Restriction map for
pF1KA0614
(Linear) (Six-base) MAPSORT of: KIAA0614.seq Check: 9731 from: 1 to: 9000
KIAA0614.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1065 4460 5190 7971 8614 8791 9000
Size: 1065 3395 730 2781 643 177 209
Fragments arranged by size:
3395 2781 1065 730 643 209 177
AatII G_ACGT'C
Cuts at: 0 3335 8156 9000
Size: 3335 4821 844
Fragments arranged by size:
4821 3335 844
AccI GT'mk_AC
Cuts at: 0 7639 9000
Size: 7639 1361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 2164 7684 9000
Size: 673 1491 5520 1316
Fragments arranged by size:
5520 1491 1316 673
AfeI AGC'GCT
Cuts at: 0 2922 3483 9000
Size: 2922 561 5517
Fragments arranged by size:
5517 2922 561
AflII C'TTAA_G
Cuts at: 0 7119 9000
Size: 7119 1881
AflIII A'CryG_T
Cuts at: 0 718 4226 4943 9000
Size: 718 3508 717 4057
Fragments arranged by size:
4057 3508 718 717
AleI CACnn'nnGTG
Cuts at: 0 7699 8978 9000
Size: 7699 1279 22
Fragments arranged by size:
7699 1279 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 521 4381 9000
Size: 521 3860 4619
Fragments arranged by size:
4619 3860 521
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2243 2275 9000
Size: 2243 32 6725
Fragments arranged by size:
6725 2243 32
AlwNI CAG_nnn'CTG
Cuts at: 0 274 447 606 1051 1864 2557 2674 5124
Size: 274 173 159 445 813 693 117 2450
Cuts at: 5124 5868 5910 6807 6960 8109 8130 8334 8568
Size: 744 42 897 153 1149 21 204 234
Cuts at: 8568 9000
Size: 432
Fragments arranged by size:
2450 1149 897 813 744 693 445 432
274 234 204 173 159 153 117 42
21
ApaI G_GGCC'C
Cuts at: 0 5920 8709 9000
Size: 5920 2789 291
Fragments arranged by size:
5920 2789 291
ApaLI G'TGCA_C
Cuts at: 0 610 1054 2216 3145 3456 5299 9000
Size: 610 444 1162 929 311 1843 3701
Fragments arranged by size:
3701 1843 1162 929 610 444 311
ApoI r'AATT_y
Cuts at: 0 105 373 1539 2279 2650 3663 3907 4087
Size: 105 268 1166 740 371 1013 244 180
Cuts at: 4087 4304 4767 6551 7255 9000
Size: 217 463 1784 704 1745
Fragments arranged by size:
1784 1745 1166 1013 740 704 463 371
268 244 217 180 105
AvaI C'yCGr_G
Cuts at: 0 1106 2939 3386 4774 5788 6115 6292 6533
Size: 1106 1833 447 1388 1014 327 177 241
Cuts at: 6533 8076 8629 9000
Size: 1543 553 371
Fragments arranged by size:
1833 1543 1388 1106 1014 553 447 371
327 241 177
BamHI G'GATC_C
Cuts at: 0 1401 2251 5954 6273 9000
Size: 1401 850 3703 319 2727
Fragments arranged by size:
3703 2727 1401 850 319
BanI G'GyrC_C
Cuts at: 0 517 1696 2034 3538 4498 4848 5321 5446
Size: 517 1179 338 1504 960 350 473 125
Cuts at: 5446 5525 5989 6744 7948 9000
Size: 79 464 755 1204 1052
Fragments arranged by size:
1504 1204 1179 1052 960 755 517 473
464 350 338 125 79
BanII G_rGCy'C
Cuts at: 0 1355 2842 3653 5288 5402 5461 5920 6116
Size: 1355 1487 811 1635 114 59 459 196
Cuts at: 6116 6326 6410 6451 6694 8083 8210 8350 8548
Size: 210 84 41 243 1389 127 140 198
Cuts at: 8548 8709 9000
Size: 161 291
