Restriction map for
pF1KA0551
(Linear) (Six-base) MAPSORT of: KIAA0551.seq Check: 7734 from: 1 to: 4080
KIAA0551.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 437 4080
Size: 437 3643
AatII G_ACGT'C
Cuts at: 0 2725 2953 4080
Size: 2725 228 1127
Fragments arranged by size:
2725 1127 228
AccI GT'mk_AC
Cuts at: 0 1042 2373 2942 3432 4080
Size: 1042 1331 569 490 648
Fragments arranged by size:
1331 1042 648 569 490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1492 1695 4080
Size: 778 714 203 2385
Fragments arranged by size:
2385 778 714 203
AflIII A'CryG_T
Cuts at: 0 212 368 4080
Size: 212 156 3712
Fragments arranged by size:
3712 212 156
AhdI GACnn_n'nnGTC
Cuts at: 0 648 3292 4080
Size: 648 2644 788
Fragments arranged by size:
2644 788 648
AleI CACnn'nnGTG
Cuts at: 0 3853 4080
Size: 3853 227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 414 4080
Size: 414 3666
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1870 1902 4080
Size: 1870 32 2178
Fragments arranged by size:
2178 1870 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1116 1161 1395 2566 4080
Size: 1116 45 234 1171 1514
Fragments arranged by size:
1514 1171 1116 234 45
ApaLI G'TGCA_C
Cuts at: 0 1831 4080
Size: 1831 2249
ApoI r'AATT_y
Cuts at: 0 781 2058 3466 3532 4062 4080
Size: 781 1277 1408 66 530 18
Fragments arranged by size:
1408 1277 781 530 66 18
BamHI G'GATC_C
Cuts at: 0 2257 4080
Size: 2257 1823
BanI G'GyrC_C
Cuts at: 0 585 2013 4080
Size: 585 1428 2067
Fragments arranged by size:
2067 1428 585
BanII G_rGCy'C
Cuts at: 0 723 1013 2147 2161 2604 3516 3963 4080
Size: 723 290 1134 14 443 912 447 117
Fragments arranged by size:
1134 912 723 447 443 290 117 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 2003 4080
Size: 2003 2077
BclI T'GATC_A
Cuts at: 0 347 3245 4080
Size: 347 2898 835
Fragments arranged by size:
2898 835 347
BfrBI ATG'CAT
Cuts at: 0 3951 4080
Size: 3951 129
BglII A'GATC_T
Cuts at: 0 39 1376 3558 4080
Size: 39 1337 2182 522
Fragments arranged by size:
2182 1337 522 39
BlpI GC'TnA_GC
Cuts at: 0 527 4080
Size: 527 3553
Bme1580I G_kGCm'C
Cuts at: 0 1835 4080
Size: 1835 2245
BmgBI CAC'GTC
Cuts at: 0 3851 4080
Size: 3851 229
BmrI ACTGGGnnnn_n'
Cuts at: 0 1818 2363 2848 4080
Size: 1818 545 485 1232
Fragments arranged by size:
1818 1232 545 485
BmtI G_CTAG'C
Cuts at: 0 2384 4080
Size: 2384 1696
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1017 1032 2828 2889 4080
Size: 1017 15 1796 61 1191
Fragments arranged by size:
1796 1191 1017 61 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 1234 2175 2181 2478 2588 2674 2747
Size: 1138 96 941 6 297 110 86 73
Cuts at: 2747 2798 3547 4043 4080
Size: 51 749 496 37
Fragments arranged by size:
1138 941 749 496 297 110 96 86
73 51 37 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 4080
Size: 889 3191
BsaI GGTCTCn'nnnn_
Cuts at: 0 863 2539 2650 4080
Size: 863 1676 111 1430
Fragments arranged by size:
1676 1430 863 111
BsaBI GATnn'nnATC
Cuts at: 0 2405 4080
Size: 2405 1675
BsaHI Gr'CG_yC
Cuts at: 0 2722 2950 4080
Size: 2722 228 1130
