Restriction map for
pF1KA0602
(Linear) (Six-base) MAPSORT of: KIAA0602.seq Check: 8268 from: 1 to: 2712
KIAA0602.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2277 2712
Size: 2277 435
AatII G_ACGT'C
Cuts at: 0 730 1532 1583 2627 2712
Size: 730 802 51 1044 85
Fragments arranged by size:
1044 802 730 85 51
Acc65I G'GTAC_C
Cuts at: 0 1799 2712
Size: 1799 913
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 316 461 1578 2712
Size: 157 159 145 1117 1134
Fragments arranged by size:
1134 1117 159 157 145
AfeI AGC'GCT
Cuts at: 0 358 770 1372 2712
Size: 358 412 602 1340
Fragments arranged by size:
1340 602 412 358
AhdI GACnn_n'nnGTC
Cuts at: 0 1911 2043 2520 2712
Size: 1911 132 477 192
Fragments arranged by size:
1911 477 192 132
AleI CACnn'nnGTG
Cuts at: 0 55 2712
Size: 55 2657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 839 2712
Size: 839 1873
AlwNI CAG_nnn'CTG
Cuts at: 0 456 1845 2712
Size: 456 1389 867
Fragments arranged by size:
1389 867 456
ApaLI G'TGCA_C
Cuts at: 0 1560 2376 2712
Size: 1560 816 336
Fragments arranged by size:
1560 816 336
AscI GG'CGCG_CC
Cuts at: 0 32 2712
Size: 32 2680
AvaI C'yCGr_G
Cuts at: 0 838 984 1060 1137 1171 2142 2712
Size: 838 146 76 77 34 971 570
Fragments arranged by size:
971 838 570 146 77 76 34
AvrII C'CTAG_G
Cuts at: 0 1803 2712
Size: 1803 909
BanI G'GyrC_C
Cuts at: 0 1799 2266 2712
Size: 1799 467 446
Fragments arranged by size:
1799 467 446
BanII G_rGCy'C
Cuts at: 0 211 406 991 1040 1783 2149 2712
Size: 211 195 585 49 743 366 563
Fragments arranged by size:
743 585 563 366 211 195 49
BbeI G_GCGC'C
Cuts at: 0 2270 2712
Size: 2270 442
BbsI GAAGACnn'nnnn_
Cuts at: 0 144 551 1072 1429 2712
Size: 144 407 521 357 1283
Fragments arranged by size:
1283 521 407 357 144
BbvCI CC'TCA_GC
Cuts at: 0 419 934 1178 2712
Size: 419 515 244 1534
Fragments arranged by size:
1534 515 419 244
BciVI GTATCCnnnnn_n'
Cuts at: 0 308 1607 2712
Size: 308 1299 1105
Fragments arranged by size:
1299 1105 308
BglI GCCn_nnn'nGGC
Cuts at: 0 20 29 1295 2197 2712
Size: 20 9 1266 902 515
Fragments arranged by size:
1266 902 515 20 9
BglII A'GATC_T
Cuts at: 0 503 2712
Size: 503 2209
BlpI GC'TnA_GC
Cuts at: 0 457 2712
Size: 457 2255
Bme1580I G_kGCm'C
Cuts at: 0 62 116 1061 1564 1953 2380 2712
Size: 62 54 945 503 389 427 332
Fragments arranged by size:
945 503 427 389 332 62 54
BmgBI CAC'GTC
Cuts at: 0 684 1056 2517 2712
Size: 684 372 1461 195
Fragments arranged by size:
1461 684 372 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 245 1786 2712
Size: 245 1541 926
Fragments arranged by size:
1541 926 245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 195 633 2121 2712
Size: 34 161 438 1488 591
Fragments arranged by size:
1488 591 438 161 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 184 1872 1894 2377 2712
Size: 81 103 1688 22 483 335
Fragments arranged by size:
1688 483 335 103 81 22
Bpu10I CC'TnA_GC
Cuts at: 0 419 934 1178 2029 2712
Size: 419 515 244 851 683
Fragments arranged by size:
851 683 515 419 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2356 2712
Size: 2356 356
BsaI GGTCTCn'nnnn_
Cuts at: 0 1850 2712
Size: 1850 862
BsaAI yAC'GTr
Cuts at: 0 1565 2664 2712
Size: 1565 1099 48
Fragments arranged by size:
1565 1099 48
BsaBI GATnn'nnATC
Cuts at: 0 508 2712
Size: 508 2204
BsaHI Gr'CG_yC
Cuts at: 0 644 727 1382 1529 1580 2267 2333 2624
Size: 644 83 655 147 51 687 66 291
Cuts at: 2624 2712
Size: 88
Fragments arranged by size:
687 655 644 291 147 88 83 66
51
BsaWI w'CCGG_w
Cuts at: 0 2384 2712
Size: 2384 328
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1554 1584 1708 1738 1792 1822 2712
Size: 1554 30 124 30 54 30 890
Fragments arranged by size:
1554 890 124 54 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 609 613 616 862 2151 2208 2712
Size: 609 4 3 246 1289 57 504
Fragments arranged by size:
1289 609 504 246 57 4 3
BseYI C'CCAG_C
Cuts at: 0 103 892 1219 1640 2251 2410 2712
Size: 103 789 327 421 611 159 302
Fragments arranged by size:
789 611 421 327 302 159 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 670 1423 2547 2637 2712
Size: 144 526 753 1124 90 75
Fragments arranged by size:
1124 753 526 144 90 75
BsiEI CG_ry'CG
Cuts at: 0 500 2712
Size: 500 2212
BsiHKAI G_wGCw'C
Cuts at: 0 833 890 991 1148 1306 1564 1570 2380
Size: 833 57 101 157 158 258 6 810
Cuts at: 2380 2712
Size: 332
Fragments arranged by size:
833 810 332 258 158 157 101 57
6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1611 2712
Size: 1611 1101
Bsp1286I G_dGCh'C
Cuts at: 0 62 116 211 406 833 890 991 1040
Size: 62 54 95 195 427 57 101 49
Cuts at: 1040 1061 1148 1306 1564 1570 1783 1953 2149
Size: 21 87 158 258 6 213 170 196
Cuts at: 2149 2380 2712
Size: 231 332
Fragments arranged by size:
427 332 258 231 213 196 195 170
158 101 95 87 62 57 54 49
21 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2277 2357 2712
Size: 2277 80 355
Fragments arranged by size:
2277 355 80
BsrBI CCG'CTC
Cuts at: 0 1320 1347 2234 2712
Size: 1320 27 887 478
Fragments arranged by size:
1320 887 478 27
BsrFI r'CCGG_y
Cuts at: 0 562 2552 2712
Size: 562 1990 160
Fragments arranged by size:
1990 562 160
BssHII G'CGCG_C
Cuts at: 0 32 41 2712
Size: 32 9 2671
Fragments arranged by size:
2671 32 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2185 2314 2586 2712
Size: 2185 129 272 126
Fragments arranged by size:
2185 272 129 126
BstEII G'GTnAC_C
Cuts at: 0 2457 2712
Size: 2457 255
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1359 2454 2712
Size: 414 945 1095 258
Fragments arranged by size:
1095 945 414 258
BstYI r'GATC_y
Cuts at: 0 503 1412 2712
Size: 503 909 1300
Fragments arranged by size:
1300 909 503
Bsu36I CC'TnA_GG
Cuts at: 0 1160 2712
Size: 1160 1552
BtgI C'CryG_G
Cuts at: 0 182 398 581 1553 1667 1790 1811 2453
Size: 182 216 183 972 114 123 21 642
Cuts at: 2453 2712
Size: 259
Fragments arranged by size:
972 642 259 216 183 182 123 114
