccI GT'mk_AC
Cuts at: 0 71 879 5387 6792
Size: 71 808 4508 1405
Fragments arranged by size:
4508 1405 808 71
AclI AA'CG_TT
Cuts at: 0 6571 6792
Size: 6571 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1208 1226 2595 3373 3821 3884 3995
Size: 1166 42 18 1369 778 448 63 111
Cuts at: 3995 4127 4571 4796 5234 5246 5789 6430 6586
Size: 132 444 225 438 12 543 641 156
Cuts at: 6586 6661 6792
Size: 75 131
Fragments arranged by size:
1369 1166 778 641 543 448 444 438
225 156 132 131 111 75 63 42
18 12
AfeI AGC'GCT
Cuts at: 0 760 6792
Size: 760 6032
AflII C'TTAA_G
Cuts at: 0 1816 6792
Size: 1816 4976
AflIII A'CryG_T
Cuts at: 0 4480 5978 5980 6792
Size: 4480 1498 2 812
Fragments arranged by size:
4480 1498 812 2
AgeI A'CCGG_T
Cuts at: 0 2100 6792
Size: 2100 4692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5966 6792
Size: 5966 826
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3454 3486 6792
Size: 3454 32 3306
Fragments arranged by size:
3454 3306 32
AlwNI CAG_nnn'CTG
Cuts at: 0 652 4282 6289 6792
Size: 652 3630 2007 503
Fragments arranged by size:
3630 2007 652 503
ApaI G_GGCC'C
Cuts at: 0 2674 6792
Size: 2674 4118
ApoI r'AATT_y
Cuts at: 0 271 323 661 711 986 1629 1641 1762
Size: 271 52 338 50 275 643 12 121
Cuts at: 1762 1912 2319 2743 2919 3414 3735 3822 3843
Size: 150 407 424 176 495 321 87 21
Cuts at: 3843 3921 4139 4397 4450 4503 5010 5134 5165
Size: 78 218 258 53 53 507 124 31
Cuts at: 5165 6498 6792
Size: 1333 294
Fragments arranged by size:
1333 643 507 495 424 407 338 321
294 275 271 258 218 176 150 124
121 87 78 53 53 52 50 31
21 12
AseI AT'TA_AT
Cuts at: 0 6097 6792
Size: 6097 695
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5969 6002 6792
Size: 5969 33 790
Fragments arranged by size:
5969 790 33
BamHI G'GATC_C
Cuts at: 0 6487 6792
Size: 6487 305
BanI G'GyrC_C
Cuts at: 0 3136 6792
Size: 3136 3656
BanII G_rGCy'C
Cuts at: 0 1352 2674 6376 6792
Size: 1352 1322 3702 416
Fragments arranged by size:
3702 1352 1322 416
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 496 755 1987 3361 3872 6792
Size: 17 479 259 1232 1374 511 2920
Fragments arranged by size:
2920 1374 1232 511 479 259 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5414 5448 6792
Size: 5414 34 1344
Fragments arranged by size:
5414 1344 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5789 6792
Size: 5789 1003
BclI T'GATC_A
Cuts at: 0 837 1014 1110 6792
Size: 837 177 96 5682
Fragments arranged by size:
5682 837 177 96
BfrBI ATG'CAT
Cuts at: 0 3181 6792
Size: 3181 3611
BglII A'GATC_T
Cuts at: 0 1228 2355 5046 6792
Size: 1228 1127 2691 1746
Fragments arranged by size:
2691 1746 1228 1127
Bme1580I G_kGCm'C
Cuts at: 0 2674 2801 4521 6792
Size: 2674 127 1720 2271
Fragments arranged by size:
2674 2271 1720 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 2163 2800 3116 5769 6792
Size: 2163 637 316 2653 1023
Fragments arranged by size:
2653 2163 1023 637 316
BmtI G_CTAG'C
Cuts at: 0 4651 6792
Size: 4651 2141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 842 6792
Size: 842 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1187 1886 4172 4397 6390 6792
Size: 1187 699 2286 225 1993 402
Fragments arranged by size:
