Restriction map for
pF1KA0556
(Linear) (Six-base) MAPSORT of: KIAA0556.seq Check: 7733 from: 1 to: 4854
KIAA0556.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3740 4854
Size: 3740 1114
AatII G_ACGT'C
Cuts at: 0 4364 4854
Size: 4364 490
AccI GT'mk_AC
Cuts at: 0 2947 4854
Size: 2947 1907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 362 3619 4854
Size: 109 253 3257 1235
Fragments arranged by size:
3257 1235 253 109
AfeI AGC'GCT
Cuts at: 0 2926 4854
Size: 2926 1928
AflII C'TTAA_G
Cuts at: 0 351 4854
Size: 351 4503
AflIII A'CryG_T
Cuts at: 0 4418 4627 4854
Size: 4418 209 227
Fragments arranged by size:
4418 227 209
AgeI A'CCGG_T
Cuts at: 0 3012 4854
Size: 3012 1842
AhdI GACnn_n'nnGTC
Cuts at: 0 2073 2631 2943 2968 4854
Size: 2073 558 312 25 1886
Fragments arranged by size:
2073 1886 558 312 25
AleI CACnn'nnGTG
Cuts at: 0 775 4854
Size: 775 4079
AlwNI CAG_nnn'CTG
Cuts at: 0 15 433 543 2884 3720 4113 4854
Size: 15 418 110 2341 836 393 741
Fragments arranged by size:
2341 836 741 418 393 110 15
ApoI r'AATT_y
Cuts at: 0 787 825 2046 3221 3642 4854
Size: 787 38 1221 1175 421 1212
Fragments arranged by size:
1221 1212 1175 787 421 38
AvaI C'yCGr_G
Cuts at: 0 2242 2332 2757 3397 3775 3955 4854
Size: 2242 90 425 640 378 180 899
Fragments arranged by size:
2242 899 640 425 378 180 90
BamHI G'GATC_C
Cuts at: 0 3551 4583 4854
Size: 3551 1032 271
Fragments arranged by size:
3551 1032 271
BanI G'GyrC_C
Cuts at: 0 223 401 1756 1924 1942 3820 4176 4854
Size: 223 178 1355 168 18 1878 356 678
Fragments arranged by size:
1878 1355 678 356 223 178 168 18
BanII G_rGCy'C
Cuts at: 0 2189 2569 2704 2756 2780 3348 3569 3683
Size: 2189 380 135 52 24 568 221 114
Cuts at: 3683 3773 4107 4637 4854
Size: 90 334 530 217
Fragments arranged by size:
2189 568 530 380 334 221 217 135
114 90 52 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 632 2627 2630 4854
Size: 632 1995 3 2224
Fragments arranged by size:
2224 1995 632 3
BbvCI CC'TCA_GC
Cuts at: 0 854 1075 4854
Size: 854 221 3779
Fragments arranged by size:
3779 854 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4832 4854
Size: 4832 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 3239 4854
Size: 3239 1615
BclI T'GATC_A
Cuts at: 0 3215 4496 4854
Size: 3215 1281 358
Fragments arranged by size:
3215 1281 358
BfrBI ATG'CAT
Cuts at: 0 3135 4854
Size: 3135 1719
BglI GCCn_nnn'nGGC
Cuts at: 0 4256 4854
Size: 4256 598
BglII A'GATC_T
Cuts at: 0 3740 4854
Size: 3740 1114
BlpI GC'TnA_GC
Cuts at: 0 4106 4854
Size: 4106 748
Bme1580I G_kGCm'C
Cuts at: 0 2170 4646 4854
Size: 2170 2476 208
Fragments arranged by size:
2476 2170 208
BmgBI CAC'GTC
Cuts at: 0 3990 4854
Size: 3990 864
BmrI ACTGGGnnnn_n'
Cuts at: 0 371 1467 2073 2129 3288 4854
Size: 371 1096 606 56 1159 1566
Fragments arranged by size:
1566 1159 1096 606 371 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 630 2205 4854
Size: 630 1575 2649
Fragments arranged by size:
2649 1575 630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 261 490 743 921 1174 1297 1636
