Restriction map for
pF1KSDA0550
(Linear) (Six-base) MAPSORT of: KIAA0550.seq Check: 5496 from: 1 to: 4566
KIAA0550.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 598 924 1762 2016 4566
Size: 598 326 838 254 2550
Fragments arranged by size:
2550 838 598 326 254
AccI GT'mk_AC
Cuts at: 0 577 3575 4566
Size: 577 2998 991
Fragments arranged by size:
2998 991 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2615 3716 4566
Size: 2615 1101 850
Fragments arranged by size:
2615 1101 850
AflIII A'CryG_T
Cuts at: 0 904 1078 4566
Size: 904 174 3488
Fragments arranged by size:
3488 904 174
AhdI GACnn_n'nnGTC
Cuts at: 0 627 4566
Size: 627 3939
AleI CACnn'nnGTG
Cuts at: 0 2242 4147 4566
Size: 2242 1905 419
Fragments arranged by size:
2242 1905 419
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1298 3492 4566
Size: 1298 2194 1074
Fragments arranged by size:
2194 1298 1074
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 891 923 1053 1085 1383 1415 4566
Size: 891 32 130 32 298 32 3151
Fragments arranged by size:
3151 891 298 130 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 633 699 1643 2137 3091 4566
Size: 633 66 944 494 954 1475
Fragments arranged by size:
1475 954 944 633 494 66
ApaLI G'TGCA_C
Cuts at: 0 1278 4566
Size: 1278 3288
ApoI r'AATT_y
Cuts at: 0 214 375 757 850 1827 2079 4042 4122
Size: 214 161 382 93 977 252 1963 80
Cuts at: 4122 4155 4330 4521 4566
Size: 33 175 191 45
Fragments arranged by size:
1963 977 382 252 214 191 175 161
93 80 45 33
AseI AT'TA_AT
Cuts at: 0 2348 4566
Size: 2348 2218
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 938 971 4566
Size: 938 33 3595
Fragments arranged by size:
3595 938 33
BamHI G'GATC_C
Cuts at: 0 3498 4566
Size: 3498 1068
BanI G'GyrC_C
Cuts at: 0 1627 4566
Size: 1627 2939
BanII G_rGCy'C
Cuts at: 0 3304 4566
Size: 3304 1262
BbsI GAAGACnn'nnnn_
Cuts at: 0 1648 4566
Size: 1648 2918
BbvCI CC'TCA_GC
Cuts at: 0 596 4566
Size: 596 3970
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3500 3534 4566
Size: 3500 34 1032
Fragments arranged by size:
3500 1032 34
BclI T'GATC_A
Cuts at: 0 270 3027 4566
Size: 270 2757 1539
Fragments arranged by size:
2757 1539 270
BfrBI ATG'CAT
Cuts at: 0 1700 2536 3469 4284 4566
Size: 1700 836 933 815 282
Fragments arranged by size:
1700 933 836 815 282
BglI GCCn_nnn'nGGC
Cuts at: 0 2584 4566
Size: 2584 1982
BglII A'GATC_T
Cuts at: 0 2310 4566
Size: 2310 2256
BlpI GC'TnA_GC
Cuts at: 0 2588 3703 3793 4566
Size: 2588 1115 90 773
Fragments arranged by size:
2588 1115 773 90
Bme1580I G_kGCm'C
Cuts at: 0 1282 4566
Size: 1282 3284
BmgBI CAC'GTC
Cuts at: 0 3335 4566
Size: 3335 1231
BmrI ACTGGGnnnn_n'
Cuts at: 0 888 1186 2199 4421 4566
Size: 888 298 1013 2222 145
Fragments arranged by size:
2222 1013 888 298 145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3079 3218 4566
Size: 3079 139 1348
Fragments arranged by size:
3079 1348 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1565 2428 4566
Size: 1565 863 2138
Fragments arranged by size:
2138 1565 863
Bpu10I CC'TnA_GC
Cuts at: 0 232 596 1751 4566
Size: 232 364 1155 2815
Fragments arranged by size:
2815 1155 364 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3881 4566
Size: 3881 685
BsaBI GATnn'nnATC
Cuts at: 0 1701 4566
Size: 1701 2865
BsaHI Gr'CG_yC
Cuts at: 0 3289 4566
Size: 3289 1277
BsaWI w'CCGG_w
Cuts at: 0 2241 4566
Size: 2241 2325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 891 921 1036 1053 1066 1083 1383 1413
Size: 891 30 115 17 13 17 300 30
Cuts at: 1413 3291 3321 4566
Size: 1878 30 1245
Fragments arranged by size:
1878 1245 891 300 115 30 30 30
17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1112 4566
Size: 1112 3454
BseYI C'CCAG_C
Cuts at: 0 1421 4566
Size: 1421 3145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1472 1508 2805 4566
Size: 147 1325 36 1297 1761
Fragments arranged by size:
1761 1325 1297 147 36
BsiHKAI G_wGCw'C
Cuts at: 0 904 1237 1249 1282 1745 3304 4566
Size: 904 333 12 33 463 1559 1262
Fragments arranged by size:
1559 1262 904 463 333 33 12
BsmI GAATG_Cn'
Cuts at: 0 351 1312 1597 1623 1728 1769 3443 4566
Size: 351 961 285 26 105 41 1674 1123
Fragments arranged by size:
1674 1123 961 351 285 105 41 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2569 3296 4566
Size: 2569 727 1270
Fragments arranged by size:
2569 1270 727
Bsp1286I G_dGCh'C
Cuts at: 0 904 1237 1249 1282 1745 3304 4566
Size: 904 333 12 33 463 1559 1262
Fragments arranged by size:
1559 1262 904 463 333 33 12
BspHI T'CATG_A
Cuts at: 0 810 3551 4566
Size: 810 2741 1015
Fragments arranged by size:
2741 1015 810
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 598 924 1762 2016 3090 4566
Size: 26 572 326 838 254 1074 1476
Fragments arranged by size:
1476 1074 838 572 326 254 26
BsrDI GCAATG_nn'
Cuts at: 0 1477 3409 3578 3715 4566
Size: 1477 1932 169 137 851
Fragments arranged by size:
1932 1477 851 169 137
BsrFI r'CCGG_y
Cuts at: 0 1271 3184 4566
Size: 1271 1913 1382
Fragments arranged by size:
1913 1382 1271
BsrGI T'GTAC_A
Cuts at: 0 1354 4566
Size: 1354 3212
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1337 1643 1764 4125 4480 4566
Size: 1337 306 121 2361 355 86
Fragments arranged by size:
2361 1337 355 306 121 86
BstEII G'GTnAC_C
Cuts at: 0 1052 1791 4566
Size: 1052 739 2775
Fragments arranged by size:
2775 1052 739
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4505 4566
Size: 4505 61
BstYI r'GATC_y
Cuts at: 0 186 297 1862 2310 2728 3498 4566
Size: 186 111 1565 448 418 770 1068
Fragments arranged by size:
1565 1068 770 448 418 186 111
BstZ17I GTA'TAC
Cuts at: 0 578 4566
Size: 578 3988
Bsu36I CC'TnA_GG
Cuts at: 0 827 1544 4566
Size: 827 717 3022
Fragments arranged by size:
3022 827 717
BtgI C'CryG_G
Cuts at: 0 1057 1293 1318 3284 4405 4566
Size: 1057 236 25 1966 1121 161
Fragments arranged by size:
1966 1121 1057 236 161 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1530 4566
Size: 1530 3036
BtsI GCAGTG_nn'
Cuts at: 0 972 1013 1280 1282 1460 1512 2658 2831
Size: 972 41 267 2 178 52 1146 173
Cuts at: 2831 3438 4566
Size: 607 1128
Fragments arranged by size:
1146 1128 972 607 267 178 173 52
41 2
DraI TTT'AAA
Cuts at: 0 1890 4566
Size: 1890 2676
DrdI GACnn_nn'nnGTC
Cuts at: 0 3339 4566
Size: 3339 1227
EaeI y'GGCC_r
Cuts at: 0 2984 3353 4566
Size: 2984 369 1213
Fragments arranged by size:
2984 1213 369
EarI CTCTTCn'nnn_
Cuts at: 0 878 4288 4566
Size: 878 3410 278
Fragments arranged by size:
3410 878 278
EcoICRI GAG'CTC
Cuts at: 0 3302 4566
Size: 3302 1264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1565 2428 2615 3716 4566
Size: 1565 863 187 1101 850
Fragments arranged by size:
1565 1101 863 850 187
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 1750 4566
Size: 691 1059 2816
Fragments arranged by size:
2816 1059 691
EcoO109I rG'GnC_Cy
Cuts at: 0 228 822 1150 1439 3079 4566
Size: 228 594 328 289 1640 1487
Fragments arranged by size:
1640 1487 594 328 289 228
EcoRI G'AATT_C
Cuts at: 0 2079 4042 4566
Size: 2079 1963 524
Fragments arranged by size:
2079 1963 524
EcoRV GAT'ATC
Cuts at: 0 4220 4566
Size: 4220 346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1762 4566
Size: 1762 2804
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 613 645 759 791 1036 1068
Size: 433 32 148 32 114 32 245 32
Cuts at: 1068 1768 1800 2254 2286 2551 2583 2591 2623
Size: 700 32 454 32 265 32 8 32
Cuts at: 2623 3140 3172 3355 3387 3477 3509 4566
Size: 517 32 183 32 90 32 1057
Fragments arranged by size:
1057 700 517 454 433 265 245 183
148 114 90 32 32 32 32 32
32 32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 0 2187 3105 4566
Size: 2187 918 1461
Fragments arranged by size:
2187 1461 918
HindIII A'AGCT_T
Cuts at: 0 2122 4566
Size: 2122 2444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 427 503 1787 2034 2769 4154 4566
Size: 214 213 76 1284 247 735 1385 412
Fragments arranged by size:
1385 1284 735 412 247 214 213 76
MscI TGG'CCA
Cuts at: 0 2986 3355 4566
Size: 2986 369 1211
Fragments arranged by size:
2986 1211 369
MslI CAynn'nnrTG
Cuts at: 0 1077 1391 2242 3474 3775 3781 4147 4566
Size: 1077 314 851 1232 301 6 366 419
Fragments arranged by size:
1232 1077 851 419 366 314 301 6
MspA1I CmG'CkG
Cuts at: 0 980 1288 3091 4147 4566
Size: 980 308 1803 1056 419
Fragments arranged by size:
1803 1056 980 419 308
NaeI GCC'GGC
Cuts at: 0 1273 4566
Size: 1273 3293
NcoI C'CATG_G
Cuts at: 0 1057 1293 1318 3284 4405 4566
Size: 1057 236 25 1966 1121 161
Fragments arranged by size:
1966 1121 1057 236 161 25
NdeI CA'TA_TG
Cuts at: 0 4115 4301 4566
Size: 4115 186 265
Fragments arranged by size:
4115 265 186
NgoMIV G'CCGG_C
Cuts at: 0 1271 4566
Size: 1271 3295
NsiI A_TGCA'T
Cuts at: 0 1702 2538 3471 4286 4566
Size: 1702 836 933 815 280
Fragments arranged by size:
1702 933 836 815 280
NspI r_CATG'y
Cuts at: 0 908 1082 3544 3774 4566
Size: 908 174 2462 230 792
Fragments arranged by size:
2462 908 792 230 174
PasI CC'CwG_GG
Cuts at: 0 2508 4566
Size: 2508 2058
PciI A'CATG_T
Cuts at: 0 904 1078 4566
Size: 904 174 3488
Fragments arranged by size:
3488 904 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 1198 4566
Size: 1198 3368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 891 923 1053 1085 1383 1415 4566
Size: 891 32 130 32 298 32 3151
Fragments arranged by size:
3151 891 298 130 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 228 822 1150 1439 3079 4566
Size: 228 594 328 289 1640 1487
Fragments arranged by size:
1640 1487 594 328 289 228
PsiI TTA'TAA
Cuts at: 0 2935 4566
Size: 2935 1631
PstI C_TGCA'G
Cuts at: 0 979 1287 2133 3087 4566
Size: 979 308 846 954 1479
Fragments arranged by size:
1479 979 954 846 308
PvuII CAG'CTG
Cuts at: 0 1288 4147 4566
Size: 1288 2859 419
Fragments arranged by size:
2859 1288 419
SacI G_AGCT'C
Cuts at: 0 3304 4566
Size: 3304 1262
SanDI GG'GwC_CC
Cuts at: 0 822 4566
Size: 822 3744
SfcI C'TryA_G
Cuts at: 0 361 975 1283 2129 3083 4566
Size: 361 614 308 846 954 1483
Fragments arranged by size:
1483 954 846 614 361 308
SmlI C'TyrA_G
Cuts at: 0 3896 4566
Size: 3896 670
SphI G_CATG'C
Cuts at: 0 3544 4566
Size: 3544 1022
SspI AAT'ATT
Cuts at: 0 1179 3987 4566
Size: 1179 2808 579
Fragments arranged by size:
2808 1179 579
StuI AGG'CCT
Cuts at: 0 2379 4566
Size: 2379 2187
StyI C'CwwG_G
Cuts at: 0 1057 1293 1318 2955 3284 4405 4566
Size: 1057 236 25 1637 329 1121 161
Fragments arranged by size:
1637 1121 1057 329 236 161 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 724 762 2521 4566
Size: 724 38 1759 2045
Fragments arranged by size:
2045 1759 724 38
TatI w'GTAC_w
Cuts at: 0 514 1029 1354 1712 3842 4566
Size: 514 515 325 358 2130 724
Fragments arranged by size:
2130 724 515 514 358 325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2090 2122 3291 3323 4566
Size: 2090 32 1169 32 1243
Fragments arranged by size:
2090 1243 1169 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3886 4566
Size: 3886 680
XbaI T'CTAG_A
Cuts at: 0 1956 4566
Size: 1956 2610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 1803 2361 3282 4566
Size: 45 1758 558 921 1284
Fragments arranged by size:
1758 1284 921 558 45
XmnI GAAnn'nnTTC
Cuts at: 0 140 349 3645 4156 4310 4566
Size: 140 209 3296 511 154 256
Fragments arranged by size:
3296 511 256 209 154 140
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AseI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PpiI PpuMI PsiI PstI PvuII
SacI SanDI SfcI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI ApaI AscI
AsiSI AvaI AvrII BbeI BciVI BmtI BsaI BsaAI
BsiEI BsiWI BspEI BsrBI BssHII BssSI BstBI ClaI
CspCI DraIII EagI EciI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NarI NheI NotI
NruI PacI PfoI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI XhoI XmaI ZraI