Restriction map for
pF1KSDA0537
(Linear) (Six-base) MAPSORT of: KIAA0537.seq Check: 2815 from: 1 to: 1983
KIAA0537.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 1946 1983
Size: 1946 37
Acc65I G'GTAC_C
Cuts at: 0 1880 1983
Size: 1880 103
AclI AA'CG_TT
Cuts at: 0 631 1983
Size: 631 1352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1222 1894 1983
Size: 1123 99 672 89
Fragments arranged by size:
1123 672 99 89
AfeI AGC'GCT
Cuts at: 0 1958 1983
Size: 1958 25
AhdI GACnn_n'nnGTC
Cuts at: 0 969 1983
Size: 969 1014
AlwNI CAG_nnn'CTG
Cuts at: 0 1156 1660 1872 1983
Size: 1156 504 212 111
Fragments arranged by size:
1156 504 212 111
ApaI G_GGCC'C
Cuts at: 0 714 1983
Size: 714 1269
ApaLI G'TGCA_C
Cuts at: 0 496 1983
Size: 496 1487
AvaI C'yCGr_G
Cuts at: 0 68 836 1983
Size: 68 768 1147
Fragments arranged by size:
1147 768 68
BamHI G'GATC_C
Cuts at: 0 1196 1983
Size: 1196 787
BanI G'GyrC_C
Cuts at: 0 54 190 1001 1347 1600 1880 1983
Size: 54 136 811 346 253 280 103
Fragments arranged by size:
811 346 280 253 136 103 54
BanII G_rGCy'C
Cuts at: 0 64 107 714 1847 1983
Size: 64 43 607 1133 136
Fragments arranged by size:
1133 607 136 64 43
BbeI G_GCGC'C
Cuts at: 0 58 1983
Size: 58 1925
BbsI GAAGACnn'nnnn_
Cuts at: 0 1451 1983
Size: 1451 532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1753 1787 1983
Size: 1753 34 196
Fragments arranged by size:
1753 196 34
BclI T'GATC_A
Cuts at: 0 386 1983
Size: 386 1597
BfrBI ATG'CAT
Cuts at: 0 655 1983
Size: 655 1328
BglII A'GATC_T
Cuts at: 0 1964 1983
Size: 1964 19
Bme1580I G_kGCm'C
Cuts at: 0 500 714 1603 1983
Size: 500 214 889 380
Fragments arranged by size:
889 500 380 214
BmrI ACTGGGnnnn_n'
Cuts at: 0 930 1860 1983
Size: 930 930 123
Fragments arranged by size:
930 930 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1245 1698 1983
Size: 1245 453 285
Fragments arranged by size:
1245 453 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1807 1831 1981 1983
Size: 121 1686 24 150 2
Fragments arranged by size:
1686 150 121 24 2
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 454 672 1983
Size: 169 285 218 1311
Fragments arranged by size:
1311 285 218 169
BsaBI GATnn'nnATC
Cuts at: 0 313 385 1983
Size: 313 72 1598
Fragments arranged by size:
1598 313 72
BsaHI Gr'CG_yC
Cuts at: 0 55 444 1983
Size: 55 389 1539
Fragments arranged by size:
1539 389 55
BsaWI w'CCGG_w
Cuts at: 0 1883 1983
Size: 1883 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1534 1564 1983
Size: 1534 30 419
Fragments arranged by size:
1534 419 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1312 1906 1983
Size: 1312 594 77
Fragments arranged by size:
1312 594 77
BseYI C'CCAG_C
Cuts at: 0 48 707 1412 1510 1524 1611 1983
Size: 48 659 705 98 14 87 372
Fragments arranged by size:
705 659 372 98 87 48 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1326 1983
Size: 1326 657
BsiEI CG_ry'CG
Cuts at: 0 876 1099 1983
Size: 876 223 884
Fragments arranged by size:
884 876 223
BsiHKAI G_wGCw'C
Cuts at: 0 500 1322 1983
Size: 500 822 661
Fragments arranged by size:
822 661 500
BsmBI CGTCTCn'nnnn_
Cuts at: 0 492 1809 1983
Size: 492 1317 174
Fragments arranged by size:
1317 492 174
Bsp1286I G_dGCh'C
Cuts at: 0 64 107 500 714 1322 1603 1847 1983
Size: 64 43 393 214 608 281 244 136
Fragments arranged by size:
608 393 281 244 214 136 64 43
BsrBI CCG'CTC
Cuts at: 0 438 1089 1983
Size: 438 651 894
Fragments arranged by size:
894 651 438
BsrDI GCAATG_nn'
Cuts at: 0 896 1983
Size: 896 1087
BsrFI r'CCGG_y
Cuts at: 0 1853 1889 1983
Size: 1853 36 94
Fragments arranged by size:
1853 94 36
BstEII G'GTnAC_C
Cuts at: 0 1533 1983
Size: 1533 450
BstYI r'GATC_y
Cuts at: 0 1196 1826 1964 1983
Size: 1196 630 138 19
Fragments arranged by size:
1196 630 138 19
BtgI C'CryG_G
Cuts at: 0 1604 1983
Size: 1604 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1210 1733 1983
Size: 1210 523 250