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1917 1950 2712
Size: 1917 33 762
Fragments arranged by size:
1917 762 33
BtsI GCAGTG_nn'
Cuts at: 0 2564 2656 2712
Size: 2564 92 56
Fragments arranged by size:
2564 92 56
ClaI AT'CG_AT
Cuts at: 0 712 2712
Size: 712 2000
DraIII CAC_nnn'GTG
Cuts at: 0 1791 2712
Size: 1791 921
DrdI GACnn_nn'nnGTC
Cuts at: 0 928 2353 2712
Size: 928 1425 359
Fragments arranged by size:
1425 928 359
EaeI y'GGCC_r
Cuts at: 0 2 395 447 497 958 1287 1793 2108
Size: 2 393 52 50 461 329 506 315
Cuts at: 2108 2189 2642 2712
Size: 81 453 70
Fragments arranged by size:
506 461 453 393 329 315 81 70
52 50 2
EagI C'GGCC_G
Cuts at: 0 497 2712
Size: 497 2215
EarI CTCTTCn'nnn_
Cuts at: 0 307 1990 2036 2320 2712
Size: 307 1683 46 284 392
Fragments arranged by size:
1683 392 307 284 46
EcoICRI GAG'CTC
Cuts at: 0 989 2712
Size: 989 1723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 157 184 316 461 1578 1872 1894
Size: 81 76 27 132 145 1117 294 22
Cuts at: 1894 2377 2712
Size: 483 335
Fragments arranged by size:
1117 483 335 294 145 132 81 76
27 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1263 2712
Size: 1263 1449
EcoO109I rG'GnC_Cy
Cuts at: 0 483 801 819 852 1842 2346 2595 2712
Size: 483 318 18 33 990 504 249 117
Fragments arranged by size:
990 504 483 318 249 117 33 18
FseI GG_CCGG'CC
Cuts at: 0 566 2712
Size: 566 2146
HaeII r_GCGC'y
Cuts at: 0 360 772 1022 1374 2270 2712
Size: 360 412 250 352 896 442
Fragments arranged by size:
896 442 412 360 352 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 1090 1122 1708 1740 1897 1929
Size: 577 32 481 32 586 32 157 32
Cuts at: 1929 2029 2061 2506 2538 2712
Size: 100 32 445 32 174
Fragments arranged by size:
586 577 481 445 174 157 100 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1494 2712
Size: 1494 1218
HindIII A'AGCT_T
Cuts at: 0 328 2712
Size: 328 2384
KasI G'GCGC_C
Cuts at: 0 2266 2712
Size: 2266 446
KpnI G_GTAC'C
Cuts at: 0 1803 2712
Size: 1803 909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 1550 2067 2712
Size: 818 732 517 645
Fragments arranged by size:
818 732 645 517
MscI TGG'CCA
Cuts at: 0 449 1795 2712
Size: 449 1346 917
Fragments arranged by size:
1346 917 449
MslI CAynn'nnrTG
Cuts at: 0 55 412 2446 2452 2712
Size: 55 357 2034 6 260
Fragments arranged by size:
2034 357 260 55 6
MspA1I CmG'CkG
Cuts at: 0 107 400 705 902 1223 1407 1722 2417
Size: 107 293 305 197 321 184 315 695
Cuts at: 2417 2640 2712
Size: 223 72
Fragments arranged by size:
695 321 315 305 293 223 197 184
107 72
NaeI GCC'GGC
Cuts at: 0 564 2554 2712
Size: 564 1990 158
Fragments arranged by size:
1990 564 158
NarI GG'CG_CC
Cuts at: 0 2267 2712
Size: 2267 445
NgoMIV G'CCGG_C
Cuts at: 0 562 2552 2712
Size: 562 1990 160
Fragments arranged by size:
1990 562 160
NspI r_CATG'y
Cuts at: 0 1758 2589 2712
Size: 1758 831 123
Fragments arranged by size:
1758 831 123
PasI CC'CwG_GG
Cuts at: 0 1077 2712
Size: 1077 1635
PflMI CCAn_nnn'nTGG
Cuts at: 0 1791 1845 2712
