(Linear) (Six-base) MAPSORT of: KIAA0573.seq  Check: 3686  from: 1  to: 1218



KIAA0573.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AccI GT'mk_AC

Cuts at:      0    1189    1218 
   Size:       1189      29
 
ApoI r'AATT_y

Cuts at:      0     122     153     232     437     684     907    1075    1218 
   Size:        122      31      79     205     247     223     168     143

  Fragments arranged by size: 

                 247     223     205     168     143     122      79      31
            
 
AseI AT'TA_AT

Cuts at:      0     859    1015    1218 
   Size:        859     156     203

  Fragments arranged by size: 

                 859     203     156
 
BanII G_rGCy'C

Cuts at:      0    1156    1218 
   Size:       1156      62
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       6    1218 
   Size:          6    1212
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     534     568    1218 
   Size:        534      34     650

  Fragments arranged by size: 

                 650     534      34
 
BclI T'GATC_A

Cuts at:      0     276    1218 
   Size:        276     942
 
BfrBI ATG'CAT

Cuts at:      0       3    1218 
   Size:          3    1215
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1169    1218 
   Size:       1169      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     926    1218 
   Size:        926     292
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1015    1218 
   Size:       1015     203
 
BsaBI GATnn'nnATC

Cuts at:      0     275    1218 
   Size:        275     943
 
BsaWI w'CCGG_w

Cuts at:      0     600     654    1218 
   Size:        600      54     564

  Fragments arranged by size: 

                 600     564      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     910    1051    1218 
   Size:        910     141     167

  Fragments arranged by size: 

                 910     167     141
 
BsiHKAI G_wGCw'C

Cuts at:      0    1156    1218 
   Size:       1156      62
 
BsmI GAATG_Cn'

Cuts at:      0     645    1218 
   Size:        645     573
 
Bsp1286I G_dGCh'C

Cuts at:      0    1156    1218 
   Size:       1156      62
 
BspEI T'CCGG_A

Cuts at:      0     654    1218 
   Size:        654     564
 
BstZ17I GTA'TAC

Cuts at:      0    1190    1218 
   Size:       1190      28
 
DraI TTT'AAA

Cuts at:      0     128    1218 
   Size:        128    1090
 
DraIII CAC_nnn'GTG

Cuts at:      0    1177    1218 
   Size:       1177      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     352     926    1218 
   Size:        352     574     292

  Fragments arranged by size: 

                 574     352     292
 
EcoICRI GAG'CTC

Cuts at:      0    1154    1218 
   Size:       1154      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     926    1218 
   Size:        926     292
 
EcoRI G'AATT_C

Cuts at:      0    1075    1218 
   Size:       1075     143
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     268     300    1218 
   Size:        177      32      59      32     918

  Fragments arranged by size: 

                 918     177      59      32      32
 
HindIII A'AGCT_T

Cuts at:      0     209    1218 
   Size:        209    1009
 
MfeI C'AATT_G

Cuts at:      0     702    1218 
   Size:        702     516
 
MslI CAynn'nnrTG

Cuts at:      0     986    1218 
   Size:        986     232
 
NdeI CA'TA_TG

Cuts at:      0     983    1218 
   Size:        983     235
 
NsiI A_TGCA'T

Cuts at:      0       5    1218 
   Size:          5    1213
 
NspI r_CATG'y

Cuts at:      0     895    1218 
   Size:        895     323
 
PfoI T'CCnGG_A

Cuts at:      0    1020    1218 
   Size:       1020     198
 
PshAI GACnn'nnGTC

Cuts at:      0     170    1218 
   Size:        170    1048
 
SacI G_AGCT'C

Cuts at:      0    1156    1218 
   Size:       1156      62
 
SfcI C'TryA_G

Cuts at:      0     522    1218 
   Size:        522     696
 
SmlI C'TyrA_G

Cuts at:      0    1030    1218 
   Size:       1030     188
 
SspI AAT'ATT

Cuts at:      0     549     833    1218 
   Size:        549     284     385

  Fragments arranged by size: 

                 549     385     284
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1188    1218 
   Size:       1188      30
 
TatI w'GTAC_w

Cuts at:      0     665    1218 
   Size:        665     553
 
XmnI GAAnn'nnTTC

Cuts at:      0     233    1218 
   Size:        233     985
 

 Enzymes that do cut:

     AccI     ApoI     AseI    BanII     BbsI     BcgI     BclI    BfrBI
     BmrI     BpmI    BpuEI    BsaBI    BsaWI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI  BstZ17I     DraI   DraIII     EarI  EcoICRI  Eco57MI
    EcoRI    Hin4I  HindIII     MfeI     MslI     NdeI     NsiI     NspI
     PfoI    PshAI     SacI     SfcI     SmlI     SspI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI