Restriction map for
pF1KA0546
(Linear) (Six-base) MAPSORT of: KIAA0546.seq Check: 7031 from: 1 to: 5730
KIAA0546.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1164 5730
Size: 1164 4566
AccI GT'mk_AC
Cuts at: 0 3925 5730
Size: 3925 1805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1400 1490 1838 2333 2416 2612 2659
Size: 1120 280 90 348 495 83 196 47
Cuts at: 2659 3098 3701 3888 5730
Size: 439 603 187 1842
Fragments arranged by size:
1842 1120 603 495 439 348 280 196
187 90 83 47
AflII C'TTAA_G
Cuts at: 0 3172 5730
Size: 3172 2558
AflIII A'CryG_T
Cuts at: 0 272 3577 4721 5730
Size: 272 3305 1144 1009
Fragments arranged by size:
3305 1144 1009 272
AhdI GACnn_n'nnGTC
Cuts at: 0 3654 5730
Size: 3654 2076
AlwNI CAG_nnn'CTG
Cuts at: 0 246 1513 4914 5461 5667 5730
Size: 246 1267 3401 547 206 63
Fragments arranged by size:
3401 1267 547 246 206 63
ApaLI G'TGCA_C
Cuts at: 0 4487 5730
Size: 4487 1243
ApoI r'AATT_y
Cuts at: 0 252 1035 1421 2544 2639 2684 2936 3016
Size: 252 783 386 1123 95 45 252 80
Cuts at: 3016 3101 4205 4600 4621 5032 5083 5199 5413
Size: 85 1104 395 21 411 51 116 214
Cuts at: 5413 5419 5512 5730
Size: 6 93 218
Fragments arranged by size:
1123 1104 783 411 395 386 252 252
218 214 116 95 93 85 80 51
45 21 6
AvaI C'yCGr_G
Cuts at: 0 182 2753 5730
Size: 182 2571 2977
Fragments arranged by size:
2977 2571 182
AvrII C'CTAG_G
Cuts at: 0 2041 5730
Size: 2041 3689
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3977 4010 5730
Size: 3977 33 1720
Fragments arranged by size:
3977 1720 33
BamHI G'GATC_C
Cuts at: 0 541 5392 5730
Size: 541 4851 338
Fragments arranged by size:
4851 541 338
BanI G'GyrC_C
Cuts at: 0 4211 5730
Size: 4211 1519
BanII G_rGCy'C
Cuts at: 0 367 578 598 3514 5730
Size: 367 211 20 2916 2216
Fragments arranged by size:
2916 2216 367 211 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 2071 2404 5730
Size: 2071 333 3326
Fragments arranged by size:
3326 2071 333
BbvCI CC'TCA_GC
Cuts at: 0 247 5730
Size: 247 5483
BciVI GTATCCnnnnn_n'
Cuts at: 0 1593 2039 3978 5730
Size: 1593 446 1939 1752
Fragments arranged by size:
1939 1752 1593 446
BfrBI ATG'CAT
Cuts at: 0 722 3203 4510 5730
Size: 722 2481 1307 1220
Fragments arranged by size:
2481 1307 1220 722
BglII A'GATC_T
Cuts at: 0 1428 3133 5730
Size: 1428 1705 2597
Fragments arranged by size:
2597 1705 1428
BlpI GC'TnA_GC
Cuts at: 0 2234 5730
Size: 2234 3496
Bme1580I G_kGCm'C
Cuts at: 0 2964 4491 5730
Size: 2964 1527 1239
Fragments arranged by size:
2964 1527 1239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 489 1607 1667 1952 2831 2978 3983
Size: 15 474 1118 60 285 879 147 1005
Cuts at: 3983 4277 4864 5730
Size: 294 587 866
Fragments arranged by size:
1118 1005 879 866 587 474 294 285
147 60 15
Bpu10I CC'TnA_GC
Cuts at: 0 247 3508 5730
Size: 247 3261 2222
Fragments arranged by size:
3261 2222 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4078 5730
Size: 4078 1652
BsaI GGTCTCn'nnnn_
Cuts at: 0 338 620 5730
Size: 338 282 5110
Fragments arranged by size:
5110 338 282
BsaAI yAC'GTr
Cuts at: 0 4356 5730
Size: 4356 1374
BsaWI w'CCGG_w
Cuts at: 0 329 2298 3540 5730
Size: 329 1969 1242 2190
Fragments arranged by size:
2190 1969 1242 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1605 1635 1793 1823 5730
Size: 1605 30 158 30 3907
Fragments arranged by size:
3907 1605 158 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 161 497 512 545 548 877 1500 1571
Size: 161 336 15 33 3 329 623 71
Cuts at: 1571 1778 1879 3818 5730
Size: 207 101 1939 1912
Fragments arranged by size:
1939 1912 623 336 329 207 161 101
71 33 15 3
BseYI C'CCAG_C
Cuts at: 0 301 2415 5561 5730
Size: 301 2114 3146 169
Fragments arranged by size:
3146 2114 301 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 790 3704 4279 4444 4816 5730
Size: 534 256 2914 575 165 372 914
Fragments arranged by size:
2914 914 575 534 372 256 165
BsiEI CG_ry'CG
Cuts at: 0 8 391 5730
Size: 8 383 5339
Fragments arranged by size:
5339 383 8
BsiHKAI G_wGCw'C
Cuts at: 0 367 598 3514 4138 4491 5730
Size: 367 231 2916 624 353 1239
Fragments arranged by size:
2916 1239 624 367 353 231
BsmI GAATG_Cn'
Cuts at: 0 724 2868 3205 4512 5010 5565 5730
Size: 724 2144 337 1307 498 555 165
Fragments arranged by size:
2144 1307 724 555 498 337 165
Bsp1286I G_dGCh'C
Cuts at: 0 367 578 598 2964 3514 4138 4491 5730
Size: 367 211 20 2366 550 624 353 1239
Fragments arranged by size:
2366 1239 624 550 367 353 211 20
BspHI T'CATG_A
Cuts at: 0 1463 3042 5730
Size: 1463 1579 2688
Fragments arranged by size:
2688 1579 1463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1164 2463 5730
Size: 1164 1299 3267
Fragments arranged by size:
3267 1299 1164
BsrBI CCG'CTC
Cuts at: 0 282 405 5730
Size: 282 123 5325
Fragments arranged by size:
5325 282 123
BsrDI GCAATG_nn'
Cuts at: 0 2465 4868 5661 5730
Size: 2465 2403 793 69
Fragments arranged by size:
2465 2403 793 69
BsrGI T'GTAC_A
Cuts at: 0 1218 4080 5730
Size: 1218 2862 1650
Fragments arranged by size:
2862 1650 1218
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3875 4208 5577 5730
Size: 3875 333 1369 153
Fragments arranged by size:
3875 1369 333 153
BstYI r'GATC_y
Cuts at: 0 193 541 777 1428 1509 1821 3133 5133
Size: 193 348 236 651 81 312 1312 2000
Cuts at: 5133 5392 5730
Size: 259 338
Fragments arranged by size:
2000 1312 651 348 338 312 259 236
193 81
Bsu36I CC'TnA_GG
Cuts at: 0 292 860 1519 1715 5730
Size: 292 568 659 196 4015
Fragments arranged by size:
4015 659 568 292 196
BtgI C'CryG_G
Cuts at: 0 157 4684 5730
Size: 157 4527 1046
Fragments arranged by size:
4527 1046 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4023 5730
Size: 4023 1707
BtsI GCAGTG_nn'
Cuts at: 0 2771 4754 4976 5493 5730
Size: 2771 1983 222 517 237
Fragments arranged by size:
2771 1983 517 237 222
ClaI AT'CG_AT
Cuts at: 0 1458 4563 5730
Size: 1458 3105 1167
Fragments arranged by size:
3105 1458 1167
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2747 2782 5730
Size: 2747 35 2948
Fragments arranged by size:
2948 2747 35
DraI TTT'AAA
Cuts at: 0 689 2540 3317 4160 5103 5730
Size: 689 1851 777 843 943 627
Fragments arranged by size:
1851 943 843 777 689 627
EaeI y'GGCC_r
Cuts at: 0 388 1256 2768 5714 5730
Size: 388 868 1512 2946 16
Fragments arranged by size:
2946 1512 868 388 16
EagI C'GGCC_G
Cuts at: 0 388 5730
Size: 388 5342
EarI CTCTTCn'nnn_
Cuts at: 0 219 237 586 755 1091 1139 1352 1382
Size: 219 18 349 169 336 48 213 30
Cuts at: 1382 1856 2342 2738 2999 3452 5273 5730
Size: 474 486 396 261 453 1821 457
Fragments arranged by size:
1821 486 474 457 453 396 349 336
261 219 213 169 48 30 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 215 329 5730
Size: 215 114 5401
Fragments arranged by size:
5401 215 114
EcoICRI GAG'CTC
Cuts at: 0 365 596 3512 5730
Size: 365 231 2916 2218
Fragments arranged by size:
2916 2218 365 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 489 1120 1400 1490 1607 1667 1838
Size: 15 474 631 280 90 117 60 171
Cuts at: 1838 1952 2333 2416 2612 2659 2831 2978 3098
Size: 114 381 83 196 47 172 147 120
Cuts at: 3098 3701 3888 3983 4277 4864 5730
Size: 603 187 95 294 587 866
Fragments arranged by size:
866 631 603 587 474 381 294 280
196 187 172 171 147 120 117 114
95 90 83 60 47 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 527 5730
Size: 527 5203
EcoO109I rG'GnC_Cy
Cuts at: 0 298 2071 5730
Size: 298 1773 3659
Fragments arranged by size:
3659 1773 298
EcoRI G'AATT_C
Cuts at: 0 4600 5413 5730
Size: 4600 813 317
Fragments arranged by size:
4600 813 317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 79 700 1520 1912 3152 5730
Size: 79 621 820 392 1240 2578
Fragments arranged by size:
2578 1240 820 621 392 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 1054 1086 1090 1122 1924 1956
Size: 96 32 926 32 4 32 802 32
Cuts at: 1956 2037 2069 2137 2169 2473 2505 3640 3672
Size: 81 32 68 32 304 32 1135 32
Cuts at: 3672 3751 3783 3907 3939 5164 5196 5346 5378
Size: 79 32 124 32 1225 32 150 32
Cuts at: 5378 5485 5517 5730
Size: 107 32 213
Fragments arranged by size:
1225 1135 926 802 304 213 150 124
107 96 81 79 68 32 32 32
32 32 32 32 32 32 32 32
32 32 4
HincII GTy'rAC
Cuts at: 0 1202 2177 2652 4662 5730
Size: 1202 975 475 2010 1068
Fragments arranged by size:
2010 1202 1068 975 475
HindIII A'AGCT_T
Cuts at: 0 2240 2671 2720 4096 4305 4754 5730
Size: 2240 431 49 1376 209 449 976
Fragments arranged by size:
2240 1376 976 449 431 209 49
HpaI GTT'AAC
Cuts at: 0 2652 4662 5730
Size: 2652 2010 1068
Fragments arranged by size:
2652 2010 1068
MfeI C'AATT_G
Cuts at: 0 5168 5730
Size: 5168 562
MluI A'CGCG_T
Cuts at: 0 272 5730
Size: 272 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 689 1016 4352 5528 5730
Size: 94 595 327 3336 1176 202
Fragments arranged by size:
3336 1176 595 327 202 94
MscI TGG'CCA
Cuts at: 0 2770 5716 5730
Size: 2770 2946 14
Fragments arranged by size:
2946 2770 14
MslI CAynn'nnrTG
Cuts at: 0 4819 5730
Size: 4819 911
MspA1I CmG'CkG
Cuts at: 0 134 159 246 1368 2394 3309 4279 4458
Size: 134 25 87 1122 1026 915 970 179
Cuts at: 4458 5730
Size: 1272
Fragments arranged by size:
1272 1122 1026 970 915 179 134 87
25
NcoI C'CATG_G
Cuts at: 0 4684 5730
Size: 4684 1046
NdeI CA'TA_TG
Cuts at: 0 5024 5301 5730
Size: 5024 277 429
Fragments arranged by size:
5024 429 277
NsiI A_TGCA'T
Cuts at: 0 724 3205 4512 5730
Size: 724 2481 1307 1218
Fragments arranged by size:
2481 1307 1218 724
NspI r_CATG'y
Cuts at: 0 3581 4725 5711 5730
Size: 3581 1144 986 19
Fragments arranged by size:
3581 1144 986 19
PciI A'CATG_T
Cuts at: 0 3577 4721 5730
Size: 3577 1144 1009
Fragments arranged by size:
3577 1144 1009
PflMI CCAn_nnn'nTGG
Cuts at: 0 4842 4914 5730
Size: 4842 72 816
Fragments arranged by size:
4842 816 72
PfoI T'CCnGG_A
Cuts at: 0 3644 4841 5160 5730
Size: 3644 1197 319 570
Fragments arranged by size:
3644 1197 570 319
PpuMI rG'GwC_Cy
Cuts at: 0 298 2071 5730
Size: 298 1773 3659
Fragments arranged by size:
3659 1773 298
PvuII CAG'CTG
Cuts at: 0 246 2394 3309 4279 4458 5730
Size: 246 2148 915 970 179 1272
Fragments arranged by size:
2148 1272 970 915 246 179
SacI G_AGCT'C
Cuts at: 0 367 598 3514 5730
Size: 367 231 2916 2216
Fragments arranged by size:
2916 2216 367 231
SacII CC_GC'GG
Cuts at: 0 160 5730
Size: 160 5570
SanDI GG'GwC_CC
Cuts at: 0 298 5730
Size: 298 5432
SapI GCTCTTCn'nnn_
Cuts at: 0 219 586 1139 2738 5730
Size: 219 367 553 1599 2992
Fragments arranged by size:
2992 1599 553 367 219
ScaI AGT'ACT
Cuts at: 0 3696 4111 5730
Size: 3696 415 1619
Fragments arranged by size:
3696 1619 415
SfcI C'TryA_G
Cuts at: 0 1274 3227 3368 3465 3926 5730
Size: 1274 1953 141 97 461 1804
Fragments arranged by size:
1953 1804 1274 461 141 97
SmaI CCC'GGG
Cuts at: 0 184 5730
Size: 184 5546
SmlI C'TyrA_G
Cuts at: 0 3172 4093 5730
Size: 3172 921 1637
Fragments arranged by size:
3172 1637 921
SnaBI TAC'GTA
Cuts at: 0 4356 5730
Size: 4356 1374
SspI AAT'ATT
Cuts at: 0 4413 5157 5730
Size: 4413 744 573
Fragments arranged by size:
4413 744 573
StuI AGG'CCT
Cuts at: 0 168 447 4798 5730
Size: 168 279 4351 932
Fragments arranged by size:
4351 932 279 168
StyI C'CwwG_G
Cuts at: 0 419 2041 3491 4145 4247 4684 5576 5730
Size: 419 1622 1450 654 102 437 892 154
Fragments arranged by size:
1622 1450 892 654 437 419 154 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 478 5730
Size: 478 5252
TatI w'GTAC_w
Cuts at: 0 1218 1240 1451 3694 4080 4109 5340 5730
Size: 1218 22 211 2243 386 29 1231 390
Fragments arranged by size:
2243 1231 1218 390 386 211 29 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1747 1779 5730
Size: 1747 32 3951
Fragments arranged by size:
3951 1747 32
Tth111I GACn'n_nGTC
Cuts at: 0 2075 5730
Size: 2075 3655
XmaI C'CCGG_G
Cuts at: 0 182 5730
Size: 182 5548
XmnI GAAnn'nnTTC
Cuts at: 0 2621 2639 2684 2868 3245 3500 5730
Size: 2621 18 45 184 377 255 2230
Fragments arranged by size:
2621 2230 377 255 184 45 18
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AlwNI ApaLI
ApoI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BciVI BfrBI BglII BlpI Bme1580I BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NsiI NspI PciI PflMI PfoI PpuMI PvuII SacI
SacII SanDI SapI ScaI SfcI SmaI SmlI SnaBI
SspI StuI StyI TaqII TatI TstI Tth111I XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AleI AlfI AloI
ApaI AscI AseI AsiSI BbeI BcgI BclI BglI
BmgBI BmrI BmtI BplI BsaBI BsaHI BsiWI BsmBI
BspEI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
DraIII DrdI EcoRV FseI FspI FspAI HaeII KasI
KpnI NaeI NarI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SalI SbfI SexAI SfiI
SfoI SgfI SgrAI SpeI SphI SrfI SwaI XbaI
XcmI XhoI ZraI