Restriction map for
pF1KA0560
(Linear) (Six-base) MAPSORT of: KIAA0560.seq Check: 5420 from: 1 to: 4455
KIAA0560.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4170 4455
Size: 4170 285
AccI GT'mk_AC
Cuts at: 0 476 4094 4455
Size: 476 3618 361
Fragments arranged by size:
3618 476 361
Acc65I G'GTAC_C
Cuts at: 0 2046 4455
Size: 2046 2409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3002 4455
Size: 3002 1453
AflIII A'CryG_T
Cuts at: 0 1815 3638 4100 4455
Size: 1815 1823 462 355
Fragments arranged by size:
1823 1815 462 355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3444 4455
Size: 3444 1011
AlwNI CAG_nnn'CTG
Cuts at: 0 10 1781 3201 4455
Size: 10 1771 1420 1254
Fragments arranged by size:
1771 1420 1254 10
ApaI G_GGCC'C
Cuts at: 0 2470 4455
Size: 2470 1985
ApaLI G'TGCA_C
Cuts at: 0 2096 4455
Size: 2096 2359
ApoI r'AATT_y
Cuts at: 0 202 409 583 916 1201 1241 1365 1439
Size: 202 207 174 333 285 40 124 74
Cuts at: 1439 1787 3008 3028 4455
Size: 348 1221 20 1427
Fragments arranged by size:
1427 1221 348 333 285 207 202 174
124 74 40 20
AseI AT'TA_AT
Cuts at: 0 938 3539 4455
Size: 938 2601 916
Fragments arranged by size:
2601 938 916
AvaI C'yCGr_G
Cuts at: 0 1082 1598 4455
Size: 1082 516 2857
Fragments arranged by size:
2857 1082 516
BamHI G'GATC_C
Cuts at: 0 621 1887 4455
Size: 621 1266 2568
Fragments arranged by size:
2568 1266 621
BanI G'GyrC_C
Cuts at: 0 2046 4455
Size: 2046 2409
BanII G_rGCy'C
Cuts at: 0 96 2342 2470 4455
Size: 96 2246 128 1985
Fragments arranged by size:
2246 1985 128 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 1187 1657 2219 2312 2972 3762 4455
Size: 1187 470 562 93 660 790 693
Fragments arranged by size:
1187 790 693 660 562 470 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2087 2121 3691 3725 4455
Size: 2087 34 1570 34 730
Fragments arranged by size:
2087 1570 730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1289 3930 4455
Size: 1289 2641 525
Fragments arranged by size:
2641 1289 525
BclI T'GATC_A
Cuts at: 0 450 4455
Size: 450 4005
BfrBI ATG'CAT
Cuts at: 0 4110 4455
Size: 4110 345
BglII A'GATC_T
Cuts at: 0 2953 3067 4455
Size: 2953 114 1388
Fragments arranged by size:
2953 1388 114
Bme1580I G_kGCm'C
Cuts at: 0 2100 2470 4455
Size: 2100 370 1985
Fragments arranged by size:
2100 1985 370
BmgBI CAC'GTC
Cuts at: 0 1816 4455
Size: 1816 2639
BmrI ACTGGGnnnn_n'
Cuts at: 0 66 99 4250 4310 4455
Size: 66 33 4151 60 145
Fragments arranged by size:
4151 145 66 60 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3940 4061 4455
Size: 3940 121 394
Fragments arranged by size:
3940 394 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1355 2029 2677 3223 3287 3935 4274 4325
Size: 1355 674 648 546 64 648 339 51
Cuts at: 4325 4455
Size: 130
Fragments arranged by size:
1355 674 648 648 546 339 130 64
51
Bpu10I CC'TnA_GC
Cuts at: 0 572 1399 4455
Size: 572 827 3056
Fragments arranged by size:
3056 827 572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3043 3389 4455
Size: 3043 346 1066
Fragments arranged by size:
3043 1066 346
BsaI GGTCTCn'nnnn_
Cuts at: 0 1734 4032 4360 4441 4455
Size: 1734 2298 328 81 14
Fragments arranged by size:
2298 1734 328 81 14
BsaBI GATnn'nnATC
Cuts at: 0 884 4455
Size: 884 3571
BsaWI w'CCGG_w
Cuts at: 0 1733 4455
Size: 1733 2722
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4323 4353 4455
Size: 4323 30 102
Fragments arranged by size:
4323 102 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 669 1752 1871 1986 2921 4455
Size: 669 1083 119 115 935 1534
Fragments arranged by size:
1534 1083 935 669 119 115
BseYI C'CCAG_C
Cuts at: 0 896 1532 2828 4455
Size: 896 636 1296 1627
Fragments arranged by size:
1627 1296 896 636
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1628 2044 3499 4382 4455
Size: 1628 416 1455 883 73
Fragments arranged by size:
1628 1455 883 416 73
BsiHKAI G_wGCw'C
Cuts at: 0 2100 4455
Size: 2100 2355
BsiWI C'GTAC_G
Cuts at: 0 1698 4455
Size: 1698 2757
BsmI GAATG_Cn'
Cuts at: 0 2444 4455
Size: 2444 2011
Bsp1286I G_dGCh'C
Cuts at: 0 96 2100 2342 2470 4455
Size: 96 2004 242 128 1985
Fragments arranged by size:
2004 1985 242 128 96
BspHI T'CATG_A
Cuts at: 0 420 1230 1386 4047 4455
Size: 420 810 156 2661 408
Fragments arranged by size:
2661 810 420 408 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1403 1746 2669 4170 4455
Size: 1403 343 923 1501 285
Fragments arranged by size:
1501 1403 923 343 285
BsrDI GCAATG_nn'
Cuts at: 0 453 2869 4455
Size: 453 2416 1586
Fragments arranged by size:
2416 1586 453
BsrFI r'CCGG_y
Cuts at: 0 4401 4455
Size: 4401 54
BsrGI T'GTAC_A
Cuts at: 0 3635 4103 4455
Size: 3635 468 352
Fragments arranged by size:
3635 468 352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 10 3486 4455
Size: 10 3476 969
Fragments arranged by size:
3476 969 10
BstBI TT'CG_AA
Cuts at: 0 2107 3051 4455
Size: 2107 944 1404
Fragments arranged by size:
2107 1404 944
BstEII G'GTnAC_C
Cuts at: 0 3652 4455
Size: 3652 803
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3840 4455
Size: 3840 615
BstYI r'GATC_y
Cuts at: 0 621 1887 2953 3067 4455
Size: 621 1266 1066 114 1388
Fragments arranged by size:
1388 1266 1066 621 114
BstZ17I GTA'TAC
Cuts at: 0 4095 4455
Size: 4095 360
Bsu36I CC'TnA_GG
Cuts at: 0 248 3242 4421 4455
Size: 248 2994 1179 34
Fragments arranged by size:
2994 1179 248 34
BtgI C'CryG_G
Cuts at: 0 1480 4455
Size: 1480 2975
BtsI GCAGTG_nn'
Cuts at: 0 3845 4455
Size: 3845 610
DraI TTT'AAA
Cuts at: 0 369 2171 2946 4455
Size: 369 1802 775 1509
Fragments arranged by size:
1802 1509 775 369
DraIII CAC_nnn'GTG
Cuts at: 0 1137 4455
Size: 1137 3318
EaeI y'GGCC_r
Cuts at: 0 882 1565 1591 3693 3875 4455
Size: 882 683 26 2102 182 580
Fragments arranged by size:
2102 882 683 580 182 26
EarI CTCTTCn'nnn_
Cuts at: 0 869 1172 1358 1388 1420 2645 2682 2828
Size: 869 303 186 30 32 1225 37 146
Cuts at: 2828 3179 3230 3361 3931 4169 4217 4439 4455
Size: 351 51 131 570 238 48 222 16
Fragments arranged by size:
1225 869 570 351 303 238 222 186
146 131 51 48 37 32 30 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1355 2029 2677 3002 3223 3287 3935 4274
Size: 1355 674 648 325 221 64 648 339
Cuts at: 4274 4325 4455
Size: 51 130
Fragments arranged by size:
1355 674 648 648 339 325 221 130
64 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2477 4455
Size: 2477 1978
EcoO109I rG'GnC_Cy
Cuts at: 0 783 2085 2362 2571 4455
Size: 783 1302 277 209 1884
Fragments arranged by size:
1884 1302 783 277 209
EcoRI G'AATT_C
Cuts at: 0 3028 4455
Size: 3028 1427
EcoRV GAT'ATC
Cuts at: 0 2067 4455
Size: 2067 2388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1222 1363 4455
Size: 1222 141 3092
Fragments arranged by size:
3092 1222 141
FspI TGC'GCA
Cuts at: 0 14 4455
Size: 14 4441
HaeII r_GCGC'y
Cuts at: 0 804 2622 4455
Size: 804 1818 1833
Fragments arranged by size:
1833 1818 804
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 474 506 1628 1660 3102 3134 3270 3302
Size: 474 32 1122 32 1442 32 136 32
Cuts at: 3302 3890 3922 4027 4059 4113 4145 4417 4449
Size: 588 32 105 32 54 32 272 32
Cuts at: 4449 4455
Size: 6
Fragments arranged by size:
1442 1122 588 474 272 136 105 54
32 32 32 32 32 32 32 32
6
HincII GTy'rAC
Cuts at: 0 3393 3796 4455
Size: 3393 403 659
Fragments arranged by size:
3393 659 403
HindIII A'AGCT_T
Cuts at: 0 3423 4455
Size: 3423 1032
KpnI G_GTAC'C
Cuts at: 0 2050 4455
Size: 2050 2405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 2537 3357 3409 4024 4285 4455
Size: 1306 1231 820 52 615 261 170
Fragments arranged by size:
1306 1231 820 615 261 170 52
MscI TGG'CCA
Cuts at: 0 884 1567 1593 3695 3877 4455
Size: 884 683 26 2102 182 578
Fragments arranged by size:
2102 884 683 578 182 26
MslI CAynn'nnrTG
Cuts at: 0 746 3178 4105 4455
Size: 746 2432 927 350
Fragments arranged by size:
2432 927 746 350
MspA1I CmG'CkG
Cuts at: 0 537 4274 4455
Size: 537 3737 181
Fragments arranged by size:
3737 537 181
NcoI C'CATG_G
Cuts at: 0 1480 4455
Size: 1480 2975
NruI TCG'CGA
Cuts at: 0 3713 4455
Size: 3713 742
NsiI A_TGCA'T
Cuts at: 0 4112 4455
Size: 4112 343
NspI r_CATG'y
Cuts at: 0 83 912 1800 3642 4104 4110 4455
Size: 83 829 888 1842 462 6 345
Fragments arranged by size:
1842 888 829 462 345 83 6
PciI A'CATG_T
Cuts at: 0 3638 4100 4455
Size: 3638 462 355
Fragments arranged by size:
3638 462 355
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 903 1137 3751 4455
Size: 837 66 234 2614 704
Fragments arranged by size:
2614 837 704 234 66
PfoI T'CCnGG_A
Cuts at: 0 818 2763 4455
Size: 818 1945 1692
Fragments arranged by size:
1945 1692 818
PpuMI rG'GwC_Cy
Cuts at: 0 2085 2362 4455
Size: 2085 277 2093
Fragments arranged by size:
2093 2085 277
PsiI TTA'TAA
Cuts at: 0 2375 4455
Size: 2375 2080
PspOMI G'GGCC_C
Cuts at: 0 2466 4455
Size: 2466 1989
PstI C_TGCA'G
Cuts at: 0 2796 4455
Size: 2796 1659
PvuII CAG'CTG
Cuts at: 0 537 4274 4455
Size: 537 3737 181
Fragments arranged by size:
3737 537 181
SanDI GG'GwC_CC
Cuts at: 0 2085 4455
Size: 2085 2370
SapI GCTCTTCn'nnn_
Cuts at: 0 1358 2645 4455
Size: 1358 1287 1810
Fragments arranged by size:
1810 1358 1287
ScaI AGT'ACT
Cuts at: 0 1327 3334 4455
Size: 1327 2007 1121
Fragments arranged by size:
2007 1327 1121
SexAI A'CCwGG_T
Cuts at: 0 1133 2472 2813 4455
Size: 1133 1339 341 1642
Fragments arranged by size:
1642 1339 1133 341
SfcI C'TryA_G
Cuts at: 0 1009 2207 2792 3232 4455
Size: 1009 1198 585 440 1223
Fragments arranged by size:
1223 1198 1009 585 440
SmlI C'TyrA_G
Cuts at: 0 3022 3404 4455
Size: 3022 382 1051
Fragments arranged by size:
3022 1051 382
SphI G_CATG'C
Cuts at: 0 1800 4455
Size: 1800 2655
SspI AAT'ATT
Cuts at: 0 1311 4455
Size: 1311 3144
StuI AGG'CCT
Cuts at: 0 265 4455
Size: 265 4190
StyI C'CwwG_G
Cuts at: 0 1091 1480 4455
Size: 1091 389 2975
Fragments arranged by size:
2975 1091 389
TatI w'GTAC_w
Cuts at: 0 495 1325 2866 3115 3332 3499 3635 4053
Size: 495 830 1541 249 217 167 136 418
Cuts at: 4053 4103 4455
Size: 50 352
Fragments arranged by size:
1541 830 495 418 352 249 217 167
136 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 659 691 4323 4355 4455
Size: 659 32 3632 32 100
Fragments arranged by size:
3632 659 100 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3145 4455
Size: 3145 1310
XbaI T'CTAG_A
Cuts at: 0 777 1209 3883 4455
Size: 777 432 2674 572
Fragments arranged by size:
2674 777 572 432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 27 4377 4455
Size: 27 4350 78
Fragments arranged by size:
4350 78 27
XmnI GAAnn'nnTTC
Cuts at: 0 1002 1205 1664 2671 3047 3551 4455
Size: 1002 203 459 1007 376 504 904
Fragments arranged by size:
1007 1002 904 504 459 376 203
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI DraI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI
MspA1I NcoI NruI NsiI NspI PciI PflMI PfoI
PpuMI PsiI PspOMI PstI PvuII SanDI SapI ScaI
SexAI SfcI SmlI SphI SspI StuI StyI TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
AscI AsiSI AvrII BaeI BbeI BbvCI BglI BlpI
BmtI BsaAI BsaHI BsiEI BsmBI BspEI BsrBI BssHII
BssSI BtgZI ClaI CspCI DrdI EagI EciI EcoICRI
FseI FspAI HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI PacI PasI PmeI PmlI
PpiI PshAI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI TaqII XhoI XmaI ZraI