Fragments arranged by size:
1210 523 250
BtsI GCAGTG_nn'
Cuts at: 0 1157 1458 1983
Size: 1157 301 525
Fragments arranged by size:
1157 525 301
DraIII CAC_nnn'GTG
Cuts at: 0 123 1983
Size: 123 1860
EaeI y'GGCC_r
Cuts at: 0 231 1055 1376 1983
Size: 231 824 321 607
Fragments arranged by size:
824 607 321 231
EarI CTCTTCn'nnn_
Cuts at: 0 1443 1983
Size: 1443 540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1123 1222 1807 1831 1894 1981 1983
Size: 121 1002 99 585 24 63 87 2
Fragments arranged by size:
1002 585 121 99 87 63 24 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 686 1557 1983
Size: 686 871 426
Fragments arranged by size:
871 686 426
EcoO109I rG'GnC_Cy
Cuts at: 0 711 1050 1677 1983
Size: 711 339 627 306
Fragments arranged by size:
711 627 339 306
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 165 1587 1983
Size: 165 1422 396
Fragments arranged by size:
1422 396 165
HaeII r_GCGC'y
Cuts at: 0 58 1960 1983
Size: 58 1902 23
Fragments arranged by size:
1902 58 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 955 987 1983
Size: 304 32 619 32 996
Fragments arranged by size:
996 619 304 32 32
KasI G'GCGC_C
Cuts at: 0 54 1983
Size: 54 1929
KpnI G_GTAC'C
Cuts at: 0 1884 1983
Size: 1884 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1451 1983
Size: 623 828 532
Fragments arranged by size:
828 623 532
MscI TGG'CCA
Cuts at: 0 1057 1983
Size: 1057 926
MslI CAynn'nnrTG
Cuts at: 0 1924 1983
Size: 1924 59
MspA1I CmG'CkG
Cuts at: 0 707 800 1095 1597 1799 1983
Size: 707 93 295 502 202 184
Fragments arranged by size:
707 502 295 202 184 93
NarI GG'CG_CC
Cuts at: 0 55 1983
Size: 55 1928
NcoI C'CATG_G
Cuts at: 0 1604 1983
Size: 1604 379
NsiI A_TGCA'T
Cuts at: 0 657 1983
Size: 657 1326
PasI CC'CwG_GG
Cuts at: 0 180 714 1983
Size: 180 534 1269
Fragments arranged by size:
1269 534 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 908 1983
Size: 908 1075
PfoI T'CCnGG_A
Cuts at: 0 1684 1829 1983
Size: 1684 145 154
Fragments arranged by size:
1684 154 145
PshAI GACnn'nnGTC
Cuts at: 0 1942 1983
Size: 1942 41
PspOMI G'GGCC_C
Cuts at: 0 710 1983
Size: 710 1273
PspXI vC'TCGA_Gb
Cuts at: 0 836 1983
Size: 836 1147
PstI C_TGCA'G
Cuts at: 0 96 173 1025 1812 1972 1983
Size: 96 77 852 787 160 11
Fragments arranged by size:
852 787 160 96 77 11
PvuI CG_AT'CG
Cuts at: 0 876 1983
Size: 876 1107
PvuII CAG'CTG
Cuts at: 0 707 1799 1983
Size: 707 1092 184
Fragments arranged by size:
1092 707 184
SfcI C'TryA_G
Cuts at: 0 92 169 1021 1692 1808 1968 1983
Size: 92 77 852 671 116 160 15
Fragments arranged by size:
852 671 160 116 92 77 15
SfoI GGC'GCC
Cuts at: 0 56 1983
Size: 56 1927
SmlI C'TyrA_G
Cuts at: 0 836 1983
Size: 836 1147
SspI AAT'ATT
Cuts at: 0 573 1983
Size: 573 1410
StyI C'CwwG_G
Cuts at: 0 1190 1604 1983
Size: 1190 414 379
Fragments arranged by size:
1190 414 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 903 1983
Size: 706 197 1080
Fragments arranged by size:
1080 706 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 994 1026 1534 1566 1983
Size: 994 32 508 32 417
Fragments arranged by size:
994 508 417 32 32
XhoI C'TCGA_G
Cuts at: 0 836 1983
Size: 836 1147
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AhdI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BcgI
BclI BfrBI BglII Bme1580I BmrI BplI BpmI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstEII BstYI
BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NsiI PasI PflMI PfoI
PshAI PspOMI PspXI PstI PvuI PvuII SfcI SfoI
SmlI SspI StyI TaqII TstI XhoI
Enzymes that do not cut:
AarI AatII AflII AflIII AgeI AleI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BglI BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsiWI
BsmI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PmeI PmlI
PpiI PpuMI PsiI PsrI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI
Tth111I XbaI XcmI XmaI XmnI ZraI