Restriction map for
pF1KA0619
(Linear) (Six-base) MAPSORT of: KIAA0619.seq Check: 1191 from: 1 to: 4164
KIAA0619.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 252 1348 4137 4164
Size: 252 1096 2789 27
Fragments arranged by size:
2789 1096 252 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 968 1507 1852 1999 2449 2816 3308
Size: 608 360 539 345 147 450 367 492
Cuts at: 3308 4164
Size: 856
Fragments arranged by size:
856 608 539 492 450 367 360 345
147
AfeI AGC'GCT
Cuts at: 0 2407 4164
Size: 2407 1757
AflIII A'CryG_T
Cuts at: 0 703 3848 4164
Size: 703 3145 316
Fragments arranged by size:
3145 703 316
AleI CACnn'nnGTG
Cuts at: 0 3163 4164
Size: 3163 1001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1393 1550 4164
Size: 1393 157 2614
Fragments arranged by size:
2614 1393 157
AlwNI CAG_nnn'CTG
Cuts at: 0 400 1108 1723 1782 2770 3690 4164
Size: 400 708 615 59 988 920 474
Fragments arranged by size:
988 920 708 615 474 400 59
ApoI r'AATT_y
Cuts at: 0 150 574 930 959 1254 1581 1855 3499
Size: 150 424 356 29 295 327 274 1644
Cuts at: 3499 3668 3727 3993 4164
Size: 169 59 266 171
Fragments arranged by size:
1644 424 356 327 295 274 266 171
169 150 59 29
AseI AT'TA_AT
Cuts at: 0 1922 4164
Size: 1922 2242
AvaI C'yCGr_G
Cuts at: 0 40 4164
Size: 40 4124
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 734 767 4164
Size: 734 33 3397
Fragments arranged by size:
3397 734 33
BanI G'GyrC_C
Cuts at: 0 34 4164
Size: 34 4130
BanII G_rGCy'C
Cuts at: 0 2333 2585 4164
Size: 2333 252 1579
Fragments arranged by size:
2333 1579 252
BbeI G_GCGC'C
Cuts at: 0 38 4164
Size: 38 4126
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 4164
Size: 275 3889
BbvCI CC'TCA_GC
Cuts at: 0 3425 4164
Size: 3425 739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 341 1131 1165 2764 2798 4164
Size: 307 34 790 34 1599 34 1366
Fragments arranged by size:
1599 1366 790 307 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 864 4164
Size: 864 3300
BclI T'GATC_A
Cuts at: 0 1068 1557 3242 4164
Size: 1068 489 1685 922
Fragments arranged by size:
1685 1068 922 489
BglI GCCn_nnn'nGGC
Cuts at: 0 2134 3843 4164
Size: 2134 1709 321
Fragments arranged by size:
2134 1709 321
BglII A'GATC_T
Cuts at: 0 1800 2159 4164
Size: 1800 359 2005
Fragments arranged by size:
2005 1800 359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1663 1846 2020 2569 2764 2863 4046
Size: 109 1554 183 174 549 195 99 1183
Cuts at: 4046 4164
Size: 118
Fragments arranged by size:
1554 1183 549 195 183 174 118 109
99
Bpu10I CC'TnA_GC
Cuts at: 0 3425 4164
Size: 3425 739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2932 3080 4164
Size: 2932 148 1084
Fragments arranged by size:
2932 1084 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 98 518 4164
Size: 37 61 420 3646
Fragments arranged by size:
3646 420 61 37
BsaAI yAC'GTr
Cuts at: 0 704 4164
Size: 704 3460
BsaBI GATnn'nnATC
Cuts at: 0 488 4164
Size: 488 3676
BsaHI Gr'CG_yC
Cuts at: 0 35 4164
Size: 35 4129
BsaWI w'CCGG_w
Cuts at: 0 759 4164
Size: 759 3405
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 97 127 515 545 1089 1119 4164
Size: 97 30 388 30 544 30 3045
Fragments arranged by size:
3045 544 388 97 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1471 3851 3940 4164
Size: 1471 2380 89 224
Fragments arranged by size:
2380 1471 224 89
BseYI C'CCAG_C
Cuts at: 0 3233 4056 4164
Size: 3233 823 108
Fragments arranged by size:
3233 823 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 4164
Size: 337 3827
BsiHKAI G_wGCw'C
Cuts at: 0 2333 2585 4164
Size: 2333 252 1579
Fragments arranged by size:
2333 1579 252
Bsp1286I G_dGCh'C
Cuts at: 0 2333 2585 4164
Size: 2333 252 1579
Fragments arranged by size:
2333 1579 252
BspHI T'CATG_A
Cuts at: 0 1560 2831 3122 4164
Size: 1560 1271 291 1042
Fragments arranged by size:
1560 1271 1042 291
BsrDI GCAATG_nn'
Cuts at: 0 1377 3472 4164
Size: 1377 2095 692
Fragments arranged by size:
2095 1377 692
BsrFI r'CCGG_y
Cuts at: 0 5 4164
Size: 5 4159
BsrGI T'GTAC_A
Cuts at: 0 494 4164
Size: 494 3670
BstBI TT'CG_AA
Cuts at: 0 2544 3303 4164
Size: 2544 759 861
Fragments arranged by size:
2544 861 759
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 4164
Size: 126 4038
BstYI r'GATC_y
Cuts at: 0 1800 2159 4164
Size: 1800 359 2005
Fragments arranged by size:
2005 1800 359
BstZ17I GTA'TAC
Cuts at: 0 1349 4164
Size: 1349 2815
Bsu36I CC'TnA_GG
Cuts at: 0 776 4164
Size: 776 3388
BtgI C'CryG_G
Cuts at: 0 620 1905 4164
Size: 620 1285 2259
Fragments arranged by size:
2259 1285 620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 323 4164
Size: 323 3841
BtsI GCAGTG_nn'
Cuts at: 0 581 1134 3414 4164
Size: 581 553 2280 750
Fragments arranged by size:
2280 750 581 553
DraI TTT'AAA
Cuts at: 0 1964 2237 4164
Size: 1964 273 1927
Fragments arranged by size:
1964 1927 273
DrdI GACnn_nn'nnGTC
Cuts at: 0 4133 4164
Size: 4133 31
EaeI y'GGCC_r
Cuts at: 0 4129 4164
Size: 4129 35
EarI CTCTTCn'nnn_
Cuts at: 0 291 1448 1999 3284 3998 4164
Size: 291 1157 551 1285 714 166
Fragments arranged by size:
1285 1157 714 551 291 166
EcoICRI GAG'CTC
Cuts at: 0 2331 2583 4164
Size: 2331 252 1581
Fragments arranged by size:
2331 1581 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 608 968 1507 1663 1846 1852 1999
Size: 109 499 360 539 156 183 6 147
Cuts at: 1999 2020 2449 2569 2764 2816 2863 3308 4046
Size: 21 429 120 195 52 47 445 738
Cuts at: 4046 4164
Size: 118
Fragments arranged by size:
738 539 499 445 429 360 195 183
156 147 120 118 109 52 47 21
6
EcoRI G'AATT_C
Cuts at: 0 930 4164
Size: 930 3234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 320 613 1381 1633 2235 3484 4164
Size: 320 293 768 252 602 1249 680
Fragments arranged by size:
1249 768 680 602 320 293 252
HaeII r_GCGC'y
Cuts at: 0 38 97 1519 2409 4164
Size: 38 59 1422 890 1755
Fragments arranged by size:
1755 1422 890 59 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 754 786 817 849 1261 1293
Size: 97 32 625 32 31 32 412 32
Cuts at: 1293 2992 3024 3111 3143 3196 3228 3234 3266
Size: 1699 32 87 32 53 32 6 32
Cuts at: 3266 4164
Size: 898
Fragments arranged by size:
1699 898 625 412 97 87 53 32
32 32 32 32 32 32 32 31
6
HincII GTy'rAC
Cuts at: 0 1599 2451 4138 4164
Size: 1599 852 1687 26
Fragments arranged by size:
1687 1599 852 26
HindIII A'AGCT_T
Cuts at: 0 361 1202 4164
Size: 361 841 2962
Fragments arranged by size:
2962 841 361
HpaI GTT'AAC
Cuts at: 0 1599 2451 4164
Size: 1599 852 1713
Fragments arranged by size:
1713 1599 852
KasI G'GCGC_C
Cuts at: 0 34 4164
Size: 34 4130
MfeI C'AATT_G
Cuts at: 0 2789 2900 4164
Size: 2789 111 1264
Fragments arranged by size:
2789 1264 111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1191 3648 3726 4164
Size: 732 459 2457 78 438
Fragments arranged by size:
2457 732 459 438 78
MslI CAynn'nnrTG
Cuts at: 0 124 502 638 706 1532 2506 3163 4164
Size: 124 378 136 68 826 974 657 1001
Fragments arranged by size:
1001 974 826 657 378 136 124 68
MspA1I CmG'CkG
Cuts at: 0 1221 1782 3354 4164
Size: 1221 561 1572 810
Fragments arranged by size:
1572 1221 810 561
NaeI GCC'GGC
Cuts at: 0 7 4164
Size: 7 4157
NarI GG'CG_CC
Cuts at: 0 35 4164
Size: 35 4129
NcoI C'CATG_G
Cuts at: 0 620 1905 4164
Size: 620 1285 2259
Fragments arranged by size:
2259 1285 620
NdeI CA'TA_TG
Cuts at: 0 3200 3911 4164
Size: 3200 711 253
Fragments arranged by size:
3200 711 253
NgoMIV G'CCGG_C
Cuts at: 0 5 4164
Size: 5 4159
NspI r_CATG'y
Cuts at: 0 516 660 973 3852 4164
Size: 516 144 313 2879 312
Fragments arranged by size:
2879 516 313 312 144
PasI CC'CwG_GG
Cuts at: 0 449 4164
Size: 449 3715
PciI A'CATG_T
Cuts at: 0 3848 4164
Size: 3848 316
PflMI CCAn_nnn'nTGG
Cuts at: 0 2502 4164
Size: 2502 1662
PfoI T'CCnGG_A
Cuts at: 0 2585 4164
Size: 2585 1579
PmlI CAC'GTG
Cuts at: 0 704 4164
Size: 704 3460
PsiI TTA'TAA
Cuts at: 0 2627 4164
Size: 2627 1537
PstI C_TGCA'G
Cuts at: 0 1734 3317 4164
Size: 1734 1583 847
Fragments arranged by size:
1734 1583 847
PvuII CAG'CTG
Cuts at: 0 1221 1782 3354 4164
Size: 1221 561 1572 810
Fragments arranged by size:
1572 1221 810 561
SacI G_AGCT'C
Cuts at: 0 2333 2585 4164
Size: 2333 252 1579
Fragments arranged by size:
2333 1579 252
SalI G'TCGA_C
Cuts at: 0 4136 4164
Size: 4136 28
SapI GCTCTTCn'nnn_
Cuts at: 0 3998 4164
Size: 3998 166
SfcI C'TryA_G
Cuts at: 0 253 836 1109 1245 1730 3313 3998 4164
Size: 253 583 273 136 485 1583 685 166
Fragments arranged by size:
1583 685 583 485 273 253 166 136
SfoI GGC'GCC
Cuts at: 0 36 4164
Size: 36 4128
SmlI C'TyrA_G
Cuts at: 0 2911 3095 4164
Size: 2911 184 1069
Fragments arranged by size:
2911 1069 184
SpeI A'CTAG_T
Cuts at: 0 2935 4164
Size: 2935 1229
SspI AAT'ATT
Cuts at: 0 2806 4164
Size: 2806 1358
StyI C'CwwG_G
Cuts at: 0 155 620 1905 3673 4164
Size: 155 465 1285 1768 491
Fragments arranged by size:
1768 1285 491 465 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 441 4164
Size: 441 3723
TatI w'GTAC_w
Cuts at: 0 494 509 4164
Size: 494 15 3655
Fragments arranged by size:
3655 494 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 926 958 1390 1422 4164
Size: 926 32 432 32 2742
Fragments arranged by size:
2742 926 432 32 32
XbaI T'CTAG_A
Cuts at: 0 1878 4164
Size: 1878 2286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1392 2202 3588 4164
Size: 1392 810 1386 576
Fragments arranged by size:
1392 1386 810 576
XmnI GAAnn'nnTTC
Cuts at: 0 593 4164
Size: 593 3571
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApoI
AseI AvaI BaeI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BsrGI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EarI EcoICRI Eco57MI EcoRI FalI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MmeI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PsiI PstI PvuII SacI
SalI SapI SfcI SfoI SmlI SpeI SspI StyI
TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaI ApaLI AscI AsiSI AvrII BamHI BfrBI BlpI
Bme1580I BmgBI BmrI BmtI BplI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BssHII BssSI BstAPI BstEII
ClaI CspCI DraIII EagI EciI EcoNI EcoO109I EcoRV
FseI FspI FspAI KpnI MluI MscI NheI NotI
NruI NsiI PacI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI Tth111I XhoI XmaI ZraI