Restriction map for
pF1KSDA0592
(Linear) (Six-base) MAPSORT of: KIAA0592.seq Check: 2084 from: 1 to: 3960
KIAA0592.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 3051 3960
Size: 3051 909
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1300 2888 3960
Size: 980 320 1588 1072
Fragments arranged by size:
1588 1072 980 320
AhdI GACnn_n'nnGTC
Cuts at: 0 1917 3960
Size: 1917 2043
AleI CACnn'nnGTG
Cuts at: 0 1249 3960
Size: 1249 2711
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3080 3960
Size: 3080 880
AlwNI CAG_nnn'CTG
Cuts at: 0 30 1222 3960
Size: 30 1192 2738
Fragments arranged by size:
2738 1192 30
ApoI r'AATT_y
Cuts at: 0 145 2260 2814 3960
Size: 145 2115 554 1146
Fragments arranged by size:
2115 1146 554 145
AvaI C'yCGr_G
Cuts at: 0 2953 3960
Size: 2953 1007
BanI G'GyrC_C
Cuts at: 0 33 1392 2163 2590 3151 3960
Size: 33 1359 771 427 561 809
Fragments arranged by size:
1359 809 771 561 427 33
BanII G_rGCy'C
Cuts at: 0 50 1823 3445 3960
Size: 50 1773 1622 515
Fragments arranged by size:
1773 1622 515 50
BbeI G_GCGC'C
Cuts at: 0 3155 3960
Size: 3155 805
BbsI GAAGACnn'nnnn_
Cuts at: 0 237 364 967 1175 2006 2053 2081 2508
Size: 237 127 603 208 831 47 28 427
Cuts at: 2508 3209 3960
Size: 701 751
Fragments arranged by size:
831 751 701 603 427 237 208 127
47 28
BbvCI CC'TCA_GC
Cuts at: 0 332 3104 3960
Size: 332 2772 856
Fragments arranged by size:
2772 856 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2473 2507 3960
Size: 2473 34 1453
Fragments arranged by size:
2473 1453 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3853 3960
Size: 3853 107
BglII A'GATC_T
Cuts at: 0 381 1464 2311 2861 3960
Size: 381 1083 847 550 1099
Fragments arranged by size:
1099 1083 847 550 381
Bme1580I G_kGCm'C
Cuts at: 0 3355 3960
Size: 3355 605
BmgBI CAC'GTC
Cuts at: 0 1703 3960
Size: 1703 2257
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 3960
Size: 270 3690
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1644 2080 2172 3960
Size: 1068 576 436 92 1788
Fragments arranged by size:
1788 1068 576 436 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1181 1936 3284 3960
Size: 1088 93 755 1348 676
Fragments arranged by size:
1348 1088 755 676 93
Bpu10I CC'TnA_GC
Cuts at: 0 332 1298 2318 2993 3086 3104 3960
Size: 332 966 1020 675 93 18 856
Fragments arranged by size:
1020 966 856 675 332 93 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1558 3960
Size: 310 1248 2402
Fragments arranged by size:
2402 1248 310
BsaBI GATnn'nnATC
Cuts at: 0 2654 3482 3960
Size: 2654 828 478
Fragments arranged by size:
2654 828 478
BsaHI Gr'CG_yC
Cuts at: 0 40 3152 3960
Size: 40 3112 808
Fragments arranged by size:
3112 808 40
BsaWI w'CCGG_w
Cuts at: 0 8 3960
Size: 8 3952
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 49 67 79 460 490 1154 1184
Size: 37 12 18 12 381 30 664 30
Cuts at: 1184 1858 1888 2913 2943 3817 3847 3960
Size: 674 30 1025 30 874 30 113
Fragments arranged by size:
1025 874 674 664 381 113 37 30
30 30 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 328 694 697 817 934 1132 1192
Size: 91 237 366 3 120 117 198 60
Cuts at: 1192 1636 1879 2056 2102 3229 3825 3960
Size: 444 243 177 46 1127 596 135
Fragments arranged by size:
1127 596 444 366 243 237 198 177
135 120 117 91 60 46 3
BseYI C'CCAG_C
Cuts at: 0 1212 2678 2829 3960
Size: 1212 1466 151 1131
Fragments arranged by size:
1466 1212 1131 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3086 3221 3863 3960
Size: 3086 135 642 97
Fragments arranged by size:
3086 642 135 97
BsiEI CG_ry'CG
Cuts at: 0 64 116 3960
Size: 64 52 3844
Fragments arranged by size:
3844 64 52
BsiHKAI G_wGCw'C
Cuts at: 0 1823 2509 3960
Size: 1823 686 1451
Fragments arranged by size:
1823 1451 686
BsmI GAATG_Cn'
Cuts at: 0 2334 3948 3960
Size: 2334 1614 12
Fragments arranged by size:
2334 1614 12
Bsp1286I G_dGCh'C
Cuts at: 0 50 1823 2509 3355 3445 3960
Size: 50 1773 686 846 90 515
Fragments arranged by size:
1773 846 686 515 90 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1907 3960
Size: 1907 2053
BsrBI CCG'CTC
Cuts at: 0 60 3960
Size: 60 3900
BsrDI GCAATG_nn'
Cuts at: 0 2024 3131 3960
Size: 2024 1107 829
Fragments arranged by size:
2024 1107 829
BsrFI r'CCGG_y
Cuts at: 0 36 2968 3960
Size: 36 2932 992
Fragments arranged by size:
2932 992 36
BssSI C'ACGA_G
Cuts at: 0 362 3960
Size: 362 3598
BstEII G'GTnAC_C
Cuts at: 0 1548 3960
Size: 1548 2412
BstYI r'GATC_y
Cuts at: 0 80 381 528 1464 2311 2861 3358 3960
Size: 80 301 147 936 847 550 497 602
Fragments arranged by size:
936 847 602 550 497 301 147 80
Bsu36I CC'TnA_GG
Cuts at: 0 939 2807 3960
Size: 939 1868 1153
Fragments arranged by size:
1868 1153 939
BtgI C'CryG_G
Cuts at: 0 64 902 2362 2925 3347 3368 3960
Size: 64 838 1460 563 422 21 592
Fragments arranged by size:
1460 838 592 563 422 64 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1475 2198 3960
Size: 1475 723 1762
Fragments arranged by size:
1762 1475 723
BtsI GCAGTG_nn'
Cuts at: 0 642 1433 1863 3480 3960
Size: 642 791 430 1617 480
Fragments arranged by size:
1617 791 642 480 430
DraIII CAC_nnn'GTG
Cuts at: 0 3915 3960
Size: 3915 45
EaeI y'GGCC_r
Cuts at: 0 61 113 3168 3175 3960
Size: 61 52 3055 7 785
Fragments arranged by size:
3055 785 61 52 7
EagI C'GGCC_G
Cuts at: 0 61 113 3960
Size: 61 52 3847
Fragments arranged by size:
3847 61 52
EarI CTCTTCn'nnn_
Cuts at: 0 662 671 752 1001 1135 1727 1793 2186
Size: 662 9 81 249 134 592 66 393
Cuts at: 2186 2414 2522 3960
Size: 228 108 1438
Fragments arranged by size:
1438 662 592 393 249 228 134 108
81 66 9
EcoICRI GAG'CTC
Cuts at: 0 1821 3960
Size: 1821 2139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1088 1181 1300 1936 2888 3284 3960
Size: 980 108 93 119 636 952 396 676
Fragments arranged by size:
980 952 676 636 396 119 108 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3245 3458 3960
Size: 3245 213 502
Fragments arranged by size:
3245 502 213
EcoO109I rG'GnC_Cy
Cuts at: 0 170 1946 3127 3219 3960
Size: 170 1776 1181 92 741
Fragments arranged by size:
1776 1181 741 170 92
EcoRI G'AATT_C
Cuts at: 0 145 3960
Size: 145 3815
EcoRV GAT'ATC
Cuts at: 0 456 1443 3741 3960
Size: 456 987 2298 219
Fragments arranged by size:
2298 987 456 219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1569 2032 2551 3960
Size: 1569 463 519 1409
Fragments arranged by size:
1569 1409 519 463
HaeII r_GCGC'y
Cuts at: 0 3155 3960
Size: 3155 805
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 1171 1203 1372 1404 1764 1796
Size: 670 32 469 32 169 32 360 32
Cuts at: 1796 1903 1935 2913 2945 2967 2999 3580 3612
Size: 107 32 978 32 22 32 581 32
Cuts at: 3612 3817 3849 3960
Size: 205 32 111
Fragments arranged by size:
978 670 581 469 360 205 169 111
107 32 32 32 32 32 32 32
32 32 22
HincII GTy'rAC
Cuts at: 0 2577 2730 3960
Size: 2577 153 1230
Fragments arranged by size:
2577 1230 153
HpaI GTT'AAC
Cuts at: 0 2730 3960
Size: 2730 1230
KasI G'GCGC_C
Cuts at: 0 3151 3960
Size: 3151 809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 3944 3960
Size: 23 3921 16
Fragments arranged by size:
3921 23 16
MscI TGG'CCA
Cuts at: 0 3170 3960
Size: 3170 790
MslI CAynn'nnrTG
Cuts at: 0 710 742 1249 2361 3960
Size: 710 32 507 1112 1599
Fragments arranged by size:
1599 1112 710 507 32
MspA1I CmG'CkG
Cuts at: 0 1759 2833 2851 3229 3235 3960
Size: 1759 1074 18 378 6 725
Fragments arranged by size:
1759 1074 725 378 18 6
NaeI GCC'GGC
Cuts at: 0 38 3960
Size: 38 3922
NarI GG'CG_CC
Cuts at: 0 3152 3960
Size: 3152 808
NcoI C'CATG_G
Cuts at: 0 902 2362 3960
Size: 902 1460 1598
Fragments arranged by size:
1598 1460 902
NdeI CA'TA_TG
Cuts at: 0 2788 3960
Size: 2788 1172
NgoMIV G'CCGG_C
Cuts at: 0 36 3960
Size: 36 3924
PasI CC'CwG_GG
Cuts at: 0 1934 3960
Size: 1934 2026
PflMI CCAn_nnn'nTGG
Cuts at: 0 30 2623 2685 2931 3427 3960
Size: 30 2593 62 246 496 533
Fragments arranged by size:
2593 533 496 246 62 30
PfoI T'CCnGG_A
Cuts at: 0 206 398 2519 3539 3960
Size: 206 192 2121 1020 421
Fragments arranged by size:
2121 1020 421 206 192
PpuMI rG'GwC_Cy
Cuts at: 0 170 1946 3127 3960
Size: 170 1776 1181 833
Fragments arranged by size:
1776 1181 833 170
PvuII CAG'CTG
Cuts at: 0 1759 3229 3960
Size: 1759 1470 731
Fragments arranged by size:
1759 1470 731
SacI G_AGCT'C
Cuts at: 0 1823 3960
Size: 1823 2137
SanDI GG'GwC_CC
Cuts at: 0 1946 3960
Size: 1946 2014
SapI GCTCTTCn'nnn_
Cuts at: 0 2522 3960
Size: 2522 1438
ScaI AGT'ACT
Cuts at: 0 323 3960
Size: 323 3637
SfcI C'TryA_G
Cuts at: 0 130 139 629 3960
Size: 130 9 490 3331
Fragments arranged by size:
3331 490 130 9
SfoI GGC'GCC
Cuts at: 0 3153 3960
Size: 3153 807
SmaI CCC'GGG
Cuts at: 0 2955 3960
Size: 2955 1005
SmlI C'TyrA_G
Cuts at: 0 325 1573 3960
Size: 325 1248 2387
Fragments arranged by size:
2387 1248 325
SspI AAT'ATT
Cuts at: 0 1881 3960
Size: 1881 2079
StyI C'CwwG_G
Cuts at: 0 902 1312 1967 2030 2246 2362 3241 3386
Size: 902 410 655 63 216 116 879 145
Cuts at: 3386 3960
Size: 574
Fragments arranged by size:
902 879 655 574 410 216 145 116
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1054 3415 3960
Size: 1054 2361 545
Fragments arranged by size:
2361 1054 545
TatI w'GTAC_w
Cuts at: 0 321 3960
Size: 321 3639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 49 69 81 1409 1441 1858 1890
Size: 37 12 20 12 1328 32 417 32
Cuts at: 1890 2109 2141 3901 3933 3960
Size: 219 32 1760 32 27
Fragments arranged by size:
1760 1328 417 219 37 32 32 32
32 27 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 0 1776 3960
Size: 1776 2184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 909 3420 3786 3960
Size: 909 2511 366 174
Fragments arranged by size:
2511 909 366 174
XmaI C'CCGG_G
Cuts at: 0 2953 3960
Size: 2953 1007
XmnI GAAnn'nnTTC
Cuts at: 0 993 2762 3960
Size: 993 1769 1198
Fragments arranged by size:
1769 1198 993
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HpaI KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV PasI PflMI
PfoI PpuMI PvuII SacI SanDI SapI ScaI SfcI
SfoI SmaI SmlI SspI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BclI BfrBI BglI BlpI BmtI BsaI BsaAI
BsiWI BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI
BstXI BstZ17I ClaI CspCI DraI DrdI EciI FseI
FspI FspAI HindIII KpnI MfeI MluI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI StuI SwaI XbaI XhoI ZraI