Restriction map for
pF1KSDA0613
(Linear) (Six-base) MAPSORT of: KIAA0613.seq Check: 9661 from: 1 to: 2181
KIAA0613.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 978 1140 1401 1903 2086 2181
Size: 978 162 261 502 183 95
Fragments arranged by size:
978 502 261 183 162 95
AccI GT'mk_AC
Cuts at: 0 716 734 1220 2181
Size: 716 18 486 961
Fragments arranged by size:
961 716 486 18
AclI AA'CG_TT
Cuts at: 0 1784 2181
Size: 1784 397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1109 1736 2181
Size: 1109 627 445
Fragments arranged by size:
1109 627 445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1776 1832 2181
Size: 1776 56 349
Fragments arranged by size:
1776 349 56
AlwNI CAG_nnn'CTG
Cuts at: 0 1018 1057 1132 1234 1539 2181
Size: 1018 39 75 102 305 642
Fragments arranged by size:
1018 642 305 102 75 39
ApaI G_GGCC'C
Cuts at: 0 29 35 531 1162 1441 1681 2181
Size: 29 6 496 631 279 240 500
Fragments arranged by size:
631 500 496 279 240 29 6
ApaLI G'TGCA_C
Cuts at: 0 921 2181
Size: 921 1260
ApoI r'AATT_y
Cuts at: 0 853 2181
Size: 853 1328
AvaI C'yCGr_G
Cuts at: 0 28 530 564 666 1184 1503 1544 2181
Size: 28 502 34 102 518 319 41 637
Fragments arranged by size:
637 518 502 319 102 41 34 28
BanI G'GyrC_C
Cuts at: 0 354 391 400 1581 1709 2181
Size: 354 37 9 1181 128 472
Fragments arranged by size:
1181 472 354 128 37 9
BanII G_rGCy'C
Cuts at: 0 29 35 413 531 565 1162 1441 1681
Size: 29 6 378 118 34 597 279 240
Cuts at: 1681 1964 2181
Size: 283 217
Fragments arranged by size:
597 378 283 279 240 217 118 34
29 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1958 2181
Size: 1958 223
BfrBI ATG'CAT
Cuts at: 0 1866 2181
Size: 1866 315
BlpI GC'TnA_GC
Cuts at: 0 127 1616 2181
Size: 127 1489 565
Fragments arranged by size:
1489 565 127
Bme1580I G_kGCm'C
Cuts at: 0 29 35 531 792 925 1162 1441 1681
Size: 29 6 496 261 133 237 279 240
Cuts at: 1681 2181
Size: 500
Fragments arranged by size:
500 496 279 261 240 237 133 29
6
BmrI ACTGGGnnnn_n'
Cuts at: 0 32 111 980 2181
Size: 32 79 869 1201
Fragments arranged by size:
1201 869 79 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 709 2181
Size: 709 1472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 2131 2181
Size: 266 1865 50
Fragments arranged by size:
1865 266 50
Bpu10I CC'TnA_GC
Cuts at: 0 412 2181
Size: 412 1769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 1893 2181
Size: 246 1647 288
Fragments arranged by size:
1647 288 246
BsaI GGTCTCn'nnnn_
Cuts at: 0 607 2181
Size: 607 1574
BsaHI Gr'CG_yC
Cuts at: 0 161 2181
Size: 161 2020
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 264 294 1276 1300 1306 1330 1344 1374
Size: 264 30 982 24 6 24 14 30
Cuts at: 1374 2181
Size: 807
Fragments arranged by size:
982 807 264 30 30 24 24 14
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 447 2181
Size: 447 1734
BseYI C'CCAG_C
Cuts at: 0 122 358 364 962 1368 1554 1566 1626
Size: 122 236 6 598 406 186 12 60
Cuts at: 1626 2181
Size: 555
Fragments arranged by size:
598 555 406 236 186 122 60 12
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 1397 2181
Size: 1157 240 784
Fragments arranged by size:
1157 784 240
BsiHKAI G_wGCw'C
Cuts at: 0 413 925 1516 2181
Size: 413 512 591 665
Fragments arranged by size:
665 591 512 413
Bsp1286I G_dGCh'C
Cuts at: 0 29 35 413 531 565 792 925 1162
Size: 29 6 378 118 34 227 133 237
Cuts at: 1162 1441 1516 1681 1964 2181
Size: 279 75 165 283 217
Fragments arranged by size:
378 283 279 237 227 217 165 133
118 75 34 29 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 815 978 1140 1401 1903 2086 2181
Size: 815 163 162 261 502 183 95
Fragments arranged by size:
815 502 261 183 163 162 95
BsrBI CCG'CTC
Cuts at: 0 328 2181
Size: 328 1853
BsrFI r'CCGG_y
Cuts at: 0 300 915 2181
Size: 300 615 1266
Fragments arranged by size:
1266 615 300
BssSI C'ACGA_G
Cuts at: 0 142 2181
Size: 142 2039
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1539 2003 2181
Size: 1539 464 178
Fragments arranged by size:
1539 464 178
BstEII G'GTnAC_C
Cuts at: 0 136 2181
Size: 136 2045
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1851 2181
Size: 1851 330
Bsu36I CC'TnA_GG
Cuts at: 0 371 502 619 1577 2181
Size: 371 131 117 958 604
Fragments arranged by size:
958 604 371 131 117
BtgI C'CryG_G
Cuts at: 0 490 1694 2007 2024 2181
Size: 490 1204 313 17 157
Fragments arranged by size:
1204 490 313 157 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1017 1812 2181
Size: 1017 795 369
Fragments arranged by size:
1017 795 369
BtsI GCAGTG_nn'
Cuts at: 0 1025 1118 1196 1656 2181
Size: 1025 93 78 460 525
Fragments arranged by size:
1025 525 460 93 78
DraIII CAC_nnn'GTG
Cuts at: 0 2003 2181
Size: 2003 178
EaeI y'GGCC_r
Cuts at: 0 149 1007 1115 2181
Size: 149 858 108 1066
Fragments arranged by size:
1066 858 149 108
EarI CTCTTCn'nnn_
Cuts at: 0 1101 1128 1712 1766 1945 2181
Size: 1101 27 584 54 179 236
Fragments arranged by size:
1101 584 236 179 54 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 438 1029 2181
Size: 438 591 1152
Fragments arranged by size:
1152 591 438
EcoICRI GAG'CTC
Cuts at: 0 411 2181
Size: 411 1770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1109 1736 2131 2181
Size: 266 843 627 395 50
Fragments arranged by size:
843 627 395 266 50
EcoO109I rG'GnC_Cy
Cuts at: 0 32 321 527 534 705 1085 1158 1159
Size: 32 289 206 7 171 380 73 1
Cuts at: 1159 1437 1438 1573 1677 2181
Size: 278 1 135 104 504
Fragments arranged by size:
504 380 289 278 206 171 135 104
73 32 7 1 1
EcoRI G'AATT_C
Cuts at: 0 853 2181
Size: 853 1328
FspI TGC'GCA
Cuts at: 0 2091 2181
Size: 2091 90
HaeII r_GCGC'y
Cuts at: 0 725 2181
Size: 725 1456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 264 296 709 741 1471 1503 2181
Size: 264 32 413 32 730 32 678
Fragments arranged by size:
730 678 413 264 32 32 32
HincII GTy'rAC
Cuts at: 0 165 2181
Size: 165 2016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 827 2181
Size: 654 173 1354
Fragments arranged by size:
1354 654 173
MscI TGG'CCA
Cuts at: 0 151 1009 2181
Size: 151 858 1172
Fragments arranged by size:
1172 858 151
MspA1I CmG'CkG
Cuts at: 0 492 945 1015 1433 1570 1824 2181
Size: 492 453 70 418 137 254 357
Fragments arranged by size:
492 453 418 357 254 137 70
NcoI C'CATG_G
Cuts at: 0 1694 2007 2181
Size: 1694 313 174
Fragments arranged by size:
1694 313 174
NsiI A_TGCA'T
Cuts at: 0 1868 2181
Size: 1868 313
NspI r_CATG'y
Cuts at: 0 75 913 1870 2181
Size: 75 838 957 311
Fragments arranged by size:
957 838 311 75
PasI CC'CwG_GG
Cuts at: 0 35 1258 1466 2055 2181
Size: 35 1223 208 589 126
Fragments arranged by size:
1223 589 208 126 35
PfoI T'CCnGG_A
Cuts at: 0 88 2181
Size: 88 2093
PpuMI rG'GwC_Cy
Cuts at: 0 321 534 705 1573 2181
Size: 321 213 171 868 608
Fragments arranged by size:
868 608 321 213 171
PspOMI G'GGCC_C
Cuts at: 0 25 31 527 1158 1437 1677 2181
Size: 25 6 496 631 279 240 504
Fragments arranged by size:
631 504 496 279 240 25 6
PspXI vC'TCGA_Gb
Cuts at: 0 564 2181
Size: 564 1617
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1771 1803 2181
Size: 1771 32 378
Fragments arranged by size:
1771 378 32
PstI C_TGCA'G
Cuts at: 0 53 242 812 2181
Size: 53 189 570 1369
Fragments arranged by size:
1369 570 189 53
PvuII CAG'CTG
Cuts at: 0 1015 2181
Size: 1015 1166
SacI G_AGCT'C
Cuts at: 0 413 2181
Size: 413 1768
SacII CC_GC'GG
Cuts at: 0 493 2181
Size: 493 1688
SapI GCTCTTCn'nnn_
Cuts at: 0 1766 2181
Size: 1766 415
SfcI C'TryA_G
Cuts at: 0 49 238 808 1101 1149 1245 1428 2181
Size: 49 189 570 293 48 96 183 753
Fragments arranged by size:
753 570 293 189 183 96 49 48
SgrAI Cr'CCGG_yG
Cuts at: 0 915 2181
Size: 915 1266
SmaI CCC'GGG
Cuts at: 0 30 532 1186 1505 1546 2181
Size: 30 502 654 319 41 635
Fragments arranged by size:
654 635 502 319 41 30
SmlI C'TyrA_G
Cuts at: 0 225 564 1872 2181
Size: 225 339 1308 309
Fragments arranged by size:
1308 339 309 225
SphI G_CATG'C
Cuts at: 0 913 1870 2181
Size: 913 957 311
Fragments arranged by size:
957 913 311
SrfI GCCC'GGGC
Cuts at: 0 30 2181
Size: 30 2151
StuI AGG'CCT
Cuts at: 0 472 664 1093 2181
Size: 472 192 429 1088
Fragments arranged by size:
1088 472 429 192
StyI C'CwwG_G
Cuts at: 0 1081 1694 2007 2181
Size: 1081 613 313 174
Fragments arranged by size:
1081 613 313 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 372 1153 1191 1458 2181
Size: 372 781 38 267 723
Fragments arranged by size:
781 723 372 267 38
TatI w'GTAC_w
Cuts at: 0 602 2181
Size: 602 1579
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 1344 1376 2181
Size: 269 32 1043 32 805
Fragments arranged by size:
1043 805 269 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 160 2181
Size: 160 2021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 183 2181
Size: 183 1998
XhoI C'TCGA_G
Cuts at: 0 564 2181
Size: 564 1617
XmaI C'CCGG_G
Cuts at: 0 28 530 1184 1503 1544 2181
Size: 28 502 654 319 41 637
Fragments arranged by size:
654 637 502 319 41 28
Enzymes that do cut:
AarI AccI AclI AcuI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbsI BfrBI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstEII BstXI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII MmeI MscI MspA1I NcoI
NsiI NspI PasI PfoI PpuMI PspOMI PspXI PsrI
PstI PvuII SacI SacII SapI SfcI SgrAI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BglI BglII BmgBI BmtI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstYI BstZ17I ClaI
CspCI DraI DrdI EagI EcoNI EcoRV FalI FseI
FspAI HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SnaBI SpeI SspI SwaI XbaI XmnI ZraI