Restriction map for
pF1KSDA0578
(Linear) (Six-base) MAPSORT of: KIAA0578.seq Check: 3390 from: 1 to: 4488
KIAA0578.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2473 2588 3645 4488
Size: 2473 115 1057 843
Fragments arranged by size:
2473 1057 843 115
Acc65I G'GTAC_C
Cuts at: 0 1735 4488
Size: 1735 2753
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1976 2655 3273 3300 3473 4488
Size: 928 1048 679 618 27 173 1015
Fragments arranged by size:
1048 1015 928 679 618 173 27
AfeI AGC'GCT
Cuts at: 0 3682 4488
Size: 3682 806
AflIII A'CryG_T
Cuts at: 0 1751 2298 2867 4488
Size: 1751 547 569 1621
Fragments arranged by size:
1751 1621 569 547
AhdI GACnn_n'nnGTC
Cuts at: 0 1644 1728 4488
Size: 1644 84 2760
Fragments arranged by size:
2760 1644 84
AleI CACnn'nnGTG
Cuts at: 0 1864 4488
Size: 1864 2624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2028 4488
Size: 2028 2460
AlwNI CAG_nnn'CTG
Cuts at: 0 582 2108 2692 3997 4488
Size: 582 1526 584 1305 491
Fragments arranged by size:
1526 1305 584 582 491
ApaI G_GGCC'C
Cuts at: 0 3224 4488
Size: 3224 1264
ApoI r'AATT_y
Cuts at: 0 2159 3734 4488
Size: 2159 1575 754
Fragments arranged by size:
2159 1575 754
AseI AT'TA_AT
Cuts at: 0 1031 2797 3596 4488
Size: 1031 1766 799 892
Fragments arranged by size:
1766 1031 892 799
AvaI C'yCGr_G
Cuts at: 0 2359 4193 4488
Size: 2359 1834 295
Fragments arranged by size:
2359 1834 295
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2527 2560 4488
Size: 2527 33 1928
Fragments arranged by size:
2527 1928 33
BanI G'GyrC_C
Cuts at: 0 103 334 1735 4488
Size: 103 231 1401 2753
Fragments arranged by size:
2753 1401 231 103
BanII G_rGCy'C
Cuts at: 0 536 3224 4180 4488
Size: 536 2688 956 308
Fragments arranged by size:
2688 956 536 308
BbeI G_GCGC'C
Cuts at: 0 107 338 4488
Size: 107 231 4150
Fragments arranged by size:
4150 231 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 779 1845 4488
Size: 779 1066 2643
Fragments arranged by size:
2643 1066 779
BbvCI CC'TCA_GC
Cuts at: 0 2192 4128 4488
Size: 2192 1936 360
Fragments arranged by size:
2192 1936 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3901 3935 4488
Size: 3901 34 553
Fragments arranged by size:
3901 553 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4226 4488
Size: 4226 262
BclI T'GATC_A
Cuts at: 0 2487 4488
Size: 2487 2001
BfrBI ATG'CAT
Cuts at: 0 2181 2757 4488
Size: 2181 576 1731
Fragments arranged by size:
2181 1731 576
BlpI GC'TnA_GC
Cuts at: 0 271 293 4179 4224 4488
Size: 271 22 3886 45 264
Fragments arranged by size:
3886 271 264 45 22
Bme1580I G_kGCm'C
Cuts at: 0 3224 4488
Size: 3224 1264
BmgBI CAC'GTC
Cuts at: 0 558 2299 4488
Size: 558 1741 2189
Fragments arranged by size:
2189 1741 558
BmrI ACTGGGnnnn_n'
Cuts at: 0 980 3404 4488
Size: 980 2424 1084
Fragments arranged by size:
2424 1084 980
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4194 4488
Size: 4194 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 112 256 1994 2302 2521 4488
Size: 1 111 144 1738 308 219 1967
Fragments arranged by size:
1967 1738 308 219 144 111 1
Bpu10I CC'TnA_GC
Cuts at: 0 2017 2192 2727 4128 4488
Size: 2017 175 535 1401 360
Fragments arranged by size:
2017 1401 535 360 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 484 3025 4488
Size: 157 327 2541 1463
Fragments arranged by size:
2541 1463 327 157
BsaAI yAC'GTr
Cuts at: 0 1896 2868 3383 4488
Size: 1896 972 515 1105
Fragments arranged by size:
1896 1105 972 515
BsaBI GATnn'nnATC
Cuts at: 0 1366 4488
Size: 1366 3122
BsaHI Gr'CG_yC
Cuts at: 0 104 335 3786 4488
Size: 104 231 3451 702
Fragments arranged by size:
3451 702 231 104
BsaWI w'CCGG_w
Cuts at: 0 2083 3162 4488
Size: 2083 1079 1326
Fragments arranged by size:
2083 1326 1079
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 406 421 436 3157 3187 4488
Size: 391 15 15 15 2721 30 1301
Fragments arranged by size:
2721 1301 391 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 561 676 1621 3077 3645 4300 4488
Size: 561 115 945 1456 568 655 188
Fragments arranged by size:
1456 945 655 568 561 188 115
BseYI C'CCAG_C
Cuts at: 0 68 78 1197 1234 2219 3223 4141 4429
Size: 68 10 1119 37 985 1004 918 288
Cuts at: 4429 4488
Size: 59
Fragments arranged by size:
1119 1004 985 918 288 68 59 37
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2067 4488
Size: 2067 2421
BsiEI CG_ry'CG
Cuts at: 0 261 4488
Size: 261 4227
BsiHKAI G_wGCw'C
Cuts at: 0 203 703 4180 4488
Size: 203 500 3477 308
Fragments arranged by size:
3477 500 308 203
BsmI GAATG_Cn'
Cuts at: 0 1470 2234 4488
Size: 1470 764 2254
Fragments arranged by size:
2254 1470 764
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1715 4488
Size: 1715 2773
Bsp1286I G_dGCh'C
Cuts at: 0 203 536 703 3224 4180 4488
Size: 203 333 167 2521 956 308
Fragments arranged by size:
2521 956 333 308 203 167
BspEI T'CCGG_A
Cuts at: 0 2083 3162 4488
Size: 2083 1079 1326
Fragments arranged by size:
2083 1326 1079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 567 2093 2473 2588 3241 3645 4488
Size: 567 1526 380 115 653 404 843
Fragments arranged by size:
1526 843 653 567 404 380 115
BsrBI CCG'CTC
Cuts at: 0 528 4488
Size: 528 3960
BsrDI GCAATG_nn'
Cuts at: 0 3339 3648 4488
Size: 3339 309 840
Fragments arranged by size:
3339 840 309
BsrFI r'CCGG_y
Cuts at: 0 321 742 4488
Size: 321 421 3746
Fragments arranged by size:
3746 421 321
BsrGI T'GTAC_A
Cuts at: 0 3102 4322 4488
Size: 3102 1220 166
Fragments arranged by size:
3102 1220 166
BssHII G'CGCG_C
Cuts at: 0 486 4488
Size: 486 4002
BssSI C'ACGA_G
Cuts at: 0 196 3416 3627 4488
Size: 196 3220 211 861
Fragments arranged by size:
3220 861 211 196
BstAPI GCAn_nnn'nTGC
Cuts at: 0 348 582 761 2027 2409 3233 4488
Size: 348 234 179 1266 382 824 1255
Fragments arranged by size:
1266 1255 824 382 348 234 179
BstEII G'GTnAC_C
Cuts at: 0 1256 4488
Size: 1256 3232
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 3868 4047 4263 4488
Size: 411 3457 179 216 225
Fragments arranged by size:
3457 411 225 216 179
BstYI r'GATC_y
Cuts at: 0 862 1353 1367 2688 4488
Size: 862 491 14 1321 1800
Fragments arranged by size:
1800 1321 862 491 14
BtgI C'CryG_G
Cuts at: 0 189 491 587 704 750 1371 1536 4256
Size: 189 302 96 117 46 621 165 2720
Cuts at: 4256 4488
Size: 232
Fragments arranged by size:
2720 621 302 232 189 165 117 96
46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3552 3912 4160 4488
Size: 3552 360 248 328
Fragments arranged by size:
3552 360 328 248
BtsI GCAGTG_nn'
Cuts at: 0 918 1769 3995 4440 4488
Size: 918 851 2226 445 48
Fragments arranged by size:
2226 918 851 445 48
ClaI AT'CG_AT
Cuts at: 0 1922 4488
Size: 1922 2566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1127 1162 3351 3386 4488
Size: 1127 35 2189 35 1102
Fragments arranged by size:
2189 1127 1102 35 35
DraI TTT'AAA
Cuts at: 0 936 1389 2438 3149 4488
Size: 936 453 1049 711 1339
Fragments arranged by size:
1339 1049 936 711 453
DraIII CAC_nnn'GTG
Cuts at: 0 348 2409 2946 4488
Size: 348 2061 537 1542
Fragments arranged by size:
2061 1542 537 348
DrdI GACnn_nn'nnGTC
Cuts at: 0 562 3984 4488
Size: 562 3422 504
Fragments arranged by size:
3422 562 504
EaeI y'GGCC_r
Cuts at: 0 258 311 1617 1926 3431 3667 4488
Size: 258 53 1306 309 1505 236 821
Fragments arranged by size:
1505 1306 821 309 258 236 53
EagI C'GGCC_G
Cuts at: 0 258 4488
Size: 258 4230
EarI CTCTTCn'nnn_
Cuts at: 0 388 4062 4488
Size: 388 3674 426
Fragments arranged by size:
3674 426 388
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 346 2266 4488
Size: 88 258 1920 2222
Fragments arranged by size:
2222 1920 258 88
EcoICRI GAG'CTC
Cuts at: 0 4178 4488
Size: 4178 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 112 256 928 1976 1994 2302 2521
Size: 1 111 144 672 1048 18 308 219
Cuts at: 2521 2655 3273 3300 3473 4488
Size: 134 618 27 173 1015
Fragments arranged by size:
1048 1015 672 618 308 219 173 144
134 111 27 18 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 519 4488
Size: 519 3969
EcoO109I rG'GnC_Cy
Cuts at: 0 579 1046 2105 2356 3220 4488
Size: 579 467 1059 251 864 1268
Fragments arranged by size:
1268 1059 864 579 467 251
EcoRI G'AATT_C
Cuts at: 0 3734 4488
Size: 3734 754
EcoRV GAT'ATC
Cuts at: 0 1941 2954 4488
Size: 1941 1013 1534
Fragments arranged by size:
1941 1534 1013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1608 1864 3066 4488
Size: 1608 256 1202 1422
Fragments arranged by size:
1608 1422 1202 256
FspI TGC'GCA
Cuts at: 0 352 4488
Size: 352 4136
FspAI rTGC'GCAy
Cuts at: 0 352 4488
Size: 352 4136
HaeII r_GCGC'y
Cuts at: 0 13 107 188 338 499 3684 3714 4488
Size: 13 94 81 150 161 3185 30 774
Fragments arranged by size:
3185 774 161 150 94 81 30 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 1630 1662 1714 1746 2301 2333
Size: 406 32 1192 32 52 32 555 32
Cuts at: 2333 3157 3189 4333 4365 4488
Size: 824 32 1144 32 123
Fragments arranged by size:
1192 1144 824 555 406 123 52 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 327 567 1749 2444 3989 4488
Size: 327 240 1182 695 1545 499
Fragments arranged by size:
1545 1182 695 499 327 240
HpaI GTT'AAC
Cuts at: 0 327 2444 4488
Size: 327 2117 2044
Fragments arranged by size:
2117 2044 327
KasI G'GCGC_C
Cuts at: 0 103 334 4488
Size: 103 231 4154
Fragments arranged by size:
4154 231 103
KpnI G_GTAC'C
Cuts at: 0 1739 4488
Size: 1739 2749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1203 2588 4472 4488
Size: 1203 1385 1884 16
Fragments arranged by size:
1884 1385 1203 16
MscI TGG'CCA
Cuts at: 0 1619 1928 3433 3669 4488
Size: 1619 309 1505 236 819
Fragments arranged by size:
1619 1505 819 309 236
MslI CAynn'nnrTG
Cuts at: 0 442 1389 1864 2152 2239 2752 4000 4120
Size: 442 947 475 288 87 513 1248 120
Cuts at: 4120 4261 4488
Size: 141 227
Fragments arranged by size:
1248 947 513 475 442 288 227 141
120 87
MspA1I CmG'CkG
Cuts at: 0 86 191 452 493 596 632 752 3293
Size: 86 105 261 41 103 36 120 2541
Cuts at: 3293 3665 4285 4488
Size: 372 620 203
Fragments arranged by size:
2541 620 372 261 203 120 105 103
86 41 36
NarI GG'CG_CC
Cuts at: 0 104 335 4488
Size: 104 231 4153
Fragments arranged by size:
4153 231 104
NcoI C'CATG_G
Cuts at: 0 1371 1536 4488
Size: 1371 165 2952
Fragments arranged by size:
2952 1371 165
NdeI CA'TA_TG
Cuts at: 0 2229 4488
Size: 2229 2259
NotI GC'GGCC_GC
Cuts at: 0 258 4488
Size: 258 4230
NruI TCG'CGA
Cuts at: 0 738 4488
Size: 738 3750
NsiI A_TGCA'T
Cuts at: 0 2183 2759 4488
Size: 2183 576 1729
Fragments arranged by size:
2183 1729 576
NspI r_CATG'y
Cuts at: 0 2046 2151 3109 4488
Size: 2046 105 958 1379
Fragments arranged by size:
2046 1379 958 105
PasI CC'CwG_GG
Cuts at: 0 3340 4488
Size: 3340 1148
PflMI CCAn_nnn'nTGG
Cuts at: 0 1377 4488
Size: 1377 3111
PfoI T'CCnGG_A
Cuts at: 0 233 1634 4238 4488
Size: 233 1401 2604 250
Fragments arranged by size:
2604 1401 250 233
PpuMI rG'GwC_Cy
Cuts at: 0 579 2105 2356 4488
Size: 579 1526 251 2132
Fragments arranged by size:
2132 1526 579 251
PshAI GACnn'nnGTC
Cuts at: 0 4367 4488
Size: 4367 121
PspOMI G'GGCC_C
Cuts at: 0 3220 4488
Size: 3220 1268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3503 3535 4488
Size: 3503 32 953
Fragments arranged by size:
3503 953 32
PstI C_TGCA'G
Cuts at: 0 269 766 2585 3693 4488
Size: 269 497 1819 1108 795
Fragments arranged by size:
1819 1108 795 497 269
PvuII CAG'CTG
Cuts at: 0 3293 3665 4488
Size: 3293 372 823
Fragments arranged by size:
3293 823 372
SacI G_AGCT'C
Cuts at: 0 4180 4488
Size: 4180 308
SacII CC_GC'GG
Cuts at: 0 192 494 753 4488
Size: 192 302 259 3735
Fragments arranged by size:
3735 302 259 192
SapI GCTCTTCn'nnn_
Cuts at: 0 388 4488
Size: 388 4100
SbfI CC_TGCA'GG
Cuts at: 0 3693 4488
Size: 3693 795
ScaI AGT'ACT
Cuts at: 0 1968 4488
Size: 1968 2520
SexAI A'CCwGG_T
Cuts at: 0 392 4488
Size: 392 4096
SfcI C'TryA_G
Cuts at: 0 265 762 2581 3300 3689 4488
Size: 265 497 1819 719 389 799
Fragments arranged by size:
1819 799 719 497 389 265
SfoI GGC'GCC
Cuts at: 0 105 336 4488
Size: 105 231 4152
Fragments arranged by size:
4152 231 105
SmlI C'TyrA_G
Cuts at: 0 172 499 3040 4488
Size: 172 327 2541 1448
Fragments arranged by size:
2541 1448 327 172
SpeI A'CTAG_T
Cuts at: 0 1059 4488
Size: 1059 3429
StyI C'CwwG_G
Cuts at: 0 816 1371 1536 4488
Size: 816 555 165 2952
Fragments arranged by size:
2952 816 555 165
SwaI ATTT'AAAT
Cuts at: 0 1389 3149 4488
Size: 1389 1760 1339
Fragments arranged by size:
1760 1389 1339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 399 1586 2045 3419 3487 4488
Size: 399 1187 459 1374 68 1001
Fragments arranged by size:
1374 1187 1001 459 399 68
TatI w'GTAC_w
Cuts at: 0 1966 3102 3412 3875 4322 4328 4488
Size: 1966 1136 310 463 447 6 160
Fragments arranged by size:
1966 1136 463 447 310 160 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 4488
Size: 391 32 4065
Fragments arranged by size:
4065 391 32
Tth111I GACn'n_nGTC
Cuts at: 0 3749 4488
Size: 3749 739
XbaI T'CTAG_A
Cuts at: 0 2254 2319 4488
Size: 2254 65 2169
Fragments arranged by size:
2254 2169 65
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2227 3532 3654 4026 4488
Size: 2227 1305 122 372 462
Fragments arranged by size:
2227 1305 462 372 122
XmnI GAAnn'nnTTC
Cuts at: 0 3119 4488
Size: 3119 1369
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApoI AseI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MmeI MscI MslI MspA1I NarI
NcoI NdeI NotI NruI NsiI NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PsrI PstI PvuII SacI
SacII SapI SbfI ScaI SexAI SfcI SfoI SmlI
SpeI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AloI ApaLI AscI
AsiSI AvrII BamHI BglI BglII BmtI BsaI BsiWI
BspHI BstBI BstZ17I Bsu36I FseI HindIII MfeI MluI
NaeI NgoMIV NheI PacI PciI PmeI PmlI PpiI
PsiI PspXI PvuI RsrII SalI SanDI SfiI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI StuI XhoI
XmaI ZraI