Restriction map for
pF1KA0594
(Linear) (Six-base) MAPSORT of: KIAA0594.seq Check: 1599 from: 1 to: 3303
KIAA0594.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AatII G_ACGT'C
Cuts at: 0 25 3303
Size: 25 3278
AccI GT'mk_AC
Cuts at: 0 3212 3303
Size: 3212 91
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1171 2639 3004 3303
Size: 1048 123 1468 365 299
Fragments arranged by size:
1468 1048 365 299 123
AflII C'TTAA_G
Cuts at: 0 651 1524 3303
Size: 651 873 1779
Fragments arranged by size:
1779 873 651
AflIII A'CryG_T
Cuts at: 0 919 1385 3303
Size: 919 466 1918
Fragments arranged by size:
1918 919 466
AhdI GACnn_n'nnGTC
Cuts at: 0 72 3303
Size: 72 3231
ApoI r'AATT_y
Cuts at: 0 99 556 1426 1982 2824 2909 2926 3249
Size: 99 457 870 556 842 85 17 323
Cuts at: 3249 3303
Size: 54
Fragments arranged by size:
870 842 556 457 323 99 85 54
17
AseI AT'TA_AT
Cuts at: 0 2816 3303
Size: 2816 487
AvaI C'yCGr_G
Cuts at: 0 60 84 673 3303
Size: 60 24 589 2630
Fragments arranged by size:
2630 589 60 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2510 2543 3303
Size: 2510 33 760
Fragments arranged by size:
2510 760 33
BanI G'GyrC_C
Cuts at: 0 3275 3303
Size: 3275 28
BanII G_rGCy'C
Cuts at: 0 57 1451 3303
Size: 57 1394 1852
Fragments arranged by size:
1852 1394 57
BbeI G_GCGC'C
Cuts at: 0 3279 3303
Size: 3279 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 25 1962 3303
Size: 25 1937 1341
Fragments arranged by size:
1937 1341 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 2163 3303
Size: 2163 1140
BglII A'GATC_T
Cuts at: 0 1654 3303
Size: 1654 1649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1304 3303
Size: 1304 1999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2068 3303
Size: 2068 1235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2113 2492 3303
Size: 2113 379 811
Fragments arranged by size:
2113 811 379
BsaI GGTCTCn'nnnn_
Cuts at: 0 59 670 3303
Size: 59 611 2633
Fragments arranged by size:
2633 611 59
BsaAI yAC'GTr
Cuts at: 0 1388 2029 3303
Size: 1388 641 1274
Fragments arranged by size:
1388 1274 641
BsaHI Gr'CG_yC
Cuts at: 0 22 3276 3303
Size: 22 3254 27
Fragments arranged by size:
3254 27 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 12 42 1104 1134 3149 3179 3303
Size: 12 30 1062 30 2015 30 124
Fragments arranged by size:
2015 1062 124 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 1125 2544 2951 3303
Size: 574 551 1419 407 352
Fragments arranged by size:
1419 574 551 407 352
BseYI C'CCAG_C
Cuts at: 0 38 3303
Size: 38 3265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2510 3303
Size: 2510 793
BsiHKAI G_wGCw'C
Cuts at: 0 57 2054 3303
Size: 57 1997 1249
Fragments arranged by size:
1997 1249 57
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1215 3303
Size: 1215 2088
Bsp1286I G_dGCh'C
Cuts at: 0 57 1451 2054 3303
Size: 57 1394 603 1249
Fragments arranged by size:
1394 1249 603 57
BspHI T'CATG_A
Cuts at: 0 1758 3303
Size: 1758 1545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 309 3303
Size: 309 2994
BstBI TT'CG_AA
Cuts at: 0 2913 2931 3303
Size: 2913 18 372
Fragments arranged by size:
2913 372 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3245 3303
Size: 3245 58
BstYI r'GATC_y
Cuts at: 0 1654 3303
Size: 1654 1649
Bsu36I CC'TnA_GG
Cuts at: 0 539 3303
Size: 539 2764
BtgI C'CryG_G
Cuts at: 0 811 1166 2564 3303
Size: 811 355 1398 739
Fragments arranged by size:
1398 811 739 355
BtsI GCAGTG_nn'
Cuts at: 0 2009 2857 3303
Size: 2009 848 446
Fragments arranged by size:
2009 848 446
DraI TTT'AAA
Cuts at: 0 1431 2153 2795 3254 3303
Size: 1431 722 642 459 49
Fragments arranged by size:
1431 722 642 459 49
EaeI y'GGCC_r
Cuts at: 0 2366 3303
Size: 2366 937
EarI CTCTTCn'nnn_
Cuts at: 0 337 479 875 1088 1269 2180 2186 2507
Size: 337 142 396 213 181 911 6 321
Cuts at: 2507 3303
Size: 796
Fragments arranged by size:
911 796 396 337 321 213 181 142
6
EcoICRI GAG'CTC
Cuts at: 0 55 3303
Size: 55 3248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1171 2068 2639 3004 3303
Size: 1048 123 897 571 365 299
Fragments arranged by size:
1048 897 571 365 299 123
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1573 3303
Size: 1573 1730
EcoO109I rG'GnC_Cy
Cuts at: 0 81 3303
Size: 81 3222
EcoRI G'AATT_C
Cuts at: 0 99 2909 3303
Size: 99 2810 394
Fragments arranged by size:
2810 394 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2260 3303
Size: 2260 1043
HaeII r_GCGC'y
Cuts at: 0 3279 3303
Size: 3279 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 58 90 2490 2522 3303
Size: 12 32 14 32 2400 32 781
Fragments arranged by size:
2400 781 32 32 32 14 12
HincII GTy'rAC
Cuts at: 0 26 2708 3303
Size: 26 2682 595
Fragments arranged by size:
2682 595 26
HpaI GTT'AAC
Cuts at: 0 2708 3303
Size: 2708 595
KasI G'GCGC_C
Cuts at: 0 3275 3303
Size: 3275 28
MfeI C'AATT_G
Cuts at: 0 605 2663 3303
Size: 605 2058 640
Fragments arranged by size:
2058 640 605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 216 531 3303
Size: 56 160 315 2772
Fragments arranged by size:
2772 315 160 56
MslI CAynn'nnrTG
Cuts at: 0 169 1757 2972 3303
Size: 169 1588 1215 331
Fragments arranged by size:
1588 1215 331 169
MspA1I CmG'CkG
Cuts at: 0 114 120 3303
Size: 114 6 3183
Fragments arranged by size:
3183 114 6
NarI GG'CG_CC
Cuts at: 0 3276 3303
Size: 3276 27
NcoI C'CATG_G
Cuts at: 0 811 2564 3303
Size: 811 1753 739
Fragments arranged by size:
1753 811 739
NdeI CA'TA_TG
Cuts at: 0 189 1632 3227 3303
Size: 189 1443 1595 76
Fragments arranged by size:
1595 1443 189 76
NspI r_CATG'y
Cuts at: 0 923 3303
Size: 923 2380
PciI A'CATG_T
Cuts at: 0 919 3303
Size: 919 2384
PfoI T'CCnGG_A
Cuts at: 0 210 3303
Size: 210 3093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 3303
Size: 336 32 2935
Fragments arranged by size:
2935 336 32
PpuMI rG'GwC_Cy
Cuts at: 0 81 3303
Size: 81 3222
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 845 877 1793 1825 3303
Size: 845 32 916 32 1478
Fragments arranged by size:
1478 916 845 32 32
PvuII CAG'CTG
Cuts at: 0 114 3303
Size: 114 3189
SacI G_AGCT'C
Cuts at: 0 57 3303
Size: 57 3246
SapI GCTCTTCn'nnn_
Cuts at: 0 1088 2186 3303
Size: 1088 1098 1117
Fragments arranged by size:
1117 1098 1088
ScaI AGT'ACT
Cuts at: 0 2940 3303
Size: 2940 363
SfcI C'TryA_G
Cuts at: 0 706 1769 2324 3303
Size: 706 1063 555 979
Fragments arranged by size:
1063 979 706 555
SfoI GGC'GCC
Cuts at: 0 3277 3303
Size: 3277 26
SmlI C'TyrA_G
Cuts at: 0 651 673 1524 2092 2507 3303
Size: 651 22 851 568 415 796
Fragments arranged by size:
851 796 651 568 415 22
SpeI A'CTAG_T
Cuts at: 0 858 3303
Size: 858 2445
SspI AAT'ATT
Cuts at: 0 507 3303
Size: 507 2796
StyI C'CwwG_G
Cuts at: 0 282 811 2564 3303
Size: 282 529 1753 739
Fragments arranged by size:
1753 739 529 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 804 884 1118 2476 3303
Size: 804 80 234 1358 827
Fragments arranged by size:
1358 827 804 234 80
TatI w'GTAC_w
Cuts at: 0 2278 2938 3303
Size: 2278 660 365
Fragments arranged by size:
2278 660 365
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3149 3181 3303
Size: 3149 32 122
Fragments arranged by size:
3149 122 32
XbaI T'CTAG_A
Cuts at: 0 355 1290 3303
Size: 355 935 2013
Fragments arranged by size:
2013 935 355
XhoI C'TCGA_G
Cuts at: 0 673 3303
Size: 673 2630
XmnI GAAnn'nnTTC
Cuts at: 0 830 2991 3303
Size: 830 2161 312
Fragments arranged by size:
2161 830 312
ZraI GAC'GTC
Cuts at: 0 23 3303
Size: 23 3280
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AhdI ApoI AseI
AvaI BaeI BanI BanII BbeI BbsI BciVI BglII
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BstBI
BstXI BstYI Bsu36I BtgI BtsI DraI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HpaI KasI MfeI MmeI MslI MspA1I NarI
NcoI NdeI NspI PciI PfoI PpiI PpuMI PsrI
PvuII SacI SapI ScaI SfcI SfoI SmlI SpeI
SspI StyI TaqII TatI TstI XbaI XhoI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AleI AlfI AloI
AlwNI ApaI ApaLI AscI AsiSI AvrII BamHI BbvCI
BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BsaBI BsaWI BsiEI BsiWI BsmI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoRV FseI FspI FspAI HindIII KpnI MluI MscI
NaeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PstI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI
Tth111I XcmI XmaI