Restriction map for
pF1KA0609
(Linear) (Six-base) MAPSORT of: KIAA0609.seq Check: 2514 from: 1 to: 2112
KIAA0609.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AatII G_ACGT'C
Cuts at: 0 1265 2112
Size: 1265 847
Acc65I G'GTAC_C
Cuts at: 0 827 1721 2112
Size: 827 894 391
Fragments arranged by size:
894 827 391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 2112
Size: 638 1474
AfeI AGC'GCT
Cuts at: 0 1192 2112
Size: 1192 920
AflIII A'CryG_T
Cuts at: 0 1115 1517 2054 2112
Size: 1115 402 537 58
Fragments arranged by size:
1115 537 402 58
AleI CACnn'nnGTG
Cuts at: 0 892 2112
Size: 892 1220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1444 1476 1909 1941 2112
Size: 1444 32 433 32 171
Fragments arranged by size:
1444 433 171 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 543 1305 2112
Size: 543 762 807
Fragments arranged by size:
807 762 543
ApaI G_GGCC'C
Cuts at: 0 1337 2112
Size: 1337 775
ApoI r'AATT_y
Cuts at: 0 28 2112
Size: 28 2084
BamHI G'GATC_C
Cuts at: 0 629 2112
Size: 629 1483
BanI G'GyrC_C
Cuts at: 0 570 827 1721 2112
Size: 570 257 894 391
Fragments arranged by size:
894 570 391 257
BanII G_rGCy'C
Cuts at: 0 270 611 971 1337 1402 1559 2112
Size: 270 341 360 366 65 157 553
Fragments arranged by size:
553 366 360 341 270 157 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 43 2112
Size: 43 2069
BciVI GTATCCnnnnn_n'
Cuts at: 0 430 2112
Size: 430 1682
BglI GCCn_nnn'nGGC
Cuts at: 0 2069 2112
Size: 2069 43
BlpI GC'TnA_GC
Cuts at: 0 247 1277 2112
Size: 247 1030 835
Fragments arranged by size:
1030 835 247
Bme1580I G_kGCm'C
Cuts at: 0 322 575 1337 2112
Size: 322 253 762 775
Fragments arranged by size:
775 762 322 253
BmgBI CAC'GTC
Cuts at: 0 1116 1728 1837 2112
Size: 1116 612 109 275
Fragments arranged by size:
1116 612 275 109
BmrI ACTGGGnnnn_n'
Cuts at: 0 1335 2112
Size: 1335 777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 154 214 344 724 1117 1282 1327
Size: 153 1 60 130 380 393 165 45
Cuts at: 1327 2112
Size: 785
Fragments arranged by size:
785 393 380 165 153 130 60 45
1
Bpu10I CC'TnA_GC
Cuts at: 0 993 2112
Size: 993 1119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 2017 2112
Size: 595 1422 95
Fragments arranged by size:
1422 595 95
BsaHI Gr'CG_yC
Cuts at: 0 1262 1364 2112
Size: 1262 102 748
Fragments arranged by size:
1262 748 102
BsaWI w'CCGG_w
Cuts at: 0 1856 2112
Size: 1856 256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 151 181 928 958 1078 1108 1444 1474
Size: 151 30 747 30 120 30 336 30
Cuts at: 1474 1669 1699 1909 1939 2112
Size: 195 30 210 30 173
Fragments arranged by size:
747 336 210 195 173 151 120 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 235 328 2112
Size: 232 3 93 1784
Fragments arranged by size:
1784 232 93 3
BseYI C'CCAG_C
Cuts at: 0 3 63 1373 1972 2112
Size: 3 60 1310 599 140
Fragments arranged by size:
1310 599 140 60 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1093 1210 1933 2112
Size: 487 606 117 723 179
Fragments arranged by size:
723 606 487 179 117
BsiEI CG_ry'CG
Cuts at: 0 392 1857 2112
Size: 392 1465 255
Fragments arranged by size:
1465 392 255
BsiHKAI G_wGCw'C
Cuts at: 0 611 971 2112
Size: 611 360 1141
Fragments arranged by size:
1141 611 360
BsmBI CGTCTCn'nnnn_
Cuts at: 0 15 1106 1827 2112
Size: 15 1091 721 285
Fragments arranged by size:
1091 721 285 15
Bsp1286I G_dGCh'C
Cuts at: 0 270 322 575 611 971 1337 1402 1559
Size: 270 52 253 36 360 366 65 157
Cuts at: 1559 2112
Size: 553
Fragments arranged by size:
553 366 360 270 253 157 65 52
36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1484 2112
Size: 1484 628
BsrBI CCG'CTC
Cuts at: 0 1094 2112
Size: 1094 1018
BsrDI GCAATG_nn'
Cuts at: 0 527 749 1622 2112
Size: 527 222 873 490
Fragments arranged by size:
873 527 490 222
BsrFI r'CCGG_y
Cuts at: 0 387 1649 2112
Size: 387 1262 463
Fragments arranged by size:
1262 463 387
BsrGI T'GTAC_A
Cuts at: 0 1445 2112
Size: 1445 667
BstBI TT'CG_AA
Cuts at: 0 101 2112
Size: 101 2011
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1344 2112
Size: 1344 768
BstYI r'GATC_y
Cuts at: 0 539 629 2112
Size: 539 90 1483
Fragments arranged by size:
1483 539 90
Bsu36I CC'TnA_GG
Cuts at: 0 1570 2112
Size: 1570 542
BtgI C'CryG_G
Cuts at: 0 308 859 1286 2112
Size: 308 551 427 826
Fragments arranged by size:
826 551 427 308
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 262 2112
Size: 262 1850
BtsI GCAGTG_nn'
Cuts at: 0 1108 1639 2112
Size: 1108 531 473
Fragments arranged by size:
1108 531 473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 732 767 2112
Size: 732 35 1345
Fragments arranged by size:
1345 732 35
DraIII CAC_nnn'GTG
Cuts at: 0 1428 1443 2112
Size: 1428 15 669
Fragments arranged by size:
1428 669 15
EaeI y'GGCC_r
Cuts at: 0 545 862 1490 2112
Size: 545 317 628 622
Fragments arranged by size:
628 622 545 317
EarI CTCTTCn'nnn_
Cuts at: 0 559 695 870 1714 2033 2112
Size: 559 136 175 844 319 79
Fragments arranged by size:
844 559 319 175 136 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1785 2112
Size: 1785 327
EcoICRI GAG'CTC
Cuts at: 0 609 969 2112
Size: 609 360 1143
Fragments arranged by size:
1143 609 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 154 214 344 638 724 1117 1282
Size: 153 1 60 130 294 86 393 165
Cuts at: 1282 1327 2112
Size: 45 785
Fragments arranged by size:
785 393 294 165 153 130 86 60
45 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 2112
Size: 256 1856
EcoO109I rG'GnC_Cy
Cuts at: 0 1333 1703 2112
Size: 1333 370 409
Fragments arranged by size:
1333 409 370
EcoRV GAT'ATC
Cuts at: 0 732 2112
Size: 732 1380
FspI TGC'GCA
Cuts at: 0 202 1483 2112
Size: 202 1281 629
Fragments arranged by size:
1281 629 202
FspAI rTGC'GCAy
Cuts at: 0 202 2112
Size: 202 1910
HaeII r_GCGC'y
Cuts at: 0 1194 2112
Size: 1194 918
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 645 672 677 701 704 733
Size: 598 32 15 27 5 24 3 29
Cuts at: 733 1669 1701 2112
Size: 936 32 411
Fragments arranged by size:
936 598 411 32 32 29 27 24
15 5 3
HindIII A'AGCT_T
Cuts at: 0 670 2112
Size: 670 1442
KpnI G_GTAC'C
Cuts at: 0 831 1725 2112
Size: 831 894 387
Fragments arranged by size:
894 831 387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2024 2112
Size: 2024 88
MscI TGG'CCA
Cuts at: 0 547 864 1492 2112
Size: 547 317 628 620
Fragments arranged by size:
628 620 547 317
MslI CAynn'nnrTG
Cuts at: 0 892 976 2112
Size: 892 84 1136
Fragments arranged by size:
1136 892 84
MspA1I CmG'CkG
Cuts at: 0 132 177 577 1077 1143 1281 2112
Size: 132 45 400 500 66 138 831
Fragments arranged by size:
831 500 400 138 132 66 45
NcoI C'CATG_G
Cuts at: 0 308 859 1286 2112
Size: 308 551 427 826
Fragments arranged by size:
826 551 427 308
NspI r_CATG'y
Cuts at: 0 207 981 1521 1962 2058 2112
Size: 207 774 540 441 96 54
Fragments arranged by size:
774 540 441 207 96 54
PasI CC'CwG_GG
Cuts at: 0 258 2112
Size: 258 1854
PciI A'CATG_T
Cuts at: 0 1517 2054 2112
Size: 1517 537 58
Fragments arranged by size:
1517 537 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 74 871 1305 1428 2112
Size: 74 797 434 123 684
Fragments arranged by size:
797 684 434 123 74
PfoI T'CCnGG_A
Cuts at: 0 625 2112
Size: 625 1487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1444 1476 1909 1941 2112
Size: 1444 32 433 32 171
Fragments arranged by size:
1444 433 171 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1703 2112
Size: 1703 409
PspOMI G'GGCC_C
Cuts at: 0 1333 2112
Size: 1333 779
PstI C_TGCA'G
Cuts at: 0 16 1248 2112
Size: 16 1232 864
Fragments arranged by size:
1232 864 16
PvuII CAG'CTG
Cuts at: 0 1077 1143 1281 2112
Size: 1077 66 138 831
Fragments arranged by size:
1077 831 138 66
RsrII CG'GwC_CG
Cuts at: 0 1772 2112
Size: 1772 340
SacI G_AGCT'C
Cuts at: 0 611 971 2112
Size: 611 360 1141
Fragments arranged by size:
1141 611 360
SanDI GG'GwC_CC
Cuts at: 0 1703 2112
Size: 1703 409
SapI GCTCTTCn'nnn_
Cuts at: 0 559 2112
Size: 559 1553
SfcI C'TryA_G
Cuts at: 0 12 1107 1244 2112
Size: 12 1095 137 868
Fragments arranged by size:
1095 868 137 12
SmlI C'TyrA_G
Cuts at: 0 610 2032 2112
Size: 610 1422 80
Fragments arranged by size:
1422 610 80
SphI G_CATG'C
Cuts at: 0 207 981 2112
Size: 207 774 1131
Fragments arranged by size:
1131 774 207
StyI C'CwwG_G
Cuts at: 0 308 831 859 870 1286 2112
Size: 308 523 28 11 416 826
Fragments arranged by size:
826 523 416 308 28 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1225 1764 2112
Size: 1225 539 348
Fragments arranged by size:
1225 539 348
TatI w'GTAC_w
Cuts at: 0 1217 1445 2112
Size: 1217 228 667
Fragments arranged by size:
1217 667 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 101 133 928 960 1078 1110 1153 1185
Size: 101 32 795 32 118 32 43 32
Cuts at: 1185 2112
Size: 927
Fragments arranged by size:
927 795 118 101 43 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 71 2112
Size: 71 2041
XmnI GAAnn'nnTTC
Cuts at: 0 2042 2112
Size: 2042 70
ZraI GAC'GTC
Cuts at: 0 1263 2112
Size: 1263 849
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflIII AleI AloI AlwNI
ApaI ApoI BamHI BanI BanII BbsI BciVI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI
FspAI HaeII Hin4I HindIII KpnI MmeI MscI MslI
MspA1I NcoI NspI PasI PciI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII RsrII SacI SanDI SapI
SfcI SmlI SphI StyI TaqII TatI TstI XcmI
XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AgeI AhdI AlfI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BcgI BclI BfrBI BglII BmtI BplI BsaI BsaAI
BsaBI BsiWI BsmI BspEI BspHI BssHII BssSI BstAPI
BstEII BstZ17I ClaI DraI DrdI EagI EcoRI FalI
FseI HincII HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI