Restriction map for
pF1KA0599
(Linear) (Six-base) MAPSORT of: KIAA0599.seq Check: 9380 from: 1 to: 4020
KIAA0599.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 713 1367 4020
Size: 713 654 2653
Fragments arranged by size:
2653 713 654
AatII G_ACGT'C
Cuts at: 0 3512 4020
Size: 3512 508
AccI GT'mk_AC
Cuts at: 0 1013 3140 3264 4020
Size: 1013 2127 124 756
Fragments arranged by size:
2127 1013 756 124
Acc65I G'GTAC_C
Cuts at: 0 1256 4020
Size: 1256 2764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 694 1300 1496 1943 2547 2780 3480
Size: 409 285 606 196 447 604 233 700
Cuts at: 3480 4020
Size: 540
Fragments arranged by size:
700 606 604 540 447 409 285 233
196
AflII C'TTAA_G
Cuts at: 0 2077 4020
Size: 2077 1943
AgeI A'CCGG_T
Cuts at: 0 2456 3870 4020
Size: 2456 1414 150
Fragments arranged by size:
2456 1414 150
AhdI GACnn_n'nnGTC
Cuts at: 0 2235 4020
Size: 2235 1785
AleI CACnn'nnGTG
Cuts at: 0 1318 3210 4020
Size: 1318 1892 810
Fragments arranged by size:
1892 1318 810
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2299 2345 4020
Size: 2299 46 1675
Fragments arranged by size:
2299 1675 46
AlwNI CAG_nnn'CTG
Cuts at: 0 200 256 333 636 1474 2779 4020
Size: 200 56 77 303 838 1305 1241
Fragments arranged by size:
1305 1241 838 303 200 77 56
ApaI G_GGCC'C
Cuts at: 0 221 884 1940 2718 4020
Size: 221 663 1056 778 1302
Fragments arranged by size:
1302 1056 778 663 221
AvaI C'yCGr_G
Cuts at: 0 379 1385 1454 1754 2495 3005 3957 4020
Size: 379 1006 69 300 741 510 952 63
Fragments arranged by size:
1006 952 741 510 379 300 69 63
BanI G'GyrC_C
Cuts at: 0 1256 1331 2116 3606 4020
Size: 1256 75 785 1490 414
Fragments arranged by size:
1490 1256 785 414 75
BanII G_rGCy'C
Cuts at: 0 82 116 221 664 884 1622 1940 2257
Size: 82 34 105 443 220 738 318 317
Cuts at: 2257 2426 2698 2718 2729 2845 3250 3475 3521
Size: 169 272 20 11 116 405 225 46
Cuts at: 3521 4020
Size: 499
Fragments arranged by size:
738 499 443 405 318 317 272 225
220 169 116 105 82 46 34 20
11
BbeI G_GCGC'C
Cuts at: 0 1335 4020
Size: 1335 2685
BbsI GAAGACnn'nnnn_
Cuts at: 0 2453 2822 4020
Size: 2453 369 1198
Fragments arranged by size:
2453 1198 369
BbvCI CC'TCA_GC
Cuts at: 0 2344 3478 4020
Size: 2344 1134 542
Fragments arranged by size:
2344 1134 542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1836 1870 4020
Size: 1836 34 2150
Fragments arranged by size:
2150 1836 34
BfrBI ATG'CAT
Cuts at: 0 590 4020
Size: 590 3430
BglI GCCn_nnn'nGGC
Cuts at: 0 2297 3001 3686 3968 4020
Size: 2297 704 685 282 52
Fragments arranged by size:
2297 704 685 282 52
BglII A'GATC_T
Cuts at: 0 2810 4020
Size: 2810 1210
BlpI GC'TnA_GC
Cuts at: 0 271 2287 3034 4020
Size: 271 2016 747 986
Fragments arranged by size:
2016 986 747 271
Bme1580I G_kGCm'C
Cuts at: 0 221 884 922 1940 2718 3260 4020
Size: 221 663 38 1018 778 542 760
Fragments arranged by size:
1018 778 760 663 542 221 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1808 1934 2437 4020
Size: 1808 126 503 1583
Fragments arranged by size:
1808 1583 503 126
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2604 3540 4020
Size: 2604 936 480
Fragments arranged by size:
2604 936 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 697 829 934 1591 1779 1958 2005
Size: 177 520 132 105 657 188 179 47
Cuts at: 2005 2314 2610 2654 2929 3299 3489 3796 3859
Size: 309 296 44 275 370 190 307 63
Cuts at: 3859 4020
Size: 161
Fragments arranged by size:
657 520 370 309 307 296 275 190
188 179 177 161 132 105 63 47
44
Bpu10I CC'TnA_GC
Cuts at: 0 1363 2086 2344 3478 3664 4020
Size: 1363 723 258 1134 186 356
Fragments arranged by size:
1363 1134 723 356 258 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 4020
Size: 679 3341
BsaI GGTCTCn'nnnn_
Cuts at: 0 455 1569 1829 2492 3484 3739 4020
Size: 455 1114 260 663 992 255 281
Fragments arranged by size:
1114 992 663 455 281 260 255
BsaAI yAC'GTr
Cuts at: 0 324 3099 3114 4020
Size: 324 2775 15 906
Fragments arranged by size:
2775 906 324 15
BsaBI GATnn'nnATC
Cuts at: 0 1048 4020
Size: 1048 2972
BsaHI Gr'CG_yC
Cuts at: 0 1332 1954 3509 4020
Size: 1332 622 1555 511
Fragments arranged by size:
1555 1332 622 511
BsaWI w'CCGG_w
Cuts at: 0 2456 2833 3167 3870 4020
Size: 2456 377 334 703 150
Fragments arranged by size:
2456 703 377 334 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 550 580 841 871 1956 1986
Size: 175 30 345 30 261 30 1085 30
Cuts at: 1986 2608 2638 4020
Size: 622 30 1382
Fragments arranged by size:
1382 1085 622 345 261 175 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 60 72 124 445 834 853 1026 1153
Size: 60 12 52 321 389 19 173 127
Cuts at: 1153 1507 1522 1525 1537 1540 1543 1546 1549
Size: 354 15 3 12 3 3 3 3
Cuts at: 1549 1552 1555 1648 1651 1654 1657 1660 1669
Size: 3 3 93 3 3 3 3 9
Cuts at: 1669 1963 2110 2926 2953 2958 3366 3856 4020
Size: 294 147 816 27 5 408 490 164
Fragments arranged by size:
816 490 408 389 354 321 294 173
164 147 127 93 60 52 27 19
15 12 12 9 5 3 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 115 1609 1900 2922 3382 3820 3882 4020
Size: 115 1494 291 1022 460 438 62 138
Fragments arranged by size:
1494 1022 460 438 291 138 115 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 3055 3106 4020
Size: 242 2813 51 914
Fragments arranged by size:
2813 914 242 51
BsiEI CG_ry'CG
Cuts at: 0 46 2704 3570 4020
Size: 46 2658 866 450
Fragments arranged by size:
2658 866 450 46
BsiHKAI G_wGCw'C
Cuts at: 0 833 1036 2068 2288 2729 3359 3521 3670
Size: 833 203 1032 220 441 630 162 149
Cuts at: 3670 4020
Size: 350
Fragments arranged by size:
1032 833 630 441 350 220 203 162
149
BsmI GAATG_Cn'
Cuts at: 0 592 4020
Size: 592 3428
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1945 4020
Size: 1945 2075
Bsp1286I G_dGCh'C
Cuts at: 0 82 116 221 664 833 884 922 1036
Size: 82 34 105 443 169 51 38 114
Cuts at: 1036 1622 1940 2068 2257 2288 2426 2698 2718
Size: 586 318 128 189 31 138 272 20
Cuts at: 2718 2729 2845 3250 3260 3359 3475 3521 3670
Size: 11 116 405 10 99 116 46 149
Cuts at: 3670 4020
Size: 350
Fragments arranged by size:
586 443 405 350 318 272 189 169
149 138 128 116 116 114 105 99
82 51 46 38 34 31 20 11
10
BspEI T'CCGG_A
Cuts at: 0 2833 3167 4020
Size: 2833 334 853
Fragments arranged by size:
