Restriction map for
pF1KA0571
(Linear) (Six-base) MAPSORT of: KIAA0571.seq Check: 340 from: 1 to: 1914
KIAA0571.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1279 1914
Size: 1279 635
AccI GT'mk_AC
Cuts at: 0 818 1310 1914
Size: 818 492 604
Fragments arranged by size:
818 604 492
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 1914
Size: 1250 664
AhdI GACnn_n'nnGTC
Cuts at: 0 595 1914
Size: 595 1319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 110 1914
Size: 110 1804
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 1914
Size: 28 32 1854
Fragments arranged by size:
1854 32 28
AlwNI CAG_nnn'CTG
Cuts at: 0 1660 1845 1914
Size: 1660 185 69
Fragments arranged by size:
1660 185 69
ApaI G_GGCC'C
Cuts at: 0 1335 1914
Size: 1335 579
ApaLI G'TGCA_C
Cuts at: 0 1378 1914
Size: 1378 536
ApoI r'AATT_y
Cuts at: 0 664 1914
Size: 664 1250
AvrII C'CTAG_G
Cuts at: 0 865 1914
Size: 865 1049
BanI G'GyrC_C
Cuts at: 0 526 1903 1914
Size: 526 1377 11
Fragments arranged by size:
1377 526 11
BanII G_rGCy'C
Cuts at: 0 320 1327 1335 1753 1914
Size: 320 1007 8 418 161
Fragments arranged by size:
1007 418 320 161 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1238 1840 1914
Size: 1238 602 74
Fragments arranged by size:
1238 602 74
BbvCI CC'TCA_GC
Cuts at: 0 248 282 360 992 1914
Size: 248 34 78 632 922
Fragments arranged by size:
922 632 248 78 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 783 817 1011 1045 1914
Size: 783 34 194 34 869
Fragments arranged by size:
869 783 194 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 651 734 1914
Size: 651 83 1180
Fragments arranged by size:
1180 651 83
BglII A'GATC_T
Cuts at: 0 227 1024 1914
Size: 227 797 890
Fragments arranged by size:
890 797 227
BlpI GC'TnA_GC
Cuts at: 0 314 319 1914
Size: 314 5 1595
Fragments arranged by size:
1595 314 5
Bme1580I G_kGCm'C
Cuts at: 0 529 1247 1335 1382 1914
Size: 529 718 88 47 532
Fragments arranged by size:
718 532 529 88 47
BmgBI CAC'GTC
Cuts at: 0 1875 1914
Size: 1875 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 1563 1648 1670 1914
Size: 1563 85 22 244
Fragments arranged by size:
1563 244 85 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 360 1542 1914
Size: 293 67 1182 372
Fragments arranged by size:
1182 372 293 67
Bpu10I CC'TnA_GC
Cuts at: 0 248 282 360 992 1914
Size: 248 34 78 632 922
Fragments arranged by size:
922 632 248 78 34
BsaI GGTCTCn'nnnn_
Cuts at: 0 993 1099 1914
Size: 993 106 815
Fragments arranged by size:
993 815 106
BsaHI Gr'CG_yC
Cuts at: 0 775 1914
Size: 775 1139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 1546 1576 1914
Size: 28 30 1488 30 338
Fragments arranged by size:
1488 338 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 564 1554 1914
Size: 265 299 990 360
Fragments arranged by size:
990 360 299 265
BseYI C'CCAG_C
Cuts at: 0 778 1390 1914
Size: 778 612 524
Fragments arranged by size:
778 612 524
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1160 1914
Size: 813 347 754
Fragments arranged by size:
813 754 347
BsiEI CG_ry'CG
Cuts at: 0 1213 1914
Size: 1213 701
BsiHKAI G_wGCw'C
Cuts at: 0 259 320 1382 1711 1753 1914
Size: 259 61 1062 329 42 161
Fragments arranged by size:
1062 329 259 161 61 42
Bsp1286I G_dGCh'C
Cuts at: 0 259 320 529 1247 1327 1335 1382 1711
Size: 259 61 209 718 80 8 47 329
Cuts at: 1711 1753 1914
Size: 42 161
Fragments arranged by size:
718 329 259 209 161 80 61 47
42 8
BspHI T'CATG_A
Cuts at: 0 545 1914
Size: 545 1369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 1279 1914
Size: 78 1201 635
Fragments arranged by size:
1201 635 78
BsrBI CCG'CTC
Cuts at: 0 5 302 1914
Size: 5 297 1612
Fragments arranged by size:
1612 297 5
BsrDI GCAATG_nn'
Cuts at: 0 591 1073 1914
Size: 591 482 841
Fragments arranged by size:
841 591 482
BsrFI r'CCGG_y
Cuts at: 0 286 650 1145 1711 1914
Size: 286 364 495 566 203
Fragments arranged by size:
566 495 364 286 203
BsrGI T'GTAC_A
Cuts at: 0 564 761 1914
Size: 564 197 1153
Fragments arranged by size:
1153 564 197
BstAPI GCAn_nnn'nTGC
Cuts at: 0 499 508 1914
Size: 499 9 1406
Fragments arranged by size:
1406 499 9
BstEII G'GTnAC_C
Cuts at: 0 7 1914
Size: 7 1907
BstXI CCAn_nnnn'nTGG
Cuts at: 0 232 586 906 1272 1914
Size: 232 354 320 366 642
Fragments arranged by size:
642 366 354 320 232
BstYI r'GATC_y
Cuts at: 0 227 860 1024 1914
Size: 227 633 164 890
Fragments arranged by size:
890 633 227 164
Bsu36I CC'TnA_GG
Cuts at: 0 452 1474 1914
Size: 452 1022 440
Fragments arranged by size:
1022 452 440
BtgI C'CryG_G
Cuts at: 0 621 708 1277 1914
Size: 621 87 569 637
Fragments arranged by size:
637 621 569 87
ClaI AT'CG_AT
Cuts at: 0 1493 1914
Size: 1493 421
DraIII CAC_nnn'GTG
Cuts at: 0 791 1126 1914
Size: 791 335 788
Fragments arranged by size:
791 788 335
EaeI y'GGCC_r
Cuts at: 0 711 836 908 1274 1914
Size: 711 125 72 366 640
Fragments arranged by size:
711 640 366 125 72
EarI CTCTTCn'nnn_
Cuts at: 0 185 309 483 1622 1699 1914
Size: 185 124 174 1139 77 215
Fragments arranged by size:
1139 215 185 174 124 77
EcoICRI GAG'CTC
Cuts at: 0 318 1751 1914
Size: 318 1433 163
Fragments arranged by size:
1433 318 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 360 1250 1542 1914
Size: 293 67 890 292 372
Fragments arranged by size:
890 372 293 292 67
EcoO109I rG'GnC_Cy
Cuts at: 0 807 1331 1387 1842 1914
Size: 807 524 56 455 72
Fragments arranged by size:
807 524 455 72 56
EcoRI G'AATT_C
Cuts at: 0 664 1914
Size: 664 1250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 581 597 613 629 861 893
Size: 340 32 209 16 16 16 232 32
Cuts at: 893 992 1024 1914
Size: 99 32 890
Fragments arranged by size:
890 340 232 209 99 32 32 32
16 16 16
HincII GTy'rAC
Cuts at: 0 600 1425 1914
Size: 600 825 489
Fragments arranged by size:
825 600 489
MscI TGG'CCA
Cuts at: 0 713 910 1276 1914
Size: 713 197 366 638
Fragments arranged by size:
713 638 366 197
MslI CAynn'nnrTG
Cuts at: 0 904 978 1318 1914
Size: 904 74 340 596
Fragments arranged by size:
904 596 340 74
MspA1I CmG'CkG
Cuts at: 0 313 562 1914
Size: 313 249 1352
Fragments arranged by size:
1352 313 249
NaeI GCC'GGC
Cuts at: 0 652 1914
Size: 652 1262
NcoI C'CATG_G
Cuts at: 0 621 708 1277 1914
Size: 621 87 569 637
Fragments arranged by size:
637 621 569 87
NgoMIV G'CCGG_C
Cuts at: 0 650 1914
Size: 650 1264
NspI r_CATG'y
Cuts at: 0 426 1914
Size: 426 1488
PasI CC'CwG_GG
Cuts at: 0 579 1327 1914
Size: 579 748 587
Fragments arranged by size:
748 587 579
PflMI CCAn_nnn'nTGG
Cuts at: 0 1126 1529 1914
Size: 1126 403 385
Fragments arranged by size:
1126 403 385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 1914
Size: 28 32 1854
Fragments arranged by size:
1854 32 28
PpuMI rG'GwC_Cy
Cuts at: 0 807 1914
Size: 807 1107
PspOMI G'GGCC_C
Cuts at: 0 1331 1914
Size: 1331 583
PstI C_TGCA'G
Cuts at: 0 128 1850 1914
Size: 128 1722 64
Fragments arranged by size:
1722 128 64
PvuI CG_AT'CG
Cuts at: 0 1213 1914
Size: 1213 701
PvuII CAG'CTG
Cuts at: 0 313 562 1914
Size: 313 249 1352
Fragments arranged by size:
1352 313 249
SacI G_AGCT'C
Cuts at: 0 320 1753 1914
Size: 320 1433 161
Fragments arranged by size:
1433 320 161
SapI GCTCTTCn'nnn_
Cuts at: 0 309 483 1699 1914
Size: 309 174 1216 215
Fragments arranged by size:
1216 309 215 174
ScaI AGT'ACT
Cuts at: 0 1573 1914
Size: 1573 341
SexAI A'CCwGG_T
Cuts at: 0 173 1914
Size: 173 1741
SfcI C'TryA_G
Cuts at: 0 124 630 1846 1914
Size: 124 506 1216 68
Fragments arranged by size:
1216 506 124 68
StuI AGG'CCT
Cuts at: 0 101 1914
Size: 101 1813
StyI C'CwwG_G
Cuts at: 0 621 708 719 865 1277 1898 1914
Size: 621 87 11 146 412 621 16
Fragments arranged by size:
621 621 412 146 87 16 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1606 1914
Size: 1606 308
TatI w'GTAC_w
Cuts at: 0 564 761 1571 1914
Size: 564 197 810 343
Fragments arranged by size:
810 564 343 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1674 1706 1914
Size: 1674 32 208
Fragments arranged by size:
1674 208 32
XmnI GAAnn'nnTTC
Cuts at: 0 276 1914
Size: 276 1638
Enzymes that do cut:
AarI AccI AcuI AhdI AlfI AloI AlwNI ApaI
ApaLI ApoI AvrII BanI BanII BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI ClaI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PpiI PpuMI PspOMI PstI PvuI PvuII SacI SapI
ScaI SexAI SfcI StuI StyI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AscI AseI AsiSI AvaI BaeI BamHI BbeI BciVI
BclI BfrBI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BssHII BssSI BstBI BstZ17I
BtgZI BtsI CspCI DraI DrdI EagI EciI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI