Restriction map for
pF1KA0603
(Linear) (Six-base) MAPSORT of: KIAA0603.seq Check: 4615 from: 1 to: 3897
KIAA0603.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 2166 2769 3897
Size: 2166 603 1128
Fragments arranged by size:
2166 1128 603
AclI AA'CG_TT
Cuts at: 0 1873 3897
Size: 1873 2024
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1293 1781 2195 3316 3362 3544 3897
Size: 730 563 488 414 1121 46 182 353
Fragments arranged by size:
1121 730 563 488 414 353 182 46
AflIII A'CryG_T
Cuts at: 0 1584 1922 3897
Size: 1584 338 1975
Fragments arranged by size:
1975 1584 338
AhdI GACnn_n'nnGTC
Cuts at: 0 450 3147 3897
Size: 450 2697 750
Fragments arranged by size:
2697 750 450
AleI CACnn'nnGTG
Cuts at: 0 961 3068 3897
Size: 961 2107 829
Fragments arranged by size:
2107 961 829
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2897 3536 3897
Size: 2897 639 361
Fragments arranged by size:
2897 639 361
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 16 48 3897
Size: 16 32 3849
Fragments arranged by size:
3849 32 16
AlwNI CAG_nnn'CTG
Cuts at: 0 708 1222 2793 3897
Size: 708 514 1571 1104
Fragments arranged by size:
1571 1104 708 514
ApaLI G'TGCA_C
Cuts at: 0 1588 3897
Size: 1588 2309
ApoI r'AATT_y
Cuts at: 0 1140 1962 2157 2780 3097 3897
Size: 1140 822 195 623 317 800
Fragments arranged by size:
1140 822 800 623 317 195
AvaI C'yCGr_G
Cuts at: 0 52 82 205 236 303 448 778 875
Size: 52 30 123 31 67 145 330 97
Cuts at: 875 3897
Size: 3022
Fragments arranged by size:
3022 330 145 123 97 67 52 31
30
BanI G'GyrC_C
Cuts at: 0 225 774 817 3897
Size: 225 549 43 3080
Fragments arranged by size:
3080 549 225 43
BanII G_rGCy'C
Cuts at: 0 53 1983 2017 3897
Size: 53 1930 34 1880
Fragments arranged by size:
1930 1880 53 34
BbeI G_GCGC'C
Cuts at: 0 229 3897
Size: 229 3668
BbsI GAAGACnn'nnnn_
Cuts at: 0 1585 1917 2077 2086 3897
Size: 1585 332 160 9 1811
Fragments arranged by size:
1811 1585 332 160 9
BbvCI CC'TCA_GC
Cuts at: 0 1860 2005 2364 3897
Size: 1860 145 359 1533
Fragments arranged by size:
1860 1533 359 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 950 3897
Size: 916 34 2947
Fragments arranged by size:
2947 916 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3013 3897
Size: 3013 884
BclI T'GATC_A
Cuts at: 0 425 3897
Size: 425 3472
BglII A'GATC_T
Cuts at: 0 3204 3897
Size: 3204 693
BlpI GC'TnA_GC
Cuts at: 0 71 256 2885 3897
Size: 71 185 2629 1012
Fragments arranged by size:
2629 1012 185 71
Bme1580I G_kGCm'C
Cuts at: 0 313 779 1592 1920 3897
Size: 313 466 813 328 1977
Fragments arranged by size:
1977 813 466 328 313
BmgBI CAC'GTC
Cuts at: 0 1035 3897
Size: 1035 2862
BmrI ACTGGGnnnn_n'
Cuts at: 0 1022 3236 3897
Size: 1022 2214 661
Fragments arranged by size:
2214 1022 661
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2626 3897
Size: 2626 1271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1199 2001 2064 2596 3068 3628 3897
Size: 67 1132 802 63 532 472 560 269
Fragments arranged by size:
1132 802 560 532 472 269 67 63
Bpu10I CC'TnA_GC
Cuts at: 0 890 1860 1982 2005 2364 3897
Size: 890 970 122 23 359 1533
Fragments arranged by size:
1533 970 890 359 122 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 1710 3897
Size: 639 1071 2187
Fragments arranged by size:
2187 1071 639
BsaI GGTCTCn'nnnn_
Cuts at: 0 944 1414 3718 3842 3897
Size: 944 470 2304 124 55
Fragments arranged by size:
2304 944 470 124 55
BsaAI yAC'GTr
Cuts at: 0 1587 3897
Size: 1587 2310
BsaHI Gr'CG_yC
Cuts at: 0 62 226 581 3897
Size: 62 164 355 3316
Fragments arranged by size:
3316 355 164 62
BsaWI w'CCGG_w
Cuts at: 0 735 3746 3797 3897
Size: 735 3011 51 100
Fragments arranged by size:
3011 735 100 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 922 952 3897
Size: 922 30 2945
Fragments arranged by size:
2945 922 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 820 1146 2288 2452 2532 2789 3897
Size: 820 326 1142 164 80 257 1108
Fragments arranged by size:
1142 1108 820 326 257 164 80
BseYI C'CCAG_C
Cuts at: 0 10 295 490 562 998 3897
Size: 10 285 195 72 436 2899
Fragments arranged by size:
2899 436 285 195 72 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 966 2049 3046 3897
Size: 676 290 1083 997 851
Fragments arranged by size:
1083 997 851 676 290
BsiHKAI G_wGCw'C
Cuts at: 0 257 362 1592 2017 3897
Size: 257 105 1230 425 1880
Fragments arranged by size:
1880 1230 425 257 105
BsmI GAATG_Cn'
Cuts at: 0 17 3897
Size: 17 3880
BsmBI CGTCTCn'nnnn_
Cuts at: 0 69 3897
Size: 69 3828
Bsp1286I G_dGCh'C
Cuts at: 0 53 257 313 362 779 1592 1920 1983
Size: 53 204 56 49 417 813 328 63
Cuts at: 1983 2017 3897
Size: 34 1880
Fragments arranged by size:
1880 813 417 328 204 63 56 53
49 34
BspEI T'CCGG_A
Cuts at: 0 735 3746 3797 3897
Size: 735 3011 51 100
Fragments arranged by size:
3011 735 100 51
BspHI T'CATG_A
Cuts at: 0 3189 3897
Size: 3189 708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 849 2263 3897
Size: 849 1414 1634
Fragments arranged by size:
1634 1414 849
BsrBI CCG'CTC
Cuts at: 0 894 3897
Size: 894 3003
BsrDI GCAATG_nn'
Cuts at: 0 2268 2375 3897
Size: 2268 107 1522
Fragments arranged by size:
2268 1522 107
BsrFI r'CCGG_y
Cuts at: 0 228 958 3897
Size: 228 730 2939
Fragments arranged by size:
2939 730 228
BsrGI T'GTAC_A
Cuts at: 0 2057 3897
Size: 2057 1840
BssHII G'CGCG_C
Cuts at: 0 260 3897
Size: 260 3637
BssSI C'ACGA_G
Cuts at: 0 979 2902 3897
Size: 979 1923 995
Fragments arranged by size:
1923 995 979
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1380 3897
Size: 1380 2517
BstBI TT'CG_AA
Cuts at: 0 605 1908 2562 3897
Size: 605 1303 654 1335
Fragments arranged by size:
1335 1303 654 605
BstEII G'GTnAC_C
Cuts at: 0 627 3897
Size: 627 3270
BstYI r'GATC_y
Cuts at: 0 182 713 1693 2306 3204 3897
Size: 182 531 980 613 898 693
Fragments arranged by size:
980 898 693 613 531 182
Bsu36I CC'TnA_GG
Cuts at: 0 1552 2332 3897
Size: 1552 780 1565
Fragments arranged by size:
1565 1552 780
BtgI C'CryG_G
Cuts at: 0 2432 3812 3897
Size: 2432 1380 85
Fragments arranged by size:
2432 1380 85
BtsI GCAGTG_nn'
Cuts at: 0 957 1656 2123 2204 2879 3897
Size: 957 699 467 81 675 1018
Fragments arranged by size:
1018 957 699 675 467 81
ClaI AT'CG_AT
Cuts at: 0 665 3897
Size: 665 3232
DraI TTT'AAA
Cuts at: 0 1146 3897
Size: 1146 2751
EaeI y'GGCC_r
Cuts at: 0 176 323 539 3897
Size: 176 147 216 3358
Fragments arranged by size:
3358 216 176 147
EarI CTCTTCn'nnn_
Cuts at: 0 1078 1877 2747 3068 3897
Size: 1078 799 870 321 829
Fragments arranged by size:
1078 870 829 799 321
EcoICRI GAG'CTC
Cuts at: 0 2015 3897
Size: 2015 1882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 730 1199 1293 1781 2001 2064 2195
Size: 67 663 469 94 488 220 63 131
Cuts at: 2195 2596 3068 3316 3362 3544 3628 3897
Size: 401 472 248 46 182 84 269
Fragments arranged by size:
663 488 472 469 401 269 248 220
182 131 94 84 67 63 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 802 3897
Size: 427 375 3095
Fragments arranged by size:
3095 427 375
EcoO109I rG'GnC_Cy
Cuts at: 0 299 648 750 782 1603 2243 3897
Size: 299 349 102 32 821 640 1654
Fragments arranged by size:
1654 821 640 349 299 102 32
EcoRI G'AATT_C
Cuts at: 0 2157 3897
Size: 2157 1740
EcoRV GAT'ATC
Cuts at: 0 1152 3897
Size: 1152 2745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1713 3406 3897
Size: 1713 1693 491
Fragments arranged by size:
1713 1693 491
HaeII r_GCGC'y
Cuts at: 0 229 296 708 3897
Size: 229 67 412 3189
Fragments arranged by size:
3189 412 229 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 436 468 858 890 922 954
Size: 230 32 174 32 390 32 32 32
Cuts at: 954 1105 1137 1141 1173 1392 1424 1879 1911
Size: 151 32 4 32 219 32 455 32
Cuts at: 1911 2696 2728 3133 3165 3897
Size: 785 32 405 32 732
Fragments arranged by size:
785 732 455 405 390 230 219 174
151 32 32 32 32 32 32 32
32 32 32 32 4
HincII GTy'rAC
Cuts at: 0 2770 3897
Size: 2770 1127
HindIII A'AGCT_T
Cuts at: 0 2145 3897
Size: 2145 1752
KasI G'GCGC_C
Cuts at: 0 225 3897
Size: 225 3672
MfeI C'AATT_G
Cuts at: 0 3828 3897
Size: 3828 69
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1808 1916 2452 3897
Size: 1808 108 536 1445
Fragments arranged by size:
1808 1445 536 108
MslI CAynn'nnrTG
Cuts at: 0 961 3068 3524 3897
Size: 961 2107 456 373
Fragments arranged by size:
2107 961 456 373
MspA1I CmG'CkG
Cuts at: 0 14 1560 2448 2967 3174 3814 3897
Size: 14 1546 888 519 207 640 83
Fragments arranged by size:
1546 888 640 519 207 83 14
NaeI GCC'GGC
Cuts at: 0 230 3897
Size: 230 3667
NarI GG'CG_CC
Cuts at: 0 226 3897
Size: 226 3671
NcoI C'CATG_G
Cuts at: 0 2432 3897
Size: 2432 1465
NgoMIV G'CCGG_C
Cuts at: 0 228 3897
Size: 228 3669
NspI r_CATG'y
Cuts at: 0 1183 2058 2657 2896 3523 3897
Size: 1183 875 599 239 627 374
Fragments arranged by size:
1183 875 627 599 374 239
PflMI CCAn_nnn'nTGG
Cuts at: 0 1222 3690 3897
Size: 1222 2468 207
Fragments arranged by size:
2468 1222 207
PmlI CAC'GTG
Cuts at: 0 1587 3897
Size: 1587 2310
PpuMI rG'GwC_Cy
Cuts at: 0 750 782 2243 3897
Size: 750 32 1461 1654
Fragments arranged by size:
1654 1461 750 32
PshAI GACnn'nnGTC
Cuts at: 0 2967 3897
Size: 2967 930
PsiI TTA'TAA
Cuts at: 0 3394 3897
Size: 3394 503
PstI C_TGCA'G
Cuts at: 0 1331 2260 2704 3158 3897
Size: 1331 929 444 454 739
Fragments arranged by size:
1331 929 739 454 444
PvuII CAG'CTG
Cuts at: 0 14 1560 2967 3174 3897
Size: 14 1546 1407 207 723
Fragments arranged by size:
1546 1407 723 207 14
RsrII CG'GwC_CG
Cuts at: 0 733 3897
Size: 733 3164
SacI G_AGCT'C
Cuts at: 0 2017 3897
Size: 2017 1880
SacII CC_GC'GG
Cuts at: 0 3815 3897
Size: 3815 82
SalI G'TCGA_C
Cuts at: 0 2768 3897
Size: 2768 1129
SanDI GG'GwC_CC
Cuts at: 0 782 3897
Size: 782 3115
SapI GCTCTTCn'nnn_
Cuts at: 0 1078 3068 3897
Size: 1078 1990 829
Fragments arranged by size:
1990 1078 829
SfcI C'TryA_G
Cuts at: 0 1327 2256 2700 3154 3547 3897
Size: 1327 929 444 454 393 350
Fragments arranged by size:
1327 929 454 444 393 350
SfoI GGC'GCC
Cuts at: 0 227 3897
Size: 227 3670
SgrAI Cr'CCGG_yG
Cuts at: 0 228 958 3897
Size: 228 730 2939
Fragments arranged by size:
2939 730 228
SmaI CCC'GGG
Cuts at: 0 84 780 3897
Size: 84 696 3117
Fragments arranged by size:
3117 696 84
SmlI C'TyrA_G
Cuts at: 0 654 1725 3897
Size: 654 1071 2172
Fragments arranged by size:
2172 1071 654
SpeI A'CTAG_T
Cuts at: 0 313 3897
Size: 313 3584
SphI G_CATG'C
Cuts at: 0 2896 3523 3897
Size: 2896 627 374
Fragments arranged by size:
2896 627 374
SspI AAT'ATT
Cuts at: 0 3420 3897
Size: 3420 477
StuI AGG'CCT
Cuts at: 0 1306 1363 3688 3897
Size: 1306 57 2325 209
Fragments arranged by size:
2325 1306 209 57
StyI C'CwwG_G
Cuts at: 0 717 831 2432 3689 3897
Size: 717 114 1601 1257 208
Fragments arranged by size:
1601 1257 717 208 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 841 926 1041 1949 3897
Size: 841 85 115 908 1948
Fragments arranged by size:
1948 908 841 115 85
TatI w'GTAC_w
Cuts at: 0 614 1624 2057 2946 3131 3897
Size: 614 1010 433 889 185 766
Fragments arranged by size:
1010 889 766 614 433 185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1269 3897
Size: 1269 2628
XmaI C'CCGG_G
Cuts at: 0 82 778 3897
Size: 82 696 3119
Fragments arranged by size:
3119 696 82
XmnI GAAnn'nnTTC
Cuts at: 0 1493 2732 2756 3897
Size: 1493 1239 24 1141
Fragments arranged by size:
1493 1239 1141 24
Enzymes that do cut:
AccI AclI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtsI ClaI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PflMI PmlI PpuMI PshAI PsiI PstI
PvuII RsrII SacI SacII SalI SanDI SapI SfcI
SfoI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BfrBI BglI BmtI
BsaBI BsiEI BsiWI BstXI BstZ17I BtgZI CspCI DraIII
DrdI EagI EciI FseI FspI FspAI HpaI KpnI
MluI MscI NdeI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpiI PspOMI PspXI PsrI
PvuI SbfI ScaI SexAI SfiI SgfI SnaBI SrfI
SwaI TstI Tth111I XbaI XhoI ZraI