Restriction map for
pF1KA0540
(Linear) (Six-base) MAPSORT of: KIAA0540.seq Check: 4347 from: 1 to: 8262
KIAA0540.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 142 1238 1613 1663 3006 3981 5939 8262
Size: 142 1096 375 50 1343 975 1958 2323
Fragments arranged by size:
2323 1958 1343 1096 975 375 142 50
AccI GT'mk_AC
Cuts at: 0 2093 5837 6926 8262
Size: 2093 3744 1089 1336
Fragments arranged by size:
3744 2093 1336 1089
Acc65I G'GTAC_C
Cuts at: 0 1773 8262
Size: 1773 6489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 2055 2747 2804 2866 3968 4628 5386
Size: 100 1955 692 57 62 1102 660 758
Cuts at: 5386 5539 7033 7281 7287 8262
Size: 153 1494 248 6 975
Fragments arranged by size:
1955 1494 1102 975 758 692 660 248
153 100 62 57 6
AfeI AGC'GCT
Cuts at: 0 4568 4978 5565 8262
Size: 4568 410 587 2697
Fragments arranged by size:
4568 2697 587 410
AflIII A'CryG_T
Cuts at: 0 3131 5183 6317 8262
Size: 3131 2052 1134 1945
Fragments arranged by size:
3131 2052 1945 1134
AhdI GACnn_n'nnGTC
Cuts at: 0 2777 5208 6318 7001 8262
Size: 2777 2431 1110 683 1261
Fragments arranged by size:
2777 2431 1261 1110 683
AleI CACnn'nnGTG
Cuts at: 0 1465 1673 4342 4453 5359 8262
Size: 1465 208 2669 111 906 2903
Fragments arranged by size:
2903 2669 1465 906 208 111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2548 3562 4995 7676 8119 8262
Size: 2548 1014 1433 2681 443 143
Fragments arranged by size:
2681 2548 1433 1014 443 143
AlwNI CAG_nnn'CTG
Cuts at: 0 78 1374 1548 1678 1801 2082 2796 2851
Size: 78 1296 174 130 123 281 714 55
Cuts at: 2851 3006 3165 3627 4629 4924 4960 4969 5304
Size: 155 159 462 1002 295 36 9 335
Cuts at: 5304 6798 7011 7104 7344 7644 8262
Size: 1494 213 93 240 300 618
Fragments arranged by size:
1494 1296 1002 714 618 462 335 300
295 281 240 213 174 159 155 130
123 93 78 55 36 9
ApaI G_GGCC'C
Cuts at: 0 2477 2946 3041 7875 8262
Size: 2477 469 95 4834 387
Fragments arranged by size:
4834 2477 469 387 95
ApaLI G'TGCA_C
Cuts at: 0 1968 2463 8262
Size: 1968 495 5799
Fragments arranged by size:
5799 1968 495
ApoI r'AATT_y
Cuts at: 0 535 6652 8262
Size: 535 6117 1610
Fragments arranged by size:
6117 1610 535
AscI GG'CGCG_CC
Cuts at: 0 5332 8262
Size: 5332 2930
AvaI C'yCGr_G
Cuts at: 0 314 1046 1057 5662 7562 8226 8262
Size: 314 732 11 4605 1900 664 36
Fragments arranged by size:
4605 1900 732 664 314 36 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3727 3760 8262
Size: 3727 33 4502
Fragments arranged by size:
4502 3727 33
BanI G'GyrC_C
Cuts at: 0 94 458 1050 1709 1773 2060 2359 2487
Size: 94 364 592 659 64 287 299 128
Cuts at: 2487 2609 2677 4992 5129 5261 5420 5526 5900
Size: 122 68 2315 137 132 159 106 374
Cuts at: 5900 6469 7252 7362 7623 7783 7987 8262
Size: 569 783 110 261 160 204 275
Fragments arranged by size:
2315 783 659 592 569 374 364 299
287 275 261 204 160 159 137 132
128 122 110 106 94 68 64
BanII G_rGCy'C
Cuts at: 0 880 1100 2477 2522 2946 3041 4262 5969
Size: 880 220 1377 45 424 95 1221 1707
Cuts at: 5969 6524 6625 6644 7852 7875 8262
Size: 555 101 19 1208 23 387
Fragments arranged by size:
1707 1377 1221 1208 880 555 424 387