2833 853 334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 677 713 1367 1668 2894 3879 4020
Size: 341 336 36 654 301 1226 985 141
Fragments arranged by size:
1226 985 654 341 336 301 141 36
BsrBI CCG'CTC
Cuts at: 0 42 1275 4020
Size: 42 1233 2745
Fragments arranged by size:
2745 1233 42
BsrDI GCAATG_nn'
Cuts at: 0 951 2174 4020
Size: 951 1223 1846
Fragments arranged by size:
1846 1223 951
BsrFI r'CCGG_y
Cuts at: 0 45 164 626 1334 1820 2456 2501 3312
Size: 45 119 462 708 486 636 45 811
Cuts at: 3312 3455 3569 3785 3870 4020
Size: 143 114 216 85 150
Fragments arranged by size:
811 708 636 486 462 216 150 143
119 114 85 45 45
BstAPI GCAn_nnn'nTGC
Cuts at: 0 256 636 651 939 1414 4020
Size: 256 380 15 288 475 2606
Fragments arranged by size:
2606 475 380 288 256 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 28 570 1134 2607 2875 3212 3709 4020
Size: 28 542 564 1473 268 337 497 311
Fragments arranged by size:
1473 564 542 497 337 311 268 28
BstYI r'GATC_y
Cuts at: 0 2810 4020
Size: 2810 1210
Bsu36I CC'TnA_GG
Cuts at: 0 1895 4020
Size: 1895 2125
BtgI C'CryG_G
Cuts at: 0 104 488 569 2257 3576 4020
Size: 104 384 81 1688 1319 444
Fragments arranged by size:
1688 1319 444 384 104 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 919 3557 4020
Size: 919 2638 463
Fragments arranged by size:
2638 919 463
BtsI GCAGTG_nn'
Cuts at: 0 105 976 1419 1716 2334 2948 3067 3205
Size: 105 871 443 297 618 614 119 138
Cuts at: 3205 3217 3758 3827 3855 4020
Size: 12 541 69 28 165
Fragments arranged by size:
871 618 614 541 443 297 165 138
119 105 69 28 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2223 2258 4020
Size: 2223 35 1762
Fragments arranged by size:
2223 1762 35
DraIII CAC_nnn'GTG
Cuts at: 0 3606 4020
Size: 3606 414
EaeI y'GGCC_r
Cuts at: 0 43 497 572 1706 1818 2387 2647 2701
Size: 43 454 75 1134 112 569 260 54
Cuts at: 2701 2945 3194 3567 3852 4020
Size: 244 249 373 285 168
Fragments arranged by size:
1134 569 454 373 285 260 249 244
168 112 75 54 43
EagI C'GGCC_G
Cuts at: 0 43 2701 3567 4020
Size: 43 2658 866 453
Fragments arranged by size:
2658 866 453 43
EarI CTCTTCn'nnn_
Cuts at: 0 737 811 1493 1499 1541 2905 3238 3266
Size: 737 74 682 6 42 1364 333 28
Cuts at: 3266 3926 4020
Size: 660 94
Fragments arranged by size:
1364 737 682 660 333 94 74 42
28 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1687 2223 2776 3453 3704 4020
Size: 1687 536 553 677 251 316
Fragments arranged by size:
1687 677 553 536 316 251
EcoICRI GAG'CTC
Cuts at: 0 2727 3519 4020
Size: 2727 792 501
Fragments arranged by size:
2727 792 501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 409 694 697 829 934 1300 1496
Size: 177 232 285 3 132 105 366 196
Cuts at: 1496 1591 1779 1943 1958 2005 2314 2547 2610
Size: 95 188 164 15 47 309 233 63
Cuts at: 2610 2654 2780 2929 3299 3480 3489 3796 3859
Size: 44 126 149 370 181 9 307 63
Cuts at: 3859 4020
Size: 161
Fragments arranged by size:
370 366 309 307 285 233 232 196
188 181 177 164 161 149 132 126
105 95 63 63 47 44 15 9
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 1876 4020
Size: 22 1854 2144
Fragments arranged by size:
2144 1854 22
