Restriction map for
pF1KA0562
(Linear) (Six-base) MAPSORT of: KIAA0562.seq Check: 5387 from: 1 to: 2775
KIAA0562.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 1076 2775
Size: 1076 1699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1006 1721 2162 2237 2538 2775
Size: 39 967 715 441 75 301 237
Fragments arranged by size:
967 715 441 301 237 75 39
AflIII A'CryG_T
Cuts at: 0 290 1904 2775
Size: 290 1614 871
Fragments arranged by size:
1614 871 290
AgeI A'CCGG_T
Cuts at: 0 2528 2775
Size: 2528 247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 200 854 2775
Size: 200 654 1921
Fragments arranged by size:
1921 654 200
AlwNI CAG_nnn'CTG
Cuts at: 0 2032 2212 2775
Size: 2032 180 563
Fragments arranged by size:
2032 563 180
ApaI G_GGCC'C
Cuts at: 0 2596 2775
Size: 2596 179
ApoI r'AATT_y
Cuts at: 0 1647 2132 2775
Size: 1647 485 643
Fragments arranged by size:
1647 643 485
AvaI C'yCGr_G
Cuts at: 0 62 329 494 1806 2775
Size: 62 267 165 1312 969
Fragments arranged by size:
1312 969 267 165 62
BanII G_rGCy'C
Cuts at: 0 71 1256 2596 2752 2775
Size: 71 1185 1340 156 23
Fragments arranged by size:
1340 1185 156 71 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 285 797 903 2775
Size: 53 232 512 106 1872
Fragments arranged by size:
1872 512 232 106 53
BbvCI CC'TCA_GC
Cuts at: 0 1061 1322 2775
Size: 1061 261 1453
Fragments arranged by size:
1453 1061 261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 523 557 2598 2632 2775
Size: 523 34 2041 34 143
Fragments arranged by size:
2041 523 143 34 34
BclI T'GATC_A
Cuts at: 0 1517 2775
Size: 1517 1258
BglI GCCn_nnn'nGGC
Cuts at: 0 2600 2775
Size: 2600 175
BlpI GC'TnA_GC
Cuts at: 0 2670 2775
Size: 2670 105
Bme1580I G_kGCm'C
Cuts at: 0 63 527 1798 1851 2046 2596 2775
Size: 63 464 1271 53 195 550 179
Fragments arranged by size:
1271 550 464 195 179 63 53
BmgBI CAC'GTC
Cuts at: 0 1340 2775
Size: 1340 1435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 901 1631 1873 1931 1954 2582 2736
Size: 880 21 730 242 58 23 628 154
Cuts at: 2736 2775
Size: 39
Fragments arranged by size:
880 730 628 242 154 58 39 23
21
Bpu10I CC'TnA_GC
Cuts at: 0 1061 1322 2775
Size: 1061 261 1453
Fragments arranged by size:
1453 1061 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1293 2775
Size: 1293 1482
BsaI GGTCTCn'nnnn_
Cuts at: 0 449 2775
Size: 449 2326
BsaAI yAC'GTr
Cuts at: 0 291 2775
Size: 291 2484
BsaBI GATnn'nnATC
Cuts at: 0 1103 2775
Size: 1103 1672
BsaWI w'CCGG_w
Cuts at: 0 2511 2528 2775
Size: 2511 17 247
Fragments arranged by size:
2511 247 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1041 1071 2775
Size: 1041 30 1704
Fragments arranged by size:
1704 1041 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1049 2775
Size: 1049 1726
BseYI C'CCAG_C
Cuts at: 0 2595 2775
Size: 2595 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1735 2589 2775
Size: 850 885 854 186
Fragments arranged by size:
885 854 850 186
BsiHKAI G_wGCw'C
Cuts at: 0 71 2243 2393 2752 2775
Size: 71 2172 150 359 23
Fragments arranged by size:
2172 359 150 71 23
BsiWI C'GTAC_G
Cuts at: 0 557 2762 2775
Size: 557 2205 13
Fragments arranged by size:
2205 557 13
BsmI GAATG_Cn'
Cuts at: 0 1065 2775
Size: 1065 1710
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1873 2775
Size: 1873 902
Bsp1286I G_dGCh'C
Cuts at: 0 63 71 527 1256 1798 1851 2046 2243
Size: 63 8 456 729 542 53 195 197
Cuts at: 2243 2393 2596 2752 2775
Size: 150 203 156 23
Fragments arranged by size:
729 542 456 203 197 195 156 150
63 53 23 8
BspHI T'CATG_A
Cuts at: 0 71 2544 2775
Size: 71 2473 231
Fragments arranged by size:
2473 231 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2624 2775
Size: 2624 151
BsrBI CCG'CTC
Cuts at: 0 270 1143 2775
Size: 270 873 1632
Fragments arranged by size:
1632 873 270
BsrDI GCAATG_nn'
Cuts at: 0 716 1757 2775
Size: 716 1041 1018
Fragments arranged by size:
1041 1018 716
BsrFI r'CCGG_y
Cuts at: 0 2528 2599 2775
Size: 2528 71 176
Fragments arranged by size:
2528 176 71
BsrGI T'GTAC_A
Cuts at: 0 1907 2775
Size: 1907 868
BstAPI GCAn_nnn'nTGC
Cuts at: 0 944 1366 2212 2775
Size: 944 422 846 563
Fragments arranged by size:
944 846 563 422
BstBI TT'CG_AA
Cuts at: 0 276 1890 2775
Size: 276 1614 885
Fragments arranged by size:
1614 885 276
BstEII G'GTnAC_C
Cuts at: 0 104 2775
Size: 104 2671
BstXI CCAn_nnnn'nTGG
Cuts at: 0 13 520 2775
Size: 13 507 2255
Fragments arranged by size:
2255 507 13
BstYI r'GATC_y
Cuts at: 0 618 1098 2307 2717 2775
Size: 618 480 1209 410 58
Fragments arranged by size:
1209 618 480 410 58
BtgI C'CryG_G
Cuts at: 0 779 2775
Size: 779 1996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1232 2775
Size: 1232 1543
BtsI GCAGTG_nn'
Cuts at: 0 1187 1360 2352 2451 2650 2775
Size: 1187 173 992 99 199 125
Fragments arranged by size:
1187 992 199 173 125 99
ClaI AT'CG_AT
Cuts at: 0 1295 2775
Size: 1295 1480
DraI TTT'AAA
Cuts at: 0 1421 1674 2775
Size: 1421 253 1101
Fragments arranged by size:
1421 1101 253
DraIII CAC_nnn'GTG
Cuts at: 0 1575 2775
Size: 1575 1200
EaeI y'GGCC_r
Cuts at: 0 2678 2775
Size: 2678 97
EarI CTCTTCn'nnn_
Cuts at: 0 1154 1452 1740 2459 2561 2775
Size: 1154 298 288 719 102 214
Fragments arranged by size:
1154 719 298 288 214 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2750 2775
Size: 2750 25
EcoICRI GAG'CTC
Cuts at: 0 69 2750 2775
Size: 69 2681 25
Fragments arranged by size:
2681 69 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 880 901 1006 1631 1721 1873 1931
Size: 39 841 21 105 625 90 152 58
Cuts at: 1931 1954 2162 2237 2538 2582 2736 2775
Size: 23 208 75 301 44 154 39
Fragments arranged by size:
841 625 301 208 154 152 105 90
75 58 44 39 39 23 21
EcoO109I rG'GnC_Cy
Cuts at: 0 537 2643 2689 2775
Size: 537 2106 46 86
Fragments arranged by size:
2106 537 86 46
EcoRV GAT'ATC
Cuts at: 0 2375 2775
Size: 2375 400
FspI TGC'GCA
Cuts at: 0 2620 2775
Size: 2620 155
HaeII r_GCGC'y
Cuts at: 0 747 2775
Size: 747 2028
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 565 597 1427 1459 2364 2396
Size: 8 32 525 32 830 32 905 32
Cuts at: 2396 2775
Size: 379
Fragments arranged by size:
905 830 525 379 32 32 32 32
8
HincII GTy'rAC
Cuts at: 0 402 2775
Size: 402 2373
HindIII A'AGCT_T
Cuts at: 0 234 2099 2775
Size: 234 1865 676
Fragments arranged by size:
1865 676 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1380 2775
Size: 1380 1395
MslI CAynn'nnrTG
Cuts at: 0 1297 2775
Size: 1297 1478
MspA1I CmG'CkG
Cuts at: 0 858 1642 2115 2674 2775
Size: 858 784 473 559 101
Fragments arranged by size:
858 784 559 473 101
NaeI GCC'GGC
Cuts at: 0 2601 2775
Size: 2601 174
NgoMIV G'CCGG_C
Cuts at: 0 2599 2775
Size: 2599 176
NspI r_CATG'y
Cuts at: 0 1239 1908 2775
Size: 1239 669 867
Fragments arranged by size:
1239 867 669
PciI A'CATG_T
Cuts at: 0 1904 2775
Size: 1904 871
PfoI T'CCnGG_A
Cuts at: 0 875 1931 2231 2775
Size: 875 1056 300 544
Fragments arranged by size:
1056 875 544 300
PpuMI rG'GwC_Cy
Cuts at: 0 537 2775
Size: 537 2238
PspOMI G'GGCC_C
Cuts at: 0 2592 2775
Size: 2592 183
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1963 1995 2775
Size: 1963 32 780
Fragments arranged by size:
1963 780 32
PstI C_TGCA'G
Cuts at: 0 179 465 1074 2639 2657 2775
Size: 179 286 609 1565 18 118
Fragments arranged by size:
1565 609 286 179 118 18
PvuII CAG'CTG
Cuts at: 0 858 1642 2115 2674 2775
Size: 858 784 473 559 101
Fragments arranged by size:
858 784 559 473 101
SacI G_AGCT'C
Cuts at: 0 71 2752 2775
Size: 71 2681 23
Fragments arranged by size:
2681 71 23
SapI GCTCTTCn'nnn_
Cuts at: 0 1452 2459 2775
Size: 1452 1007 316
Fragments arranged by size:
1452 1007 316
SexAI A'CCwGG_T
Cuts at: 0 1709 2775
Size: 1709 1066
SfcI C'TryA_G
Cuts at: 0 175 461 1070 2024 2060 2635 2653 2775
Size: 175 286 609 954 36 575 18 122
Fragments arranged by size:
954 609 575 286 175 122 36 18
SmaI CCC'GGG
Cuts at: 0 64 331 2775
Size: 64 267 2444
Fragments arranged by size:
2444 267 64
SmlI C'TyrA_G
Cuts at: 0 494 1272 2775
Size: 494 778 1503
Fragments arranged by size:
1503 778 494
SspI AAT'ATT
Cuts at: 0 247 2775
Size: 247 2528
StuI AGG'CCT
Cuts at: 0 1270 1327 2775
Size: 1270 57 1448
Fragments arranged by size:
1448 1270 57
StyI C'CwwG_G
Cuts at: 0 519 747 806 1313 2693 2775
Size: 519 228 59 507 1380 82
Fragments arranged by size:
1380 519 507 228 82 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 86 2775
Size: 86 2689
TatI w'GTAC_w
Cuts at: 0 1907 2775
Size: 1907 868
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1909 1941 2775
Size: 1909 32 834
Fragments arranged by size:
1909 834 32
Tth111I GACn'n_nGTC
Cuts at: 0 1341 2775
Size: 1341 1434
XbaI T'CTAG_A
Cuts at: 0 546 2247 2775
Size: 546 1701 528
Fragments arranged by size:
1701 546 528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1299 2775
Size: 1299 1476
XhoI C'TCGA_G
Cuts at: 0 494 2775
Size: 494 2281
XmaI C'CCGG_G
Cuts at: 0 62 329 2775
Size: 62 267 2446
Fragments arranged by size:
2446 267 62
Enzymes that do cut:
AccI AcuI AflIII AgeI AlfI AlwNI ApaI ApoI
AvaI BanII BbsI BbvCI BcgI BclI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FspI HaeII Hin4I HincII HindIII MmeI
MslI MspA1I NaeI NgoMIV NspI PciI PfoI PpuMI
PspOMI PsrI PstI PvuII SacI SapI SexAI SfcI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BciVI BfrBI BglII BmrI BmtI BplI
BsaHI BsiEI BspEI BssHII BssSI BstZ17I Bsu36I CspCI
DrdI EagI EcoNI EcoRI FalI FseI FspAI HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI XmnI ZraI