Restriction map for
pF1KSDA0601
(Linear) (Six-base) MAPSORT of: KIAA0601.seq Check: 181 from: 1 to: 2556
KIAA0601.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 2500 2556
Size: 2500 56
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1418 1507 1775 1909 2556
Size: 524 894 89 268 134 647
Fragments arranged by size:
894 647 524 268 134 89
AflII C'TTAA_G
Cuts at: 0 1645 2556
Size: 1645 911
AgeI A'CCGG_T
Cuts at: 0 1148 2556
Size: 1148 1408
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1091 1123 2393 2425 2556
Size: 1091 32 1270 32 131
Fragments arranged by size:
1270 1091 131 32 32
ApaI G_GGCC'C
Cuts at: 0 139 2556
Size: 139 2417
ApoI r'AATT_y
Cuts at: 0 1347 1609 2556
Size: 1347 262 947
Fragments arranged by size:
1347 947 262
AvaI C'yCGr_G
Cuts at: 0 211 2270 2556
Size: 211 2059 286
Fragments arranged by size:
2059 286 211
BamHI G'GATC_C
Cuts at: 0 2013 2556
Size: 2013 543
BanI G'GyrC_C
Cuts at: 0 2362 2556
Size: 2362 194
BanII G_rGCy'C
Cuts at: 0 139 2072 2271 2278 2556
Size: 139 1933 199 7 278
Fragments arranged by size:
1933 278 199 139 7
BbvCI CC'TCA_GC
Cuts at: 0 2222 2556
Size: 2222 334
BglI GCCn_nnn'nGGC
Cuts at: 0 31 134 2556
Size: 31 103 2422
Fragments arranged by size:
2422 103 31
BlpI GC'TnA_GC
Cuts at: 0 2330 2556
Size: 2330 226
Bme1580I G_kGCm'C
Cuts at: 0 139 2367 2556
Size: 139 2228 189
Fragments arranged by size:
2228 189 139
BmrI ACTGGGnnnn_n'
Cuts at: 0 1662 2556
Size: 1662 894
Bpu10I CC'TnA_GC
Cuts at: 0 2222 2556
Size: 2222 334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 554 2556
Size: 452 102 2002
Fragments arranged by size:
2002 452 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 867 2556
Size: 867 1689
BsaBI GATnn'nnATC
Cuts at: 0 1556 2556
Size: 1556 1000
BsaHI Gr'CG_yC
Cuts at: 0 321 2556
Size: 321 2235
BsaWI w'CCGG_w
Cuts at: 0 312 557 1148 2556
Size: 312 245 591 1408
Fragments arranged by size:
1408 591 312 245
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 980 1010 2556
Size: 980 30 1546
Fragments arranged by size:
1546 980 30
BseYI C'CCAG_C
Cuts at: 0 138 2421 2556
Size: 138 2283 135
Fragments arranged by size:
2283 138 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2234 2519 2556
Size: 2234 285 37
Fragments arranged by size:
2234 285 37
BsiHKAI G_wGCw'C
Cuts at: 0 2072 2442 2556
Size: 2072 370 114
Fragments arranged by size:
2072 370 114
Bsp1286I G_dGCh'C
Cuts at: 0 139 2072 2271 2278 2367 2442 2556
Size: 139 1933 199 7 89 75 114
Fragments arranged by size:
1933 199 139 114 89 75 7
BspEI T'CCGG_A
Cuts at: 0 312 2556
Size: 312 2244
BspHI T'CATG_A
Cuts at: 0 552 2556
Size: 552 2004
BsrBI CCG'CTC
Cuts at: 0 1823 2556
Size: 1823 733
BsrFI r'CCGG_y
Cuts at: 0 253 332 1148 2556
Size: 253 79 816 1408
Fragments arranged by size:
1408 816 253 79
BssSI C'ACGA_G
Cuts at: 0 1713 1827 2556
Size: 1713 114 729
Fragments arranged by size:
1713 729 114
BstYI r'GATC_y
Cuts at: 0 1455 2013 2556
Size: 1455 558 543
Fragments arranged by size:
1455 558 543
BstZ17I GTA'TAC
Cuts at: 0 2501 2556
Size: 2501 55
Bsu36I CC'TnA_GG
Cuts at: 0 248 479 2556
Size: 248 231 2077
Fragments arranged by size:
2077 248 231
BtgI C'CryG_G
Cuts at: 0 26 122 196 287 992 2556
Size: 26 96 74 91 705 1564
Fragments arranged by size:
1564 705 96 91 74 26
BtsI GCAGTG_nn'
Cuts at: 0 649 1772 2217 2556
Size: 649 1123 445 339
Fragments arranged by size:
1123 649 445 339
DraIII CAC_nnn'GTG
Cuts at: 0 2527 2556
Size: 2527 29
DrdI GACnn_nn'nnGTC
Cuts at: 0 1195 2556
Size: 1195 1361
EaeI y'GGCC_r
Cuts at: 0 110 380 1058 1224 2172 2183 2556
Size: 110 270 678 166 948 11 373
Fragments arranged by size:
948 678 373 270 166 110 11
EarI CTCTTCn'nnn_
Cuts at: 0 410 2077 2083 2392 2556
Size: 410 1667 6 309 164
Fragments arranged by size:
1667 410 309 164 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 238 2556
Size: 238 2318
EcoICRI GAG'CTC
Cuts at: 0 2070 2556
Size: 2070 486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1418 1507 1775 1909 2556
Size: 524 894 89 268 134 647
Fragments arranged by size:
894 647 524 268 134 89
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1225 1471 1978 2556
Size: 1225 246 507 578
Fragments arranged by size:
1225 578 507 246
EcoO109I rG'GnC_Cy
Cuts at: 0 216 244 2556
Size: 216 28 2312
Fragments arranged by size:
2312 216 28
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1348 1537 2556
Size: 1348 189 1019
Fragments arranged by size:
1348 1019 189
FseI GG_CCGG'CC
Cuts at: 0 257 2556
Size: 257 2299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 552 584 2556
Size: 85 32 435 32 1972
Fragments arranged by size:
1972 435 85 32 32
HindIII A'AGCT_T
Cuts at: 0 375 448 2556
Size: 375 73 2108
Fragments arranged by size:
2108 375 73
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 644 729 1511 2556
Size: 304 340 85 782 1045
Fragments arranged by size:
1045 782 340 304 85
MscI TGG'CCA
Cuts at: 0 382 1060 1226 2185 2556
Size: 382 678 166 959 371
Fragments arranged by size:
959 678 382 371 166
MspA1I CmG'CkG
Cuts at: 0 28 46 124 198 684 2556
Size: 28 18 78 74 486 1872
Fragments arranged by size:
1872 486 78 74 28 18
NaeI GCC'GGC
Cuts at: 0 255 334 2556
Size: 255 79 2222
Fragments arranged by size:
2222 255 79
NcoI C'CATG_G
Cuts at: 0 287 992 2556
Size: 287 705 1564
Fragments arranged by size:
1564 705 287
NgoMIV G'CCGG_C
Cuts at: 0 253 332 2556
Size: 253 79 2224
Fragments arranged by size:
2224 253 79
NspI r_CATG'y
Cuts at: 0 1267 2041 2556
Size: 1267 774 515
Fragments arranged by size:
1267 774 515
PasI CC'CwG_GG
Cuts at: 0 73 1878 2264 2556
Size: 73 1805 386 292
Fragments arranged by size:
1805 386 292 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 1233 1959 2487 2556
Size: 1233 726 528 69
Fragments arranged by size:
1233 726 528 69
PsiI TTA'TAA
Cuts at: 0 725 1847 2556
Size: 725 1122 709
Fragments arranged by size:
1122 725 709
PspOMI G'GGCC_C
Cuts at: 0 135 2556
Size: 135 2421
PstI C_TGCA'G
Cuts at: 0 45 656 1950 2298 2556
Size: 45 611 1294 348 258
Fragments arranged by size:
1294 611 348 258 45
PvuII CAG'CTG
Cuts at: 0 684 2556
Size: 684 1872
SacI G_AGCT'C
Cuts at: 0 2072 2556
Size: 2072 484
SacII CC_GC'GG
Cuts at: 0 29 125 199 2556
Size: 29 96 74 2357
Fragments arranged by size:
2357 96 74 29
SapI GCTCTTCn'nnn_
Cuts at: 0 2077 2556
Size: 2077 479
SexAI A'CCwGG_T
Cuts at: 0 2523 2556
Size: 2523 33
SfcI C'TryA_G
Cuts at: 0 41 652 1946 2294 2556
Size: 41 611 1294 348 262
Fragments arranged by size:
1294 611 348 262 41
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 134 2556
Size: 134 2422
SmaI CCC'GGG
Cuts at: 0 213 2556
Size: 213 2343
SmlI C'TyrA_G
Cuts at: 0 467 569 1645 2556
Size: 467 102 1076 911
Fragments arranged by size:
1076 911 467 102
SpeI A'CTAG_T
Cuts at: 0 2115 2556
Size: 2115 441
StuI AGG'CCT
Cuts at: 0 1742 2556
Size: 1742 814
StyI C'CwwG_G
Cuts at: 0 287 992 1221 2556
Size: 287 705 229 1335
Fragments arranged by size:
1335 705 287 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 928 1425 1870 2556
Size: 928 497 445 686
Fragments arranged by size:
928 686 497 445
TatI w'GTAC_w
Cuts at: 0 356 2556
Size: 356 2200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 980 1012 1787 1819 2556
Size: 980 32 775 32 737
Fragments arranged by size:
980 775 737 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 933 2235 2556
Size: 933 1302 321
Fragments arranged by size:
1302 933 321
XmaI C'CCGG_G
Cuts at: 0 211 2556
Size: 211 2345
XmnI GAAnn'nnTTC
Cuts at: 0 1520 2556
Size: 1520 1036
Enzymes that do cut:
AccI AcuI AflII AgeI AloI ApaI ApoI AvaI
BamHI BanI BanII BbvCI BglI BlpI Bme1580I BmrI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrFI BssSI
BstYI BstZ17I Bsu36I BtgI BtsI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
Hin4I HindIII MmeI MscI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PsiI PspOMI PstI PvuII SacI
SacII SapI SexAI SfcI SfiI SmaI SmlI SpeI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AhdI AleI
AlfI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BplI BpmI BsaAI BseRI BsiEI BsiWI BsmI
BsmBI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstXI BtgZI ClaI CspCI DraI EagI EcoRI EcoRV
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MslI NarI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SfoI SgfI SgrAI SnaBI SphI SrfI SspI SwaI
Tth111I XbaI XhoI ZraI