(Linear) (Six-base) MAPSORT of: KIAA0533.seq Check: 5053 from: 1 to: 11070 KIAA0533.orf With 182 enzymes: SgfI * July 15, 2010 18:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 976 991 1003 1324 1440 1670 1906 2581 Size: 976 15 12 321 116 230 236 675 Cuts at: 2581 2627 2875 3274 4971 5821 6439 11070 Size: 46 248 399 1697 850 618 4631 Fragments arranged by size: 4631 1697 976 850 675 618 399 321 248 236 230 116 46 15 12 AatII G_ACGT'C Cuts at: 0 714 3290 5558 7526 11070 Size: 714 2576 2268 1968 3544 Fragments arranged by size: 3544 2576 2268 1968 714 AccI GT'mk_AC Cuts at: 0 4148 8800 9440 11070 Size: 4148 4652 640 1630 Fragments arranged by size: 4652 4148 1630 640 Acc65I G'GTAC_C Cuts at: 0 8326 8639 11070 Size: 8326 313 2431 Fragments arranged by size: 8326 2431 313 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 111 2063 2685 5925 7305 7931 8965 9584 Size: 111 1952 622 3240 1380 626 1034 619 Cuts at: 9584 9756 11070 Size: 172 1314 Fragments arranged by size: 3240 1952 1380 1314 1034 626 622 619 172 111 AfeI AGC'GCT Cuts at: 0 376 1219 4906 5680 6421 8893 10255 11070 Size: 376 843 3687 774 741 2472 1362 815 Fragments arranged by size: 3687 2472 1362 843 815 774 741 376 AflIII A'CryG_T Cuts at: 0 1376 1708 2125 2405 4345 7643 8522 11070 Size: 1376 332 417 280 1940 3298 879 2548 Fragments arranged by size: 3298 2548 1940 1376 879 417 332 280 AgeI A'CCGG_T Cuts at: 0 1850 10997 11070 Size: 1850 9147 73 Fragments arranged by size: 9147 1850 73 AhdI GACnn_n'nnGTC Cuts at: 0 5112 11070 Size: 5112 5958 AleI CACnn'nnGTG Cuts at: 0 2128 3565 3808 3874 4027 7933 8770 10762 Size: 2128 1437 243 66 153 3906 837 1992 Cuts at: 10762 11070 Size: 308 Fragments arranged by size: 3906 2128 1992 1437 837 308 243 153 66 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 578 657 1914 5402 6058 7459 9010 9130 Size: 578 79 1257 3488 656 1401 1551 120 Cuts at: 9130 11070 Size: 1940 Fragments arranged by size: 3488 1940 1551 1401 1257 656 578 120 79 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2752 2784 11070 Size: 2752 32 8286 Fragments arranged by size: 8286 2752 32 AlwNI CAG_nnn'CTG Cuts at: 0 609 1146 1455 1975 3414 3707 3998 4050 Size: 609 537 309 520 1439 293 291 52 Cuts at: 4050 4208 4448 5894 5948 6347 6696 7012 7076 Size: 158 240 1446 54 399 349 316 64 Cuts at: 7076 7095 7254 7482 7626 7671 7695 7746 8013 Size: 19 159 228 144 45 24 51 267 Cuts at: 8013 8079 8311 8907 8964 9555 9779 9846 10466 Size: 66 232 596 57 591 224 67 620 Cuts at: 10466 10722 11057 11070 Size: 256 335 13 Fragments arranged by size: 1446 1439 620 609 596 591 537 520 399 349 335 316 309 293 291 267 256 240 232 228 224 159 158 144 67 66 64 57 54 52 51 45 24 19 13 ApaI G_GGCC'C Cuts at: 0 55 262 2569 2991 3402 4598 4813 5469 Size: 55 207 2307 422 411 1196 215 656 Cuts at: 5469 5670 6002 6609 6673 6984 6999 8305 8443 Size: 201 332 607 64 311 15 1306 138 Cuts at: 8443 9722 9817 10332 10509 10549 10876 11070 Size: 1279 95 515 177 40 327 194 Fragments arranged by size: 2307 1306 1279 1196 656 607 515 422 411 332 327 311 215 207 201 194 177 138 95 64 55 40 15 ApaLI G'TGCA_C Cuts at: 0 963 2504 3382 8113 8410 9875 11070 Size: 963 1541 878 4731 297 1465 1195 Fragments arranged by size: 4731 1541 1465 1195 963 878 297 ApoI r'AATT_y Cuts at: 0 726 11070 Size: 726 10344 AscI GG'CGCG_CC Cuts at: 0 9366 11070 Size: 9366 1704 AvaI C'yCGr_G Cuts at: 0 47 1786 1907 2308 2686 3464 3605 4096 Size: 47 1739 121 401 378 778 141 491 Cuts at: 4096 5056 5068 6402 6727 6770 6983 7049 7259 Size: 960 12 1334 325 43 213 66 210 Cuts at: 7259 8177 9222 9694 9723 9900 10010 10124 10222 Size: 918 1045 472 29 177 110 114 98 Cuts at: 10222 10331 10918 11070 Size: 109 587 152 Fragments arranged by size: 1739 1334 1045 960 918 778 587 491 472 401 378 325 213 210 177 152 141 121 114 110 109 98 66 47 43 29 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 8631 8664 11070 Size: 8631 33 2406 Fragments arranged by size: 8631 2406 33 BamHI G'GATC_C Cuts at: 0 2199 10307 11070 Size: 2199 8108 763 Fragments arranged by size: 8108 2199 763 BanI G'GyrC_C Cuts at: 0 163 718 991 1127 1153 1312 1567 1682 Size: 163 555 273 136 26 159 255 115 Cuts at: 1682 1853 2137 2377 2546 2727 2980 3567 3631 Size: 171 284 240 169 181 253 587 64 Cuts at: 3631 4459 4673 4986 5833 5965 6450 6544 6799 Size: 828 214 313 847 132 485 94 255 Cuts at: 6799 7774 8202 8326 8639 8842 9420 9595 9757 Size: 975 428 124 313 203 578 175 162 Cuts at: 9757 10799 10897 11070 Size: 1042 98 173 Fragments arranged by size: 1042 975 847 828 587 578 555 485 428 313 313 284 273 255 255 253 240 214 203 181 175 173 171 169 163 162 159 136 132 124 115 98 94 64 26 BanII G_rGCy'C Cuts at: 0 55 262 1583 1917 2244 2299 2569 2963 Size: 55 207 1321 334 327 55 270 394 Cuts at: 2963 2991 3402 3605 3830 3848 4076 4178 4305 Size: 28 411 203 225 18 228 102 127 Cuts at: 4305 4355 4481 4598 4613 4813 4918 5348 5469 Size: 50 126 117 15 200 105 430 121 Cuts at: 5469 5670 6002 6235 6326 6410 6609 6664 6673 Size: 201 332 233 91 84 199 55 9 Cuts at: 6673 6875 6984 6999 7202 7392 7686 8165 8305 Size: 202 109 15 203 190 294 479 140 Cuts at: 8305 8443 9701 9722 9817 10332 10509 10549 10876 Size: 138 1258 21 95 515 177 40 327 Cuts at: 10876 10937 11070 Size: 61 133 Fragments arranged by size: 1321 1258 515 479 430 411 394 334 332 327 327 294 270 233 228 225 207 203 203 202 201 200 199 190 177 140 138 133 127 126 121 117 109 105 102 95 91 84 61 55 55 55 50 40 28 21 18 15 15 9 BbeI G_GCGC'C Cuts at: 0 167 722 1131 3635 4677 5969 6548 11070 Size: 167 555 409 2504 1042 1292 579 4522 Fragments arranged by size: 4522 2504 1292 1042 579 555 409 167 BbsI GAAGACnn'nnnn_ Cuts at: 0 444 2784 3483 3855 6832 7311 8068 8616 Size: 444 2340 699 372 2977 479 757 548 Cuts at: 8616 9786 11070 Size: 1170 1284 Fragments arranged by size: 2977 2340 1284 1170 757 699 548 479 444 372 BbvCI CC'TCA_GC Cuts at: 0 7013 11070 Size: 7013 4057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 342 376 1325 1359 4312 4346 5833 5867 Size: 342 34 949 34 2953 34 1487 34 Cuts at: 5867 5998 6032 6708 6742 6771 6805 7594 7628 Size: 131 34 676 34 29 34 789 34 Cuts at: 7628 7641 7675 7866 7900 8251 8285 8832 8847 Size: 13 34 191 34 351 34 547 15 Cuts at: 8847 8866 8881 9777 9811 11070 Size: 19 15 896 34 1259 Fragments arranged by size: 2953 1487 1259 949 896 789 676 547 351 342 191 131 34 34 34 34 34 34 34 34 34 34 34 34 29 19 15 15 13 BciVI GTATCCnnnnn_n' Cuts at: 0 3462 4414 11070 Size: 3462 952 6656 Fragments arranged by size: 6656 3462 952 BclI T'GATC_A Cuts at: 0 3260 11070 Size: 3260 7810 BglI GCCn_nnn'nGGC Cuts at: 0 50 1151 1229 1691 1862 1961 2188 2375 Size: 50 1101 78 462 171 99 227 187 Cuts at: 2375 3535 5456 5624 5690 6362 6668 6847 7003 Size: 1160 1921 168 66 672 306 179 156 Cuts at: 7003 7465 7558 7597 8003 8128 8182 8584 9073 Size: 462 93 39 406 125 54 402 489 Cuts at: 9073 9717 9726 9940 9969 10359 11070 Size: 644 9 214 29 390 711 Fragments arranged by size: 1921 1160 1101 711 672 644 489 462 462 406 402 390 306 227 214 187 179 171 168 156 125 99 93 78 66 54 50 39 29 9 BglII A'GATC_T Cuts at: 0 5402 6011 11070 Size: 5402 609 5059 Fragments arranged by size: 5402 5059 609 BlpI GC'TnA_GC Cuts at: 0 3238 4966 6409 7066 8089 11070 Size: 3238 1728 1443 657 1023 2981 Fragments arranged by size: 3238 2981 1728 1443 1023 657 Bme1580I G_kGCm'C Cuts at: 0 55 262 671 967 994 1725 2135 2347 Size: 55 207 409 296 27 731 410 212 Cuts at: 2347 2380 2508 2569 2942 2991 3386 3402 3460 Size: 33 128 61 373 49 395 16 58 Cuts at: 3460 3572 3881 4097 4464 4598 4750 4813 4991 Size: 112 309 216 367 134 152 63 178 Cuts at: 4991 5057 5391 5469 5500 5538 5670 6002 6021 Size: 66 334 78 31 38 132 332 19 Cuts at: 6021 6116 6309 6358 6609 6673 6984 6999 8117 Size: 95 193 49 251 64 311 15 1118 Cuts at: 8117 8207 8305 8414 8443 8790 9480 9600 9722 Size: 90 98 109 29 347 690 120 122 Cuts at: 9722 9817 9879 10332 10509 10549 10876 10902 11070 Size: 95 62 453 177 40 327 26 168 Fragments arranged by size: 1118 731 690 453 410 409 395 373 367 347 334 332 327 311 309 296 251 216 212 207 193 178 177 168 152 134 132 128 122 120 112 109 98 95 95 90 78 66 64 63 62 61 58 55 49 49 40 38 33 31 29 27 26 19 16 15 BmgBI CAC'GTC Cuts at: 0 1272 1377 4059 10107 10760 11070 Size: 1272 105 2682 6048 653 310 Fragments arranged by size: 6048 2682 1272 653 310 105 BmrI ACTGGGnnnn_n' Cuts at: 0 2188 2359 2518 4434 4679 5799 6005 6149 Size: 2188 171 159 1916 245 1120 206 144 Cuts at: 6149 6285 6327 6823 8381 8424 8639 10085 11070 Size: 136 42 496 1558 43 215 1446 985 Fragments arranged by size: 2188 1916 1558 1446 1120 985 496 245 215 206 171 159 144 136 43 42 BmtI G_CTAG'C Cuts at: 0 10810 11070 Size: 10810 260 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 10150 11070 Size: 10150 920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 535 607 631 694 1658 2647 2653 Size: 430 105 72 24 63 964 989 6 Cuts at: 2653 2727 3202 3749 5260 5389 5458 6718 6727 Size: 74 475 547 1511 129 69 1260 9 Cuts at: 6727 7246 7381 7714 7753 7840 8077 8119 8494 Size: 519 135 333 39 87 237 42 375 Cuts at: 8494 9190 9646 9685 10072 10359 10573 10660 10861 Size: 696 456 39 387 287 214 87 201 Cuts at: 10861 11070 Size: 209 Fragments arranged by size: 1511 1260 989 964 696 547 519 475 456 430 387 375 333 287 237 214 209 201 135 129 105 87 87 74 72 69 63 42 39 39 24 9 6 Bpu10I CC'TnA_GC Cuts at: 0 4590 6329 6865 7013 7349 8226 8312 8452 Size: 4590 1739 536 148 336 877 86 140 Cuts at: 8452 10186 10931 11070 Size: 1734 745 139 Fragments arranged by size: 4590 1739 1734 877 745 536 336 148 140 139 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 8269 9292 9560 11070 Size: 8269 1023 268 1510 Fragments arranged by size: 8269 1510 1023 268 BsaI GGTCTCn'nnnn_ Cuts at: 0 2841 5149 5569 6767 10287 11070 Size: 2841 2308 420 1198 3520 783 Fragments arranged by size: 3520 2841 2308 1198 783 420 BsaAI yAC'GTr Cuts at: 0 459 751 2247 2406 3027 4346 5040 7646 Size: 459 292 1496 159 621 1319 694 2606 Cuts at: 7646 11070 Size: 3424 Fragments arranged by size: 3424 2606 1496 1319 694 621 459 292 159 BsaBI GATnn'nnATC Cuts at: 0 5227 11070 Size: 5227 5843 BsaHI Gr'CG_yC Cuts at: 0 164 711 719 1128 1205 1420 2537 3287 Size: 164 547 8 409 77 215 1117 750 Cuts at: 3287 3632 4674 5555 5633 5966 6545 7178 7523 Size: 345 1042 881 78 333 579 633 345 Cuts at: 7523 9182 9496 10696 11070 Size: 1659 314 1200 374 Fragments arranged by size: 1659 1200 1117 1042 881 750 633 579 547 409 374 345 345 333 314 215 164 78 77 8 BsaWI w'CCGG_w Cuts at: 0 1850 4417 6656 6938 9247 10304 10443 10663 Size: 1850 2567 2239 282 2309 1057 139 220 Cuts at: 10663 10997 11070 Size: 334 73 Fragments arranged by size: 2567 2309 2239 1850 1057 334 282 220 139 73 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 364 394 674 704 2752 2782 2842 2872 Size: 364 30 280 30 2048 30 60 30 Cuts at: 2872 3175 3205 4677 4707 8646 8676 9242 9272 Size: 303 30 1472 30 3939 30 566 30 Cuts at: 9272 9427 9457 10267 10297 11070 Size: 155 30 810 30 773 Fragments arranged by size: 3939 2048 1472 810 773 566 364 303 280 155 60 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 124 211 558 2650 2863 3124 3592 4186 Size: 124 87 347 2092 213 261 468 594 Cuts at: 4186 4591 5356 5397 6541 7096 7369 8734 9721 Size: 405 765 41 1144 555 273 1365 987 Cuts at: 9721 10123 10126 10995 11070 Size: 402 3 869 75 Fragments arranged by size: 2092 1365 1144 987 869 765 594 555 468 405 402 347 273 261 213 124 87 75 41 3 BseYI C'CCAG_C Cuts at: 0 87 807 2014 2299 2911 3110 3404 3686 Size: 87 720 1207 285 612 199 294 282 Cuts at: 3686 3777 3885 4237 5159 5451 5716 5883 7124 Size: 91 108 352 922 292 265 167 1241 Cuts at: 7124 7482 7685 7746 7784 7943 8072 8427 9093 Size: 358 203 61 38 159 129 355 666 Cuts at: 9093 10292 10385 10902 11061 11070 Size: 1199 93 517 159 9 Fragments arranged by size: 1241 1207 1199 922 720 666 612 517 358 355 352 294 292 285 282 265 203 199 167 159 159 129 108 93 91 87 61 38 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 300 627 1698 2038 2106 2596 2862 Size: 118 182 327 1071 340 68 490 266 Cuts at: 2862 3075 3252 3409 3535 3892 4674 4825 4848 Size: 213 177 157 126 357 782 151 23 Cuts at: 4848 5045 5131 6042 6598 7075 7315 7615 7694 Size: 197 86 911 556 477 240 300 79 Cuts at: 7694 7957 8254 9327 9820 10471 10966 11070 Size: 263 297 1073 493 651 495 104 Fragments arranged by size: 1073 1071 911 782 651 556 495 493 490 477 357 340 327 300 297 266 263 240 213 197 182 177 157 151 126 118 104 86 79 68 23 BsiEI CG_ry'CG Cuts at: 0 499 537 1002 1809 3173 3966 5799 6224 Size: 499 38 465 807 1364 793 1833 425 Cuts at: 6224 7116 8804 11070 Size: 892 1688 2266 Fragments arranged by size: 2266 1833 1688 1364 892 807 793 499 465 425 38 BsiHKAI G_wGCw'C Cuts at: 0 967 1583 2508 3017 3386 4076 4481 4613 Size: 967 616 925 509 369 690 405 132 Cuts at: 4613 4918 4972 5348 6721 6875 7202 8117 8165 Size: 305 54 376 1373 154 327 915 48 Cuts at: 8165 8414 8936 9701 9868 9879 10382 10835 11070 Size: 249 522 765 167 11 503 453 235 Fragments arranged by size: 1373 967 925 915 765 690 616 522 509 503 453 405 376 369 327 305 249 235 167 154 132 54 48 11 BsmI GAATG_Cn' Cuts at: 0 1993 11070 Size: 1993 9077 BsmBI CGTCTCn'nnnn_ Cuts at: 0 6676 11070 Size: 6676 4394 Bsp1286I G_dGCh'C Cuts at: 0 55 262 671 967 994 1583 1725 1917 Size: 55 207 409 296 27 589 142 192 Cuts at: 1917 2135 2244 2299 2347 2380 2508 2569 2942 Size: 218 109 55 48 33 128 61 373 Cuts at: 2942 2963 2991 3017 3386 3402 3460 3572 3605 Size: 21 28 26 369 16 58 112 33 Cuts at: 3605 3830 3848 3881 4076 4097 4178 4305 4355 Size: 225 18 33 195 21 81 127 50 Cuts at: 4355 4464 4481 4598 4613 4750 4813 4918 4972 Size: 109 17 117 15 137 63 105 54 Cuts at: 4972 4991 5057 5348 5391 5469 5500 5538 5670 Size: 19 66 291 43 78 31 38 132 Cuts at: 5670 6002 6021 6116 6235 6309 6326 6358 6410 Size: 332 19 95 119 74 17 32 52 Cuts at: 6410 6609 6664 6673 6721 6875 6984 6999 7202 Size: 199 55 9 48 154 109 15 203 Cuts at: 7202 7392 7686 8117 8165 8207 8305 8414 8443 Size: 190 294 431 48 42 98 109 29 Cuts at: 8443 8790 8936 9480 9600 9701 9722 9817 9868 Size: 347 146 544 120 101 21 95 51 Cuts at: 9868 9879 10332 10382 10509 10549 10835 10876 10902 Size: 11 453 50 127 40 286 41 26 Cuts at: 10902 10937 11070 Size: 35 133 Fragments arranged by size: 589 544 453 431 409 373 369 347 332 296 294 291 286 225 218 207 203 199 195 192 190 154 146 142 137 133 132 128 127 127 120 119 117 112 109 109 109 109 105 101 98 95 95 81 78 74 66 63 61 58 55 55 55 54 52 51 50 50 48 48 48 43 42 41 40 38 35 33 33 33 32 31 29 28 27 26 26 21 21 21 19 19 18 17 17 16 15 15 11 9 BspEI T'CCGG_A Cuts at: 0 6656 6938 11070 Size: 6656 282 4132 Fragments arranged by size: 6656 4132 282 BspHI T'CATG_A Cuts at: 0 5348 5696 11070 Size: 5348 348 5374 Fragments arranged by size: 5374 5348 348 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 196 976 991 1003 1324 1440 1670 1906 Size: 196 780 15 12 321 116 230 236 Cuts at: 1906 2581 2618 2627 2846 2875 3274 4696 4971 Size: 675 37 9 219 29 399 1422 275 Cuts at: 4971 5039 5821 6439 6650 7157 8303 8612 9362 Size: 68 782 618 211 507 1146 309 750 Cuts at: 9362 10707 11070 Size: 1345 363 Fragments arranged by size: 1422 1345 1146 782 780 750 675 618 507 399 363 321 309 275 236 230 219 211 196 116 68 37 29 15 12 9 BsrBI CCG'CTC Cuts at: 0 519 591 1241 1368 2828 4998 5847 6254 Size: 519 72 650 127 1460 2170 849 407 Cuts at: 6254 6960 7974 8792 9132 9426 11070 Size: 706 1014 818 340 294 1644 Fragments arranged by size: 2170 1644 1460 1014 849 818 706 650 519 407 340 294 127 72 BsrDI GCAATG_nn' Cuts at: 0 360 5484 8165 9630 10197 11070 Size: 360 5124 2681 1465 567 873 Fragments arranged by size: 5124 2681 1465 873 567 360 BsrFI r'CCGG_y Cuts at: 0 268 854 1124 1201 1346 1462 1804 1850 Size: 268 586 270 77 145 116 342 46 Cuts at: 1850 2093 2182 2380 2486 2543 3961 4103 4976 Size: 243 89 198 106 57 1418 142 873 Cuts at: 4976 5244 6219 6541 6796 7115 7135 8845 9337 Size: 268 975 322 255 319 20 1710 492 Cuts at: 9337 10358 10802 10997 11070 Size: 1021 444 195 73 Fragments arranged by size: 1710 1418 1021 975 873 586 492 444 342 322 319 270 268 268 255 243 198 195 145 142 116 106 89 77 73 57 46 20 BsrGI T'GTAC_A Cuts at: 0 3437 8348 9143 11070 Size: 3437 4911 795 1927 Fragments arranged by size: 4911 3437 1927 795 BssHII G'CGCG_C Cuts at: 0 208 1586 9366 11070 Size: 208 1378 7780 1704 Fragments arranged by size: 7780 1704 1378 208 BssSI C'ACGA_G Cuts at: 0 4129 4738 4993 6565 7129 8914 8992 11070 Size: 4129 609 255 1572 564 1785 78 2078 Fragments arranged by size: 4129 2078 1785 1572 609 564 255 78 BstAPI GCAn_nnn'nTGC Cuts at: 0 1455 1514 1979 2619 3414 3449 4880 4986 Size: 1455 59 465 640 795 35 1431 106 Cuts at: 4986 5807 5894 6065 6347 6450 7413 7593 7848 Size: 821 87 171 282 103 963 180 255 Cuts at: 7848 7956 8907 10722 10897 11070 Size: 108 951 1815 175 173 Fragments arranged by size: 1815 1455 1431 963 951 821 795 640 465 282 255 180 175 173 171 108 106 103 87 59 35 BstEII G'GTnAC_C Cuts at: 0 846 5923 10446 11070 Size: 846 5077 4523 624 Fragments arranged by size: 5077 4523 846 624 BstXI CCAn_nnnn'nTGG Cuts at: 0 1759 1930 2748 3118 3810 4390 4549 5481 Size: 1759 171 818 370 692 580 159 932 Cuts at: 5481 5995 6358 6597 7902 8371 10050 10518 11070 Size: 514 363 239 1305 469 1679 468 552 Fragments arranged by size: 1759 1679 1305 932 818 692 580 552 514 469 468 370 363 239 171 159 BstYI r'GATC_y Cuts at: 0 2199 3756 5381 5402 6011 8253 10307 11070 Size: 2199 1557 1625 21 609 2242 2054 763 Fragments arranged by size: 2242 2199 2054 1625 1557 763 609 21 Bsu36I CC'TnA_GG Cuts at: 0 1115 2213 2338 2651 3700 5045 9556 9713 Size: 1115 1098 125 313 1049 1345 4511 157 Cuts at: 9713 10000 11070 Size: 287 1070 Fragments arranged by size: 4511 1345 1115 1098 1070 1049 313 287 157 125 BtgI C'CryG_G Cuts at: 0 263 402 524 842 906 941 1030 1869 Size: 263 139 122 318 64 35 89 839 Cuts at: 1869 2897 3227 3627 3857 3941 4704 4898 5183 Size: 1028 330 400 230 84 763 194 285 Cuts at: 5183 5274 5598 5973 6281 6596 6761 7856 8106 Size: 91 324 375 308 315 165 1095 250 Cuts at: 8106 8248 8812 9137 9261 9381 9387 9629 10868 Size: 142 564 325 124 120 6 242 1239 Cuts at: 10868 10937 11034 11070 Size: 69 97 36 Fragments arranged by size: 1239 1095 1028 839 763 564 400 375 330 325 324 318 315 308 285 263 250 242 230 194 165 142 139 124 122 120 97 91 89 84 69 64 36 35 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 156 941 1811 6618 11070 Size: 156 785 870 4807 4452 Fragments arranged by size: 4807 4452 870 785 156 BtsI GCAGTG_nn' Cuts at: 0 967 1973 2031 2089 3282 3311 3458 4206 Size: 967 1006 58 58 1193 29 147 748 Cuts at: 4206 4748 4789 6114 6264 7178 7399 7706 8030 Size: 542 41 1325 150 914 221 307 324 Cuts at: 8030 8276 9037 10142 10615 11048 11070 Size: 246 761 1105 473 433 22 Fragments arranged by size: 1325 1193 1105 1006 967 914 761 748 542 473 433 324 307 246 221 150 147 58 58 41 29 22 ClaI AT'CG_AT Cuts at: 0 362 8459 11070 Size: 362 8097 2611 Fragments arranged by size: 8097 2611 362 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 9756 9791 11070 Size: 9756 35 1279 Fragments arranged by size: 9756 1279 35 DraIII CAC_nnn'GTG Cuts at: 0 34 2353 3449 5807 6065 6510 8046 8418 Size: 34 2319 1096 2358 258 445 1536 372 Cuts at: 8418 10869 11070 Size: 2451 201 Fragments arranged by size: 2451 2358 2319 1536 1096 445 372 258 201 34 DrdI GACnn_nn'nnGTC Cuts at: 0 844 1425 8620 11070 Size: 844 581 7195 2450 Fragments arranged by size: 7195 2450 844 581 EaeI y'GGCC_r Cuts at: 0 155 266 496 534 909 1086 1464 1643 Size: 155 111 230 38 375 177 378 179 Cuts at: 1643 1806 2382 2412 2750 2861 3170 3377 3963 Size: 163 576 30 338 111 309 207 586 Cuts at: 3963 4392 4641 5262 5360 5444 5471 5796 6221 Size: 429 249 621 98 84 27 325 425 Cuts at: 6221 6753 6794 6839 6902 6947 7097 7310 7617 Size: 532 41 45 63 45 150 213 307 Cuts at: 7617 7697 8081 8148 8517 8608 8972 9065 9176 Size: 80 384 67 369 91 364 93 111 Cuts at: 9176 9384 10194 10266 10418 10791 10940 11070 Size: 208 810 72 152 373 149 130 Fragments arranged by size: 810 621 586 576 532 429 425 384 378 375 373 369 364 338 325 309 307 249 230 213 208 207 179 177 163 155 152 150 149 130 111 111 111 98 93 91 84 80 72 67 63 45 45 41 38 30 27 EagI C'GGCC_G Cuts at: 0 496 534 1806 3170 3963 5796 6221 11070 Size: 496 38 1272 1364 793 1833 425 4849 Fragments arranged by size: 4849 1833 1364 1272 793 496 425 38 EarI CTCTTCn'nnn_ Cuts at: 0 4291 5398 5572 5944 7712 8941 9244 10144 Size: 4291 1107 174 372 1768 1229 303 900 Cuts at: 10144 10186 10468 11070 Size: 42 282 602 Fragments arranged by size: 4291 1768 1229 1107 900 602 372 303 282 174 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 766 7651 8127 10747 11070 Size: 766 6885 476 2620 323 Fragments arranged by size: 6885 2620 766 476 323 EcoICRI GAG'CTC Cuts at: 0 1581 4074 4479 4611 4916 5346 6873 7200 Size: 1581 2493 405 132 305 430 1527 327 Cuts at: 7200 8163 9699 11070 Size: 963 1536 1371 Fragments arranged by size: 2493 1581 1536 1527 1371 963 430 405 327 305 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 111 430 535 607 631 694 1658 2063 Size: 111 319 105 72 24 63 964 405 Cuts at: 2063 2647 2653 2685 2727 3202 3749 5260 5389 Size: 584 6 32 42 475 547 1511 129 Cuts at: 5389 5458 5925 6718 6727 7246 7305 7381 7714 Size: 69 467 793 9 519 59 76 333 Cuts at: 7714 7753 7840 7931 8077 8119 8494 8965 9190 Size: 39 87 91 146 42 375 471 225 Cuts at: 9190 9584 9646 9685 9756 10072 10359 10573 10660 Size: 394 62 39 71 316 287 214 87 Cuts at: 10660 10861 11070 Size: 201 209 Fragments arranged by size: 1511 964 793 584 547 519 475 471 467 405 394 375 333 319 316 287 225 214 209 201 146 129 111 105 91 87 87 76 72 71 69 63 62 59 42 42 39 39 32 24 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 157 445 573 1667 2212 4168 6124 6458 Size: 157 288 128 1094 545 1956 1956 334 Cuts at: 6458 6730 7078 7153 7342 7630 8017 9025 9529 Size: 272 348 75 189 288 387 1008 504 Cuts at: 9529 11070 Size: 1541 Fragments arranged by size: 1956 1956 1541 1094 1008 545 504 387 348 334 288 288 272 189 157 128 75 EcoO109I rG'GnC_Cy Cuts at: 0 51 52 201 234 258 259 837 1323 Size: 51 1 149 33 24 1 578 486 Cuts at: 1323 1499 1524 2565 2566 2988 3186 3399 4047 Size: 176 25 1041 1 422 198 213 648 Cuts at: 4047 4339 4445 4594 4809 4810 4838 5085 5129 Size: 292 106 149 215 1 28 247 44 Cuts at: 5129 5465 5466 5666 5999 6286 6670 6767 6987 Size: 336 1 200 333 287 384 97 220 Cuts at: 6987 6995 6996 7623 7668 7781 7995 8010 8301 Size: 8 1 627 45 113 214 15 291 Cuts at: 8301 8440 8750 8808 9285 9718 9719 9910 10328 Size: 139 310 58 477 433 1 191 418 Cuts at: 10328 10496 10505 10506 10546 10719 10873 10923 11070 Size: 168 9 1 40 173 154 50 147 Fragments arranged by size: 1041 648 627 578 486 477 433 422 418 384 336 333 310 292 291 287 247 220 215 214 213 200 198 191 176 173 168 154 149 149 147 139 113 106 97 58 51 50 45 44 40 33 28 25 24 15 9 8 1 1 1 1 1 1 1 1 EcoRV GAT'ATC Cuts at: 0 879 11070 Size: 879 10191 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2457 11070 Size: 2457 8613 FseI GG_CCGG'CC Cuts at: 0 1808 7139 11070 Size: 1808 5331 3931 Fragments arranged by size: 5331 3931 1808 FspI TGC'GCA Cuts at: 0 4212 5425 8242 11070 Size: 4212 1213 2817 2828 Fragments arranged by size: 4212 2828 2817 1213 HaeII r_GCGC'y Cuts at: 0 85 167 378 722 832 1131 1221 3064 Size: 85 82 211 344 110 299 90 1843 Cuts at: 3064 3635 4677 4908 5682 5723 5969 6150 6348 Size: 571 1042 231 774 41 246 181 198 Cuts at: 6348 6423 6548 7224 7368 8226 8895 9338 9409 Size: 75 125 676 144 858 669 443 71 Cuts at: 9409 9467 10180 10257 11070 Size: 58 713 77 813 Fragments arranged by size: 1843 1042 858 813 774 713 676 669 571 443 344 299 246 231 211 198 181 144 125 110 90 85 82 77 75 71 58 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 567 599 1054 1086 2842 2874 4675 4707 Size: 567 32 455 32 1756 32 1801 32 Cuts at: 4707 5098 5130 6862 6894 8534 8566 9242 9274 Size: 391 32 1732 32 1640 32 676 32 Cuts at: 9274 11070 Size: 1796 Fragments arranged by size: 1801 1796 1756 1732 1640 676 567 455 391 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 426 1209 2805 7527 8801 11070 Size: 426 783 1596 4722 1274 2269 Fragments arranged by size: 4722 2269 1596 1274 783 426 KasI G'GCGC_C Cuts at: 0 163 718 1127 3631 4673 5965 6544 11070 Size: 163 555 409 2504 1042 1292 579 4526 Fragments arranged by size: 4526 2504 1292 1042 579 555 409 163 KpnI G_GTAC'C Cuts at: 0 8330 8643 11070 Size: 8330 313 2427 Fragments arranged by size: 8330 2427 313 MfeI C'AATT_G Cuts at: 0 6787 11070 Size: 6787 4283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1322 5786 7589 11070 Size: 1322 4464 1803 3481 Fragments arranged by size: 4464 3481 1803 1322 MscI TGG'CCA Cuts at: 0 2752 5264 5362 5446 5473 6904 7099 7312 Size: 2752 2512 98 84 27 1431 195 213 Cuts at: 7312 7619 7699 8083 8519 9386 10196 10793 11070 Size: 307 80 384 436 867 810 597 277 Fragments arranged by size: 2752 2512 1431 867 810 597 436 384 307 277 213 195 98 84 80 27 MslI CAynn'nnrTG Cuts at: 0 917 2128 2821 2935 3565 3808 3874 4027 Size: 917 1211 693 114 630 243 66 153 Cuts at: 4027 4382 5353 7933 8386 8701 8770 10516 10762 Size: 355 971 2580 453 315 69 1746 246 Cuts at: 10762 10942 11070 Size: 180 128 Fragments arranged by size: 2580 1746 1211 971 917 693 630 453 355 315 246 243 180 153 128 114 69 66 MspA1I CmG'CkG Cuts at: 0 63 396 404 744 896 1004 1283 1655 Size: 63 333 8 340 152 108 279 372 Cuts at: 1655 1811 1952 2018 2090 2270 2324 2330 2483 Size: 156 141 66 72 180 54 6 153 Cuts at: 2483 2705 3072 3396 3614 3723 3741 3781 3808 Size: 222 367 324 218 109 18 40 27 Cuts at: 3808 4409 4640 4679 4718 4880 5163 5283 5289 Size: 601 231 39 39 162 283 120 6 Cuts at: 5289 5376 5615 5735 5915 6005 6110 6158 6368 Size: 87 239 120 180 90 105 48 210 Cuts at: 6368 6389 6413 6696 6933 7012 7065 7482 7491 Size: 21 24 283 237 79 53 417 9 Cuts at: 7491 7818 7947 8076 8238 8427 8488 8760 8927 Size: 327 129 129 162 189 61 272 167 Cuts at: 8927 9216 9804 10271 10463 10580 10894 11070 Size: 289 588 467 192 117 314 176 Fragments arranged by size: 601 588 467 417 372 367 340 333 327 324 314 289 283 283 279 272 239 237 231 222 218 210 192 189 180 180 176 167 162 162 156 153 152 141 129 129 120 120 117 109 108 105 90 87 79 72 66 63 61 54 53 48 40 39 39 27 24 21 18 9 8 6 6 NaeI GCC'GGC Cuts at: 0 270 1348 1464 1806 2095 3963 5246 6543 Size: 270 1078 116 342 289 1868 1283 1297 Cuts at: 6543 6798 7137 9339 11070 Size: 255 339 2202 1731 Fragments arranged by size: 2202 1868 1731 1297 1283 1078 342 339 289 270 255 116 NarI GG'CG_CC Cuts at: 0 164 719 1128 3632 4674 5966 6545 11070 Size: 164 555 409 2504 1042 1292 579 4525 Fragments arranged by size: 4525 2504 1292 1042 579 555 409 164 NcoI C'CATG_G Cuts at: 0 524 1869 4704 5183 5598 6281 6596 7856 Size: 524 1345 2835 479 415 683 315 1260 Cuts at: 7856 9381 10937 11070 Size: 1525 1556 133 Fragments arranged by size: 2835 1556 1525 1345 1260 683 524 479 415 315 133 NgoMIV G'CCGG_C Cuts at: 0 268 1346 1462 1804 2093 3961 5244 6541 Size: 268 1078 116 342 289 1868 1283 1297 Cuts at: 6541 6796 7135 9337 11070 Size: 255 339 2202 1733 Fragments arranged by size: 2202 1868 1733 1297 1283 1078 342 339 289 268 255 116 NheI G'CTAG_C Cuts at: 0 10806 11070 Size: 10806 264 NotI GC'GGCC_GC Cuts at: 0 5796 11070 Size: 5796 5274 NspI r_CATG'y Cuts at: 0 1712 1895 2129 7294 7894 8526 9987 11070 Size: 1712 183 234 5165 600 632 1461 1083 Fragments arranged by size: 5165 1712 1461 1083 632 600 234 183 PasI CC'CwG_GG Cuts at: 0 1503 2562 4218 6358 6666 8581 9288 10500 Size: 1503 1059 1656 2140 308 1915 707 1212 Cuts at: 10500 11070 Size: 570 Fragments arranged by size: 2140 1915 1656 1503 1212 1059 707 570 308 PciI A'CATG_T Cuts at: 0 1708 2125 8522 11070 Size: 1708 417 6397 2548 Fragments arranged by size: 6397 2548 1708 417 PflMI CCAn_nnn'nTGG Cuts at: 0 819 1146 3369 4563 6510 6602 6762 6792 Size: 819 327 2223 1194 1947 92 160 30 Cuts at: 6792 7095 8013 8079 10869 11070 Size: 303 918 66 2790 201 Fragments arranged by size: 2790 2223 1947 1194 918 819 327 303 201 160 92 66 30 PfoI T'CCnGG_A Cuts at: 0 2603 3017 4166 4604 5237 6521 7517 7730 Size: 2603 414 1149 438 633 1284 996 213 Cuts at: 7730 8096 8552 11070 Size: 366 456 2518 Fragments arranged by size: 2603 2518 1284 1149 996 633 456 438 414 366 213 PmlI CAC'GTG Cuts at: 0 459 2247 2406 4346 5040 7646 11070 Size: 459 1788 159 1940 694 2606 3424 Fragments arranged by size: 3424 2606 1940 1788 694 459 159 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2752 2784 11070 Size: 2752 32 8286 Fragments arranged by size: 8286 2752 32 PpuMI rG'GwC_Cy Cuts at: 0 234 837 1323 1499 1524 4838 5129 6286 Size: 234 603 486 176 25 3314 291 1157 Cuts at: 6286 6767 6987 7781 8010 8750 8808 10719 11070 Size: 481 220 794 229 740 58 1911 351 Fragments arranged by size: 3314 1911 1157 794 740 603 486 481 351 291 234 229 220 176 58 25 PshAI GACnn'nnGTC Cuts at: 0 2536 11070 Size: 2536 8534 PspOMI G'GGCC_C Cuts at: 0 51 258 2565 2987 3398 4594 4809 5465 Size: 51 207 2307 422 411 1196 215 656 Cuts at: 5465 5666 5998 6605 6669 6980 6995 8301 8439 Size: 201 332 607 64 311 15 1306 138 Cuts at: 8439 9718 9813 10328 10505 10545 10872 11070 Size: 1279 95 515 177 40 327 198 Fragments arranged by size: 2307 1306 1279 1196 656 607 515 422 411 332 327 311 215 207 201 198 177 138 95 64 51 40 15 PspXI vC'TCGA_Gb Cuts at: 0 2686 11070 Size: 2686 8384 PstI C_TGCA'G Cuts at: 0 962 1489 1768 1776 2329 2578 3071 4444 Size: 962 527 279 8 553 249 493 1373 Cuts at: 4444 5213 5288 5381 5914 6343 6436 6647 7032 Size: 769 75 93 533 429 93 211 385 Cuts at: 7032 7280 7409 7589 7631 7952 8300 8448 9044 Size: 248 129 180 42 321 348 148 596 Cuts at: 9044 9530 9839 9926 10893 11070 Size: 486 309 87 967 177 Fragments arranged by size: 1373 967 962 769 596 553 533 527 493 486 429 385 348 321 309 279 249 248 211 180 177 148 129 93 93 87 75 42 8 PvuII CAG'CTG Cuts at: 0 2018 2090 2330 2483 2705 3072 3614 3781 Size: 2018 72 240 153 222 367 542 167 Cuts at: 3781 5283 5289 5376 5735 6696 6933 7012 7065 Size: 1502 6 87 359 961 237 79 53 Cuts at: 7065 7482 7491 7818 7947 8076 8238 8427 8488 Size: 417 9 327 129 129 162 189 61 Cuts at: 8488 8760 9216 10894 11070 Size: 272 456 1678 176 Fragments arranged by size: 2018 1678 1502 961 542 456 417 367 359 327 272 240 237 222 189 176 167 162 153 129 129 87 79 72 61 53 9 6 SacI G_AGCT'C Cuts at: 0 1583 4076 4481 4613 4918 5348 6875 7202 Size: 1583 2493 405 132 305 430 1527 327 Cuts at: 7202 8165 9701 11070 Size: 963 1536 1369 Fragments arranged by size: 2493 1583 1536 1527 1369 963 430 405 327 305 132 SacII CC_GC'GG Cuts at: 0 405 11070 Size: 405 10665 SalI G'TCGA_C Cuts at: 0 8799 11070 Size: 8799 2271 SanDI GG'GwC_CC Cuts at: 0 837 1499 5129 6286 6767 8808 11070 Size: 837 662 3630 1157 481 2041 2262 Fragments arranged by size: 3630 2262 2041 1157 837 662 481 SapI GCTCTTCn'nnn_ Cuts at: 0 5944 8941 9244 10186 11070 Size: 5944 2997 303 942 884 Fragments arranged by size: 5944 2997 942 884 303 SbfI CC_TGCA'GG Cuts at: 0 1776 7280 7589 8300 11070 Size: 1776 5504 309 711 2770 Fragments arranged by size: 5504 2770 1776 711 309 ScaI AGT'ACT Cuts at: 0 304 655 11070 Size: 304 351 10415 Fragments arranged by size: 10415 351 304 SexAI A'CCwGG_T Cuts at: 0 5820 11070 Size: 5820 5250 SfcI C'TryA_G Cuts at: 0 958 1485 1764 1772 2325 2574 3067 4149 Size: 958 527 279 8 553 249 493 1082 Cuts at: 4149 4334 4440 5209 5284 5377 5910 6339 6432 Size: 185 106 769 75 93 533 429 93 Cuts at: 6432 6643 7028 7276 7405 7572 7585 7627 7948 Size: 211 385 248 129 167 13 42 321 Cuts at: 7948 8296 8444 8922 9040 9526 9547 9618 9835 Size: 348 148 478 118 486 21 71 217 Cuts at: 9835 9922 10889 11070 Size: 87 967 181 Fragments arranged by size: 1082 967 958 769 553 533 527 493 486 478 429 385 348 321 279 249 248 217 211 185 181 167 148 129 118 106 93 93 87 75 71 42 21 13 8 SfiI GGCCn_nnn'nGGCC Cuts at: 0 50 7465 7558 8003 11070 Size: 50 7415 93 445 3067 Fragments arranged by size: 7415 3067 445 93 50 SfoI GGC'GCC Cuts at: 0 165 720 1129 3633 4675 5967 6546 11070 Size: 165 555 409 2504 1042 1292 579 4524 Fragments arranged by size: 4524 2504 1292 1042 579 555 409 165 SgrAI Cr'CCGG_yG Cuts at: 0 1201 11070 Size: 1201 9869 SmaI CCC'GGG Cuts at: 0 49 3466 3607 6404 6985 7051 7261 8179 Size: 49 3417 141 2797 581 66 210 918 Cuts at: 8179 9902 10333 11070 Size: 1723 431 737 Fragments arranged by size: 3417 2797 1723 918 737 581 431 210 141 66 49 SmlI C'TyrA_G Cuts at: 0 2686 8284 9307 9575 11070 Size: 2686 5598 1023 268 1495 Fragments arranged by size: 5598 2686 1495 1023 268 SphI G_CATG'C Cuts at: 0 1895 7294 9987 11070 Size: 1895 5399 2693 1083 Fragments arranged by size: 5399 2693 1895 1083 SrfI GCCC'GGGC Cuts at: 0 10333 11070 Size: 10333 737 StuI AGG'CCT Cuts at: 0 2419 3088 6094 7561 8781 9743 9943 10130 Size: 2419 669 3006 1467 1220 962 200 187 Cuts at: 10130 10220 11070 Size: 90 850 Fragments arranged by size: 3006 2419 1467 1220 962 850 669 200 187 90 StyI C'CwwG_G Cuts at: 0 524 761 1528 1563 1869 4704 4833 5183 Size: 524 237 767 35 306 2835 129 350 Cuts at: 5183 5598 6281 6596 7856 7895 8561 9381 10937 Size: 415 683 315 1260 39 666 820 1556 Cuts at: 10937 11070 Size: 133 Fragments arranged by size: 2835 1556 1260 820 767 683 666 524 415 350 315 306 237 133 129 39 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 498 1585 2341 2575 2925 3886 4947 5818 Size: 498 1087 756 234 350 961 1061 871 Cuts at: 5818 7558 7694 8374 8406 8419 11070 Size: 1740 136 680 32 13 2651 Fragments arranged by size: 2651 1740 1087 1061 961 871 756 680 498 350 234 136 32 13 TatI w'GTAC_w Cuts at: 0 302 413 653 3437 4125 5075 8348 9143 Size: 302 111 240 2784 688 950 3273 795 Cuts at: 9143 11070 Size: 1927 Fragments arranged by size: 3273 2784 1927 950 795 688 302 240 111 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 364 396 674 706 3084 3116 3175 3207 Size: 364 32 278 32 2378 32 59 32 Cuts at: 3207 3530 3562 4496 4528 7630 7662 8032 8064 Size: 323 32 934 32 3102 32 370 32 Cuts at: 8064 8646 8678 10267 10299 11070 Size: 582 32 1589 32 771 Fragments arranged by size: 3102 2378 1589 934 771 582 370 364 323 278 59 32 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1541 8620 11070 Size: 1541 7079 2450 Fragments arranged by size: 7079 2450 1541 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 804 1143 1147 1638 3634 3939 4831 5272 Size: 804 339 4 491 1996 305 892 441 Cuts at: 5272 6849 7515 7854 11070 Size: 1577 666 339 3216 Fragments arranged by size: 3216 1996 1577 892 804 666 491 441 339 339 305 4 XhoI C'TCGA_G Cuts at: 0 2686 11070 Size: 2686 8384 XmaI C'CCGG_G Cuts at: 0 47 3464 3605 6402 6983 7049 7259 8177 Size: 47 3417 141 2797 581 66 210 918 Cuts at: 8177 9900 10331 11070 Size: 1723 431 739 Fragments arranged by size: 3417 2797 1723 918 739 581 431 210 141 66 47 ZraI GAC'GTC Cuts at: 0 712 3288 5556 7524 11070 Size: 712 2576 2268 1968 3546 Fragments arranged by size: 3546 2576 2268 1968 712 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI HaeII Hin4I HincII KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI Enzymes that do not cut: AclI AflII AseI AsiSI AvrII BfrBI BsiWI BstBI BstZ17I DraI EcoRI FspAI HindIII HpaI MluI NdeI NruI NsiI PacI PmeI PsiI PsrI PvuI RsrII SgfI SnaBI SpeI SspI SwaI XbaI XmnI