Restriction map for
pF1KSDA0588
(Linear) (Six-base) MAPSORT of: KIAA0588.seq Check: 4304 from: 1 to: 2796
KIAA0588.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2740 2796
Size: 2740 56
AccI GT'mk_AC
Cuts at: 0 2684 2796
Size: 2684 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2165 2796
Size: 2165 631
AflIII A'CryG_T
Cuts at: 0 1511 1634 2796
Size: 1511 123 1162
Fragments arranged by size:
1511 1162 123
AleI CACnn'nnGTG
Cuts at: 0 1720 2796
Size: 1720 1076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 796 2796
Size: 796 2000
AlwNI CAG_nnn'CTG
Cuts at: 0 442 2796
Size: 442 2354
AseI AT'TA_AT
Cuts at: 0 1093 2796
Size: 1093 1703
AvaI C'yCGr_G
Cuts at: 0 167 733 1678 2032 2796
Size: 167 566 945 354 764
Fragments arranged by size:
945 764 566 354 167
BamHI G'GATC_C
Cuts at: 0 474 2796
Size: 474 2322
BanI G'GyrC_C
Cuts at: 0 670 1613 2473 2506 2605 2611 2796
Size: 670 943 860 33 99 6 185
Fragments arranged by size:
943 860 670 185 99 33 6
BanII G_rGCy'C
Cuts at: 0 167 176 284 515 1555 1751 1893 2369
Size: 167 9 108 231 1040 196 142 476
Cuts at: 2369 2590 2796
Size: 221 206
Fragments arranged by size:
1040 476 231 221 206 196 167 142
108 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1344 2100 2796
Size: 1344 756 696
Fragments arranged by size:
1344 756 696
BbvCI CC'TCA_GC
Cuts at: 0 1624 2796
Size: 1624 1172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1484 1518 2796
Size: 1484 34 1278
Fragments arranged by size:
1484 1278 34
BclI T'GATC_A
Cuts at: 0 1001 2796
Size: 1001 1795
BlpI GC'TnA_GC
Cuts at: 0 514 728 1549 2796
Size: 514 214 821 1247
Fragments arranged by size:
1247 821 514 214
Bme1580I G_kGCm'C
Cuts at: 0 775 1616 2666 2796
Size: 775 841 1050 130
Fragments arranged by size:
1050 841 775 130
BmgBI CAC'GTC
Cuts at: 0 1017 2796
Size: 1017 1779
BmrI ACTGGGnnnn_n'
Cuts at: 0 1541 2637 2796
Size: 1541 1096 159
Fragments arranged by size:
1541 1096 159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 1426 1927 2796
Size: 141 1285 501 869
Fragments arranged by size:
1285 869 501 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 136 181 2024 2796
Size: 129 7 45 1843 772
Fragments arranged by size:
1843 772 129 45 7
Bpu10I CC'TnA_GC
Cuts at: 0 1624 2140 2796
Size: 1624 516 656
Fragments arranged by size:
1624 656 516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1810 1897 2796
Size: 1810 87 899
Fragments arranged by size:
1810 899 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 67 2103 2460 2796
Size: 67 2036 357 336
Fragments arranged by size:
2036 357 336 67
BsaAI yAC'GTr
Cuts at: 0 1512 2661 2796
Size: 1512 1149 135
Fragments arranged by size:
1512 1149 135
BsaHI Gr'CG_yC
Cuts at: 0 2209 2560 2796
Size: 2209 351 236
Fragments arranged by size:
2209 351 236
BsaWI w'CCGG_w
Cuts at: 0 76 105 477 753 842 2796
Size: 76 29 372 276 89 1954
Fragments arranged by size:
1954 372 276 89 76 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 344 374 1774 1804 2796
Size: 344 30 1400 30 992
Fragments arranged by size:
1400 992 344 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 943 1415 2796
Size: 292 651 472 1381
Fragments arranged by size:
1381 651 472 292
BseYI C'CCAG_C
Cuts at: 0 45 2312 2745 2796
Size: 45 2267 433 51
Fragments arranged by size:
2267 433 51 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 1846 2257 2796
Size: 1 1845 411 539
Fragments arranged by size:
1845 539 411 1
BsiHKAI G_wGCw'C
Cuts at: 0 176 284 515 1555 2590 2796
Size: 176 108 231 1040 1035 206
Fragments arranged by size:
1040 1035 231 206 176 108
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1419 1898 2796
Size: 1419 479 898
Fragments arranged by size:
1419 898 479
Bsp1286I G_dGCh'C
Cuts at: 0 167 176 284 515 775 1555 1616 1751
Size: 167 9 108 231 260 780 61 135
Cuts at: 1751 1893 2369 2590 2666 2796
Size: 142 476 221 76 130
Fragments arranged by size:
780 476 260 231 221 167 142 135
130 108 76 61 9
BspEI T'CCGG_A
Cuts at: 0 105 477 753 2796
Size: 105 372 276 2043
Fragments arranged by size:
2043 372 276 105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2740 2796
Size: 2740 56
BsrBI CCG'CTC
Cuts at: 0 1739 2796
Size: 1739 1057
BsrDI GCAATG_nn'
Cuts at: 0 968 2706 2763 2796
Size: 968 1738 57 33
Fragments arranged by size:
1738 968 57 33
BsrFI r'CCGG_y
Cuts at: 0 1347 2205 2503 2608 2796
Size: 1347 858 298 105 188
Fragments arranged by size:
1347 858 298 188 105
BssHII G'CGCG_C
Cuts at: 0 676 2796
Size: 676 2120
BssSI C'ACGA_G
Cuts at: 0 925 2263 2796
Size: 925 1338 533
Fragments arranged by size:
1338 925 533
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2661 2796
Size: 2661 135
BstEII G'GTnAC_C
Cuts at: 0 1761 2796
Size: 1761 1035
BstXI CCAn_nnnn'nTGG
Cuts at: 0 153 1722 1773 2556 2796
Size: 153 1569 51 783 240
Fragments arranged by size:
1569 783 240 153 51
BstYI r'GATC_y
Cuts at: 0 474 1682 2796
Size: 474 1208 1114
Fragments arranged by size:
1208 1114 474
Bsu36I CC'TnA_GG
Cuts at: 0 1358 2796
Size: 1358 1438
BtgI C'CryG_G
Cuts at: 0 1298 1703 1944 1982 1988 2093 2279 2615
Size: 1298 405 241 38 6 105 186 336
Cuts at: 2615 2796
Size: 181
Fragments arranged by size:
1298 405 336 241 186 181 105 38
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2510 2796
Size: 2510 286
DraI TTT'AAA
Cuts at: 0 1106 1176 2796
Size: 1106 70 1620
Fragments arranged by size:
1620 1106 70
DraIII CAC_nnn'GTG
Cuts at: 0 2220 2796
Size: 2220 576
DrdI GACnn_nn'nnGTC
Cuts at: 0 814 2335 2796
Size: 814 1521 461
Fragments arranged by size:
1521 814 461
EaeI y'GGCC_r
Cuts at: 0 1794 1931 1947 1985 1991 2090 2796
Size: 1794 137 16 38 6 99 706
Fragments arranged by size:
1794 706 137 99 38 16 6
EarI CTCTTCn'nnn_
Cuts at: 0 104 1439 1852 2287 2796
Size: 104 1335 413 435 509
Fragments arranged by size:
1335 509 435 413 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1953 2032 2553 2796
Size: 1953 79 521 243
Fragments arranged by size:
1953 521 243 79
EcoICRI GAG'CTC
Cuts at: 0 174 282 513 1553 2588 2796
Size: 174 108 231 1040 1035 208
Fragments arranged by size:
1040 1035 231 208 174 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 136 181 2024 2165 2796
Size: 129 7 45 1843 141 631
Fragments arranged by size:
1843 631 141 129 45 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 809 2173 2796
Size: 809 1364 623
Fragments arranged by size:
1364 809 623
EcoO109I rG'GnC_Cy
Cuts at: 0 150 2742 2796
Size: 150 2592 54
Fragments arranged by size:
2592 150 54
EcoRV GAT'ATC
Cuts at: 0 483 2796
Size: 483 2313
FspI TGC'GCA
Cuts at: 0 664 1879 2796
Size: 664 1215 917
Fragments arranged by size:
1215 917 664
FspAI rTGC'GCAy
Cuts at: 0 664 1879 2796
Size: 664 1215 917
Fragments arranged by size:
1215 917 664
HaeII r_GCGC'y
Cuts at: 0 2140 2154 2206 2630 2796
Size: 2140 14 52 424 166
Fragments arranged by size:
2140 424 166 52 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 980 1012 1130 1162 1774 1806
Size: 179 32 769 32 118 32 612 32
Cuts at: 1806 2572 2604 2796
Size: 766 32 192
Fragments arranged by size:
769 766 612 192 179 118 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 792 2796
Size: 792 2004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 899 1550 2796
Size: 668 231 651 1246
Fragments arranged by size:
1246 668 651 231
MslI CAynn'nnrTG
Cuts at: 0 325 445 685 1261 1720 2536 2796
Size: 325 120 240 576 459 816 260
Fragments arranged by size:
816 576 459 325 260 240 120
MspA1I CmG'CkG
Cuts at: 0 242 780 2095 2480 2722 2796
Size: 242 538 1315 385 242 74
Fragments arranged by size:
1315 538 385 242 242 74
NaeI GCC'GGC
Cuts at: 0 2207 2610 2796
Size: 2207 403 186
Fragments arranged by size:
2207 403 186
NcoI C'CATG_G
Cuts at: 0 2615 2796
Size: 2615 181
NgoMIV G'CCGG_C
Cuts at: 0 2205 2608 2796
Size: 2205 403 188
Fragments arranged by size:
2205 403 188
NspI r_CATG'y
Cuts at: 0 2334 2796
Size: 2334 462
PasI CC'CwG_GG
Cuts at: 0 2595 2796
Size: 2595 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 2013 2752 2796
Size: 2013 739 44
Fragments arranged by size:
2013 739 44
PfoI T'CCnGG_A
Cuts at: 0 1937 2796
Size: 1937 859
PmlI CAC'GTG
Cuts at: 0 2661 2796
Size: 2661 135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 2796
Size: 436 32 2328
Fragments arranged by size:
2328 436 32
PpuMI rG'GwC_Cy
Cuts at: 0 150 2796
Size: 150 2646
PspXI vC'TCGA_Gb
Cuts at: 0 2032 2796
Size: 2032 764
PstI C_TGCA'G
Cuts at: 0 503 2177 2252 2657 2796
Size: 503 1674 75 405 139
Fragments arranged by size:
1674 503 405 139 75
PvuII CAG'CTG
Cuts at: 0 780 2722 2796
Size: 780 1942 74
Fragments arranged by size:
1942 780 74
SacI G_AGCT'C
Cuts at: 0 176 284 515 1555 2590 2796
Size: 176 108 231 1040 1035 206
Fragments arranged by size:
1040 1035 231 206 176 108
SacII CC_GC'GG
Cuts at: 0 2096 2796
Size: 2096 700
SapI GCTCTTCn'nnn_
Cuts at: 0 104 2796
Size: 104 2692
SexAI A'CCwGG_T
Cuts at: 0 626 1757 2075 2796
Size: 626 1131 318 721
Fragments arranged by size:
1131 721 626 318
SfcI C'TryA_G
Cuts at: 0 499 1203 2173 2248 2653 2796
Size: 499 704 970 75 405 143
Fragments arranged by size:
970 704 499 405 143 75
SgrAI Cr'CCGG_yG
Cuts at: 0 2205 2796
Size: 2205 591
SmaI CCC'GGG
Cuts at: 0 169 2796
Size: 169 2627
SmlI C'TyrA_G
Cuts at: 0 1825 1912 2032 2796
Size: 1825 87 120 764
Fragments arranged by size:
1825 764 120 87
StuI AGG'CCT
Cuts at: 0 1363 2796
Size: 1363 1433
StyI C'CwwG_G
Cuts at: 0 767 1158 1488 1766 2615 2796
Size: 767 391 330 278 849 181
Fragments arranged by size:
849 767 391 330 278 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 2796
Size: 100 2696
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2254 2286 2796
Size: 2254 32 510
Fragments arranged by size:
2254 510 32
Tth111I GACn'n_nGTC
Cuts at: 0 1222 2796
Size: 1222 1574
XbaI T'CTAG_A
Cuts at: 0 315 2796
Size: 315 2481
XhoI C'TCGA_G
Cuts at: 0 2032 2796
Size: 2032 764
XmaI C'CCGG_G
Cuts at: 0 167 2796
Size: 167 2629
XmnI GAAnn'nnTTC
Cuts at: 0 1241 2796
Size: 1241 1555
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI AseI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FspI FspAI HaeII Hin4I
HincII MmeI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpiI PpuMI PspXI PstI
PvuII SacI SacII SapI SexAI SfcI SgrAI SmaI
SmlI StuI StyI TaqII TstI Tth111I XbaI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BbeI
BciVI BfrBI BglI BglII BmtI BsaBI BsiEI BsiWI
BsmI BspHI BsrGI BstBI BstZ17I BtsI ClaI CspCI
EagI EcoRI FalI FseI HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PshAI PsiI PspOMI PsrI
PvuI RsrII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SnaBI SpeI SphI SrfI SspI SwaI TatI
XcmI ZraI