Restriction map for
pF1KA0610
(Linear) (Six-base) MAPSORT of: KIAA0610.seq Check: 3094 from: 1 to: 1998
KIAA0610.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 164 474 1998
Size: 164 310 1524
Fragments arranged by size:
1524 310 164
Acc65I G'GTAC_C
Cuts at: 0 737 1998
Size: 737 1261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 437 557 790 1998
Size: 304 133 120 233 1208
Fragments arranged by size:
1208 304 233 133 120
AflIII A'CryG_T
Cuts at: 0 362 878 1533 1998
Size: 362 516 655 465
Fragments arranged by size:
655 516 465 362
AleI CACnn'nnGTG
Cuts at: 0 1102 1998
Size: 1102 896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 927 1698 1998
Size: 927 771 300
Fragments arranged by size:
927 771 300
AlwNI CAG_nnn'CTG
Cuts at: 0 205 460 571 1479 1711 1998
Size: 205 255 111 908 232 287
Fragments arranged by size:
908 287 255 232 205 111
ApaLI G'TGCA_C
Cuts at: 0 719 1998
Size: 719 1279
ApoI r'AATT_y
Cuts at: 0 278 346 1039 1998
Size: 278 68 693 959
Fragments arranged by size:
959 693 278 68
AvaI C'yCGr_G
Cuts at: 0 796 1998
Size: 796 1202
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 712 745 1806 1839 1998
Size: 712 33 1061 33 159
Fragments arranged by size:
1061 712 159 33 33
BanI G'GyrC_C
Cuts at: 0 327 737 1998
Size: 327 410 1261
Fragments arranged by size:
1261 410 327
BanII G_rGCy'C
Cuts at: 0 965 1998
Size: 965 1033
BbvCI CC'TCA_GC
Cuts at: 0 407 1998
Size: 407 1591
BciVI GTATCCnnnnn_n'
Cuts at: 0 564 740 1998
Size: 564 176 1258
Fragments arranged by size:
1258 564 176
BfrBI ATG'CAT
Cuts at: 0 1685 1998
Size: 1685 313
BglII A'GATC_T
Cuts at: 0 844 1998
Size: 844 1154
Bme1580I G_kGCm'C
Cuts at: 0 332 723 1998
Size: 332 391 1275
Fragments arranged by size:
1275 391 332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1998
Size: 988 1010
Bpu10I CC'TnA_GC
Cuts at: 0 407 1998
Size: 407 1591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 652 1894 1998
Size: 512 140 1242 104
Fragments arranged by size:
1242 512 140 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 628 1998
Size: 628 1370
BsaWI w'CCGG_w
Cuts at: 0 849 1998
Size: 849 1149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 423 453 1853 1883 1998
Size: 423 30 1400 30 115
Fragments arranged by size:
1400 423 115 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 395 500 515 610 1059 1469 1589 1998
Size: 395 105 15 95 449 410 120 409
Fragments arranged by size:
449 410 409 395 120 105 95 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 467 1694 1998
Size: 446 21 1227 304
Fragments arranged by size:
1227 446 304 21
BsiHKAI G_wGCw'C
Cuts at: 0 723 965 1998
Size: 723 242 1033
Fragments arranged by size:
1033 723 242
Bsp1286I G_dGCh'C
Cuts at: 0 332 723 965 1998
Size: 332 391 242 1033
Fragments arranged by size:
1033 391 332 242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 36 164 474 1998
Size: 36 128 310 1524
Fragments arranged by size:
1524 310 128 36
BsrBI CCG'CTC
Cuts at: 0 1374 1998
Size: 1374 624
BsrDI GCAATG_nn'
Cuts at: 0 1838 1998
Size: 1838 160
BsrGI T'GTAC_A
Cuts at: 0 1713 1998
Size: 1713 285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 460 1998
Size: 460 1538
BstBI TT'CG_AA
Cuts at: 0 744 1998
Size: 744 1254
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1051 1998
Size: 1051 947
BstYI r'GATC_y
Cuts at: 0 844 972 1998
Size: 844 128 1026
Fragments arranged by size:
1026 844 128
Bsu36I CC'TnA_GG
Cuts at: 0 639 1998
Size: 639 1359
BtgI C'CryG_G
Cuts at: 0 1397 1998
Size: 1397 601
EcoICRI GAG'CTC
Cuts at: 0 963 1998
Size: 963 1035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 437 557 790 988 1998
Size: 304 133 120 233 198 1010
Fragments arranged by size:
1010 304 233 198 133 120
EcoO109I rG'GnC_Cy
Cuts at: 0 202 1998
Size: 202 1796
EcoRI G'AATT_C
Cuts at: 0 1039 1998
Size: 1039 959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 84 1998
Size: 84 1914
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 373 405 568 600 1579 1611
Size: 143 32 198 32 163 32 979 32
Cuts at: 1611 1853 1885 1896 1928 1998
Size: 242 32 11 32 70
Fragments arranged by size:
979 242 198 163 143 70 32 32
32 32 32 32 11
HincII GTy'rAC
Cuts at: 0 81 1191 1692 1935 1998
Size: 81 1110 501 243 63
Fragments arranged by size:
1110 501 243 81 63
HpaI GTT'AAC
Cuts at: 0 81 1191 1692 1998
Size: 81 1110 501 306
Fragments arranged by size:
1110 501 306 81
KpnI G_GTAC'C
Cuts at: 0 741 1998
Size: 741 1257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 1094 1494 1998
Size: 570 524 400 504
Fragments arranged by size:
570 524 504 400
MslI CAynn'nnrTG
Cuts at: 0 1102 1998
Size: 1102 896
MspA1I CmG'CkG
Cuts at: 0 1414 1998
Size: 1414 584
NsiI A_TGCA'T
Cuts at: 0 1687 1998
Size: 1687 311
NspI r_CATG'y
Cuts at: 0 366 415 882 1537 1998
Size: 366 49 467 655 461
Fragments arranged by size:
655 467 461 366 49
PciI A'CATG_T
Cuts at: 0 362 878 1533 1998
Size: 362 516 655 465
Fragments arranged by size:
655 516 465 362
PflMI CCAn_nnn'nTGG
Cuts at: 0 19 1770 1998
Size: 19 1751 228
Fragments arranged by size:
1751 228 19
PpuMI rG'GwC_Cy
Cuts at: 0 202 1998
Size: 202 1796
PstI C_TGCA'G
Cuts at: 0 311 708 1998
Size: 311 397 1290
Fragments arranged by size:
1290 397 311
PvuII CAG'CTG
Cuts at: 0 1414 1998
Size: 1414 584
SacI G_AGCT'C
Cuts at: 0 965 1998
Size: 965 1033
SbfI CC_TGCA'GG
Cuts at: 0 708 1998
Size: 708 1290
SfcI C'TryA_G
Cuts at: 0 253 307 704 1448 1499 1998
Size: 253 54 397 744 51 499
Fragments arranged by size:
744 499 397 253 54 51
SmaI CCC'GGG
Cuts at: 0 798 1998
Size: 798 1200
SmlI C'TyrA_G
Cuts at: 0 527 631 1873 1998
Size: 527 104 1242 125
Fragments arranged by size:
1242 527 125 104
SphI G_CATG'C
Cuts at: 0 415 1998
Size: 415 1583
SspI AAT'ATT
Cuts at: 0 1809 1998
Size: 1809 189
StuI AGG'CCT
Cuts at: 0 67 1998
Size: 67 1931
StyI C'CwwG_G
Cuts at: 0 1119 1421 1998
Size: 1119 302 577
Fragments arranged by size:
1119 577 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 197 1287 1998
Size: 197 1090 711
Fragments arranged by size:
1090 711 197
TatI w'GTAC_w
Cuts at: 0 681 862 1713 1998
Size: 681 181 851 285
Fragments arranged by size:
851 681 285 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 423 455 902 934 1998
Size: 423 32 447 32 1064
Fragments arranged by size:
1064 447 423 32 32
XbaI T'CTAG_A
Cuts at: 0 282 1998
Size: 282 1716
XmaI C'CCGG_G
Cuts at: 0 796 1998
Size: 796 1202
XmnI GAAnn'nnTTC
Cuts at: 0 567 1998
Size: 567 1431
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI AvaI BaeI BanI BanII BbvCI BciVI BfrBI
BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI
BstAPI BstBI BstXI BstYI Bsu36I BtgI EcoICRI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HpaI KpnI MmeI
MslI MspA1I NsiI NspI PciI PflMI PpuMI PstI
PvuII SacI SbfI SfcI SmaI SmlI SphI SspI
StuI StyI TaqII TatI TstI XbaI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BamHI BbeI BbsI
BcgI BclI BglI BlpI BmgBI BmrI BmtI BplI
BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BssHII BssSI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoNI EcoRV FseI FspI FspAI HaeII
HindIII KasI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI
Tth111I XcmI XhoI ZraI