2286 1993 1187 699 402 225
Bpu10I CC'TnA_GC
Cuts at: 0 1351 2696 6792
Size: 1351 1345 4096
Fragments arranged by size:
4096 1351 1345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2834 3772 3955 4948 6439 6792
Size: 2834 938 183 993 1491 353
Fragments arranged by size:
2834 1491 993 938 353 183
BsaI GGTCTCn'nnnn_
Cuts at: 0 4699 6118 6792
Size: 4699 1419 674
Fragments arranged by size:
4699 1419 674
BsaAI yAC'GTr
Cuts at: 0 1040 5981 6792
Size: 1040 4941 811
Fragments arranged by size:
4941 1040 811
BsaBI GATnn'nnATC
Cuts at: 0 210 6792
Size: 210 6582
BsaHI Gr'CG_yC
Cuts at: 0 752 3280 6792
Size: 752 2528 3512
Fragments arranged by size:
3512 2528 752
BsaWI w'CCGG_w
Cuts at: 0 2100 6792
Size: 2100 4692
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1766 1796 4215 4245 6792
Size: 1766 30 2419 30 2547
Fragments arranged by size:
2547 2419 1766 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1112 1394 3518 4906 5431 6361 6436 6792
Size: 1112 282 2124 1388 525 930 75 356
Fragments arranged by size:
2124 1388 1112 930 525 356 282 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2621 5689 6627 6792
Size: 2621 3068 938 165
Fragments arranged by size:
3068 2621 938 165
BsiEI CG_ry'CG
Cuts at: 0 5434 6792
Size: 5434 1358
BsiHKAI G_wGCw'C
Cuts at: 0 1352 3379 6376 6792
Size: 1352 2027 2997 416
Fragments arranged by size:
2997 2027 1352 416
BsmI GAATG_Cn'
Cuts at: 0 3116 5661 6792
Size: 3116 2545 1131
Fragments arranged by size:
3116 2545 1131
Bsp1286I G_dGCh'C
Cuts at: 0 1352 2674 2801 3379 4521 6376 6792
Size: 1352 1322 127 578 1142 1855 416
Fragments arranged by size:
1855 1352 1322 1142 578 416 127
BspHI T'CATG_A
Cuts at: 0 1017 5211 6792
Size: 1017 4194 1581
Fragments arranged by size:
4194 1581 1017
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2427 2747 5061 6792
Size: 2427 320 2314 1731
Fragments arranged by size:
2427 2314 1731 320
BsrDI GCAATG_nn'
Cuts at: 0 6003 6792
Size: 6003 789
BsrFI r'CCGG_y
Cuts at: 0 2100 4614 6792
Size: 2100 2514 2178
Fragments arranged by size:
2514 2178 2100
BsrGI T'GTAC_A
Cuts at: 0 4483 5034 5755 6576 6792
Size: 4483 551 721 821 216
Fragments arranged by size:
4483 821 721 551 216
BssSI C'ACGA_G
Cuts at: 0 3434 6792
Size: 3434 3358
BstEII G'GTnAC_C
Cuts at: 0 1663 6792
Size: 1663 5129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2790 5350 6792
Size: 2790 2560 1442
Fragments arranged by size:
2790 2560 1442
BstYI r'GATC_y
Cuts at: 0 1228 2355 2400 2970 3469 3949 5046 6487
Size: 1228 1127 45 570 499 480 1097 1441
Cuts at: 6487 6792
Size: 305
Fragments arranged by size:
1441 1228 1127 1097 570 499 480 305
45
BstZ17I GTA'TAC
Cuts at: 0 880 5388 6792
Size: 880 4508 1404
Fragments arranged by size:
4508 1404 880
BtgI C'CryG_G
Cuts at: 0 2661 5343 6792
Size: 2661 2682 1449
Fragments arranged by size:
2682 2661 1449
BtsI GCAGTG_nn'
Cuts at: 0 388 1512 4739 6437 6615 6792
Size: 388 1124 3227 1698 178 177
Fragments arranged by size:
3227 1698 1124 388 178 177
ClaI AT'CG_AT
Cuts at: 0 4965 6792
Size: 4965 1827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3212 3247 6792
Size: 3212 35 3545
Fragments arranged by size:
3545 3212 35
DraI TTT'AAA
Cuts at: 0 708 2013 2967 3482 4464 6792
Size: 708 1305 954 515 982 2328
Fragments arranged by size:
2328 1305 982 954 708 515
DraIII CAC_nnn'GTG
Cuts at: 0 2794 6792
Size: 2794 3998
EarI CTCTTCn'nnn_
Cuts at: 0 77 169 2449 2888 5069 6208 6792
Size: 77 92 2280 439 2181 1139 584
Fragments arranged by size:
2280 2181 1139 584 439 92 77
EcoICRI GAG'CTC
Cuts at: 0 1350 6374 6792
Size: 1350 5024 418
Fragments arranged by size:
5024 1350 418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1187 1208 1226 1886 2595 3373 3821
Size: 1166 21 21 18 660 709 778 448
Cuts at: 3821 3884 3995 4127 4172 4397 4571 4796 5234
Size: 63 111 132 45 225 174 225 438
Cuts at: 5234 5246 5789 6390 6430 6586 6661 6792
Size: 12 543 601 40 156 75 131
Fragments arranged by size:
1166 778 709 660 601 543 448 438
225 225 174 156 132 131 111 75
63 45 40 21 21 18 12
EcoO109I rG'GnC_Cy
Cuts at: 0 2670 6792
Size: 2670 4122
EcoRI G'AATT_C
Cuts at: 0 271 1629 3822 3921 6792
Size: 271 1358 2193 99 2871
Fragments arranged by size:
2871 2193 1358 271 99
EcoRV GAT'ATC
Cuts at: 0 4260 6756 6792
Size: 4260 2496 36
Fragments arranged by size:
4260 2496 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 292 1171 1304 6792
Size: 292 879 133 5488
Fragments arranged by size:
5488 879 292 133
HaeII r_GCGC'y
Cuts at: 0 762 6792
Size: 762 6030
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2380 2412 3195 3227 4215 4247 5278 5310
Size: 2380 32 783 32 988 32 1031 32
Cuts at: 5310 5731 5763 6358 6390 6792
Size: 421 32 595 32 402
Fragments arranged by size:
2380 1031 988 783 595 421 402 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2222 3702 6792
Size: 2222 1480 3090
Fragments arranged by size:
3090 2222 1480
HindIII A'AGCT_T
Cuts at: 0 998 6792
Size: 998 5794
HpaI GTT'AAC
Cuts at: 0 3702 6792
Size: 3702 3090
MfeI C'AATT_G
Cuts at: 0 4444 6792
Size: 4444 2348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1880 3238 3358 3670 4960 5469 6792
Size: 1880 1358 120 312 1290 509 1323
Fragments arranged by size:
1880 1358 1323 1290 509 312 120
MslI CAynn'nnrTG
Cuts at: 0 833 1106 1441 3635 4197 4724 6725 6792
Size: 833 273 335 2194 562 527 2001 67
Fragments arranged by size:
2194 2001 833 562 527 335 273 67
MspA1I CmG'CkG
Cuts at: 0 1315 1919 6289 6792
Size: 1315 604 4370 503
Fragments arranged by size:
4370 1315 604 503
NaeI GCC'GGC
Cuts at: 0 4616 6792
Size: 4616 2176
NdeI CA'TA_TG
Cuts at: 0 340 6792
Size: 340 6452
NgoMIV G'CCGG_C
Cuts at: 0 4614 6792
Size: 4614 2178
NheI G'CTAG_C
Cuts at: 0 4647 6792
Size: 4647 2145
NsiI A_TGCA'T
Cuts at: 0 3183 6792
Size: 3183 3609
NspI r_CATG'y
Cuts at: 0 246 301 397 2182 2963 4484 5716 6792
Size: 246 55 96 1785 781 1521 1232 1076
Fragments arranged by size:
1785 1521 1232 1076 781 246 96 55
PciI A'CATG_T
Cuts at: 0 4480 6792
Size: 4480 2312
PflMI CCAn_nnn'nTGG
Cuts at: 0 2071 2833 3561 3813 6792
Size: 2071 762 728 252 2979
Fragments arranged by size:
2979 2071 762 728 252
PfoI T'CCnGG_A
Cuts at: 0 1580 6792
Size: 1580 5212
PmlI CAC'GTG
Cuts at: 0 5981 6792
Size: 5981 811
PshAI GACnn'nnGTC
Cuts at: 0 1371 6792
Size: 1371 5421
PsiI TTA'TAA
Cuts at: 0 1034 1805 3448 3995 4069 5843 6792
Size: 1034 771 1643 547 74 1774 949
Fragments arranged by size:
1774 1643 1034 949 771 547 74
PspOMI G'GGCC_C
Cuts at: 0 2670 6792
Size: 2670 4122
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2289 2321 6003 6035 6792
Size: 2289 32 3682 32 757
Fragments arranged by size:
3682 2289 757 32 32
PstI C_TGCA'G
Cuts at: 0 2424 4257 6432 6792
Size: 2424 1833 2175 360
Fragments arranged by size:
2424 2175 1833 360
PvuII CAG'CTG
Cuts at: 0 1315 1919 6289 6792
Size: 1315 604 4370 503
Fragments arranged by size:
4370 1315 604 503
SacI G_AGCT'C
Cuts at: 0 1352 6376 6792
Size: 1352 5024 416
Fragments arranged by size:
5024 1352 416
ScaI AGT'ACT
Cuts at: 0 703 799 1119 2331 6792
Size: 703 96 320 1212 4461
Fragments arranged by size:
4461 1212 703 320 96
SfcI C'TryA_G
Cuts at: 0 801 2420 2751 2860 2983 4253 4353 6020
Size: 801 1619 331 109 123 1270 100 1667
Cuts at: 6020 6428 6792
Size: 408 364
Fragments arranged by size:
1667 1619 1270 801 408 364 331 123
109 100
SgrAI Cr'CCGG_yG
Cuts at: 0 2100 6792
Size: 2100 4692
SmlI C'TyrA_G
Cuts at: 0 1816 2849 3751 3934 4927 6418 6792
Size: 1816 1033 902 183 993 1491 374
Fragments arranged by size:
1816 1491 1033 993 902 374 183
SnaBI TAC'GTA
Cuts at: 0 1040 6792
Size: 1040 5752
SpeI A'CTAG_T
Cuts at: 0 6532 6792
Size: 6532 260
SspI AAT'ATT
Cuts at: 0 1215 2385 6513 6792
Size: 1215 1170 4128 279
Fragments arranged by size:
4128 1215 1170 279
StuI AGG'CCT
Cuts at: 0 456 4581 6792
Size: 456 4125 2211
Fragments arranged by size:
4125 2211 456
StyI C'CwwG_G
Cuts at: 0 276 4341 6792
Size: 276 4065 2451
Fragments arranged by size:
4065 2451 276
SwaI ATTT'AAAT
Cuts at: 0 3482 6792
Size: 3482 3310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2205 2782 6792
Size: 2205 577 4010
Fragments arranged by size:
4010 2205 577
TatI w'GTAC_w
Cuts at: 0 701 797 1117 1936 2329 3124 4203 4483
Size: 701 96 320 819 393 795 1079 280
Cuts at: 4483 4627 5034 5755 5800 6576 6717 6792
Size: 144 407 721 45 776 141 75
Fragments arranged by size:
1079 819 795 776 721 701 407 393
320 280 144 141 96 75 45
XbaI T'CTAG_A
Cuts at: 0 5331 6792
Size: 5331 1461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5179 6469 6792
Size: 5179 1290 323
Fragments arranged by size:
5179 1290 323
XmnI GAAnn'nnTTC
Cuts at: 0 629 990 4421 6792
Size: 629 361 3431 2371
Fragments arranged by size:
3431 2371 629 361
ZraI GAC'GTC
Cuts at: 0 3281 6792
Size: 3281 3511
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaI ApoI AseI BaeI
BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI
BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI BstZ17I BtgI BtsI
ClaI CspCI DraI DraIII EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MslI MspA1I NaeI NdeI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PmlI PshAI PsiI
PspOMI PsrI PstI PvuII SacI ScaI SfcI SgrAI
SmlI SnaBI SpeI SspI StuI StyI SwaI TaqII
TatI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AhdI AleI ApaLI AscI AsiSI AvaI AvrII
BbeI BbvCI BglI BlpI BmgBI BseYI BsiWI BsmBI
BspEI BsrBI BssHII BstAPI BstBI Bsu36I BtgZI DrdI
EaeI EagI EciI EcoNI FseI FspI FspAI KasI
KpnI MluI MscI NarI NcoI NotI NruI PacI
PasI PmeI PpiI PpuMI PspXI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SmaI SphI SrfI TstI Tth111I XhoI XmaI