Fragments arranged by size:
1635 1487 1389 1355 811 459 291 243
210 198 196 161 140 127 114 84
59 41
BbeI G_GCGC'C
Cuts at: 0 1700 5450 5993 9000
Size: 1700 3750 543 3007
Fragments arranged by size:
3750 3007 1700 543
BbsI GAAGACnn'nnnn_
Cuts at: 0 1162 1340 1628 2636 2820 3335 3816 3971
Size: 1162 178 288 1008 184 515 481 155
Cuts at: 3971 5726 6018 6068 6127 8197 8990 9000
Size: 1755 292 50 59 2070 793 10
Fragments arranged by size:
2070 1755 1162 1008 793 515 481 292
288 184 178 155 59 50 10
BbvCI CC'TCA_GC
Cuts at: 0 2755 5589 9000
Size: 2755 2834 3411
Fragments arranged by size:
3411 2834 2755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 194 228 9000
Size: 194 34 8772
Fragments arranged by size:
8772 194 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4226 9000
Size: 4226 4774
BclI T'GATC_A
Cuts at: 0 8912 9000
Size: 8912 88
BfrBI ATG'CAT
Cuts at: 0 7349 9000
Size: 7349 1651
BglI GCCn_nnn'nGGC
Cuts at: 0 976 5905 8533 9000
Size: 976 4929 2628 467
Fragments arranged by size:
4929 2628 976 467
BglII A'GATC_T
Cuts at: 0 4682 6038 9000
Size: 4682 1356 2962
Fragments arranged by size:
4682 2962 1356
BlpI GC'TnA_GC
Cuts at: 0 3605 3652 5767 6808 8037 8578 9000
Size: 3605 47 2115 1041 1229 541 422
Fragments arranged by size:
3605 2115 1229 1041 541 422 47
Bme1580I G_kGCm'C
Cuts at: 0 614 1058 1809 2220 2947 3115 3149 3460
Size: 614 444 751 411 727 168 34 311
Cuts at: 3460 4860 5303 5920 6749 6792 7538 8032 8709
Size: 1400 443 617 829 43 746 494 677
Cuts at: 8709 8825 9000
Size: 116 175
Fragments arranged by size:
1400 829 751 746 727 677 617 614
494 444 443 411 311 175 168 116
43 34
BmgBI CAC'GTC
Cuts at: 0 3195 3216 4764 6164 7404 9000
Size: 3195 21 1548 1400 1240 1596
Fragments arranged by size:
3195 1596 1548 1400 1240 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 204 3006 4970 5044 5442 8919 9000
Size: 204 2802 1964 74 398 3477 81
Fragments arranged by size:
3477 2802 1964 398 204 81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 951 1947 4633 6447 8340 9000
Size: 951 996 2686 1814 1893 660
Fragments arranged by size:
2686 1893 1814 996 951 660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1138 1279 1383 1586 1630 2735 3144
Size: 921 217 141 104 203 44 1105 409
Cuts at: 3144 4022 4465 4494 5075 5107 5329 5338 6958
Size: 878 443 29 581 32 222 9 1620
Cuts at: 6958 7317 7414 7612 8320 8608 8731 8971 9000
Size: 359 97 198 708 288 123 240 29
Fragments arranged by size:
1620 1105 921 878 708 581 443 409
359 288 240 222 217 203 198 141
123 104 97 44 32 29 29 9
Bpu10I CC'TnA_GC
Cuts at: 0 1213 2755 5589 8219 9000
Size: 1213 1542 2834 2630 781
Fragments arranged by size:
2834 2630 1542 1213 781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1170 6668 7362 8623 9000
Size: 933 237 5498 694 1261 377
Fragments arranged by size:
5498 1261 933 694 377 237
BsaI GGTCTCn'nnnn_
Cuts at: 0 754 3778 3935 4021 5442 7588 8284 8890
Size: 754 3024 157 86 1421 2146 696 606
Cuts at: 8890 9000
Size: 110
Fragments arranged by size:
3024 2146 1421 754 696 606 157 110
86
BsaAI yAC'GTr
Cuts at: 0 405 5501 9000
Size: 405 5096 3499
Fragments arranged by size:
5096 3499 405
BsaBI GATnn'nnATC
Cuts at: 0 1559 1606 6043 9000
Size: 1559 47 4437 2957
Fragments arranged by size:
4437 2957 1559 47
BsaHI Gr'CG_yC
Cuts at: 0 396 1697 3332 5447 5885 5990 8153 9000
Size: 396 1301 1635 2115 438 105 2163 847
Fragments arranged by size:
2163 2115 1635 1301 847 438 396 105
BsaWI w'CCGG_w
Cuts at: 0 3040 8480 9000
Size: 3040 5440 520
Fragments arranged by size:
5440 3040 520
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2728 2758 3240 3270 3385 3415 4020 4050
Size: 2728 30 482 30 115 30 605 30
Cuts at: 4050 4472 4502 5605 5635 6433 6463 8293 8323
Size: 422 30 1103 30 798 30 1830 30
Cuts at: 8323 8719 8749 9000
Size: 396 30 251
Fragments arranged by size:
2728 1830 1103 798 605 482 422 396
251 115 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1231 3248 4465 4475 4669 4824 5331 5434
Size: 1231 2017 1217 10 194 155 507 103
Cuts at: 5434 5613 5616 5619 5659 5703 6883 7951 8113
Size: 179 3 3 40 44 1180 1068 162
Cuts at: 8113 8419 8743 8922 9000
Size: 306 324 179 78
Fragments arranged by size:
2017 1231 1217 1180 1068 507 324 306
194 179 179 162 155 103 78 44
40 10 3 3
BseYI C'CCAG_C
Cuts at: 0 1138 1405 1413 2618 2917 2923 3230 4242
Size: 1138 267 8 1205 299 6 307 1012
Cuts at: 4242 4950 5662 5868 8032 8130 8327 9000
Size: 708 712 206 2164 98 197 673
Fragments arranged by size:
2164 1205 1138 1012 712 708 673 307
299 267 206 197 98 8 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 907 4186 4627 6387 7435 7756 8095
Size: 548 359 3279 441 1760 1048 321 339
Cuts at: 8095 8439 9000
Size: 344 561
Fragments arranged by size:
3279 1760 1048 561 548 441 359 344
339 321
BsiEI CG_ry'CG
Cuts at: 0 3138 3409 5477 5597 6922 8528 9000
Size: 3138 271 2068 120 1325 1606 472
Fragments arranged by size:
3138 2068 1606 1325 472 271 120
BsiHKAI G_wGCw'C
Cuts at: 0 614 792 1058 1355 2097 2220 2781 3149
Size: 614 178 266 297 742 123 561 368
Cuts at: 3149 3460 3653 4451 4868 5288 5303 5402 5768
Size: 311 193 798 417 420 15 99 366
Cuts at: 5768 7051 7097 9000
Size: 1283 46 1903
Fragments arranged by size:
1903 1283 798 742 614 561 420 417
368 366 311 297 266 193 178 123
99 46 15
BsiWI C'GTAC_G
Cuts at: 0 639 9000
Size: 639 8361
BsmI GAATG_Cn'
Cuts at: 0 2135 7731 9000
Size: 2135 5596 1269
Fragments arranged by size:
5596 2135 1269
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1274 3339 5416 6418 7871 8668 9000
Size: 1274 2065 2077 1002 1453 797 332
Fragments arranged by size:
2077 2065 1453 1274 1002 797 332
Bsp1286I G_dGCh'C
Cuts at: 0 614 792 1058 1355 1809 2097 2220 2781
Size: 614 178 266 297 454 288 123 561
Cuts at: 2781 2842 2947 3115 3149 3460 3653 4451 4860
Size: 61 105 168 34 311 193 798 409
Cuts at: 4860 4868 5288 5303 5402 5461 5768 5920 6116
Size: 8 420 15 99 59 307 152 196
Cuts at: 6116 6326 6410 6451 6694 6749 6792 7051 7097
Size: 210 84 41 243 55 43 259 46
Cuts at: 7097 7538 8032 8083 8210 8350 8548 8709 8825
Size: 441 494 51 127 140 198 161 116
Cuts at: 8825 9000
Size: 175
Fragments arranged by size:
798 614 561 494 454 441 420 409
311 307 297 288 266 259 243 210
198 196 193 178 175 168 161 152
140 127 123 116 105 99 84 61
59 55 51 46 43 41 34 15
8
BspEI T'CCGG_A
Cuts at: 0 8480 9000
Size: 8480 520
BspHI T'CATG_A
Cuts at: 0 8909 9000
Size: 8909 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 729 1065 4158 4460 5190 5480 7971 8237
Size: 729 336 3093 302 730 290 2491 266
Cuts at: 8237 8366 8614 8791 8854 8908 9000
Size: 129 248 177 63 54 92
Fragments arranged by size:
3093 2491 730 729 336 302 290 266
248 177 129 92 63 54
BsrBI CCG'CTC
Cuts at: 0 3604 3834 4034 9000
Size: 3604 230 200 4966
Fragments arranged by size:
4966 3604 230 200
BsrDI GCAATG_nn'
Cuts at: 0 1651 2431 3447 3701 3828 4542 9000
Size: 1651 780 1016 254 127 714 4458
Fragments arranged by size:
4458 1651 1016 780 714 254 127
BsrFI r'CCGG_y
Cuts at: 0 5476 6286 6373 8429 8536 9000
Size: 5476 810 87 2056 107 464
Fragments arranged by size:
5476 2056 810 464 107 87
BsrGI T'GTAC_A
Cuts at: 0 2102 4841 6455 9000
Size: 2102 2739 1614 2545
Fragments arranged by size:
2739 2545 2102 1614
BssSI C'ACGA_G
Cuts at: 0 222 9000
Size: 222 8778
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2215 6005 8109 8229 9000
Size: 2215 3790 2104 120 771
Fragments arranged by size:
3790 2215 2104 771 120
BstBI TT'CG_AA
Cuts at: 0 4008 9000
Size: 4008 4992
BstEII G'GTnAC_C
Cuts at: 0 5479 5583 7701 7897 9000
Size: 5479 104 2118 196 1103
Fragments arranged by size:
5479 2118 1103 196 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1138 4275 5241 5382 5967 7848 9000
Size: 1138 3137 966 141 585 1881 1152
Fragments arranged by size:
3137 1881 1152 1138 966 585 141
BstYI r'GATC_y
Cuts at: 0 1401 1560 2251 4682 5954 6038 6273 6529
Size: 1401 159 691 2431 1272 84 235 256
Cuts at: 6529 6956 9000
Size: 427 2044
Fragments arranged by size:
2431 2044 1401 1272 691 427 256 235
159 84
BstZ17I GTA'TAC
Cuts at: 0 7640 9000
Size: 7640 1360
Bsu36I CC'TnA_GG
Cuts at: 0 2498 3746 6961 7220 9000
Size: 2498 1248 3215 259 1780
Fragments arranged by size:
3215 2498 1780 1248 259
BtgI C'CryG_G
Cuts at: 0 974 3509 3593 4571 4852 4936 5234 5744
Size: 974 2535 84 978 281 84 298 510
Cuts at: 5744 6015 7493 7944 8339 9000
Size: 271 1478 451 395 661
Fragments arranged by size:
2535 1478 978 974 661 510 451 395
298 281 271 84 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 186 3734 5874 7635 9000
Size: 186 3548 2140 1761 1365
Fragments arranged by size:
3548 2140 1761 1365 186
BtsI GCAGTG_nn'
Cuts at: 0 882 2163 2968 4068 4194 8810 9000
Size: 882 1281 805 1100 126 4616 190
Fragments arranged by size:
4616 1281 1100 882 805 190 126
ClaI AT'CG_AT
Cuts at: 0 7352 9000
Size: 7352 1648
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2780 2815 9000
Size: 2780 35 6185
Fragments arranged by size:
6185 2780 35
DraIII CAC_nnn'GTG
Cuts at: 0 1118 1802 2215 4890 6333 7035 9000
Size: 1118 684 413 2675 1443 702 1965
Fragments arranged by size:
2675 1965 1443 1118 702 684 413
EaeI y'GGCC_r
Cuts at: 0 449 968 3135 3349 3406 3596 4340 4625
Size: 449 519 2167 214 57 190 744 285
Cuts at: 4625 4881 4902 5372 5474 5537 6919 7886 8336
Size: 256 21 470 102 63 1382 967 450
Cuts at: 8336 8525 9000
Size: 189 475
Fragments arranged by size:
2167 1382 967 744 519 475 470 450
449 285 256 214 190 189 102 63
57 21
EagI C'GGCC_G
Cuts at: 0 3135 3406 5474 6919 8525 9000
Size: 3135 271 2068 1445 1606 475
Fragments arranged by size:
3135 2068 1606 1445 475 271
EarI CTCTTCn'nnn_
Cuts at: 0 154 851 1565 1763 2444 3172 3467 3914
Size: 154 697 714 198 681 728 295 447
Cuts at: 3914 4417 4721 6632 6907 9000
Size: 503 304 1911 275 2093
Fragments arranged by size:
2093 1911 728 714 697 681 503 447
304 295 275 198 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2846 4557 5827 6192 7954 9000
Size: 2846 1711 1270 365 1762 1046
Fragments arranged by size:
2846 1762 1711 1270 1046 365
EcoICRI GAG'CTC
Cuts at: 0 1353 3651 5286 5400 9000
Size: 1353 2298 1635 114 3600
Fragments arranged by size:
3600 2298 1635 1353 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 921 1138 1279 1383 1586 1630 2164
Size: 673 248 217 141 104 203 44 534
Cuts at: 2164 2735 3144 4022 4465 4494 5075 5107 5329
Size: 571 409 878 443 29 581 32 222
Cuts at: 5329 5338 6958 7317 7414 7612 7684 8320 8608
Size: 9 1620 359 97 198 72 636 288
Cuts at: 8608 8731 8971 9000
Size: 123 240 29
Fragments arranged by size:
1620 878 673 636 581 571 534 443
409 359 288 248 240 222 217 203
198 141 123 104 97 72 44 32
29 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2537 4645 4918 5197 5971 7663 7978 8373
Size: 2537 2108 273 279 774 1692 315 395
Cuts at: 8373 9000
Size: 627
Fragments arranged by size:
2537 2108 1692 774 627 395 315 279
273
EcoO109I rG'GnC_Cy
Cuts at: 0 239 697 2982 3495 3863 4238 5856 5907
Size: 239 458 2285 513 368 375 1618 51
Cuts at: 5907 5917 7182 8304 8705 8706 8803 9000
Size: 10 1265 1122 401 1 97 197
Fragments arranged by size:
2285 1618 1265 1122 513 458 401 375
368 239 197 97 51 10 1
EcoRI G'AATT_C
Cuts at: 0 105 1539 2650 9000
Size: 105 1434 1111 6350
Fragments arranged by size:
6350 1434 1111 105
EcoRV GAT'ATC
Cuts at: 0 290 7284 8244 9000
Size: 290 6994 960 756
Fragments arranged by size:
6994 960 756 290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1560 3882 9000
Size: 1560 2322 5118
Fragments arranged by size:
5118 2322 1560
FseI GG_CCGG'CC
Cuts at: 0 8540 9000
Size: 8540 460
FspI TGC'GCA
Cuts at: 0 810 5293 7344 8602 9000
Size: 810 4483 2051 1258 398
Fragments arranged by size:
4483 2051 1258 810 398
FspAI rTGC'GCAy
Cuts at: 0 7344 9000
Size: 7344 1656
HaeII r_GCGC'y
Cuts at: 0 1315 1700 2924 3312 3485 5450 5730 5993
Size: 1315 385 1224 388 173 1965 280 263
Cuts at: 5993 6200 9000
Size: 207 2800
Fragments arranged by size:
2800 1965 1315 1224 388 385 280 263
207 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1615 1647 3240 3272 3823 3855 5605 5637
Size: 1615 32 1593 32 551 32 1750 32
Cuts at: 5637 5978 6010 6433 6465 6514 6546 7787 7819
Size: 341 32 423 32 49 32 1241 32
Cuts at: 7819 9000
Size: 1181
Fragments arranged by size:
1750 1615 1593 1241 1181 551 423 341
49 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 4755 6632 9000
Size: 4755 1877 2368
Fragments arranged by size:
4755 2368 1877
HindIII A'AGCT_T
Cuts at: 0 8917 9000
Size: 8917 83
KasI G'GCGC_C
Cuts at: 0 1696 5446 5989 9000
Size: 1696 3750 543 3011
Fragments arranged by size:
3750 3011 1696 543
MfeI C'AATT_G
Cuts at: 0 2113 3995 9000
Size: 2113 1882 5005
Fragments arranged by size:
5005 2113 1882
MluI A'CGCG_T
Cuts at: 0 4943 9000
Size: 4943 4057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1516 1707 4050 4211 5206 5375 6299 6419
Size: 1516 191 2343 161 995 169 924 120
Cuts at: 6419 7383 9000
Size: 964 1617
Fragments arranged by size:
2343 1617 1516 995 964 924 191 169
161 120
MscI TGG'CCA
Cuts at: 0 451 970 3351 3598 4342 4627 4904 5374
Size: 451 519 2381 247 744 285 277 470
Cuts at: 5374 7888 8338 9000
Size: 2514 450 662
Fragments arranged by size:
2514 2381 744 662 519 470 451 450
285 277 247
MslI CAynn'nnrTG
Cuts at: 0 3152 3440 4762 5239 5380 7472 7699 8440
Size: 3152 288 1322 477 141 2092 227 741
Cuts at: 8440 8890 8908 8978 9000
Size: 450 18 70 22
Fragments arranged by size:
3152 2092 1322 741 477 450 288 227
141 70 22 18
MspA1I CmG'CkG
Cuts at: 0 501 634 1010 1299 2674 4908 4938 4984
Size: 501 133 376 289 1375 2234 30 46
Cuts at: 4984 5121 5329 5358 6051 6279 7495 7747 7848
Size: 137 208 29 693 228 1216 252 101
Cuts at: 7848 7955 8041 8106 8127 8160 8565 9000
Size: 107 86 65 21 33 405 435
Fragments arranged by size:
2234 1375 1216 693 501 435 405 376
289 252 228 208 137 133 107 101
86 65 46 33 30 29 21
NaeI GCC'GGC
Cuts at: 0 6288 8431 8538 9000
Size: 6288 2143 107 462
Fragments arranged by size:
6288 2143 462 107
NarI GG'CG_CC
Cuts at: 0 1697 5447 5990 9000
Size: 1697 3750 543 3010
Fragments arranged by size:
3750 3010 1697 543
NcoI C'CATG_G
Cuts at: 0 974 3509 7944 9000
Size: 974 2535 4435 1056
Fragments arranged by size:
4435 2535 1056 974
NdeI CA'TA_TG
Cuts at: 0 7908 9000
Size: 7908 1092
NgoMIV G'CCGG_C
Cuts at: 0 6286 8429 8536 9000
Size: 6286 2143 107 464
Fragments arranged by size:
6286 2143 464 107
NruI TCG'CGA
Cuts at: 0 2847 9000
Size: 2847 6153
NsiI A_TGCA'T
Cuts at: 0 7351 9000
Size: 7351 1649
NspI r_CATG'y
Cuts at: 0 196 330 618 722 1981 2606 2877 3348
Size: 196 134 288 104 1259 625 271 471
Cuts at: 3348 4230 5799 7349 7366 8907 9000
Size: 882 1569 1550 17 1541 93
Fragments arranged by size:
1569 1550 1541 1259 882 625 471 288
271 196 134 104 93 17
PasI CC'CwG_GG
Cuts at: 0 6694 9000
Size: 6694 2306
PciI A'CATG_T
Cuts at: 0 718 4226 9000
Size: 718 3508 4774
Fragments arranged by size:
4774 3508 718
PflMI CCAn_nnn'nTGG
Cuts at: 0 980 1802 5053 5277 5910 8334 9000
Size: 980 822 3251 224 633 2424 666
Fragments arranged by size:
3251 2424 980 822 666 633 224
PfoI T'CCnGG_A
Cuts at: 0 743 1154 1467 1563 3261 4430 5052 5128
Size: 743 411 313 96 1698 1169 622 76
Cuts at: 5128 5950 9000
Size: 822 3050
Fragments arranged by size:
3050 1698 1169 822 743 622 411 313
96 76
PmlI CAC'GTG
Cuts at: 0 405 9000
Size: 405 8595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5422 5454 9000
Size: 5422 32 3546
Fragments arranged by size:
5422 3546 32
PpuMI rG'GwC_Cy
Cuts at: 0 239 697 5856 7182 8803 9000
Size: 239 458 5159 1326 1621 197
Fragments arranged by size:
5159 1621 1326 458 239 197
PshAI GACnn'nnGTC
Cuts at: 0 682 5188 9000
Size: 682 4506 3812
Fragments arranged by size:
4506 3812 682
PsiI TTA'TAA
Cuts at: 0 3641 9000
Size: 3641 5359
PspOMI G'GGCC_C
Cuts at: 0 5916 8705 9000
Size: 5916 2789 295
Fragments arranged by size:
5916 2789 295
PspXI vC'TCGA_Gb
Cuts at: 0 6292 8629 9000
Size: 6292 2337 371
Fragments arranged by size:
6292 2337 371
PstI C_TGCA'G
Cuts at: 0 443 1779 2001 4700 5201 6056 6232 7574
Size: 443 1336 222 2699 501 855 176 1342
Cuts at: 7574 8000 8234 8377 8507 8564 9000
Size: 426 234 143 130 57 436
Fragments arranged by size:
2699 1342 1336 855 501 443 436 426
234 222 176 143 130 57
PvuII CAG'CTG
Cuts at: 0 501 634 1299 2674 4908 5121 5358 7848
Size: 501 133 665 1375 2234 213 237 2490
Cuts at: 7848 8041 8106 8127 8565 9000
Size: 193 65 21 438 435
Fragments arranged by size:
2490 2234 1375 665 501 438 435 237
213 193 133 65 21
SacI G_AGCT'C
Cuts at: 0 1355 3653 5288 5402 9000
Size: 1355 2298 1635 114 3598
Fragments arranged by size:
3598 2298 1635 1355 114
SacII CC_GC'GG
Cuts at: 0 4939 7496 9000
Size: 4939 2557 1504
Fragments arranged by size:
4939 2557 1504
SanDI GG'GwC_CC
Cuts at: 0 239 8803 9000
Size: 239 8564 197
Fragments arranged by size:
8564 239 197
SapI GCTCTTCn'nnn_
Cuts at: 0 3172 9000
Size: 3172 5828
SbfI CC_TGCA'GG
Cuts at: 0 5201 8234 9000
Size: 5201 3033 766
Fragments arranged by size:
5201 3033 766
ScaI AGT'ACT
Cuts at: 0 8929 9000
Size: 8929 71
SfcI C'TryA_G
Cuts at: 0 439 480 1775 1841 1997 2333 2358 3068
Size: 439 41 1295 66 156 336 25 710
Cuts at: 3068 4696 5197 6052 6228 6656 7570 7996 8230
Size: 1628 501 855 176 428 914 426 234
Cuts at: 8230 8373 8503 8560 9000
Size: 143 130 57 440
Fragments arranged by size:
1628 1295 914 855 710 501 440 439
428 426 336 234 176 156 143 130
66 57 41 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 976 8533 9000
Size: 976 7557 467
Fragments arranged by size:
7557 976 467
SfoI GGC'GCC
Cuts at: 0 1698 5448 5991 9000
Size: 1698 3750 543 3009
Fragments arranged by size:
3750 3009 1698 543
SmaI CCC'GGG
Cuts at: 0 2941 4776 9000
Size: 2941 1835 4224
Fragments arranged by size:
4224 2941 1835
SmlI C'TyrA_G
Cuts at: 0 912 1185 6292 6683 7119 7377 8629 8638
Size: 912 273 5107 391 436 258 1252 9
Cuts at: 8638 9000
Size: 362
Fragments arranged by size:
5107 1252 912 436 391 362 273 258
9
SnaBI TAC'GTA
Cuts at: 0 5501 9000
Size: 5501 3499
SphI G_CATG'C
Cuts at: 0 2606 7349 9000
Size: 2606 4743 1651
Fragments arranged by size:
4743 2606 1651
StuI AGG'CCT
Cuts at: 0 1817 2933 5710 6004 6220 7375 9000
Size: 1817 1116 2777 294 216 1155 1625
Fragments arranged by size:
2777 1817 1625 1155 1116 294 216
StyI C'CwwG_G
Cuts at: 0 974 1739 3275 3509 3788 7079 7772 7944
Size: 974 765 1536 234 279 3291 693 172
Cuts at: 7944 8210 9000
Size: 266 790
Fragments arranged by size:
3291 1536 974 790 765 693 279 266
234 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 146 940 2364 2825 5977 6967 7283 7647
Size: 146 794 1424 461 3152 990 316 364
Cuts at: 7647 8308 8737 9000
Size: 661 429 263
Fragments arranged by size:
3152 1424 990 794 661 461 429 364
316 263 146
TatI w'GTAC_w
Cuts at: 0 2102 2469 3287 4349 4841 6455 7004 7418
Size: 2102 367 818 1062 492 1614 549 414
Cuts at: 7418 8927 9000
Size: 1509 73
Fragments arranged by size:
2102 1614 1509 1062 818 549 492 414
367 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 889 921 2514 2546 2728 2760 4472 4504
Size: 889 32 1593 32 182 32 1712 32
Cuts at: 4504 5785 5817 6116 6148 6319 6351 8293 8325
Size: 1281 32 299 32 171 32 1942 32
Cuts at: 8325 8719 8751 9000
Size: 394 32 249
Fragments arranged by size:
1942 1712 1593 1281 889 394 299 249
182 171 32 32 32 32 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 927 5020 9000
Size: 927 4093 3980
Fragments arranged by size:
4093 3980 927
XbaI T'CTAG_A
Cuts at: 0 2089 2404 9000
Size: 2089 315 6596
Fragments arranged by size:
6596 2089 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 751 4272 4755 8658 9000
Size: 751 3521 483 3903 342
Fragments arranged by size:
3903 3521 751 483 342
XhoI C'TCGA_G
Cuts at: 0 6292 8629 9000
Size: 6292 2337 371
Fragments arranged by size:
6292 2337 371
XmaI C'CCGG_G
Cuts at: 0 2939 4774 9000
Size: 2939 1835 4226
Fragments arranged by size:
4226 2939 1835
XmnI GAAnn'nnTTC
Cuts at: 0 2135 2367 6641 7247 9000
Size: 2135 232 4274 606 1753
Fragments arranged by size:
4274 2135 1753 606 232
ZraI GAC'GTC
Cuts at: 0 3333 8154 9000
Size: 3333 4821 846
Fragments arranged by size:
4821 3333 846
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII KasI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuII SacI SacII SanDI
SapI SbfI ScaI SfcI SfiI SfoI SmaI SmlI
SnaBI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AhdI AscI AseI AsiSI AvrII
BaeI BmtI BssHII DraI DrdI HpaI KpnI NheI
NotI PacI PmeI PsrI PvuI RsrII SalI SexAI
SgfI SgrAI SpeI SrfI SspI SwaI