Restriction map for
pF1KA0579
(Linear) (Six-base) MAPSORT of: KIAA0579.seq Check: 4939 from: 1 to: 2847
KIAA0579.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 30 852 2847
Size: 30 822 1995
Fragments arranged by size:
1995 822 30
Acc65I G'GTAC_C
Cuts at: 0 539 664 2847
Size: 539 125 2183
Fragments arranged by size:
2183 539 125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 325 425 467 607 1007 1837 2463
Size: 6 319 100 42 140 400 830 626
Cuts at: 2463 2847
Size: 384
Fragments arranged by size:
830 626 400 384 319 140 100 42
6
AfeI AGC'GCT
Cuts at: 0 1314 2847
Size: 1314 1533
AflIII A'CryG_T
Cuts at: 0 563 1332 2847
Size: 563 769 1515
Fragments arranged by size:
1515 769 563
AlwNI CAG_nnn'CTG
Cuts at: 0 45 1491 2584 2847
Size: 45 1446 1093 263
Fragments arranged by size:
1446 1093 263 45
ApaI G_GGCC'C
Cuts at: 0 1160 2083 2847
Size: 1160 923 764
Fragments arranged by size:
1160 923 764
ApaLI G'TGCA_C
Cuts at: 0 1537 1588 2361 2548 2847
Size: 1537 51 773 187 299
Fragments arranged by size:
1537 773 299 187 51
ApoI r'AATT_y
Cuts at: 0 415 1003 1530 2847
Size: 415 588 527 1317
Fragments arranged by size:
1317 588 527 415
AscI GG'CGCG_CC
Cuts at: 0 110 2847
Size: 110 2737
AvaI C'yCGr_G
Cuts at: 0 115 1130 1232 1292 2394 2847
Size: 115 1015 102 60 1102 453
Fragments arranged by size:
1102 1015 453 115 102 60
BanI G'GyrC_C
Cuts at: 0 539 664 1082 2319 2847
Size: 539 125 418 1237 528
Fragments arranged by size:
1237 539 528 418 125
BanII G_rGCy'C
Cuts at: 0 59 735 808 1160 1306 1357 2083 2847
Size: 59 676 73 352 146 51 726 764
Fragments arranged by size:
764 726 676 352 146 73 59 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 1574 1825 2847
Size: 1574 251 1022
Fragments arranged by size:
1574 1022 251
BbvCI CC'TCA_GC
Cuts at: 0 1316 2847
Size: 1316 1531
BciVI GTATCCnnnnn_n'
Cuts at: 0 721 2847
Size: 721 2126
BfrBI ATG'CAT
Cuts at: 0 1795 2847
Size: 1795 1052
BglI GCCn_nnn'nGGC
Cuts at: 0 83 101 258 2158 2847
Size: 83 18 157 1900 689
Fragments arranged by size:
1900 689 157 83 18
BglII A'GATC_T
Cuts at: 0 993 2847
Size: 993 1854
BlpI GC'TnA_GC
Cuts at: 0 477 807 1492 2847
Size: 477 330 685 1355
Fragments arranged by size:
1355 685 477 330
Bme1580I G_kGCm'C
Cuts at: 0 1139 1160 1541 1592 1997 2083 2365 2552
Size: 1139 21 381 51 405 86 282 187
Cuts at: 2552 2847
Size: 295
Fragments arranged by size:
1139 405 381 295 282 187 86 51
21
BmgBI CAC'GTC
Cuts at: 0 1152 1333 1683 2144 2366 2847
Size: 1152 181 350 461 222 481
Fragments arranged by size:
1152 481 461 350 222 181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1467 1761 2847
Size: 1467 294 1086
Fragments arranged by size:
1467 1086 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 1242 1254 2175 2804 2847
Size: 1072 170 12 921 629 43
Fragments arranged by size:
1072 921 629 170 43 12
Bpu10I CC'TnA_GC
Cuts at: 0 913 1316 2847
Size: 913 403 1531
Fragments arranged by size:
1531 913 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 2847
Size: 1024 1823
BsaI GGTCTCn'nnnn_
Cuts at: 0 1843 2847
Size: 1843 1004
BsaAI yAC'GTr
Cuts at: 0 516 564 2847
Size: 516 48 2283
Fragments arranged by size:
2283 516 48
BsaHI Gr'CG_yC
Cuts at: 0 1873 2847
Size: 1873 974
BsaWI w'CCGG_w
Cuts at: 0 486 821 2443 2847
Size: 486 335 1622 404
Fragments arranged by size:
1622 486 404 335
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 591 621 1246 1276 2106 2136 2425 2455
Size: 591 30 625 30 830 30 289 30
Cuts at: 2455 2847
Size: 392
Fragments arranged by size:
830 625 591 392 289 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 1254 1257 1333 1564 2847
Size: 256 998 3 76 231 1283
Fragments arranged by size:
1283 998 256 231 76 3
BseYI C'CCAG_C
Cuts at: 0 144 734 1046 1876 2125 2170 2178 2185
Size: 144 590 312 830 249 45 8 7
Cuts at: 2185 2513 2847
Size: 328 334
Fragments arranged by size:
830 590 334 328 312 249 144 45
8 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1183 1456 1735 2511 2847
Size: 202 981 273 279 776 336
Fragments arranged by size:
981 776 336 279 273 202
BsiEI CG_ry'CG
Cuts at: 0 758 1862 2847
Size: 758 1104 985
Fragments arranged by size:
1104 985 758
BsiHKAI G_wGCw'C
Cuts at: 0 59 1306 1357 1541 1592 2365 2552 2847
Size: 59 1247 51 184 51 773 187 295
Fragments arranged by size:
1247 773 295 187 184 59 51 51
BsmI GAATG_Cn'
Cuts at: 0 216 2847
Size: 216 2631
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1071 2847
Size: 1071 1776
Bsp1286I G_dGCh'C
Cuts at: 0 59 735 808 1139 1160 1306 1357 1541
Size: 59 676 73 331 21 146 51 184
Cuts at: 1541 1592 1997 2083 2365 2552 2847
Size: 51 405 86 282 187 295
Fragments arranged by size:
676 405 331 295 282 187 184 146
86 73 59 51 51 21
BspEI T'CCGG_A
Cuts at: 0 486 2847
Size: 486 2361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 30 852 1707 2847
Size: 30 822 855 1140
Fragments arranged by size:
1140 855 822 30
BsrBI CCG'CTC
Cuts at: 0 728 2708 2847
Size: 728 1980 139
Fragments arranged by size:
1980 728 139
BsrDI GCAATG_nn'
Cuts at: 0 2066 2691 2847
Size: 2066 625 156
Fragments arranged by size:
2066 625 156
BsrFI r'CCGG_y
Cuts at: 0 757 1371 1833 2786 2847
Size: 757 614 462 953 61
Fragments arranged by size:
953 757 614 462 61
BsrGI T'GTAC_A
Cuts at: 0 2027 2847
Size: 2027 820
BssHII G'CGCG_C
Cuts at: 0 110 128 2847
Size: 110 18 2719
Fragments arranged by size:
2719 110 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 2360 2847
Size: 45 2315 487
Fragments arranged by size:
2315 487 45
BstEII G'GTnAC_C
Cuts at: 0 1110 2743 2847
Size: 1110 1633 104
Fragments arranged by size:
1633 1110 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1278 2847
Size: 1278 1569
BstYI r'GATC_y
Cuts at: 0 65 519 993 1624 2847
Size: 65 454 474 631 1223
Fragments arranged by size:
1223 631 474 454 65
Bsu36I CC'TnA_GG
Cuts at: 0 264 535 2847
Size: 264 271 2312
Fragments arranged by size:
2312 271 264
BtgI C'CryG_G
Cuts at: 0 96 236 741 962 1019 1277 1415 1865
Size: 96 140 505 221 57 258 138 450
Cuts at: 1865 2774 2847
Size: 909 73
Fragments arranged by size:
909 505 450 258 221 140 138 96
73 57
BtsI GCAGTG_nn'
Cuts at: 0 195 780 1125 2114 2244 2847
Size: 195 585 345 989 130 603
Fragments arranged by size:
989 603 585 345 195 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2130 2165 2847
Size: 2130 35 682
Fragments arranged by size:
2130 682 35
EaeI y'GGCC_r
Cuts at: 0 755 1859 2159 2788 2847
Size: 755 1104 300 629 59
Fragments arranged by size:
1104 755 629 300 59
EagI C'GGCC_G
Cuts at: 0 755 1859 2847
Size: 755 1104 988
Fragments arranged by size:
1104 988 755
EarI CTCTTCn'nnn_
Cuts at: 0 443 1292 2847
Size: 443 849 1555
Fragments arranged by size:
1555 849 443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 753 1395 1892 2847
Size: 753 642 497 955
Fragments arranged by size:
955 753 642 497
EcoICRI GAG'CTC
Cuts at: 0 57 1304 1355 2847
Size: 57 1247 51 1492
Fragments arranged by size:
1492 1247 57 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 325 425 467 607 1007 1072 1242
Size: 6 319 100 42 140 400 65 170
Cuts at: 1242 1254 1837 2175 2463 2804 2847
Size: 12 583 338 288 341 43
Fragments arranged by size:
583 400 341 338 319 288 170 140
100 65 43 42 12 6
EcoO109I rG'GnC_Cy
Cuts at: 0 177 260 1719 2080 2573 2847
Size: 177 83 1459 361 493 274
Fragments arranged by size:
1459 493 361 274 177 83
EcoRI G'AATT_C
Cuts at: 0 1530 2847
Size: 1530 1317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 279 2847
Size: 279 2568
HaeII r_GCGC'y
Cuts at: 0 1316 1673 2203 2513 2847
Size: 1316 357 530 310 334
Fragments arranged by size:
1316 530 357 334 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 618 650 2847
Size: 511 32 75 32 2197
Fragments arranged by size:
2197 511 75 32 32
KpnI G_GTAC'C
Cuts at: 0 543 668 2847
Size: 543 125 2179
Fragments arranged by size:
2179 543 125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1331 2847
Size: 32 1299 1516
Fragments arranged by size:
1516 1299 32
MscI TGG'CCA
Cuts at: 0 2161 2847
Size: 2161 686
MslI CAynn'nnrTG
Cuts at: 0 853 1522 1648 2847
Size: 853 669 126 1199
Fragments arranged by size:
1199 853 669 126
MspA1I CmG'CkG
Cuts at: 0 1050 1107 1227 2174 2381 2387 2498 2570
Size: 1050 57 120 947 207 6 111 72
Cuts at: 2570 2847
Size: 277
Fragments arranged by size:
1050 947 277 207 120 111 72 57
6
NaeI GCC'GGC
Cuts at: 0 759 2847
Size: 759 2088
NcoI C'CATG_G
Cuts at: 0 962 1019 1277 2774 2847
Size: 962 57 258 1497 73
Fragments arranged by size:
1497 962 258 73 57
NgoMIV G'CCGG_C
Cuts at: 0 757 2847
Size: 757 2090
NsiI A_TGCA'T
Cuts at: 0 1797 2847
Size: 1797 1050
NspI r_CATG'y
Cuts at: 0 1527 1795 2847
Size: 1527 268 1052
Fragments arranged by size:
1527 1052 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 637 2670 2847
Size: 637 2033 177
Fragments arranged by size:
2033 637 177
PmlI CAC'GTG
Cuts at: 0 516 564 2847
Size: 516 48 2283
Fragments arranged by size:
2283 516 48
PpuMI rG'GwC_Cy
Cuts at: 0 1719 2573 2847
Size: 1719 854 274
Fragments arranged by size:
1719 854 274
PshAI GACnn'nnGTC
Cuts at: 0 1783 2847
Size: 1783 1064
PspOMI G'GGCC_C
Cuts at: 0 1156 2079 2847
Size: 1156 923 768
Fragments arranged by size:
1156 923 768
PspXI vC'TCGA_Gb
Cuts at: 0 1130 2394 2847
Size: 1130 1264 453
Fragments arranged by size:
1264 1130 453
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2673 2705 2847
Size: 2673 32 142
Fragments arranged by size:
2673 142 32
PstI C_TGCA'G
Cuts at: 0 872 2090 2380 2847
Size: 872 1218 290 467
Fragments arranged by size:
1218 872 467 290
PvuII CAG'CTG
Cuts at: 0 1050 1227 2174 2387 2847
Size: 1050 177 947 213 460
Fragments arranged by size:
1050 947 460 213 177
RsrII CG'GwC_CG
Cuts at: 0 2446 2847
Size: 2446 401
SacI G_AGCT'C
Cuts at: 0 59 1306 1357 2847
Size: 59 1247 51 1490
Fragments arranged by size:
1490 1247 59 51
SanDI GG'GwC_CC
Cuts at: 0 2573 2847
Size: 2573 274
SbfI CC_TGCA'GG
Cuts at: 0 2090 2847
Size: 2090 757
ScaI AGT'ACT
Cuts at: 0 1255 2847
Size: 1255 1592
SfcI C'TryA_G
Cuts at: 0 868 2086 2229 2376 2847
Size: 868 1218 143 147 471
Fragments arranged by size:
1218 868 471 147 143
SmaI CCC'GGG
Cuts at: 0 117 1234 1294 2847
Size: 117 1117 60 1553
Fragments arranged by size:
1553 1117 117 60
SmlI C'TyrA_G
Cuts at: 0 1039 1130 2394 2847
Size: 1039 91 1264 453
Fragments arranged by size:
1264 1039 453 91
SphI G_CATG'C
Cuts at: 0 1527 1795 2847
Size: 1527 268 1052
Fragments arranged by size:
1527 1052 268
SrfI GCCC'GGGC
Cuts at: 0 117 2847
Size: 117 2730
StuI AGG'CCT
Cuts at: 0 533 690 2847
Size: 533 157 2157
Fragments arranged by size:
2157 533 157
StyI C'CwwG_G
Cuts at: 0 962 1019 1277 2664 2774 2847
Size: 962 57 258 1387 110 73
Fragments arranged by size:
1387 962 258 110 73 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1530 1770 2634 2847
Size: 1530 240 864 213
Fragments arranged by size:
1530 864 240 213
TatI w'GTAC_w
Cuts at: 0 1253 2027 2847
Size: 1253 774 820
Fragments arranged by size:
1253 820 774
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 591 623 677 709 833 865 1381 1413
Size: 591 32 54 32 124 32 516 32
Cuts at: 1413 2106 2138 2425 2457 2847
Size: 693 32 287 32 390
Fragments arranged by size:
693 591 516 390 287 124 54 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 580 2584 2847
Size: 580 2004 263
Fragments arranged by size:
2004 580 263
XbaI T'CTAG_A
Cuts at: 0 903 1605 2847
Size: 903 702 1242
Fragments arranged by size:
1242 903 702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 892 1122 2847
Size: 892 230 1725
Fragments arranged by size:
1725 892 230
XhoI C'TCGA_G
Cuts at: 0 1130 2394 2847
Size: 1130 1264 453
Fragments arranged by size:
1264 1130 453
XmaI C'CCGG_G
Cuts at: 0 115 1232 1292 2847
Size: 115 1117 60 1555
Fragments arranged by size:
1555 1117 115 60
XmnI GAAnn'nnTTC
Cuts at: 0 1390 2847
Size: 1390 1457
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlwNI ApaI ApaLI
ApoI AscI AvaI BanI BanII BbsI BbvCI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NsiI NspI PflMI PmlI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuII RsrII SacI SanDI SbfI
ScaI SfcI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI AlfI
AloI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BclI BmrI BmtI BsaBI BsiWI BspHI BssSI BstBI
BstZ17I BtgZI ClaI DraI DraIII DrdI EcoNI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI NarI NdeI NheI NotI NruI PacI PasI
PciI PfoI PmeI PpiI PsiI PvuI SacII SalI
SapI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI ZraI