Restriction map for
pF1KSDA0558
(Linear) (Six-base) MAPSORT of: KIAA0558.seq Check: 5543 from: 1 to: 3435
KIAA0558.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1434 3435
Size: 1434 2001
AatII G_ACGT'C
Cuts at: 0 112 1229 1304 3435
Size: 112 1117 75 2131
Fragments arranged by size:
2131 1117 112 75
AccI GT'mk_AC
Cuts at: 0 242 3435
Size: 242 3193
AclI AA'CG_TT
Cuts at: 0 1822 3435
Size: 1822 1613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 940 2039 2706 3435
Size: 421 519 1099 667 729
Fragments arranged by size:
1099 729 667 519 421
AflIII A'CryG_T
Cuts at: 0 1460 1670 2025 2231 3435
Size: 1460 210 355 206 1204
Fragments arranged by size:
1460 1204 355 210 206
AhdI GACnn_n'nnGTC
Cuts at: 0 960 3435
Size: 960 2475
AleI CACnn'nnGTG
Cuts at: 0 735 1879 2458 3435
Size: 735 1144 579 977
Fragments arranged by size:
1144 977 735 579
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 685 717 3435
Size: 685 32 2718
Fragments arranged by size:
2718 685 32
AlwNI CAG_nnn'CTG
Cuts at: 0 399 1971 2196 2606 3117 3435
Size: 399 1572 225 410 511 318
Fragments arranged by size:
1572 511 410 399 318 225
ApaI G_GGCC'C
Cuts at: 0 119 222 3435
Size: 119 103 3213
Fragments arranged by size:
3213 119 103
AvaI C'yCGr_G
Cuts at: 0 1163 2279 2846 2995 3435
Size: 1163 1116 567 149 440
Fragments arranged by size:
1163 1116 567 440 149
AvrII C'CTAG_G
Cuts at: 0 2472 3435
Size: 2472 963
BanI G'GyrC_C
Cuts at: 0 6 25 94 101 172 1099 1881 3435
Size: 6 19 69 7 71 927 782 1554
Fragments arranged by size:
1554 927 782 71 69 19 7 6
BanII G_rGCy'C
Cuts at: 0 119 222 674 801 2111 2330 2468 2995
Size: 119 103 452 127 1310 219 138 527
Cuts at: 2995 3435
Size: 440
Fragments arranged by size:
1310 527 452 440 219 138 127 119
103
BbeI G_GCGC'C
Cuts at: 0 29 105 176 3435
Size: 29 76 71 3259
Fragments arranged by size:
3259 76 71 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 738 937 2277 2370 3435
Size: 476 262 199 1340 93 1065
Fragments arranged by size:
1340 1065 476 262 199 93
BbvCI CC'TCA_GC
Cuts at: 0 2303 2816 3435
Size: 2303 513 619
Fragments arranged by size:
2303 619 513
BclI T'GATC_A
Cuts at: 0 1184 3435
Size: 1184 2251
BglI GCCn_nnn'nGGC
Cuts at: 0 3393 3435
Size: 3393 42
BlpI GC'TnA_GC
Cuts at: 0 503 2467 3435
Size: 503 1964 968
Fragments arranged by size:
1964 968 503
Bme1580I G_kGCm'C
Cuts at: 0 119 222 1102 2814 2864 3435
Size: 119 103 880 1712 50 571
Fragments arranged by size:
1712 880 571 119 103 50
BmgBI CAC'GTC
Cuts at: 0 1584 3435
Size: 1584 1851
BmrI ACTGGGnnnn_n'
Cuts at: 0 225 1486 1492 3027 3435
Size: 225 1261 6 1535 408
Fragments arranged by size:
1535 1261 408 225 6
BmtI G_CTAG'C
Cuts at: 0 2554 3435
Size: 2554 881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 712 2111 2437 2443 3078 3435
Size: 250 462 1399 326 6 635 357
Fragments arranged by size:
1399 635 462 357 326 250 6
Bpu10I CC'TnA_GC
Cuts at: 0 2303 2324 2816 3435
Size: 2303 21 492 619
Fragments arranged by size:
2303 619 492 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1675 3389 3435
Size: 1675 1714 46
Fragments arranged by size:
1714 1675 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 828 3240 3360 3435
Size: 828 2412 120 75
Fragments arranged by size:
2412 828 120 75
BsaAI yAC'GTr
Cuts at: 0 1671 2234 3435
Size: 1671 563 1201
Fragments arranged by size:
1671 1201 563
BsaHI Gr'CG_yC
Cuts at: 0 26 102 109 173 225 269 488 1226
Size: 26 76 7 64 52 44 219 738
Cuts at: 1226 1301 2507 3347 3435
Size: 75 1206 840 88
Fragments arranged by size:
1206 840 738 219 88 76 75 64
52 44 26 7
BsaWI w'CCGG_w
Cuts at: 0 302 1253 3257 3435
Size: 302 951 2004 178
Fragments arranged by size:
2004 951 302 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 664 685 715 2749 2779 3356 3386
Size: 634 30 21 30 2034 30 577 30
Cuts at: 3386 3435
Size: 49
Fragments arranged by size:
2034 634 577 49 30 30 30 30
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3364 3435
Size: 3364 71
BseYI C'CCAG_C
Cuts at: 0 42 194 2002 3210 3342 3435
Size: 42 152 1808 1208 132 93
Fragments arranged by size:
1808 1208 152 132 93 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1057 1099 1240 1468 1663 2143 2825
Size: 412 645 42 141 228 195 480 682
Cuts at: 2825 3183 3262 3435
Size: 358 79 173
Fragments arranged by size:
682 645 480 412 358 228 195 173
141 79 42
BsiEI CG_ry'CG
Cuts at: 0 3397 3435
Size: 3397 38
BsiHKAI G_wGCw'C
Cuts at: 0 674 1897 1922 2111 2468 3435
Size: 674 1223 25 189 357 967
Fragments arranged by size:
1223 967 674 357 189 25
BsmI GAATG_Cn'
Cuts at: 0 2070 3435
Size: 2070 1365
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2992 3193 3435
Size: 2992 201 242
Fragments arranged by size:
2992 242 201
Bsp1286I G_dGCh'C
Cuts at: 0 119 222 674 801 1102 1897 1922 2111
Size: 119 103 452 127 301 795 25 189
Cuts at: 2111 2330 2468 2814 2864 2995 3435
Size: 219 138 346 50 131 440
Fragments arranged by size:
795 452 440 346 301 219 189 138
131 127 119 103 50 25
BspHI T'CATG_A
Cuts at: 0 1187 3020 3435
Size: 1187 1833 415
Fragments arranged by size:
1833 1187 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 732 1434 1965 2705 3435
Size: 28 704 702 531 740 730
Fragments arranged by size:
740 730 704 702 531 28
BsrBI CCG'CTC
Cuts at: 0 908 3435
Size: 908 2527
BsrDI GCAATG_nn'
Cuts at: 0 2033 3435
Size: 2033 1402
BsrFI r'CCGG_y
Cuts at: 0 9 1117 3182 3435
Size: 9 1108 2065 253
Fragments arranged by size:
2065 1108 253 9
BsrGI T'GTAC_A
Cuts at: 0 621 3435
Size: 621 2814
BssHII G'CGCG_C
Cuts at: 0 55 3435
Size: 55 3380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 207 2809 3170 3369 3435
Size: 207 2602 361 199 66
Fragments arranged by size:
2602 361 207 199 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 873 1422 3435
Size: 873 549 2013
Fragments arranged by size:
2013 873 549
BstYI r'GATC_y
Cuts at: 0 626 1352 1772 1967 2223 3435
Size: 626 726 420 195 256 1212
Fragments arranged by size:
1212 726 626 420 256 195
Bsu36I CC'TnA_GG
Cuts at: 0 559 3435
Size: 559 2876
BtgI C'CryG_G
Cuts at: 0 790 1280 1415 2367 2975 3318 3435
Size: 790 490 135 952 608 343 117
Fragments arranged by size:
952 790 608 490 343 135 117
BtsI GCAGTG_nn'
Cuts at: 0 730 906 1321 1456 2146 2504 3204 3235
Size: 730 176 415 135 690 358 700 31
Cuts at: 3235 3435
Size: 200
Fragments arranged by size:
730 700 690 415 358 200 176 135
31
DraIII CAC_nnn'GTG
Cuts at: 0 791 1035 3435
Size: 791 244 2400
Fragments arranged by size:
2400 791 244
EaeI y'GGCC_r
Cuts at: 0 782 1091 1246 2246 3146 3180 3315 3394
Size: 782 309 155 1000 900 34 135 79
Cuts at: 3394 3435
Size: 41
Fragments arranged by size:
1000 900 782 309 155 135 79 41
34
EagI C'GGCC_G
Cuts at: 0 3394 3435
Size: 3394 41
EarI CTCTTCn'nnn_
Cuts at: 0 397 1763 3435
Size: 397 1366 1672
Fragments arranged by size:
1672 1366 397
EcoICRI GAG'CTC
Cuts at: 0 672 2109 2466 3435
Size: 672 1437 357 969
Fragments arranged by size:
1437 969 672 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 421 712 940 2039 2111 2437 2443
Size: 250 171 291 228 1099 72 326 6
Cuts at: 2443 2706 3078 3435
Size: 263 372 357
Fragments arranged by size:
1099 372 357 326 291 263 250 228
171 72 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 1036 1525 3435
Size: 313 723 489 1910
Fragments arranged by size:
1910 723 489 313
EcoO109I rG'GnC_Cy
Cuts at: 0 116 396 579 687 854 1502 2058 3323
Size: 116 280 183 108 167 648 556 1265
Cuts at: 3323 3435
Size: 112
Fragments arranged by size:
1265 648 556 280 183 167 116 112
108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1137 3435
Size: 1137 2298
FspI TGC'GCA
Cuts at: 0 1051 3435
Size: 1051 2384
HaeII r_GCGC'y
Cuts at: 0 29 105 176 758 3435
Size: 29 76 71 582 2677
Fragments arranged by size:
2677 582 76 71 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 946 978 3435
Size: 946 32 2457
Fragments arranged by size:
2457 946 32
HincII GTy'rAC
Cuts at: 0 243 603 2628 3435
Size: 243 360 2025 807
Fragments arranged by size:
2025 807 360 243
HpaI GTT'AAC
Cuts at: 0 2628 3435
Size: 2628 807
KasI G'GCGC_C
Cuts at: 0 25 101 172 3435
Size: 25 76 71 3263
Fragments arranged by size:
3263 76 71 25
MfeI C'AATT_G
Cuts at: 0 1242 3435
Size: 1242 2193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 1759 3435
Size: 1178 581 1676
Fragments arranged by size:
1676 1178 581
MscI TGG'CCA
Cuts at: 0 1093 1248 3435
Size: 1093 155 2187
Fragments arranged by size:
2187 1093 155
MslI CAynn'nnrTG
Cuts at: 0 735 883 1295 1582 1879 2372 2396 2458
Size: 735 148 412 287 297 493 24 62
Cuts at: 2458 3435
Size: 977
Fragments arranged by size:
977 735 493 412 297 287 148 62
24
MspA1I CmG'CkG
Cuts at: 0 129 156 446 1143 1311 1557 2196 2302
Size: 129 27 290 697 168 246 639 106
Cuts at: 2302 2415 2458 2957 2977 3014 3159 3214 3320
Size: 113 43 499 20 37 145 55 106
Cuts at: 3320 3435
Size: 115
Fragments arranged by size:
697 639 499 290 246 168 145 129
115 113 106 106 55 43 37 27
20
NaeI GCC'GGC
Cuts at: 0 11 1119 3184 3435
Size: 11 1108 2065 251
Fragments arranged by size:
2065 1108 251 11
NarI GG'CG_CC
Cuts at: 0 26 102 173 3435
Size: 26 76 71 3262
Fragments arranged by size:
3262 76 71 26
NcoI C'CATG_G
Cuts at: 0 1415 3435
Size: 1415 2020
NgoMIV G'CCGG_C
Cuts at: 0 9 1117 3182 3435
Size: 9 1108 2065 253
Fragments arranged by size:
2065 1108 253 9
NheI G'CTAG_C
Cuts at: 0 2550 3435
Size: 2550 885
PasI CC'CwG_GG
Cuts at: 0 1537 3435
Size: 1537 1898
PflMI CCAn_nnn'nTGG
Cuts at: 0 791 3435
Size: 791 2644
PfoI T'CCnGG_A
Cuts at: 0 1712 1736 3435
Size: 1712 24 1699
Fragments arranged by size:
1712 1699 24
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 3435
Size: 685 32 2718
Fragments arranged by size:
2718 685 32
PpuMI rG'GwC_Cy
Cuts at: 0 579 1502 2058 3435
Size: 579 923 556 1377