220 101 95 45 23 19
BbeI G_GCGC'C
Cuts at: 0 462 1054 2064 2613 5133 5265 5424 5904
Size: 462 592 1010 549 2520 132 159 480
Cuts at: 5904 8262
Size: 2358
Fragments arranged by size:
2520 2358 1010 592 549 480 462 159
132
BbsI GAAGACnn'nnnn_
Cuts at: 0 959 3905 3984 4778 5102 6345 6888 8262
Size: 959 2946 79 794 324 1243 543 1374
Fragments arranged by size:
2946 1374 1243 959 794 543 324 79
BbvCI CC'TCA_GC
Cuts at: 0 982 1649 3026 3760 4443 5455 6337 7043
Size: 982 667 1377 734 683 1012 882 706
Cuts at: 7043 8262
Size: 1219
Fragments arranged by size:
1377 1219 1012 982 882 734 706 683
667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4372 4406 8115 8149 8160 8194 8262
Size: 4372 34 3709 34 11 34 68
Fragments arranged by size:
4372 3709 68 34 34 34 11
BciVI GTATCCnnnnn_n'
Cuts at: 0 1836 2176 8262
Size: 1836 340 6086
Fragments arranged by size:
6086 1836 340
BclI T'GATC_A
Cuts at: 0 7730 8262
Size: 7730 532
BglI GCCn_nnn'nGGC
Cuts at: 0 138 1484 1525 2239 2440 2618 3841 4157
Size: 138 1346 41 714 201 178 1223 316
Cuts at: 4157 5037 6607 6883 8262
Size: 880 1570 276 1379
Fragments arranged by size:
1570 1379 1346 1223 880 714 316 276
201 178 138 41
BglII A'GATC_T
Cuts at: 0 3720 8262
Size: 3720 4542
BlpI GC'TnA_GC
Cuts at: 0 1394 2521 3349 3977 5705 7492 7851 8262
Size: 1394 1127 828 628 1728 1787 359 411
Fragments arranged by size:
1787 1728 1394 1127 828 628 411 359
Bme1580I G_kGCm'C
Cuts at: 0 314 825 1324 1972 2467 2477 2682 2946
Size: 314 511 499 648 495 10 205 264
Cuts at: 2946 3041 5531 6022 7255 7875 7990 8262
Size: 95 2490 491 1233 620 115 272
Fragments arranged by size:
2490 1233 648 620 511 499 495 491
314 272 264 205 115 95 10
BmgBI CAC'GTC
Cuts at: 0 3327 4361 6540 8262
Size: 3327 1034 2179 1722
Fragments arranged by size:
3327 2179 1722 1034
BmrI ACTGGGnnnn_n'
Cuts at: 0 1576 2265 2585 4894 5163 5415 6022 7446
Size: 1576 689 320 2309 269 252 607 1424
Cuts at: 7446 7989 8262
Size: 543 273
Fragments arranged by size:
2309 1576 1424 689 607 543 320 273
269 252
BmtI G_CTAG'C
Cuts at: 0 1519 7165 8262
Size: 1519 5646 1097
Fragments arranged by size:
5646 1519 1097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2172 4998 5480 5631 8262
Size: 2172 2826 482 151 2631
Fragments arranged by size:
2826 2631 2172 482 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 244 295 653 726 1543 1861 2416
Size: 148 96 51 358 73 817 318 555
Cuts at: 2416 2716 3604 3733 3901 4127 4203 4258 4510
Size: 300 888 129 168 226 76 55 252
Cuts at: 4510 4522 4894 5359 5731 5836 6637 6694 6697
Size: 12 372 465 372 105 801 57 3
Cuts at: 6697 6820 6988 8137 8262
Size: 123 168 1149 125
Fragments arranged by size:
1149 888 817 801 555 465 372 372
358 318 300 252 226 168 168 148
129 125 123 105 96 76 73 57
55 51 12 3
Bpu10I CC'TnA_GC
Cuts at: 0 879 982 1649 1917 3026 3578 3760 4443
Size: 879 103 667 268 1109 552 182 683
Cuts at: 4443 5455 6148 6337 7043 8262
Size: 1012 693 189 706 1219
Fragments arranged by size:
1219 1109 1012 879 706 693 683 667
552 268 189 182 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 850 5983 6122 7099 8262
Size: 229 621 5133 139 977 1163
Fragments arranged by size:
5133 1163 977 621 229 139
BsaI GGTCTCn'nnnn_
Cuts at: 0 2626 3622 5707 6048 8262
Size: 2626 996 2085 341 2214
Fragments arranged by size:
2626 2214 2085 996 341
BsaAI yAC'GTr
Cuts at: 0 1726 4800 5186 6318 7574 8103 8262
Size: 1726 3074 386 1132 1256 529 159
Fragments arranged by size:
3074 1726 1256 1132 529 386 159
BsaHI Gr'CG_yC
Cuts at: 0 459 926 1051 1133 2061 2610 3182 5130
Size: 459 467 125 82 928 549 572 1948
Cuts at: 5130 5262 5421 5901 8262
Size: 132 159 480 2361
Fragments arranged by size:
2361 1948 928 572 549 480 467 459
159 132 125 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 4125 4155 6793 6799 6823 6829
Size: 4 30 4091 30 2638 6 24 6
Cuts at: 6829 8262
Size: 1433
Fragments arranged by size:
4091 2638 1433 30 30 24 6 6
4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 791 2148 2527 4861 5623 5890 6895
Size: 160 631 1357 379 2334 762 267 1005
Cuts at: 6895 8262
Size: 1367
Fragments arranged by size:
2334 1367 1357 1005 762 631 379 267
160
BseYI C'CCAG_C
Cuts at: 0 1753 2280 2300 2406 2966 2999 3662 4430
Size: 1753 527 20 106 560 33 663 768
Cuts at: 4430 4434 4559 5034 5912 6059 6345 6401 6438
Size: 4 125 475 878 147 286 56 37
Cuts at: 6438 7248 7983 8262
Size: 810 735 279
Fragments arranged by size:
1753 878 810 768 735 663 560 527
475 286 279 147 125 106 56 37
33 20 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 193 788 1489 1706 1816 2693 2935
Size: 176 17 595 701 217 110 877 242
Cuts at: 2935 3286 3349 3403 3454 4041 4865 5386 6520
Size: 351 63 54 51 587 824 521 1134
Cuts at: 6520 6803 6891 7445 7657 7990 8262
Size: 283 88 554 212 333 272
Fragments arranged by size:
1134 877 824 701 595 587 554 521
351 333 283 272 242 217 212 176
110 88 63 54 51 17
BsiEI CG_ry'CG
Cuts at: 0 1946 3531 5771 6576 8039 8262
Size: 1946 1585 2240 805 1463 223
Fragments arranged by size:
2240 1946 1585 1463 805 223
BsiHKAI G_wGCw'C
Cuts at: 0 880 1731 1972 2467 2522 2990 3155 4427
Size: 880 851 241 495 55 468 165 1272
Cuts at: 4427 4865 5656 5969 6644 7852 8262
Size: 438 791 313 675 1208 410
Fragments arranged by size:
1272 1208 880 851 791 675 495 468
438 410 313 241 165 55
BsmI GAATG_Cn'
Cuts at: 0 573 5229 7120 8262
Size: 573 4656 1891 1142
Fragments arranged by size:
4656 1891 1142 573
BsmBI CGTCTCn'nnnn_
Cuts at: 0 607 2476 8227 8262
Size: 607 1869 5751 35
Fragments arranged by size:
5751 1869 607 35
Bsp1286I G_dGCh'C
Cuts at: 0 314 825 880 1100 1324 1731 1972 2467
Size: 314 511 55 220 224 407 241 495
Cuts at: 2467 2477 2522 2682 2946 2990 3041 3155 4262
Size: 10 45 160 264 44 51 114 1107
Cuts at: 4262 4427 4865 5531 5656 5969 6022 6524 6625
Size: 165 438 666 125 313 53 502 101
Cuts at: 6625 6644 7255 7852 7875 7990 8262
Size: 19 611 597 23 115 272
Fragments arranged by size:
1107 666 611 597 511 502 495 438
407 314 313 272 264 241 224 220
165 160 125 115 114 101 55 53
51 45 44 23 19 10
BspHI T'CATG_A
Cuts at: 0 1172 8262
Size: 1172 7090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 142 1046 1174 1238 1613 1663 2722
Size: 74 68 904 128 64 375 50 1059
Cuts at: 2722 3006 3321 3410 3981 5325 5939 5954 7525
Size: 284 315 89 571 1344 614 15 1571
Cuts at: 7525 7629 8262
Size: 104 633
Fragments arranged by size:
1571 1344 1059 904 633 614 571 375
315 284 128 104 89 74 68 64
50 15
BsrBI CCG'CTC
Cuts at: 0 15 2267 3570 3582 6158 6951 8262
Size: 15 2252 1303 12 2576 793 1311
Fragments arranged by size:
2576 2252 1311 1303 793 15 12
BsrDI GCAATG_nn'
Cuts at: 0 1136 1381 6251 8262
Size: 1136 245 4870 2011
Fragments arranged by size:
4870 2011 1136 245
BsrFI r'CCGG_y
Cuts at: 0 956 1350 2063 3895 6575 7178 8262
Size: 956 394 713 1832 2680 603 1084
Fragments arranged by size:
2680 1832 1084 956 713 603 394
BsrGI T'GTAC_A
Cuts at: 0 1782 1892 8262
Size: 1782 110 6370
Fragments arranged by size:
6370 1782 110
BssHII G'CGCG_C
Cuts at: 0 5274 5332 8257 8262
Size: 5274 58 2925 5
Fragments arranged by size:
5274 2925 58 5
BssSI C'ACGA_G
Cuts at: 0 803 2755 6108 6406 6829 8262
Size: 803 1952 3353 298 423 1433
Fragments arranged by size:
3353 1952 1433 803 423 298
BstAPI GCAn_nnn'nTGC
Cuts at: 0 992 1284 1374 2851 3345 3858 4815 4960
Size: 992 292 90 1477 494 513 957 145
Cuts at: 4960 4969 7344 8262
Size: 9 2375 918
Fragments arranged by size:
2375 1477 992 957 918 513 494 292
145 90 9
BstBI TT'CG_AA
Cuts at: 0 5174 8262
Size: 5174 3088
BstEII G'GTnAC_C
Cuts at: 0 61 1306 4618 5820 7878 8262
Size: 61 1245 3312 1202 2058 384
Fragments arranged by size:
3312 2058 1245 1202 384 61
BstXI CCAn_nnnn'nTGG
Cuts at: 0 342 469 942 2056 2394 4236 6381 7441
Size: 342 127 473 1114 338 1842 2145 1060
Cuts at: 7441 7578 8061 8262
Size: 137 483 201
Fragments arranged by size:
2145 1842 1114 1060 483 473 342 338
201 137 127
BstYI r'GATC_y
Cuts at: 0 3542 3720 7711 8262
Size: 3542 178 3991 551
Fragments arranged by size:
3991 3542 551 178
BstZ17I GTA'TAC
Cuts at: 0 5838 8262
Size: 5838 2424
Bsu36I CC'TnA_GG
Cuts at: 0 1423 2501 2552 3644 6183 6767 8262
Size: 1423 1078 51 1092 2539 584 1495
Fragments arranged by size:
2539 1495 1423 1092 1078 584 51
BtgI C'CryG_G
Cuts at: 0 711 1251 1589 2972 4183 4535 4614 5390
Size: 711 540 338 1383 1211 352 79 776
Cuts at: 5390 5798 6751 7319 7357 7470 7749 8262
Size: 408 953 568 38 113 279 513
Fragments arranged by size:
1383 1211 953 776 711 568 540 513
408 352 338 279 113 79 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1803 3386 4397 6749 7042 8262
Size: 1803 1583 1011 2352 293 1220
Fragments arranged by size:
2352 1803 1583 1220 1011 293
BtsI GCAGTG_nn'
Cuts at: 0 79 474 1375 1552 2031 2852 2923 3511
Size: 79 395 901 177 479 821 71 588
Cuts at: 3511 4106 4134 4835 5050 5256 5366 5617 5636
Size: 595 28 701 215 206 110 251 19
Cuts at: 5636 7377 7403 7670 7927 8026 8262
Size: 1741 26 267 257 99 236
Fragments arranged by size:
1741 901 821 701 595 588 479 395
267 257 251 236 215 206 177 110
99 79 71 28 26 19
ClaI AT'CG_AT
Cuts at: 0 5978 8262
Size: 5978 2284
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2277 2312 5073 5108 5648 5683 8262
Size: 2277 35 2761 35 540 35 2579
Fragments arranged by size:
2761 2579 2277 540 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2037 2375 2628 2895 6591 7574 8262
Size: 2037 338 253 267 3696 983 688
Fragments arranged by size:
3696 2037 983 688 338 267 253
DrdI GACnn_nn'nnGTC
Cuts at: 0 3331 8262
Size: 3331 4931
EaeI y'GGCC_r
Cuts at: 0 2 197 862 958 1190 1618 1703 1792
Size: 2 195 665 96 232 428 85 89
Cuts at: 1792 1943 2103 2417 3362 3502 4206 4407 4547
Size: 151 160 314 945 140 704 201 140
Cuts at: 4547 4697 5768 5795 6555 7431 7676 8036 8063
Size: 150 1071 27 760 876 245 360 27
Cuts at: 8063 8078 8262
Size: 15 184
Fragments arranged by size:
1071 945 876 760 704 665 428 360
314 245 232 201 195 184 160 151
150 140 140 96 89 85 27 27
15 2
EagI C'GGCC_G
Cuts at: 0 1943 5768 8036 8262
Size: 1943 3825 2268 226
Fragments arranged by size:
3825 2268 1943 226
EarI CTCTTCn'nnn_
Cuts at: 0 100 256 490 619 884 1411 3076 4313
Size: 100 156 234 129 265 527 1665 1237
Cuts at: 4313 4911 5974 8262
Size: 598 1063 2288
Fragments arranged by size:
2288 1665 1237 1063 598 527 265 234
156 129 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 361 1658 2985 6276 8262
Size: 361 1297 1327 3291 1986
Fragments arranged by size:
3291 1986 1327 1297 361
EcoICRI GAG'CTC
Cuts at: 0 878 2520 5967 6642 7850 8262
Size: 878 1642 3447 675 1208 412
Fragments arranged by size:
3447 1642 1208 878 675 412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 148 244 295 653 726 1543 1861
Size: 100 48 96 51 358 73 817 318
Cuts at: 1861 2055 2416 2716 2747 2804 2866 3604 3733
Size: 194 361 300 31 57 62 738 129
Cuts at: 3733 3901 3968 4127 4203 4258 4510 4522 4628
Size: 168 67 159 76 55 252 12 106
Cuts at: 4628 4894 5359 5386 5539 5731 5836 6637 6694
Size: 266 465 27 153 192 105 801 57
Cuts at: 6694 6697 6820 6988 7033 7281 7287 8137 8262
Size: 3 123 168 45 248 6 850 125
Fragments arranged by size:
850 817 801 738 465 361 358 318
300 266 252 248 194 192 168 168
159 153 129 125 123 106 105 100
96 76 73 67 62 57 57 55
51 48 45 31 27 12 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1234 2329 2953 3433 7597 8262
Size: 1234 1095 624 480 4164 665
Fragments arranged by size:
4164 1234 1095 665 624 480
EcoO109I rG'GnC_Cy
Cuts at: 0 54 162 429 681 883 894 1113 1717
Size: 54 108 267 252 202 11 219 604
Cuts at: 1717 1798 1949 2456 2473 2761 2793 2926 2942
Size: 81 151 507 17 288 32 133 16
Cuts at: 2942 2943 2958 3038 3675 4008 4439 4530 4542
Size: 1 15 80 637 333 431 91 12
Cuts at: 4542 4937 5444 6303 6360 6435 6885 7641 7871
Size: 395 507 859 57 75 450 756 230
Cuts at: 7871 8262
Size: 391
Fragments arranged by size:
859 756 637 604 507 507 450 431
395 391 333 288 267 252 230 219
202 151 133 108 91 81 80 75
57 54 32 17 16 15 12 11
1
EcoRI G'AATT_C
Cuts at: 0 535 6652 8262
Size: 535 6117 1610
Fragments arranged by size:
6117 1610 535
EcoRV GAT'ATC
Cuts at: 0 6218 8262
Size: 6218 2044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 913 7140 8262
Size: 913 6227 1122
Fragments arranged by size:
6227 1122 913
FspI TGC'GCA
Cuts at: 0 3208 3550 5005 5321 8167 8262
Size: 3208 342 1455 316 2846 95
Fragments arranged by size:
3208 2846 1455 342 316 95
FspAI rTGC'GCAy
Cuts at: 0 5005 8262
Size: 5005 3257
HaeII r_GCGC'y
Cuts at: 0 462 545 1054 2064 2613 3237 3597 4570
Size: 462 83 509 1010 549 624 360 973
Cuts at: 4570 4980 5133 5265 5416 5424 5455 5495 5567
Size: 410 153 132 151 8 31 40 72
Cuts at: 5567 5904 8262
Size: 337 2358
Fragments arranged by size:
2358 1010 973 624 549 509 462 410
360 337 153 151 132 83 72 40
31 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1171 1203 1239 1271 2763 2795 4125 4157
Size: 1171 32 36 32 1492 32 1330 32
Cuts at: 4157 4267 4299 4921 4953 5194 5226 6304 6336
Size: 110 32 622 32 241 32 1078 32
Cuts at: 6336 6799 6831 6987 7019 7066 7098 8262
Size: 463 32 156 32 47 32 1164
Fragments arranged by size:
1492 1330 1171 1164 1078 622 463 241
156 110 47 36 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 332 8262
Size: 332 7930
HindIII A'AGCT_T
Cuts at: 0 6426 8262
Size: 6426 1836
KasI G'GCGC_C
Cuts at: 0 458 1050 2060 2609 5129 5261 5420 5900
Size: 458 592 1010 549 2520 132 159 480
Cuts at: 5900 8262
Size: 2362
Fragments arranged by size:
2520 2362 1010 592 549 480 458 159
132
KpnI G_GTAC'C
Cuts at: 0 1777 8262
Size: 1777 6485
MfeI C'AATT_G
Cuts at: 0 990 8262
Size: 990 7272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 1475 3440 6245 6596 6785 7051 8040
Size: 371 1104 1965 2805 351 189 266 989
Cuts at: 8040 8262
Size: 222
Fragments arranged by size:
2805 1965 1104 989 371 351 266 222
189
MscI TGG'CCA
Cuts at: 0 864 1705 1794 2419 4409 4549 4699 7433
Size: 864 841 89 625 1990 140 150 2734
Cuts at: 7433 7678 8065 8262
Size: 245 387 197
Fragments arranged by size:
2734 1990 864 841 625 387 245 197
150 140 89
MslI CAynn'nnrTG
Cuts at: 0 934 1297 1465 1673 2977 2983 4342 4453
Size: 934 363 168 208 1304 6 1359 111
Cuts at: 4453 5124 5242 5359 6379 6514 7439 7505 7576
Size: 671 118 117 1020 135 925 66 71
Cuts at: 7576 7601 7702 8059 8137 8262
Size: 25 101 357 78 125
Fragments arranged by size:
1359 1304 1020 934 925 671 363 357
208 168 135 125 118 117 111 101
78 71 66 25 6
MspA1I CmG'CkG
Cuts at: 0 168 207 868 1393 1442 1532 1545 1890
Size: 168 39 661 525 49 90 13 345
Cuts at: 1890 1913 2179 2740 3003 3025 3035 3228 3741
Size: 23 266 561 263 22 10 193 513
Cuts at: 3741 4185 4434 4711 4782 4951 4960 4969 5034
Size: 444 249 277 71 169 9 9 65
Cuts at: 5034 5259 5477 5781 5916 6546 6714 6864 7008
Size: 225 218 304 135 630 168 150 144
Cuts at: 7008 7042 7262 7427 7496 7751 8262
Size: 34 220 165 69 255 511
Fragments arranged by size:
661 630 561 525 513 511 444 345
304 277 266 263 255 249 225 220
218 193 169 168 168 165 150 144
135 90 71 69 65 49 39 34
23 22 13 10 9 9
NaeI GCC'GGC
Cuts at: 0 2065 3897 8262
Size: 2065 1832 4365
Fragments arranged by size:
4365 2065 1832
NarI GG'CG_CC
Cuts at: 0 459 1051 2061 2610 5130 5262 5421 5901
Size: 459 592 1010 549 2520 132 159 480
Cuts at: 5901 8262
Size: 2361
Fragments arranged by size:
2520 2361 1010 592 549 480 459 159
132
NcoI C'CATG_G
Cuts at: 0 1251 1589 2972 4614 5390 7319 8262
Size: 1251 338 1383 1642 776 1929 943
Fragments arranged by size:
1929 1642 1383 1251 943 776 338
NgoMIV G'CCGG_C
Cuts at: 0 2063 3895 8262
Size: 2063 1832 4367
Fragments arranged by size:
4367 2063 1832
NheI G'CTAG_C
Cuts at: 0 1515 7161 8262
Size: 1515 5646 1101
Fragments arranged by size:
5646 1515 1101
NspI r_CATG'y
Cuts at: 0 193 412 787 921 950 1320 3135 4789
Size: 193 219 375 134 29 370 1815 1654
Cuts at: 4789 4815 5241 5514 6400 8262
Size: 26 426 273 886 1862
Fragments arranged by size:
1862 1815 1654 886 426 375 370 273
219 193 134 29 26
PasI CC'CwG_GG
Cuts at: 0 372 2469 2790 5806 6299 7203 8262
Size: 372 2097 321 3016 493 904 1059
Fragments arranged by size:
3016 2097 1059 904 493 372 321
PciI A'CATG_T
Cuts at: 0 3131 8262
Size: 3131 5131
PflMI CCAn_nnn'nTGG
Cuts at: 0 1801 2082 2628 2796 3627 4177 5304 6591
Size: 1801 281 546 168 831 550 1127 1287
Cuts at: 6591 6798 7104 7372 7839 8106 8262
Size: 207 306 268 467 267 156
Fragments arranged by size:
1801 1287 1127 831 550 546 467 306
281 268 267 207 168 156
PfoI T'CCnGG_A
Cuts at: 0 1115 3878 4253 4487 5297 6356 6647 6674
Size: 1115 2763 375 234 810 1059 291 27
Cuts at: 6674 8262
Size: 1588
Fragments arranged by size:
2763 1588 1115 1059 810 375 291 234
27
PmlI CAC'GTG
Cuts at: 0 1726 7574 8103 8262
Size: 1726 5848 529 159
Fragments arranged by size:
5848 1726 529 159
PpuMI rG'GwC_Cy
Cuts at: 0 54 162 1113 1717 1949 2456 2761 2958
Size: 54 108 951 604 232 507 305 197
Cuts at: 2958 4542 4937 6303 6360 6435 7641 8262
Size: 1584 395 1366 57 75 1206 621
Fragments arranged by size:
1584 1366 1206 951 621 604 507 395
305 232 197 108 75 57 54
PshAI GACnn'nnGTC
Cuts at: 0 3779 6028 8262
Size: 3779 2249 2234
Fragments arranged by size:
3779 2249 2234
PspOMI G'GGCC_C
Cuts at: 0 2473 2942 3037 7871 8262
Size: 2473 469 95 4834 391
Fragments arranged by size:
4834 2473 469 391 95
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6088 6120 6277 6309 8262
Size: 6088 32 157 32 1953
Fragments arranged by size:
6088 1953 157 32 32
PstI C_TGCA'G
Cuts at: 0 71 268 1043 1235 1247 2330 2441 3560
Size: 71 197 775 192 12 1083 111 1119
Cuts at: 3560 4592 4830 4956 4965 4974 5045 5675 6311
Size: 1032 238 126 9 9 71 630 636
Cuts at: 6311 7665 8262
Size: 1354 597
Fragments arranged by size:
1354 1119 1083 1032 775 636 630 597
238 197 192 126 111 71 71 12
9 9
PvuI CG_AT'CG
Cuts at: 0 3531 8262
Size: 3531 4731
PvuII CAG'CTG
Cuts at: 0 207 868 1442 1545 1890 2740 3003 3025
Size: 207 661 574 103 345 850 263 22
Cuts at: 3025 3741 4434 4711 4782 4951 4960 4969 5781
Size: 716 693 277 71 169 9 9 812
Cuts at: 5781 5916 6546 6714 7008 7042 7262 7496 8262
Size: 135 630 168 294 34 220 234 766
Fragments arranged by size:
850 812 766 716 693 661 630 574
345 294 277 263 234 220 207 169
168 135 103 71 34 22 9 9
SacI G_AGCT'C
Cuts at: 0 880 2522 5969 6644 7852 8262
Size: 880 1642 3447 675 1208 410
Fragments arranged by size:
3447 1642 1208 880 675 410
SacII CC_GC'GG
Cuts at: 0 4186 7752 8262
Size: 4186 3566 510
Fragments arranged by size:
4186 3566 510
SanDI GG'GwC_CC
Cuts at: 0 1949 4937 8262
Size: 1949 2988 3325
Fragments arranged by size:
3325 2988 1949
SapI GCTCTTCn'nnn_
Cuts at: 0 100 256 490 1411 3076 5974 8262
Size: 100 156 234 921 1665 2898 2288
Fragments arranged by size:
2898 2288 1665 921 234 156 100
SbfI CC_TGCA'GG
Cuts at: 0 1235 2330 2441 6311 8262
Size: 1235 1095 111 3870 1951
Fragments arranged by size:
3870 1951 1235 1095 111
ScaI AGT'ACT
Cuts at: 0 6232 8262
Size: 6232 2030
SexAI A'CCwGG_T
Cuts at: 0 2081 6098 8262
Size: 2081 4017 2164
Fragments arranged by size:
4017 2164 2081
SfcI C'TryA_G
Cuts at: 0 67 264 562 1039 1231 1243 2326 2437
Size: 67 197 298 477 192 12 1083 111
Cuts at: 2437 3216 3556 4588 4819 4826 4952 4961 4970
Size: 779 340 1032 231 7 126 9 9
Cuts at: 4970 5041 5671 6307 6923 7661 7949 8262
Size: 71 630 636 616 738 288 313
Fragments arranged by size:
1083 1032 779 738 636 630 616 477
340 313 298 288 231 197 192 126
111 71 67 12 9 9 7
SfoI GGC'GCC
Cuts at: 0 460 1052 2062 2611 5131 5263 5422 5902
Size: 460 592 1010 549 2520 132 159 480
Cuts at: 5902 8262
Size: 2360
Fragments arranged by size:
2520 2360 1010 592 549 480 460 159
132
SmaI CCC'GGG
Cuts at: 0 1048 7564 8262
Size: 1048 6516 698
Fragments arranged by size:
6516 1048 698
SmlI C'TyrA_G
Cuts at: 0 244 829 5962 6137 7114 8262
Size: 244 585 5133 175 977 1148
Fragments arranged by size:
5133 1148 977 585 244 175
SphI G_CATG'C
Cuts at: 0 787 921 4789 5514 6400 8262
Size: 787 134 3868 725 886 1862
Fragments arranged by size:
3868 1862 886 787 725 134
StuI AGG'CCT
Cuts at: 0 85 1478 1930 4795 4852 5614 6188 7619
Size: 85 1393 452 2865 57 762 574 1431
Cuts at: 7619 8262
Size: 643
Fragments arranged by size:
2865 1431 1393 762 643 574 452 85
57
StyI C'CwwG_G
Cuts at: 0 488 1193 1251 1589 2972 3443 4614 5390
Size: 488 705 58 338 1383 471 1171 776
Cuts at: 5390 7319 8087 8262
Size: 1929 768 175
Fragments arranged by size:
1929 1383 1171 776 768 705 488 471
338 175 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1279 2377 2665 2803 3106 3226 3379 3966
Size: 1279 1098 288 138 303 120 153 587
Cuts at: 3966 3975 4444 4531 4573 5082 5572 5616 6487
Size: 9 469 87 42 509 490 44 871
Cuts at: 6487 7216 7270 7350 7771 8262
Size: 729 54 80 421 491
Fragments arranged by size:
1279 1098 871 729 587 509 491 490
469 421 303 288 153 138 120 87
80 54 44 42 9
TatI w'GTAC_w
Cuts at: 0 1782 1892 3128 4749 6104 6230 8262
Size: 1782 110 1236 1621 1355 126 2032
Fragments arranged by size:
2032 1782 1621 1355 1236 126 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2210 2242 2359 2391 3081 3113 4309 4341
Size: 2210 32 117 32 690 32 1196 32
Cuts at: 4341 4585 4617 7558 7590 8262
Size: 244 32 2941 32 672
Fragments arranged by size:
2941 2210 1196 690 672 244 117 32
32 32 32 32 32
XbaI T'CTAG_A
Cuts at: 0 750 8262
Size: 750 7512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5444 6550 7245 8073 8262
Size: 5444 1106 695 828 189
Fragments arranged by size:
5444 1106 828 695 189
XmaI C'CCGG_G
Cuts at: 0 1046 7562 8262
Size: 1046 6516 700
Fragments arranged by size:
6516 1046 700
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AscI AvaI BaeI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmlI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AloI AseI AsiSI AvrII
BamHI BfrBI BsaBI BsaWI BsiWI BspEI DraI FseI
HpaI MluI NdeI NotI NruI NsiI PacI PmeI
PpiI PsiI PspXI RsrII SalI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XhoI XmnI
ZraI