Size: 1791 54 867
Fragments arranged by size:
1791 867 54
PfoI T'CCnGG_A
Cuts at: 0 803 1838 2712
Size: 803 1035 874
Fragments arranged by size:
1035 874 803
PmlI CAC'GTG
Cuts at: 0 1565 2664 2712
Size: 1565 1099 48
Fragments arranged by size:
1565 1099 48
PpuMI rG'GwC_Cy
Cuts at: 0 801 819 852 1842 2346 2595 2712
Size: 801 18 33 990 504 249 117
Fragments arranged by size:
990 801 504 249 117 33 18
PshAI GACnn'nnGTC
Cuts at: 0 2455 2712
Size: 2455 257
PspXI vC'TCGA_Gb
Cuts at: 0 984 2712
Size: 984 1728
PstI C_TGCA'G
Cuts at: 0 347 469 1412 1538 2288 2430 2712
Size: 347 122 943 126 750 142 282
Fragments arranged by size:
943 750 347 282 142 126 122
PvuII CAG'CTG
Cuts at: 0 107 1407 1722 2640 2712
Size: 107 1300 315 918 72
Fragments arranged by size:
1300 918 315 107 72
SacI G_AGCT'C
Cuts at: 0 991 2712
Size: 991 1721
SacII CC_GC'GG
Cuts at: 0 401 2712
Size: 401 2311
SexAI A'CCwGG_T
Cuts at: 0 1264 2712
Size: 1264 1448
SfcI C'TryA_G
Cuts at: 0 343 465 1396 1408 1534 2284 2426 2712
Size: 343 122 931 12 126 750 142 286
Fragments arranged by size:
931 750 343 286 142 126 122 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 20 1295 2197 2712
Size: 20 1275 902 515
Fragments arranged by size:
1275 902 515 20
SfoI GGC'GCC
Cuts at: 0 2268 2712
Size: 2268 444
SmlI C'TyrA_G
Cuts at: 0 984 2371 2712
Size: 984 1387 341
Fragments arranged by size:
1387 984 341
StuI AGG'CCT
Cuts at: 0 974 1183 2200 2240 2712
Size: 974 209 1017 40 472
Fragments arranged by size:
1017 974 472 209 40
StyI C'CwwG_G
Cuts at: 0 569 1803 2192 2462 2699 2712
Size: 569 1234 389 270 237 13
Fragments arranged by size:
1234 569 389 270 237 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2220 2712
Size: 2220 492
TatI w'GTAC_w
Cuts at: 0 1928 2712
Size: 1928 784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1554 1586 2712
Size: 1554 32 1126
Fragments arranged by size:
1554 1126 32
Tth111I GACn'n_nGTC
Cuts at: 0 799 928 1071 1102 1242 2712
Size: 799 129 143 31 140 1470
Fragments arranged by size:
1470 799 143 140 129 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 258 2712
Size: 84 174 2454
Fragments arranged by size:
2454 174 84
XhoI C'TCGA_G
Cuts at: 0 984 2712
Size: 984 1728
XmnI GAAnn'nnTTC
Cuts at: 0 70 2671 2712
Size: 70 2601 41
Fragments arranged by size:
2601 70 41
ZraI GAC'GTC
Cuts at: 0 728 1530 1581 2625 2712
Size: 728 802 51 1044 87
Fragments arranged by size:
1044 802 728 87 51
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AhdI AleI AlfI
AlwNI ApaLI AscI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PshAI PspXI PstI PvuII
SacI SacII SexAI SfcI SfiI SfoI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AloI ApaI ApoI
AseI AsiSI BaeI BamHI BcgI BclI BfrBI BmtI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstZ17I CspCI DraI EciI EcoRI EcoRV FalI FspI
FspAI HpaI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PsiI PspOMI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XmaI