Fragments arranged by size:
2722 1130 228
BsaWI w'CCGG_w
Cuts at: 0 236 2132 2441 3251 3911 4080
Size: 236 1896 309 810 660 169
Fragments arranged by size:
1896 810 660 309 236 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1870 1900 2476 2506 2586 2616 4080
Size: 1870 30 576 30 80 30 1464
Fragments arranged by size:
1870 1464 576 80 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 953 1003 1006 1156 1201 1231 1297
Size: 394 559 50 3 150 45 30 66
Cuts at: 1297 1315 1318 1323 1369 1372 1931 2478 2481
Size: 18 3 5 46 3 559 547 3
Cuts at: 2481 2515 2539 3707 4080
Size: 34 24 1168 373
Fragments arranged by size:
1168 559 559 547 394 373 150 66
50 46 45 34 30 24 18 5
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1012 2040 2213 2218 2237 3887 4080
Size: 1012 1028 173 5 19 1650 193
Fragments arranged by size:
1650 1028 1012 193 173 19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 2818 3176 4080
Size: 415 2403 358 904
Fragments arranged by size:
2403 904 415 358
BsiHKAI G_wGCw'C
Cuts at: 0 528 690 723 1148 1835 2082 2604 3830
Size: 528 162 33 425 687 247 522 1226
Cuts at: 3830 3963 4080
Size: 133 117
Fragments arranged by size:
1226 687 528 522 425 247 162 133
117 33
BsmI GAATG_Cn'
Cuts at: 0 896 3495 4080
Size: 896 2599 585
Fragments arranged by size:
2599 896 585
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2713 2853 2957 4080
Size: 2713 140 104 1123
Fragments arranged by size:
2713 1123 140 104
Bsp1286I G_dGCh'C
Cuts at: 0 528 690 723 1013 1148 1835 2082 2147
Size: 528 162 33 290 135 687 247 65
Cuts at: 2147 2161 2604 3516 3830 3963 4080
Size: 14 443 912 314 133 117
Fragments arranged by size:
912 687 528 443 314 290 247 162
135 133 117 65 33 14
BspEI T'CCGG_A
Cuts at: 0 2132 2441 4080
Size: 2132 309 1639
Fragments arranged by size:
2132 1639 309
BspHI T'CATG_A
Cuts at: 0 4046 4080
Size: 4046 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 1697 1815 4080
Size: 437 1260 118 2265
Fragments arranged by size:
2265 1260 437 118
BsrDI GCAATG_nn'
Cuts at: 0 2766 4080
Size: 2766 1314
BsrFI r'CCGG_y
Cuts at: 0 1271 1631 4080
Size: 1271 360 2449
Fragments arranged by size:
2449 1271 360
BssSI C'ACGA_G
Cuts at: 0 1834 4080
Size: 1834 2246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 685 1395 4080
Size: 685 710 2685
Fragments arranged by size:
2685 710 685
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 3855 4080
Size: 675 3180 225
Fragments arranged by size:
3180 675 225
BstYI r'GATC_y
Cuts at: 0 39 68 1376 1772 1920 2098 2257 2406
Size: 39 29 1308 396 148 178 159 149
Cuts at: 2406 3558 3908 4080
Size: 1152 350 172
Fragments arranged by size:
1308 1152 396 350 178 172 159 149
148 39 29
BstZ17I GTA'TAC
Cuts at: 0 2943 3433 4080
Size: 2943 490 647
Fragments arranged by size:
2943 647 490
BtsI GCAGTG_nn'
Cuts at: 0 2184 4080
Size: 2184 1896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1938 1973 4080
Size: 1938 35 2107
Fragments arranged by size:
2107 1938 35
DraIII CAC_nnn'GTG
Cuts at: 0 1896 4080
Size: 1896 2184
DrdI GACnn_nn'nnGTC
Cuts at: 0 517 4080
Size: 517 3563
EaeI y'GGCC_r
Cuts at: 0 1076 1163 2745 2772 3804 4080
Size: 1076 87 1582 27 1032 276
Fragments arranged by size:
1582 1076 1032 276 87 27
EarI CTCTTCn'nnn_
Cuts at: 0 179 730 980 2516 2966 4080
Size: 179 551 250 1536 450 1114
Fragments arranged by size:
1536 1114 551 450 250 179
EcoICRI GAG'CTC
Cuts at: 0 721 2602 3961 4080
Size: 721 1881 1359 119
Fragments arranged by size:
1881 1359 721 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1138 1234 1492 1695 2175 2181 2478
Size: 778 360 96 258 203 480 6 297
Cuts at: 2478 2588 2674 2747 2798 3547 4043 4080
Size: 110 86 73 51 749 496 37
Fragments arranged by size:
778 749 496 480 360 297 258 203
110 96 86 73 51 37 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2166 4080
Size: 2166 1914
EcoO109I rG'GnC_Cy
Cuts at: 0 57 1098 1804 1851 2086 4080
Size: 57 1041 706 47 235 1994
Fragments arranged by size:
1994 1041 706 235 57 47
EcoRI G'AATT_C
Cuts at: 0 2058 4080
Size: 2058 2022
EcoRV GAT'ATC
Cuts at: 0 1676 3681 4080
Size: 1676 2005 399
Fragments arranged by size:
2005 1676 399
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 660 1429 2045 4080
Size: 660 769 616 2035
Fragments arranged by size:
2035 769 660 616
HaeII r_GCGC'y
Cuts at: 0 751 1272 4080
Size: 751 521 2808
Fragments arranged by size:
2808 751 521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 634 666 840 872 1764 1796 1912 1944
Size: 634 32 174 32 892 32 116 32
Cuts at: 1944 1960 1992 2473 2505 2968 2971 3000 3003
Size: 16 32 481 32 463 3 29 3
Cuts at: 3003 3278 3310 3898 3930 4080
Size: 275 32 588 32 150
Fragments arranged by size:
892 634 588 481 463 275 174 150
116 32 32 32 32 32 32 32
32 29 16 3 3
HincII GTy'rAC
Cuts at: 0 1043 2374 4080
Size: 1043 1331 1706
Fragments arranged by size:
1706 1331 1043
MfeI C'AATT_G
Cuts at: 0 844 1435 4080
Size: 844 591 2645
Fragments arranged by size:
2645 844 591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 1682 4080
Size: 72 1610 2398
Fragments arranged by size:
2398 1610 72
MscI TGG'CCA
Cuts at: 0 1078 2747 2774 4080
Size: 1078 1669 27 1306
Fragments arranged by size:
1669 1306 1078 27
MslI CAynn'nnrTG
Cuts at: 0 673 3853 3935 4080
Size: 673 3180 82 145
Fragments arranged by size:
3180 673 145 82
MspA1I CmG'CkG
Cuts at: 0 1074 1116 1131 1158 1518 2681 2818 4080
Size: 1074 42 15 27 360 1163 137 1262
Fragments arranged by size:
1262 1163 1074 360 137 42 27 15
NaeI GCC'GGC
Cuts at: 0 1273 4080
Size: 1273 2807
NdeI CA'TA_TG
Cuts at: 0 627 4080
Size: 627 3453
NgoMIV G'CCGG_C
Cuts at: 0 1271 4080
Size: 1271 2809
NheI G'CTAG_C
Cuts at: 0 2380 4080
Size: 2380 1700
NsiI A_TGCA'T
Cuts at: 0 3953 4080
Size: 3953 127
NspI r_CATG'y
Cuts at: 0 216 708 4080
Size: 216 492 3372
Fragments arranged by size:
3372 492 216
PasI CC'CwG_GG
Cuts at: 0 1800 4080
Size: 1800 2280
PciI A'CATG_T
Cuts at: 0 212 4080
Size: 212 3868
PflMI CCAn_nnn'nTGG
Cuts at: 0 1896 2868 4080
Size: 1896 972 1212
Fragments arranged by size:
1896 1212 972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1870 1902 4080
Size: 1870 32 2178
Fragments arranged by size:
2178 1870 32
PpuMI rG'GwC_Cy
Cuts at: 0 57 1804 2086 4080
Size: 57 1747 282 1994
Fragments arranged by size:
1994 1747 282 57
PsiI TTA'TAA
Cuts at: 0 119 3440 4080
Size: 119 3321 640
Fragments arranged by size:
3321 640 119
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1394 1426 4080
Size: 1394 32 2654
Fragments arranged by size:
2654 1394 32
PstI C_TGCA'G
Cuts at: 0 66 403 1073 1469 4080
Size: 66 337 670 396 2611
Fragments arranged by size:
2611 670 396 337 66
PvuII CAG'CTG
Cuts at: 0 1074 1116 1158 2818 4080
Size: 1074 42 42 1660 1262
Fragments arranged by size:
1660 1262 1074 42 42
SacI G_AGCT'C
Cuts at: 0 723 2604 3963 4080
Size: 723 1881 1359 117
Fragments arranged by size:
1881 1359 723 117
SalI G'TCGA_C
Cuts at: 0 1041 2372 4080
Size: 1041 1331 1708
Fragments arranged by size:
1708 1331 1041
SanDI GG'GwC_CC
Cuts at: 0 57 2086 4080
Size: 57 2029 1994
Fragments arranged by size:
2029 1994 57
SbfI CC_TGCA'GG
Cuts at: 0 66 4080
Size: 66 4014
SexAI A'CCwGG_T
Cuts at: 0 2792 4080
Size: 2792 1288
SfcI C'TryA_G
Cuts at: 0 62 399 1069 1465 2396 4080
Size: 62 337 670 396 931 1684
Fragments arranged by size:
1684 931 670 396 337 62
SmlI C'TyrA_G
Cuts at: 0 904 4080
Size: 904 3176
SpeI A'CTAG_T
Cuts at: 0 504 4080
Size: 504 3576
SspI AAT'ATT
Cuts at: 0 226 243 4080
Size: 226 17 3837
Fragments arranged by size:
3837 226 17
StuI AGG'CCT
Cuts at: 0 1533 4080
Size: 1533 2547
StyI C'CwwG_G
Cuts at: 0 1601 1696 2226 2330 4080
Size: 1601 95 530 104 1750
Fragments arranged by size:
1750 1601 530 104 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1985 3230 4080
Size: 1985 1245 850
Fragments arranged by size:
1985 1245 850
TatI w'GTAC_w
Cuts at: 0 965 2621 4080
Size: 965 1656 1459
Fragments arranged by size:
1656 1459 965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2061 2093 2163 2195 2294 2326 4080
Size: 2061 32 70 32 99 32 1754
Fragments arranged by size:
2061 1754 99 70 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 2815 3879 4080
Size: 162 2653 1064 201
Fragments arranged by size:
2653 1064 201 162
XmnI GAAnn'nnTTC
Cuts at: 0 559 2000 2028 2058 3057 3133 4080
Size: 559 1441 28 30 999 76 947
Fragments arranged by size:
1441 999 947 559 76 30 28
ZraI GAC'GTC
Cuts at: 0 2723 2951 4080
Size: 2723 228 1129
Fragments arranged by size:
2723 1129 228
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI BamHI BanI BanII BbsI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BstZ17I BtsI CspCI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII MfeI MmeI MscI MslI MspA1I NaeI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PpiI
PpuMI PsiI PsrI PstI PvuII SacI SalI SanDI
SbfI SexAI SfcI SmlI SpeI SspI StuI StyI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BglI Bpu10I BsaAI BsiEI BsiWI BsrBI BsrGI BssHII
BstBI BstEII Bsu36I BtgI BtgZI ClaI DraI EagI
EciI FseI FspI FspAI HindIII HpaI KasI KpnI
MluI NarI NcoI NotI NruI PacI PfoI PmeI
PmlI PshAI PspOMI PspXI PvuI RsrII SacII SapI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI