Restriction map for
pF1KA0583
(Linear) (Six-base) MAPSORT of: KIAA0583.seq Check: 5641 from: 1 to: 5757
KIAA0583.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 251 852 1221 3269 3639 5757
Size: 251 601 369 2048 370 2118
Fragments arranged by size:
2118 2048 601 370 369 251
AccI GT'mk_AC
Cuts at: 0 5366 5757
Size: 5366 391
Acc65I G'GTAC_C
Cuts at: 0 4342 5757
Size: 4342 1415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 739 1066 1546 1756 2398 2834 3257
Size: 592 147 327 480 210 642 436 423
Cuts at: 3257 4723 4747 5024 5757
Size: 1466 24 277 733
Fragments arranged by size:
1466 733 642 592 480 436 423 327
277 210 147 24
AfeI AGC'GCT
Cuts at: 0 104 5757
Size: 104 5653
AflII C'TTAA_G
Cuts at: 0 2004 5757
Size: 2004 3753
AflIII A'CryG_T
Cuts at: 0 4500 5757
Size: 4500 1257
AhdI GACnn_n'nnGTC
Cuts at: 0 2313 3933 5757
Size: 2313 1620 1824
Fragments arranged by size:
2313 1824 1620
AleI CACnn'nnGTG
Cuts at: 0 1231 1972 2866 3112 3559 4547 5757
Size: 1231 741 894 246 447 988 1210
Fragments arranged by size:
1231 1210 988 894 741 447 246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4219 5757
Size: 4219 1538
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1768 1800 5757
Size: 1768 32 3957
Fragments arranged by size:
3957 1768 32
AlwNI CAG_nnn'CTG
Cuts at: 0 45 210 612 762 1524 2812 2872 2983
Size: 45 165 402 150 762 1288 60 111
Cuts at: 2983 3396 3716 4182 5047 5757
Size: 413 320 466 865 710
Fragments arranged by size:
1288 865 762 710 466 413 402 320
165 150 111 60 45
ApaI G_GGCC'C
Cuts at: 0 2773 4800 5757
Size: 2773 2027 957
Fragments arranged by size:
2773 2027 957
ApaLI G'TGCA_C
Cuts at: 0 4291 4540 5757
Size: 4291 249 1217
Fragments arranged by size:
4291 1217 249
ApoI r'AATT_y
Cuts at: 0 4978 5757
Size: 4978 779
AvaI C'yCGr_G
Cuts at: 0 79 88 470 1944 3141 3169 3638 4003
Size: 79 9 382 1474 1197 28 469 365
Cuts at: 4003 4162 5757
Size: 159 1595
Fragments arranged by size:
1595 1474 1197 469 382 365 159 79
28 9
BanI G'GyrC_C
Cuts at: 0 148 387 3456 4342 4369 5757
Size: 148 239 3069 886 27 1388
Fragments arranged by size:
3069 1388 886 239 148 27
BanII G_rGCy'C
Cuts at: 0 8 488 1745 2398 2466 2680 2773 3179
Size: 8 480 1257 653 68 214 93 406
Cuts at: 3179 3446 3568 3865 4069 4800 5676 5757
Size: 267 122 297 204 731 876 81
Fragments arranged by size:
1257 876 731 653 480 406 297 267
214 204 122 93 81 68 8
BbeI G_GCGC'C
Cuts at: 0 152 391 5757
Size: 152 239 5366
Fragments arranged by size:
5366 239 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 235 1243 2564 4492 4500 5757
Size: 235 1008 1321 1928 8 1257
Fragments arranged by size:
1928 1321 1257 1008 235 8
BbvCI CC'TCA_GC
Cuts at: 0 361 739 1676 3960 5757
Size: 361 378 937 2284 1797
Fragments arranged by size:
2284 1797 937 378 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1232 1266 1484 1518 3132 3166 3310 3344
Size: 1232 34 218 34 1614 34 144 34
Cuts at: 3344 3446 3480 3780 3814 4071 4105 5757
Size: 102 34 300 34 257 34 1652
Fragments arranged by size:
1652 1614 1232 300 257 218 144 102
34 34 34 34 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2077 5757
Size: 2077 3680
BglII A'GATC_T
Cuts at: 0 5696 5757
Size: 5696 61
BlpI GC'TnA_GC
Cuts at: 0 2854 4300 4993 5757
Size: 2854 1446 693 764
Fragments arranged by size:
2854 1446 764 693
Bme1580I G_kGCm'C
Cuts at: 0 376 852 2773 3459 3529 4295 4544 4800
Size: 376 476 1921 686 70 766 249 256
Cuts at: 4800 4888 5757
Size: 88 869
Fragments arranged by size:
1921 869 766 686 476 376 256 249
88 70
BmgBI CAC'GTC
Cuts at: 0 729 2727 3162 4662 5250 5757
Size: 729 1998 435 1500 588 507
Fragments arranged by size:
1998 1500 729 588 507 435
BmrI ACTGGGnnnn_n'
Cuts at: 0 24 1581 1758 1912 2648 3344 3662 4269
Size: 24 1557 177 154 736 696 318 607
Cuts at: 4269 5757
Size: 1488
Fragments arranged by size:
1557 1488 736 696 607 318 177 154
24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 462 2898 3108 4163 5757
Size: 462 2436 210 1055 1594
Fragments arranged by size:
2436 1594 1055 462 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1033 1522 2221 3103 3356 3418 3430
Size: 244 789 489 699 882 253 62 12
Cuts at: 3430 3753 4204 4342 4443 4513 4549 4663 4968
Size: 323 451 138 101 70 36 114 305
Cuts at: 4968 5757
Size: 789
Fragments arranged by size:
882 789 789 699 489 451 323 305
253 244 138 114 101 70 62 36
12
Bpu10I CC'TnA_GC
Cuts at: 0 361 739 1003 1676 2674 3691 3703 3960
Size: 361 378 264 673 998 1017 12 257
Cuts at: 3960 5757
Size: 1797
Fragments arranged by size:
1797 1017 998 673 378 361 264 257
12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1363 1729 5326 5647 5757
Size: 154 1209 366 3597 321 110
Fragments arranged by size:
3597 1209 366 321 154 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 2752 5549 5757
Size: 2752 2797 208
Fragments arranged by size:
2797 2752 208
BsaAI yAC'GTr
Cuts at: 0 4050 4290 4863 5757
Size: 4050 240 573 894
Fragments arranged by size:
4050 894 573 240
BsaHI Gr'CG_yC
Cuts at: 0 149 269 388 953 3687 4718 5410 5757
Size: 149 120 119 565 2734 1031 692 347
Fragments arranged by size:
2734 1031 692 565 347 149 120 119
BsaWI w'CCGG_w
Cuts at: 0 1619 5757
Size: 1619 4138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 389 419 1768 1798 2503 2533 3280 3310
Size: 389 30 1349 30 705 30 747 30
Cuts at: 3310 4816 4846 5432 5462 5757
Size: 1506 30 586 30 295
Fragments arranged by size:
1506 1349 747 705 586 389 295 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 397 664 832 1042 1134 1282 1429
Size: 151 246 267 168 210 92 148 147
Cuts at: 1429 2074 2388 3243 3893 4051 4762 5684 5757
Size: 645 314 855 650 158 711 922 73
Fragments arranged by size:
922 855 711 650 645 314 267 246
210 168 158 151 148 147 92 73
BseYI C'CCAG_C
Cuts at: 0 8 851 1020 1067 1462 3002 3315 3550
Size: 8 843 169 47 395 1540 313 235
Cuts at: 3550 4077 4440 4523 4954 5324 5757
Size: 527 363 83 431 370 433
Fragments arranged by size:
1540 843 527 433 431 395 370 363
313 235 169 83 47 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2143 4078 4753 5033 5190 5757
Size: 2143 1935 675 280 157 567
Fragments arranged by size:
2143 1935 675 567 280 157
BsiEI CG_ry'CG
Cuts at: 0 3361 4809 5757
Size: 3361 1448 948
Fragments arranged by size:
3361 1448 948
BsiHKAI G_wGCw'C
Cuts at: 0 80 488 1745 2398 2680 3446 3568 4295
Size: 80 408 1257 653 282 766 122 727
Cuts at: 4295 4544 5757
Size: 249 1213
Fragments arranged by size:
1257 1213 766 727 653 408 282 249
122 80
BsmI GAATG_Cn'
Cuts at: 0 3474 3898 4929 5592 5757
Size: 3474 424 1031 663 165
Fragments arranged by size:
3474 1031 663 424 165
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1870 4125 5757
Size: 1870 2255 1632
Fragments arranged by size:
2255 1870 1632
Bsp1286I G_dGCh'C
Cuts at: 0 8 80 376 488 852 1745 2398 2466
Size: 8 72 296 112 364 893 653 68
Cuts at: 2466 2680 2773 3179 3446 3459 3529 3568 3865
Size: 214 93 406 267 13 70 39 297
Cuts at: 3865 4069 4295 4544 4800 4888 5676 5757
Size: 204 226 249 256 88 788 81
Fragments arranged by size:
893 788 653 406 364 297 296 267
256 249 226 214 204 112 93 88
81 72 70 68 39 13 8
BspEI T'CCGG_A
Cuts at: 0 1619 5757
Size: 1619 4138
BspHI T'CATG_A
Cuts at: 0 1304 5757
Size: 1304 4453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 251 611 785 852 1184 1221 2622
Size: 218 33 360 174 67 332 37 1401
Cuts at: 2622 2978 3269 3639 3724 4211 4568 5123 5633
Size: 356 291 370 85 487 357 555 510
Cuts at: 5633 5757
Size: 124
Fragments arranged by size:
1401 555 510 487 370 360 357 356
332 291 218 174 124 85 67 37
33
BsrBI CCG'CTC
Cuts at: 0 120 192 1272 1650 2687 4011 5212 5340
Size: 120 72 1080 378 1037 1324 1201 128
Cuts at: 5340 5478 5754 5757
Size: 138 276 3
Fragments arranged by size:
1324 1201 1080 1037 378 276 138 128
120 72 3
BsrDI GCAATG_nn'
Cuts at: 0 1988 2297 3215 3970 5456 5711 5757
Size: 1988 309 918 755 1486 255 46
Fragments arranged by size:
1988 1486 918 755 309 255 46
BsrFI r'CCGG_y
Cuts at: 0 416 692 1562 2735 3264 3332 3482 4339
Size: 416 276 870 1173 529 68 150 857
Cuts at: 4339 4808 5054 5757
Size: 469 246 703
Fragments arranged by size:
1173 870 857 703 529 469 416 276
246 150 68
BsrGI T'GTAC_A
Cuts at: 0 1283 5757
Size: 1283 4474
BssSI C'ACGA_G
Cuts at: 0 814 2400 4621 5757
Size: 814 1586 2221 1136
Fragments arranged by size:
2221 1586 1136 814
BstAPI GCAn_nnn'nTGC
Cuts at: 0 612 762 5291 5757
Size: 612 150 4529 466
Fragments arranged by size:
4529 612 466 150
BstBI TT'CG_AA
Cuts at: 0 1494 5757
Size: 1494 4263
BstEII G'GTnAC_C
Cuts at: 0 838 4128 5757
Size: 838 3290 1629
Fragments arranged by size:
3290 1629 838
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2109 3114 4366 5757
Size: 2109 1005 1252 1391
Fragments arranged by size:
2109 1391 1252 1005
BstYI r'GATC_y
Cuts at: 0 575 4934 5696 5757
Size: 575 4359 762 61
Fragments arranged by size:
4359 762 575 61
Bsu36I CC'TnA_GG
Cuts at: 0 1117 1639 4745 5569 5757
Size: 1117 522 3106 824 188
Fragments arranged by size:
3106 1117 824 522 188
BtgI C'CryG_G
Cuts at: 0 95 917 1157 1838 2466 2706 2861 3747
Size: 95 822 240 681 628 240 155 886
Cuts at: 3747 3847 4133 4214 5757
Size: 100 286 81 1543
Fragments arranged by size:
1543 886 822 681 628 286 240 240
155 100 95 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4128 5757
Size: 4128 1629
BtsI GCAGTG_nn'
Cuts at: 0 372 1186 1507 2743 2873 3760 4255 4371
Size: 372 814 321 1236 130 887 495 116
Cuts at: 4371 5442 5757
Size: 1071 315
Fragments arranged by size:
1236 1071 887 814 495 372 321 315
130 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2154 2189 5757
Size: 2154 35 3568
Fragments arranged by size:
3568 2154 35
DraIII CAC_nnn'GTG
Cuts at: 0 1630 5757
Size: 1630 4127
EaeI y'GGCC_r
Cuts at: 0 182 656 914 1082 1364 1406 1553 1820
Size: 182 474 258 168 282 42 147 267
Cuts at: 1820 1998 2409 2733 2740 3266 3334 3358 3419
Size: 178 411 324 7 526 68 24 61
Cuts at: 3419 4577 5385 5757
Size: 1158 808 372
Fragments arranged by size:
1158 808 526 474 411 372 324 282
267 258 182 178 168 147 68 61
42 24 7
EagI C'GGCC_G
Cuts at: 0 3358 5757
Size: 3358 2399
EarI CTCTTCn'nnn_
Cuts at: 0 634 958 5757
Size: 634 324 4799
Fragments arranged by size:
4799 634 324
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 172 1561 2136 3826 4711 5757
Size: 172 1389 575 1690 885 1046
Fragments arranged by size:
1690 1389 1046 885 575 172
EcoICRI GAG'CTC
Cuts at: 0 486 1743 2396 2678 3444 3566 5757
Size: 486 1257 653 282 766 122 2191
Fragments arranged by size:
2191 1257 766 653 486 282 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 592 739 1033 1066 1522 1546 1756
Size: 244 348 147 294 33 456 24 210
Cuts at: 1756 2221 2398 2834 3103 3257 3356 3418 3430
Size: 465 177 436 269 154 99 62 12
Cuts at: 3430 3753 4204 4342 4443 4513 4549 4663 4723
Size: 323 451 138 101 70 36 114 60
Cuts at: 4723 4747 4968 5024 5757
Size: 24 221 56 733
Fragments arranged by size:
733 465 456 451 436 348 323 294
269 244 221 210 177 154 147 138
114 101 99 70 62 60 56 36
33 24 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 543 639 1540 2377 2800 3012 5757
Size: 49 494 96 901 837 423 212 2745
Fragments arranged by size:
2745 901 837 494 423 212 96 49
EcoO109I rG'GnC_Cy
Cuts at: 0 207 536 1173 1680 2258 2575 2623 2770
Size: 207 329 637 507 578 317 48 147
Cuts at: 2770 2834 2980 3022 3288 3713 4426 4609 4632
Size: 64 146 42 266 425 713 183 23
Cuts at: 4632 4767 4796 4797 4895 5236 5757
Size: 135 29 1 98 341 521
Fragments arranged by size:
713 637 578 521 507 425 341 329
317 266 207 183 147 146 135 98
64 48 42 29 23 1
EcoRI G'AATT_C
Cuts at: 0 4978 5757
Size: 4978 779
EcoRV GAT'ATC
Cuts at: 0 3844 5757
Size: 3844 1913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 195 2278 2934 5757
Size: 195 2083 656 2823
Fragments arranged by size:
2823 2083 656 195
FspI TGC'GCA
Cuts at: 0 1687 3598 5757
Size: 1687 1911 2159
Fragments arranged by size:
2159 1911 1687
HaeII r_GCGC'y
Cuts at: 0 15 106 152 223 391 498 1429 2083
Size: 15 91 46 71 168 107 931 654
Cuts at: 2083 3149 3776 4214 5027 5534 5757
Size: 1066 627 438 813 507 223
Fragments arranged by size:
1066 931 813 654 627 507 438 223
168 107 91 71 46 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 2264 2296 2299 2331 3235 3267
Size: 222 32 2010 32 3 32 904 32
Cuts at: 3267 3280 3312 3372 3404 3919 3951 4375 4407
Size: 13 32 60 32 515 32 424 32
Cuts at: 4407 5432 5464 5546 5578 5757
Size: 1025 32 82 32 179
Fragments arranged by size:
2010 1025 904 515 424 222 179 82
60 32 32 32 32 32 32 32
32 32 32 13 3
HincII GTy'rAC
Cuts at: 0 3990 5367 5757
Size: 3990 1377 390
Fragments arranged by size:
3990 1377 390
HindIII A'AGCT_T
Cuts at: 0 3364 5623 5757
Size: 3364 2259 134
Fragments arranged by size:
3364 2259 134
KasI G'GCGC_C
Cuts at: 0 148 387 5757
Size: 148 239 5370
Fragments arranged by size:
5370 239 148
KpnI G_GTAC'C
Cuts at: 0 4346 5757
Size: 4346 1411
MluI A'CGCG_T
Cuts at: 0 4500 5757
Size: 4500 1257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 908 1232 1748 1812 2846 3779 5757
Size: 578 330 324 516 64 1034 933 1978
Fragments arranged by size:
1978 1034 933 578 516 330 324 64
MscI TGG'CCA
Cuts at: 0 184 658 1555 2411 5757
Size: 184 474 897 856 3346
Fragments arranged by size:
3346 897 856 474 184
MslI CAynn'nnrTG
Cuts at: 0 916 1231 1972 2107 2113 2866 3112 3559
Size: 916 315 741 135 6 753 246 447
Cuts at: 3559 3668 4547 5393 5757
Size: 109 879 846 364
Fragments arranged by size:
916 879 846 753 741 447 364 315
246 135 109 6
MspA1I CmG'CkG
Cuts at: 0 97 129 456 759 1020 1038 1092 1794
Size: 97 32 327 303 261 18 54 702
Cuts at: 1794 2157 2224 2828 3849 4077 4527 4914 5044
Size: 363 67 604 1021 228 450 387 130
Cuts at: 5044 5093 5187 5757
Size: 49 94 570
Fragments arranged by size:
1021 702 604 570 450 387 363 327
303 261 228 130 97 94 67 54
49 32 18
NaeI GCC'GGC
Cuts at: 0 694 1564 2737 3266 3334 3484 5757
Size: 694 870 1173 529 68 150 2273
Fragments arranged by size:
2273 1173 870 694 529 150 68
NarI GG'CG_CC
Cuts at: 0 149 388 5757
Size: 149 239 5369
Fragments arranged by size:
5369 239 149
NcoI C'CATG_G
Cuts at: 0 917 1838 2466 3747 5757
Size: 917 921 628 1281 2010
Fragments arranged by size:
2010 1281 921 917 628
NgoMIV G'CCGG_C
Cuts at: 0 692 1562 2735 3264 3332 3482 5757
Size: 692 870 1173 529 68 150 2275
Fragments arranged by size:
2275 1173 870 692 529 150 68
NspI r_CATG'y
Cuts at: 0 738 5001 5493 5757
Size: 738 4263 492 264
Fragments arranged by size:
4263 738 492 264
PasI CC'CwG_GG
Cuts at: 0 2133 2617 3654 4709 4881 5757
Size: 2133 484 1037 1055 172 876
Fragments arranged by size:
2133 1055 1037 876 484 172
PflMI CCAn_nnn'nTGG
Cuts at: 0 1844 2617 2983 3306 5199 5757
Size: 1844 773 366 323 1893 558
Fragments arranged by size:
1893 1844 773 558 366 323
PfoI T'CCnGG_A
Cuts at: 0 203 1538 1580 3395 3446 4459 4640 4897
Size: 203 1335 42 1815 51 1013 181 257
Cuts at: 4897 4984 5450 5757
Size: 87 466 307
Fragments arranged by size:
1815 1335 1013 466 307 257 203 181
87 51 42
PmlI CAC'GTG
Cuts at: 0 4050 4290 5757
Size: 4050 240 1467
Fragments arranged by size:
4050 1467 240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1768 1800 5757
Size: 1768 32 3957
Fragments arranged by size:
3957 1768 32
PpuMI rG'GwC_Cy
Cuts at: 0 207 1173 2258 2623 2834 2980 3288 3713
Size: 207 966 1085 365 211 146 308 425
Cuts at: 3713 4609 4632 4895 5757
Size: 896 23 263 862
Fragments arranged by size:
1085 966 896 862 425 365 308 263
211 207 146 23
PsiI TTA'TAA
Cuts at: 0 2891 5757
Size: 2891 2866
PspOMI G'GGCC_C
Cuts at: 0 2769 4796 5757
Size: 2769 2027 961
Fragments arranged by size:
2769 2027 961
PspXI vC'TCGA_Gb
Cuts at: 0 88 4162 5757
Size: 88 4074 1595
Fragments arranged by size:
4074 1595 88
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3571 3603 5757
Size: 3571 32 2154
Fragments arranged by size:
3571 2154 32
PstI C_TGCA'G
Cuts at: 0 608 617 785 1097 1181 1202 1349 3622
Size: 608 9 168 312 84 21 147 2273
Cuts at: 3622 3721 5296 5757
Size: 99 1575 461
Fragments arranged by size:
2273 1575 608 461 312 168 147 99
84 21 9
PvuII CAG'CTG
Cuts at: 0 129 456 759 1020 1092 1794 4527 4914
Size: 129 327 303 261 72 702 2733 387
Cuts at: 4914 5757
Size: 843
Fragments arranged by size:
2733 843 702 387 327 303 261 129
72
SacI G_AGCT'C
Cuts at: 0 488 1745 2398 2680 3446 3568 5757
Size: 488 1257 653 282 766 122 2189
Fragments arranged by size:
2189 1257 766 653 488 282 122
SacII CC_GC'GG
Cuts at: 0 98 3850 5757
Size: 98 3752 1907
Fragments arranged by size:
3752 1907 98
SalI G'TCGA_C
Cuts at: 0 5365 5757
Size: 5365 392
SanDI GG'GwC_CC
Cuts at: 0 2258 2623 5757
Size: 2258 365 3134
Fragments arranged by size:
3134 2258 365
SexAI A'CCwGG_T
Cuts at: 0 1319 5757
Size: 1319 4438
SfcI C'TryA_G
Cuts at: 0 312 604 613 781 1093 1177 1198 1345
Size: 312 292 9 168 312 84 21 147
Cuts at: 1345 3618 3717 5292 5757
Size: 2273 99 1575 465
Fragments arranged by size:
2273 1575 465 312 312 292 168 147
99 84 21 9
SfoI GGC'GCC
Cuts at: 0 150 389 5757
Size: 150 239 5368
Fragments arranged by size:
5368 239 150
SmaI CCC'GGG
Cuts at: 0 472 1946 3171 5757
Size: 472 1474 1225 2586
Fragments arranged by size:
2586 1474 1225 472
SmlI C'TyrA_G
Cuts at: 0 88 169 1378 1744 2004 4162 5305 5626
Size: 88 81 1209 366 260 2158 1143 321
Cuts at: 5626 5757
Size: 131
Fragments arranged by size:
2158 1209 1143 366 321 260 131 88
81
SpeI A'CTAG_T
Cuts at: 0 3133 5757
Size: 3133 2624
StuI AGG'CCT
Cuts at: 0 2647 2791 4026 5220 5320 5757
Size: 2647 144 1235 1194 100 437
Fragments arranged by size:
2647 1235 1194 437 144 100
StyI C'CwwG_G
Cuts at: 0 152 185 659 917 1838 2466 2843 3747
Size: 152 33 474 258 921 628 377 904
Cuts at: 3747 4124 4604 4763 5099 5123 5757
Size: 377 480 159 336 24 634
Fragments arranged by size:
921 904 634 628 480 474 377 377
336 258 159 152 33 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1228 1366 2125 2776 3760 5757
Size: 1228 138 759 651 984 1997
Fragments arranged by size:
1997 1228 984 759 651 138
TatI w'GTAC_w
Cuts at: 0 1283 1292 4277 5636 5757
Size: 1283 9 2985 1359 121
Fragments arranged by size:
2985 1359 1283 121 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 1165 1197 1614 1646 2503 2535
Size: 389 32 744 32 417 32 857 32
Cuts at: 2535 3394 3426 4300 4332 5757
Size: 859 32 874 32 1425
Fragments arranged by size:
1425 874 859 857 744 417 389 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1321 2367 5037 5757
Size: 1321 1046 2670 720
Fragments arranged by size:
2670 1321 1046 720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 924 1416 3777 4131 4266 4326 4531
Size: 63 861 492 2361 354 135 60 205
Cuts at: 4531 5757
Size: 1226
Fragments arranged by size:
2361 1226 861 492 354 205 135 63
60
XhoI C'TCGA_G
Cuts at: 0 88 4162 5757
Size: 88 4074 1595
Fragments arranged by size:
4074 1595 88
XmaI C'CCGG_G
Cuts at: 0 470 1944 3169 5757
Size: 470 1474 1225 2588
Fragments arranged by size:
2588 1474 1225 470
XmnI GAAnn'nnTTC
Cuts at: 0 1490 2383 5757
Size: 1490 893 3374
Fragments arranged by size:
3374 1490 893
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuII
SacI SacII SalI SanDI SexAI SfcI SfoI SmaI
SmlI SpeI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AgeI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BmtI BsaBI BsiWI BssHII
BstZ17I ClaI DraI DrdI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PshAI PvuI RsrII SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SphI SrfI SspI SwaI XbaI ZraI