Fragments arranged by size:
1377 923 579 556
PshAI GACnn'nnGTC
Cuts at: 0 937 3435
Size: 937 2498
PspOMI G'GGCC_C
Cuts at: 0 115 218 3435
Size: 115 103 3217
Fragments arranged by size:
3217 115 103
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1975 2007 3435
Size: 1975 32 1428
Fragments arranged by size:
1975 1428 32
PstI C_TGCA'G
Cuts at: 0 423 1148 1316 1976 3194 3435
Size: 423 725 168 660 1218 241
Fragments arranged by size:
1218 725 660 423 241 168
PvuII CAG'CTG
Cuts at: 0 1143 1557 2196 2302 2458 2957 3014 3435
Size: 1143 414 639 106 156 499 57 421
Fragments arranged by size:
1143 639 499 421 414 156 106 57
SacI G_AGCT'C
Cuts at: 0 674 2111 2468 3435
Size: 674 1437 357 967
Fragments arranged by size:
1437 967 674 357
SacII CC_GC'GG
Cuts at: 0 2978 3321 3435
Size: 2978 343 114
Fragments arranged by size:
2978 343 114
SalI G'TCGA_C
Cuts at: 0 241 3435
Size: 241 3194
SanDI GG'GwC_CC
Cuts at: 0 1502 3435
Size: 1502 1933
SbfI CC_TGCA'GG
Cuts at: 0 1976 3435
Size: 1976 1459
ScaI AGT'ACT
Cuts at: 0 2050 3037 3435
Size: 2050 987 398
Fragments arranged by size:
2050 987 398
SexAI A'CCwGG_T
Cuts at: 0 1031 2705 3435
Size: 1031 1674 730
Fragments arranged by size:
1674 1031 730
SfcI C'TryA_G
Cuts at: 0 183 419 1144 1312 1902 1929 1972 3190
Size: 183 236 725 168 590 27 43 1218
Cuts at: 3190 3435
Size: 245
Fragments arranged by size:
1218 725 590 245 236 183 168 43
27
SfoI GGC'GCC
Cuts at: 0 27 103 174 3435
Size: 27 76 71 3261
Fragments arranged by size:
3261 76 71 27
SmaI CCC'GGG
Cuts at: 0 2848 3435
Size: 2848 587
SmlI C'TyrA_G
Cuts at: 0 1690 3404 3435
Size: 1690 1714 31
Fragments arranged by size:
1714 1690 31
StyI C'CwwG_G
Cuts at: 0 491 1094 1415 2472 3435
Size: 491 603 321 1057 963
Fragments arranged by size:
1057 963 603 491 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 879 1869 2824 3435
Size: 879 990 955 611
Fragments arranged by size:
990 955 879 611
TatI w'GTAC_w
Cuts at: 0 479 621 1238 2048 3035 3435
Size: 479 142 617 810 987 400
Fragments arranged by size:
987 810 617 479 400 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1311 1343 2660 2692 3435
Size: 1311 32 1317 32 743
Fragments arranged by size:
1317 1311 743 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 874 3435
Size: 874 2561
XbaI T'CTAG_A
Cuts at: 0 1395 3435
Size: 1395 2040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2745 3175 3435
Size: 2745 430 260
Fragments arranged by size:
2745 430 260
XmaI C'CCGG_G
Cuts at: 0 2846 3435
Size: 2846 589
XmnI GAAnn'nnTTC
Cuts at: 0 310 700 1063 3435
Size: 310 390 363 2372
Fragments arranged by size:
2372 390 363 310
ZraI GAC'GTC
Cuts at: 0 110 1227 1302 3435
Size: 110 1117 75 2133
Fragments arranged by size:
2133 1117 110 75
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AhdI AleI
AloI AlwNI ApaI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII HpaI KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AlfI ApaLI ApoI AscI
AseI AsiSI BaeI BamHI BcgI BciVI BfrBI BglII
BplI BsaBI BsiWI BspEI BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EciI EcoRI EcoRV
FseI FspAI HindIII KpnI MluI NdeI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PsiI PspXI
PvuI RsrII SapI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XhoI