EcoO109I rG'GnC_Cy
Cuts at: 0 217 218 253 330 880 953 1458 1575
Size: 217 1 35 77 550 73 505 117
Cuts at: 1575 1936 2010 2073 2082 2310 2715 2757 3811
Size: 361 74 63 9 228 405 42 1054
Cuts at: 3811 3945 4020
Size: 134 75
Fragments arranged by size:
1054 550 505 405 361 228 217 134
117 77 75 74 73 63 42 35
9 1
EcoRV GAT'ATC
Cuts at: 0 534 4020
Size: 534 3486
FseI GG_CCGG'CC
Cuts at: 0 3459 3573 4020
Size: 3459 114 447
Fragments arranged by size:
3459 447 114
FspI TGC'GCA
Cuts at: 0 337 943 4020
Size: 337 606 3077
Fragments arranged by size:
3077 606 337
HaeII r_GCGC'y
Cuts at: 0 410 1335 1763 4020
Size: 410 925 428 2257
Fragments arranged by size:
2257 925 428 410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 841 873 1294 1326 1954 1986 2221 2253
Size: 841 32 421 32 628 32 235 32
Cuts at: 2253 4020
Size: 1767
Fragments arranged by size:
1767 841 628 421 235 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3591 4020
Size: 3591 429
KasI G'GCGC_C
Cuts at: 0 1331 4020
Size: 1331 2689
KpnI G_GTAC'C
Cuts at: 0 1260 4020
Size: 1260 2760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1852 4020
Size: 1852 2168
MscI TGG'CCA
Cuts at: 0 499 2389 3196 3854 4020
Size: 499 1890 807 658 166
Fragments arranged by size:
1890 807 658 499 166
MslI CAynn'nnrTG
Cuts at: 0 26 734 1318 2327 2377 2552 3210 4020
Size: 26 708 584 1009 50 175 658 810
Fragments arranged by size:
1009 810 708 658 584 175 50 26
MspA1I CmG'CkG
Cuts at: 0 200 247 473 503 1262 2059 2259 2348
Size: 200 47 226 30 759 797 200 89
Cuts at: 2348 3147 3342 3500 3578 3653 4020
Size: 799 195 158 78 75 367
Fragments arranged by size:
799 797 759 367 226 200 200 195
158 89 78 75 47 30
NaeI GCC'GGC
Cuts at: 0 166 1336 3457 3571 4020
Size: 166 1170 2121 114 449
Fragments arranged by size:
2121 1170 449 166 114
NarI GG'CG_CC
Cuts at: 0 1332 4020
Size: 1332 2688
NcoI C'CATG_G
Cuts at: 0 104 4020
Size: 104 3916
NgoMIV G'CCGG_C
Cuts at: 0 164 1334 3455 3569 4020
Size: 164 1170 2121 114 451
Fragments arranged by size:
2121 1170 451 164 114
NsiI A_TGCA'T
Cuts at: 0 592 4020
Size: 592 3428
NspI r_CATG'y
Cuts at: 0 321 594 1408 4020
Size: 321 273 814 2612
Fragments arranged by size:
2612 814 321 273
PasI CC'CwG_GG
Cuts at: 0 2845 3048 3709 4020
Size: 2845 203 661 311
Fragments arranged by size:
2845 661 311 203
PflMI CCAn_nnn'nTGG
Cuts at: 0 200 3102 3292 4020
Size: 200 2902 190 728
Fragments arranged by size:
2902 728 200 190
PfoI T'CCnGG_A
Cuts at: 0 713 845 911 1427 1795 1982 2906 4020
Size: 713 132 66 516 368 187 924 1114
Fragments arranged by size:
1114 924 713 516 368 187 132 66
PmlI CAC'GTG
Cuts at: 0 3114 4020
Size: 3114 906
PpuMI rG'GwC_Cy
Cuts at: 0 330 953 1575 2073 2757 3811 3945 4020
Size: 330 623 622 498 684 1054 134 75
Fragments arranged by size:
1054 684 623 622 498 330 134 75
PspOMI G'GGCC_C
Cuts at: 0 217 880 1936 2714 4020
Size: 217 663 1056 778 1306
Fragments arranged by size:
1306 1056 778 663 217
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3124 3156 4020
Size: 3124 32 864
Fragments arranged by size:
3124 864 32
PstI C_TGCA'G
Cuts at: 0 1267 1466 3212 3765 3834 3915 4020