Size: 205 56 229 253 178 253 123 339
Cuts at: 1636 2414 2891 4171 4282 4363 4854
Size: 778 477 1280 111 81 491
Fragments arranged by size:
1280 778 491 477 339 253 253 229
205 178 123 111 81 56
Bpu10I CC'TnA_GC
Cuts at: 0 71 644 854 878 1075 4854
Size: 71 573 210 24 197 3779
Fragments arranged by size:
3779 573 210 197 71 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1195 2553 4058 4854
Size: 1195 1358 1505 796
Fragments arranged by size:
1505 1358 1195 796
BsaI GGTCTCn'nnnn_
Cuts at: 0 954 1162 1486 1972 2392 2466 4854
Size: 954 208 324 486 420 74 2388
Fragments arranged by size:
2388 954 486 420 324 208 74
BsaAI yAC'GTr
Cuts at: 0 1434 4854
Size: 1434 3420
BsaBI GATnn'nnATC
Cuts at: 0 1742 4854
Size: 1742 3112
BsaHI Gr'CG_yC
Cuts at: 0 2644 4361 4854
Size: 2644 1717 493
Fragments arranged by size:
2644 1717 493
BsaWI w'CCGG_w
Cuts at: 0 137 1550 3012 4037 4854
Size: 137 1413 1462 1025 817
Fragments arranged by size:
1462 1413 1025 817 137
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 916 946 1435 1465 4854
Size: 916 30 489 30 3389
Fragments arranged by size:
3389 916 489 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1063 1171 1247 1608 1903 2197 2574 3492
Size: 1063 108 76 361 295 294 377 918
Cuts at: 3492 4295 4854
Size: 803 559
Fragments arranged by size:
1063 918 803 559 377 361 295 294
108 76
BseYI C'CCAG_C
Cuts at: 0 795 1575 1686 1833 2884 3484 4118 4226
Size: 795 780 111 147 1051 600 634 108
Cuts at: 4226 4797 4854
Size: 571 57
Fragments arranged by size:
1051 795 780 634 600 571 147 111
108 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 3037 3718 4854
Size: 436 2601 681 1136
Fragments arranged by size:
2601 1136 681 436
BsiEI CG_ry'CG
Cuts at: 0 1220 2721 3494 4854
Size: 1220 1501 773 1360
Fragments arranged by size:
1501 1360 1220 773
BsiHKAI G_wGCw'C
Cuts at: 0 191 937 1031 2142 2681 2704 2756 2780
Size: 191 746 94 1111 539 23 52 24
Cuts at: 2780 3356 3569 3773 4854
Size: 576 213 204 1081
Fragments arranged by size:
1111 1081 746 576 539 213 204 191
94 52 24 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3996 4017 4828 4833 4854
Size: 3996 21 811 5 21
Fragments arranged by size:
3996 811 21 21 5
Bsp1286I G_dGCh'C
Cuts at: 0 191 937 1031 2142 2170 2189 2569 2681
Size: 191 746 94 1111 28 19 380 112
Cuts at: 2681 2704 2756 2780 3348 3356 3569 3683 3773
Size: 23 52 24 568 8 213 114 90
Cuts at: 3773 4107 4637 4646 4854
Size: 334 530 9 208
Fragments arranged by size:
1111 746 568 530 380 334 213 208
191 114 112 94 90 52 28 24
23 19 9 8
BspEI T'CCGG_A
Cuts at: 0 1550 4037 4854
Size: 1550 2487 817
Fragments arranged by size:
2487 1550 817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1639 3740 4574 4825 4854
Size: 1639 2101 834 251 29
Fragments arranged by size:
2101 1639 834 251 29
BsrBI CCG'CTC
Cuts at: 0 2723 3513 4854
Size: 2723 790 1341
Fragments arranged by size:
2723 1341 790
BsrDI GCAATG_nn'
Cuts at: 0 3479 4854
Size: 3479 1375
BsrFI r'CCGG_y
Cuts at: 0 950 1215 2278 2798 3012 4122 4255 4412
Size: 950 265 1063 520 214 1110 133 157
Cuts at: 4412 4854
Size: 442
Fragments arranged by size:
1110 1063 950 520 442 265 214 157
133
BsrGI T'GTAC_A
Cuts at: 0 4574 4854
Size: 4574 280
BssSI C'ACGA_G
Cuts at: 0 47 775 1467 2689 4854
Size: 47 728 692 1222 2165
Fragments arranged by size:
2165 1222 728 692 47
BstBI TT'CG_AA
Cuts at: 0 1671 4854
Size: 1671 3183
BstEII G'GTnAC_C
Cuts at: 0 3090 4854
Size: 3090 1764
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1044 1152 2121 4134 4854
Size: 1044 108 969 2013 720
Fragments arranged by size:
2013 1044 969 720 108
BstYI r'GATC_y
Cuts at: 0 1445 3166 3551 3740 4583 4854
Size: 1445 1721 385 189 843 271
Fragments arranged by size:
1721 1445 843 385 271 189
BtgI C'CryG_G
Cuts at: 0 250 439 1138 1193 1256 1439 1624 3621
Size: 250 189 699 55 63 183 185 1997
Cuts at: 3621 3833 4592 4854
Size: 212 759 262
Fragments arranged by size:
1997 759 699 262 250 212 189 185
183 63 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 617 3903 4854
Size: 617 3286 951
Fragments arranged by size:
3286 951 617
BtsI GCAGTG_nn'
Cuts at: 0 1105 2673 3647 4854
Size: 1105 1568 974 1207
Fragments arranged by size:
1568 1207 1105 974
DraI TTT'AAA
Cuts at: 0 2898 4854
Size: 2898 1956
DraIII CAC_nnn'GTG
Cuts at: 0 1542 3296 4608 4854
Size: 1542 1754 1312 246
Fragments arranged by size:
1754 1542 1312 246
DrdI GACnn_nn'nnGTC
Cuts at: 0 3136 3886 4854
Size: 3136 750 968
Fragments arranged by size:
3136 968 750
EaeI y'GGCC_r
Cuts at: 0 1217 1703 3491 4124 4410 4589 4854
Size: 1217 486 1788 633 286 179 265
Fragments arranged by size:
1788 1217 633 486 286 265 179
EagI C'GGCC_G
Cuts at: 0 1217 3491 4854
Size: 1217 2274 1363
Fragments arranged by size:
2274 1363 1217
EarI CTCTTCn'nnn_
Cuts at: 0 759 1160 1867 3461 4090 4666 4854
Size: 759 401 707 1594 629 576 188
Fragments arranged by size:
1594 759 707 629 576 401 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3886 4672 4854
Size: 3886 786 182
Fragments arranged by size:
3886 786 182
EcoICRI GAG'CTC
Cuts at: 0 2702 2754 2778 3567 3771 4854
Size: 2702 52 24 789 204 1083
Fragments arranged by size:
2702 1083 789 204 52 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 205 261 362 490 743 921 1174
Size: 109 96 56 101 128 253 178 253
Cuts at: 1174 1297 1636 2414 2891 3619 4171 4282 4363
Size: 123 339 778 477 728 552 111 81
Cuts at: 4363 4854
Size: 491
Fragments arranged by size:
778 728 552 491 477 339 253 253
178 128 123 111 109 101 96 81
56
EcoO109I rG'GnC_Cy
Cuts at: 0 165 654 1830 2389 3119 4854
Size: 165 489 1176 559 730 1735
Fragments arranged by size:
1735 1176 730 559 489 165
EcoRI G'AATT_C
Cuts at: 0 2046 4854
Size: 2046 2808
EcoRV GAT'ATC
Cuts at: 0 1548 3051 4854
Size: 1548 1503 1803
Fragments arranged by size:
1803 1548 1503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1189 4854
Size: 1189 3665
HaeII r_GCGC'y
Cuts at: 0 393 1072 2547 2928 3721 4851 4854
Size: 393 679 1475 381 793 1130 3
Fragments arranged by size:
1475 1130 793 679 393 381 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 619 651 1191 1223 1360 1392
Size: 226 32 361 32 540 32 137 32
Cuts at: 1392 1435 1467 2059 2091 2617 2649 2851 2883
Size: 43 32 592 32 526 32 202 32
Cuts at: 2883 2929 2954 2961 2986 3353 3385 3556 3588
Size: 46 25 7 25 367 32 171 32
Cuts at: 3588 4274 4306 4458 4490 4854
Size: 686 32 152 32 364
Fragments arranged by size:
686 592 540 526 367 364 361 226
202 171 152 137 46 43 32 32
32 32 32 32 32 32 32 32
32 32 25 25 7
HincII GTy'rAC
Cuts at: 0 219 1590 1782 1857 2460 4794 4854
Size: 219 1371 192 75 603 2334 60
Fragments arranged by size:
2334 1371 603 219 192 75 60
HindIII A'AGCT_T
Cuts at: 0 1297 1519 1771 2008 4854
Size: 1297 222 252 237 2846
Fragments arranged by size:
2846 1297 252 237 222
MfeI C'AATT_G
Cuts at: 0 175 4854
Size: 175 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1478 2223 2768 4280 4854
Size: 1478 745 545 1512 574
Fragments arranged by size:
1512 1478 745 574 545
MscI TGG'CCA
Cuts at: 0 1705 4591 4854
Size: 1705 2886 263
Fragments arranged by size:
2886 1705 263
MslI CAynn'nnrTG
Cuts at: 0 311 775 1042 1432 2119 2504 3277 3832
Size: 311 464 267 390 687 385 773 555
Cuts at: 3832 4854
Size: 1022
Fragments arranged by size:
1022 773 687 555 464 390 385 311
267
MspA1I CmG'CkG
Cuts at: 0 252 1656 1686 1735 1837 1949 1987 3639
Size: 252 1404 30 49 102 112 38 1652
Cuts at: 3639 4110 4182 4226 4854
Size: 471 72 44 628
Fragments arranged by size:
1652 1404 628 471 252 112 102 72
49 44 38 30
NaeI GCC'GGC
Cuts at: 0 952 1217 2800 4124 4414 4854
Size: 952 265 1583 1324 290 440
Fragments arranged by size:
1583 1324 952 440 290 265
NcoI C'CATG_G
Cuts at: 0 1138 1256 1439 3833 4592 4854
Size: 1138 118 183 2394 759 262
Fragments arranged by size:
2394 1138 759 262 183 118
NdeI CA'TA_TG
Cuts at: 0 3848 4854
Size: 3848 1006
NgoMIV G'CCGG_C
Cuts at: 0 950 1215 2798 4122 4412 4854
Size: 950 265 1583 1324 290 442
Fragments arranged by size:
1583 1324 950 442 290 265
NsiI A_TGCA'T
Cuts at: 0 3137 4854
Size: 3137 1717
NspI r_CATG'y
Cuts at: 0 2133 3135 3139 4422 4631 4854
Size: 2133 1002 4 1283 209 223
Fragments arranged by size:
2133 1283 1002 223 209 4
PciI A'CATG_T
Cuts at: 0 4418 4627 4854
Size: 4418 209 227
Fragments arranged by size:
4418 227 209
PflMI CCAn_nnn'nTGG
Cuts at: 0 1257 1542 1935 2205 3070 4608 4854
Size: 1257 285 393 270 865 1538 246
Fragments arranged by size:
1538 1257 865 393 285 270 246
PfoI T'CCnGG_A
Cuts at: 0 2391 2638 2768 3554 3793 4854
Size: 2391 247 130 786 239 1061
Fragments arranged by size:
2391 1061 786 247 239 130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1891 1923 4854
Size: 1891 32 2931
Fragments arranged by size:
2931 1891 32
PpuMI rG'GwC_Cy
Cuts at: 0 165 654 2389 3119 4854
Size: 165 489 1735 730 1735
Fragments arranged by size:
1735 1735 730 489 165
PshAI GACnn'nnGTC
Cuts at: 0 966 4854
Size: 966 3888
PstI C_TGCA'G
Cuts at: 0 1064 1112 2786 3461 4854
Size: 1064 48 1674 675 1393
Fragments arranged by size:
1674 1393 1064 675 48
PvuII CAG'CTG
Cuts at: 0 1656 1686 1735 1837 1987 3639 4110 4226
Size: 1656 30 49 102 150 1652 471 116
Cuts at: 4226 4854
Size: 628
Fragments arranged by size:
1656 1652 628 471 150 116 102 49
30
SacI G_AGCT'C
Cuts at: 0 2704 2756 2780 3569 3773 4854
Size: 2704 52 24 789 204 1081
Fragments arranged by size:
2704 1081 789 204 52 24
SacII CC_GC'GG
Cuts at: 0 253 4854
Size: 253 4601
SapI GCTCTTCn'nnn_
Cuts at: 0 759 1867 4854
Size: 759 1108 2987
Fragments arranged by size:
2987 1108 759
SbfI CC_TGCA'GG
Cuts at: 0 3461 4854
Size: 3461 1393
ScaI AGT'ACT
Cuts at: 0 3781 4854
Size: 3781 1073
SexAI A'CCwGG_T
Cuts at: 0 2629 4604 4854
Size: 2629 1975 250
Fragments arranged by size:
2629 1975 250
SfcI C'TryA_G
Cuts at: 0 1060 1108 2782 3457 4854
Size: 1060 48 1674 675 1397
Fragments arranged by size:
1674 1397 1060 675 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4256 4854
Size: 4256 598
SmaI CCC'GGG
Cuts at: 0 2244 2759 4854
Size: 2244 515 2095
Fragments arranged by size:
2244 2095 515
SmlI C'TyrA_G
Cuts at: 0 351 1174 2568 3397 4073 4854
Size: 351 823 1394 829 676 781
Fragments arranged by size:
1394 829 823 781 676 351
SspI AAT'ATT
Cuts at: 0 119 2092 2993 3057 4311 4854
Size: 119 1973 901 64 1254 543
Fragments arranged by size:
1973 1254 901 543 119 64
StuI AGG'CCT
Cuts at: 0 2420 3325 3926 4854
Size: 2420 905 601 928
Fragments arranged by size:
2420 928 905 601
StyI C'CwwG_G
Cuts at: 0 1138 1256 1364 1439 2465 3002 3320 3833
Size: 1138 118 108 75 1026 537 318 513
Cuts at: 3833 4592 4854
Size: 759 262
Fragments arranged by size:
1138 1026 759 537 513 318 262 118
108 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1464 1658 1942 2113 2416 3307 3538 4854
Size: 1464 194 284 171 303 891 231 1316
Fragments arranged by size:
1464 1316 891 303 284 231 194 171
TatI w'GTAC_w
Cuts at: 0 3779 4574 4854
Size: 3779 795 280
Fragments arranged by size:
3779 795 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 916 948 3991 4023 4854
Size: 916 32 3043 32 831
Fragments arranged by size:
3043 916 831 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2374 2646 4854
Size: 2374 272 2208
Fragments arranged by size:
2374 2208 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1136 1936 2318 3831 4854
Size: 1136 800 382 1513 1023
Fragments arranged by size:
1513 1136 1023 800 382
XhoI C'TCGA_G
Cuts at: 0 3397 4854
Size: 3397 1457
XmaI C'CCGG_G
Cuts at: 0 2242 2757 4854
Size: 2242 515 2097
Fragments arranged by size:
2242 2097 515
XmnI GAAnn'nnTTC
Cuts at: 0 2046 4854
Size: 2046 2808
ZraI GAC'GTC
Cuts at: 0 4362 4854
Size: 4362 492
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PpiI PpuMI PshAI PstI PvuII SacI
SacII SapI SbfI ScaI SexAI SfcI SfiI SmaI
SmlI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BmtI BsiWI BsmI BspHI
BssHII BstAPI BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspI FspAI HpaI KasI KpnI MluI NarI NheI
NotI NruI PacI PasI PmeI PmlI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI XbaI