Size: 1267 199 1746 553 69 81 105
Fragments arranged by size:
1746 1267 553 199 105 81 69
PvuII CAG'CTG
Cuts at: 0 200 473 503 2059 2348 3147 3342 3500
Size: 200 273 30 1556 289 799 195 158
Cuts at: 3500 4020
Size: 520
Fragments arranged by size:
1556 799 520 289 273 200 195 158
30
RsrII CG'GwC_CG
Cuts at: 0 837 4020
Size: 837 3183
SacI G_AGCT'C
Cuts at: 0 2729 3521 4020
Size: 2729 792 499
Fragments arranged by size:
2729 792 499
SacII CC_GC'GG
Cuts at: 0 2260 3579 4020
Size: 2260 1319 441
Fragments arranged by size:
2260 1319 441
SanDI GG'GwC_CC
Cuts at: 0 2757 3811 3945 4020
Size: 2757 1054 134 75
Fragments arranged by size:
2757 1054 134 75
SapI GCTCTTCn'nnn_
Cuts at: 0 1541 3238 4020
Size: 1541 1697 782
Fragments arranged by size:
1697 1541 782
ScaI AGT'ACT
Cuts at: 0 1836 4020
Size: 1836 2184
SfcI C'TryA_G
Cuts at: 0 640 1263 1462 3208 3265 3761 3830 3911
Size: 640 623 199 1746 57 496 69 81
Cuts at: 3911 4020
Size: 109
Fragments arranged by size:
1746 640 623 496 199 109 81 69
57
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3001 4020
Size: 3001 1019
SfoI GGC'GCC
Cuts at: 0 1333 4020
Size: 1333 2687
SgrAI Cr'CCGG_yG
Cuts at: 0 3870 4020
Size: 3870 150
SmaI CCC'GGG
Cuts at: 0 381 1756 3007 4020
Size: 381 1375 1251 1013
Fragments arranged by size:
1375 1251 1013 381
SmlI C'TyrA_G
Cuts at: 0 694 2077 4020
Size: 694 1383 1943
Fragments arranged by size:
1943 1383 694
SnaBI TAC'GTA
Cuts at: 0 324 4020
Size: 324 3696
SphI G_CATG'C
Cuts at: 0 594 1408 4020
Size: 594 814 2612
Fragments arranged by size:
2612 814 594
StuI AGG'CCT
Cuts at: 0 1555 4003 4020
Size: 1555 2448 17
Fragments arranged by size:
2448 1555 17
StyI C'CwwG_G
Cuts at: 0 104 656 1214 4020
Size: 104 552 558 2806
Fragments arranged by size:
2806 558 552 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1102 4020
Size: 1102 2918
TatI w'GTAC_w
Cuts at: 0 1112 1834 3158 4020
Size: 1112 722 1324 862
Fragments arranged by size:
1324 1112 862 722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1956 1988 2888 2920 4020
Size: 1956 32 900 32 1100
Fragments arranged by size:
1956 1100 900 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 507 789 1230 1701 3209 3834 4020
Size: 507 282 441 471 1508 625 186
Fragments arranged by size:
1508 625 507 471 441 282 186
XmaI C'CCGG_G
Cuts at: 0 379 1754 3005 4020
Size: 379 1375 1251 1015
Fragments arranged by size:
1375 1251 1015 379
ZraI GAC'GTC
Cuts at: 0 3510 4020
Size: 3510 510
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AgeI AhdI
AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FseI FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PsrI PstI PvuII RsrII SacI SacII SanDI
SapI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI
SnaBI SphI StuI StyI TaqII TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflIII AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BclI BmgBI BmtI
BsiWI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I
ClaI DraI DrdI EcoRI FalI FspAI HindIII HpaI
MfeI MluI NdeI NheI NotI NruI PacI PciI
PmeI PpiI PshAI PsiI PspXI PvuI SalI SbfI
SexAI SgfI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI