Restriction map for
pF1KSDA0620
(Linear) (Six-base) MAPSORT of: KIAA0620.seq Check: 3699 from: 1 to: 5775
KIAA0620.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1355 1500 2668 3376 5715 5775
Size: 1355 145 1168 708 2339 60
Fragments arranged by size:
2339 1355 1168 708 145 60
AatII G_ACGT'C
Cuts at: 0 1612 4817 5775
Size: 1612 3205 958
Fragments arranged by size:
3205 1612 958
AccI GT'mk_AC
Cuts at: 0 4817 5775
Size: 4817 958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1399 4955 5347 5775
Size: 1399 3556 392 428
Fragments arranged by size:
3556 1399 428 392
AfeI AGC'GCT
Cuts at: 0 5509 5775
Size: 5509 266
AflIII A'CryG_T
Cuts at: 0 563 887 4676 5775
Size: 563 324 3789 1099
Fragments arranged by size:
3789 1099 563 324
AgeI A'CCGG_T
Cuts at: 0 640 5775
Size: 640 5135
AleI CACnn'nnGTG
Cuts at: 0 1390 1417 2195 4879 5611 5775
Size: 1390 27 778 2684 732 164
Fragments arranged by size:
2684 1390 778 732 164 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 634 982 1876 2005 3859 4798 5107 5775
Size: 634 348 894 129 1854 939 309 668
Fragments arranged by size:
1854 939 894 668 634 348 309 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5146 5178 5775
Size: 5146 32 597
Fragments arranged by size:
5146 597 32
AlwNI CAG_nnn'CTG
Cuts at: 0 405 1377 1796 1954 2577 3042 3268 4462
Size: 405 972 419 158 623 465 226 1194
Cuts at: 4462 5775
Size: 1313
Fragments arranged by size:
1313 1194 972 623 465 419 405 226
158
ApaI G_GGCC'C
Cuts at: 0 289 1789 2974 3120 3387 5775
Size: 289 1500 1185 146 267 2388
Fragments arranged by size:
2388 1500 1185 289 267 146
ApaLI G'TGCA_C
Cuts at: 0 875 1555 1651 1722 3169 5775
Size: 875 680 96 71 1447 2606
Fragments arranged by size:
2606 1447 875 680 96 71
AvaI C'yCGr_G
Cuts at: 0 38 706 1091 1404 1869 1940 2671 2915
Size: 38 668 385 313 465 71 731 244
Cuts at: 2915 3379 3475 3571 5775
Size: 464 96 96 2204
Fragments arranged by size:
2204 731 668 465 464 385 313 244
96 96 71 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2107 2140 5775
Size: 2107 33 3635
Fragments arranged by size:
3635 2107 33
BanI G'GyrC_C
Cuts at: 0 83 90 133 250 291 353 452 628
Size: 83 7 43 117 41 62 99 176
Cuts at: 628 808 976 1233 2280 2316 2656 2986 3132
Size: 180 168 257 1047 36 340 330 146
Cuts at: 3132 4939 5775
Size: 1807 836
Fragments arranged by size:
1807 1047 836 340 330 257 180 176
168 146 117 99 83 62 43 41
36 7
BanII G_rGCy'C
Cuts at: 0 289 515 1553 1789 2245 2522 2693 2974
Size: 289 226 1038 236 456 277 171 281
Cuts at: 2974 3035 3120 3332 3387 3623 4585 5057 5775
Size: 61 85 212 55 236 962 472 718
Fragments arranged by size:
1038 962 718 472 456 289 281 277
236 236 226 212 171 85 61 55
BbeI G_GCGC'C
Cuts at: 0 137 254 357 456 632 812 980 1237
Size: 137 117 103 99 176 180 168 257
Cuts at: 1237 5775
Size: 4538
Fragments arranged by size:
4538 257 180 176 168 137 117 103
99
BbsI GAAGACnn'nnnn_
Cuts at: 0 1074 1104 1446 2594 3867 3922 4895 5195
Size: 1074 30 342 1148 1273 55 973 300
Cuts at: 5195 5293 5775
Size: 98 482
Fragments arranged by size:
1273 1148 1074 973 482 342 300 98
55 30
BbvCI CC'TCA_GC
Cuts at: 0 3458 5775
Size: 3458 2317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3957 3991 5062 5096 5775
Size: 3957 34 1071 34 679
Fragments arranged by size:
3957 1071 679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5191 5775
Size: 5191 584
BglI GCCn_nnn'nGGC
Cuts at: 0 277 1892 3124 4948 5569 5775
Size: 277 1615 1232 1824 621 206
Fragments arranged by size:
1824 1615 1232 621 277 206
BglII A'GATC_T
Cuts at: 0 1865 2381 4002 5775
Size: 1865 516 1621 1773
Fragments arranged by size:
1865 1773 1621 516
BlpI GC'TnA_GC
Cuts at: 0 2470 3899 3980 4708 5542 5775
Size: 2470 1429 81 728 834 233
Fragments arranged by size:
2470 1429 834 728 233 81
Bme1580I G_kGCm'C
Cuts at: 0 88 289 296 879 1276 1465 1559 1655
Size: 88 201 7 583 397 189 94 96
Cuts at: 1655 1710 1726 1789 2974 2989 3120 3173 3387
Size: 55 16 63 1185 15 131 53 214
Cuts at: 3387 4787 5137 5775
Size: 1400 350 638
Fragments arranged by size:
1400 1185 638 583 397 350 214 201
189 131 96 94 88 63 55 53
16 15 7
BmgBI CAC'GTC
Cuts at: 0 1185 1242 2766 3895 5775
Size: 1185 57 1524 1129 1880
Fragments arranged by size:
1880 1524 1185 1129 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 2565 4788 4993 5331 5775
Size: 2565 2223 205 338 444
Fragments arranged by size:
2565 2223 444 338 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 286 700 1297 1732 2377 2395 2977
Size: 160 126 414 597 435 645 18 582
Cuts at: 2977 3019 3607 4066 4309 4387 4783 4821 5251
Size: 42 588 459 243 78 396 38 430
Cuts at: 5251 5695 5775
Size: 444 80
Fragments arranged by size:
645 597 588 582 459 444 435 430
414 396 243 160 126 80 78 42
38 18
Bpu10I CC'TnA_GC
Cuts at: 0 31 259 2516 3388 3458 5775
Size: 31 228 2257 872 70 2317
Fragments arranged by size:
2317 2257 872 228 70 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1468 4843 5775
Size: 778 690 3375 932
Fragments arranged by size:
3375 932 778 690
BsaAI yAC'GTr
Cuts at: 0 635 2034 2934 4113 5775
Size: 635 1399 900 1179 1662
Fragments arranged by size:
1662 1399 1179 900 635
BsaBI GATnn'nnATC
Cuts at: 0 715 2998 4495 5775
Size: 715 2283 1497 1280
Fragments arranged by size:
2283 1497 1280 715
BsaHI Gr'CG_yC
Cuts at: 0 73 134 251 354 453 629 809 977
Size: 73 61 117 103 99 176 180 168
Cuts at: 977 989 1234 1609 1688 4415 4592 4814 5375
Size: 12 245 375 79 2727 177 222 561
Cuts at: 5375 5411 5536 5775
Size: 36 125 239
Fragments arranged by size:
2727 561 375 245 239 222 180 177
176 168 125 117 103 99 79 73
61 36 12
BsaWI w'CCGG_w
Cuts at: 0 640 3129 5306 5492 5775
Size: 640 2489 2177 186 283
Fragments arranged by size:
2489 2177 640 283 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 1639 1669 1705 1735 2827 2857
Size: 637 30 972 30 36 30 1092 30
Cuts at: 2857 2896 2911 2926 2941 4567 4597 5401 5431
Size: 39 15 15 15 1626 30 804 30
Cuts at: 5431 5775
Size: 344
Fragments arranged by size:
1626 1092 972 804 637 344 39 36
30 30 30 30 30 30 15 15
15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2392 2848 3475 3493 3949 4230 4642 5248
Size: 2392 456 627 18 456 281 412 606
Cuts at: 5248 5455 5590 5775
Size: 207 135 185
Fragments arranged by size:
2392 627 606 456 456 412 281 207
185 135 18
BseYI C'CCAG_C
Cuts at: 0 529 828 1086 1578 1789 1882 3792 4176
Size: 529 299 258 492 211 93 1910 384
Cuts at: 4176 5436 5775
Size: 1260 339
Fragments arranged by size:
1910 1260 529 492 384 339 299 258
211 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1288 1725 2099 2199 2369 2422 3048
Size: 835 453 437 374 100 170 53 626
Cuts at: 3048 3084 3310 4339 4344 4826 5524 5775
Size: 36 226 1029 5 482 698 251
Fragments arranged by size:
1029 835 698 626 482 453 437 374
251 226 170 100 53 36 5
BsiEI CG_ry'CG
Cuts at: 0 224 281 473 1157 3728 4424 4896 5656
Size: 224 57 192 684 2571 696 472 760
Cuts at: 5656 5775
Size: 119
Fragments arranged by size:
2571 760 696 684 472 224 192 119
57
BsiHKAI G_wGCw'C
Cuts at: 0 347 879 1256 1559 1655 1726 3035 3173
Size: 347 532 377 303 96 71 1309 138
Cuts at: 3173 5096 5775
Size: 1923 679
Fragments arranged by size:
1923 1309 679 532 377 347 303 138
96 71
BsmI GAATG_Cn'
Cuts at: 0 5565 5775
Size: 5565 210
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1708 3799 4516 5775
Size: 1708 2091 717 1259
Fragments arranged by size:
2091 1708 1259 717
Bsp1286I G_dGCh'C
Cuts at: 0 88 289 296 347 515 879 1256 1276
Size: 88 201 7 51 168 364 377 20
Cuts at: 1276 1465 1553 1559 1655 1710 1726 1789 2245
Size: 189 88 6 96 55 16 63 456
Cuts at: 2245 2522 2693 2974 2989 3035 3120 3173 3332
Size: 277 171 281 15 46 85 53 159
Cuts at: 3332 3387 3623 4585 4787 5057 5096 5137 5775
Size: 55 236 962 202 270 39 41 638
Fragments arranged by size:
962 638 456 377 364 281 277 270
236 202 201 189 171 168 159 96
88 88 85 63 55 55 53 51
46 41 39 20 16 15 7 6
BspHI T'CATG_A
Cuts at: 0 4385 5775
Size: 4385 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1355 1500 2668 3376 3488 5438 5715 5775
Size: 1355 145 1168 708 112 1950 277 60
Fragments arranged by size:
1950 1355 1168 708 277 145 112 60
BsrBI CCG'CTC
Cuts at: 0 722 1098 1119 2469 5541 5775
Size: 722 376 21 1350 3072 234
Fragments arranged by size:
3072 1350 722 376 234 21
BsrFI r'CCGG_y
Cuts at: 0 130 640 979 1230 1281 2750 3123 4205
Size: 130 510 339 251 51 1469 373 1082
Cuts at: 4205 5069 5775
Size: 864 706
Fragments arranged by size:
1469 1082 864 706 510 373 339 251
130 51
BsrGI T'GTAC_A
Cuts at: 0 4505 5775
Size: 4505 1270
BssHII G'CGCG_C
Cuts at: 0 913 915 955 1207 5775
Size: 913 2 40 252 4568
Fragments arranged by size:
4568 913 252 40 2
BssSI C'ACGA_G
Cuts at: 0 336 622 2238 3631 5775
Size: 336 286 1616 1393 2144
Fragments arranged by size:
2144 1616 1393 336 286
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1217 3098 5775
Size: 1217 1881 2677
Fragments arranged by size:
2677 1881 1217
BstEII G'GTnAC_C
Cuts at: 0 1963 2596 2883 5775
Size: 1963 633 287 2892
Fragments arranged by size:
2892 1963 633 287
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3264 3319 4693 5775
Size: 3264 55 1374 1082
Fragments arranged by size:
3264 1374 1082 55
BstYI r'GATC_y
Cuts at: 0 143 377 1865 2381 2675 3950 4002 4757
Size: 143 234 1488 516 294 1275 52 755
Cuts at: 4757 4928 5522 5775
Size: 171 594 253
Fragments arranged by size:
1488 1275 755 594 516 294 253 234
171 143 52
Bsu36I CC'TnA_GG
Cuts at: 0 2246 3110 5775
Size: 2246 864 2665
Fragments arranged by size:
2665 2246 864
BtgI C'CryG_G
Cuts at: 0 14 79 281 349 473 572 596 972
Size: 14 65 202 68 124 99 24 376
Cuts at: 972 1050 1136 1418 1424 2549 2648 2772 2966
Size: 78 86 282 6 1125 99 124 194
Cuts at: 2966 3003 3302 3728 3857 4721 5618 5687 5775
Size: 37 299 426 129 864 897 69 88
Fragments arranged by size:
1125 897 864 426 376 299 282 202
194 129 124 124 99 99 88 86
78 69 68 65 37 24 14 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3078 3762 4332 5065 5376 5775
Size: 3078 684 570 733 311 399
Fragments arranged by size:
3078 733 684 570 399 311
BtsI GCAGTG_nn'
Cuts at: 0 1036 2111 2330 2806 3696 3784 4171 5775
Size: 1036 1075 219 476 890 88 387 1604
Fragments arranged by size:
1604 1075 1036 890 476 387 219 88
ClaI AT'CG_AT
Cuts at: 0 1829 5775
Size: 1829 3946
DraIII CAC_nnn'GTG
Cuts at: 0 2778 3288 4062 5775
Size: 2778 510 774 1713
Fragments arranged by size:
2778 1713 774 510
EaeI y'GGCC_r
Cuts at: 0 221 278 396 470 476 548 746 1154
Size: 221 57 118 74 6 72 198 408
Cuts at: 1154 1421 1427 1622 2324 2491 3215 3305 3321
Size: 267 6 195 702 167 724 90 16
Cuts at: 3321 3539 3725 3809 4421 4798 4893 4949 5570
Size: 218 186 84 612 377 95 56 621
Cuts at: 5570 5615 5653 5666 5775
Size: 45 38 13 109
Fragments arranged by size:
724 702 621 612 408 377 267 221
218 198 195 186 167 118 109 95
90 84 74 72 57 56 45 38
16 13 6 6
EagI C'GGCC_G
Cuts at: 0 221 278 470 1154 3725 4421 4893 5653
Size: 221 57 192 684 2571 696 472 760
Cuts at: 5653 5775
Size: 122
Fragments arranged by size:
2571 760 696 684 472 221 192 122
57
EarI CTCTTCn'nnn_
Cuts at: 0 760 2446 4097 4214 5242 5775
Size: 760 1686 1651 117 1028 533
Fragments arranged by size:
1686 1651 1028 760 533 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1699 1997 2082 3493 5065 5775
Size: 1699 298 85 1411 1572 710
Fragments arranged by size:
1699 1572 1411 710 298 85
EcoICRI GAG'CTC
Cuts at: 0 3033 5775
Size: 3033 2742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 286 700 1297 1399 1732 2377 2395
Size: 160 126 414 597 102 333 645 18
Cuts at: 2395 2977 3019 3607 4066 4309 4387 4783 4821
Size: 582 42 588 459 243 78 396 38
Cuts at: 4821 4955 5251 5347 5695 5775
Size: 134 296 96 348 80
Fragments arranged by size:
645 597 588 582 459 414 396 348
333 296 243 160 134 126 102 96
80 78 42 38 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5164 5775
Size: 5164 611
EcoO109I rG'GnC_Cy
Cuts at: 0 402 1130 1785 1786 1944 1951 2635 2717
Size: 402 728 655 1 158 7 684 82
Cuts at: 2717 2971 3039 3116 3117 3383 3384 4863 5775
Size: 254 68 77 1 266 1 1479 912
Fragments arranged by size:
1479 912 728 684 655 402 266 254
158 82 77 68 7 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 849 5775
Size: 849 4926
FspI TGC'GCA
Cuts at: 0 3079 5775
Size: 3079 2696
FspAI rTGC'GCAy
Cuts at: 0 3079 5775
Size: 3079 2696
HaeII r_GCGC'y
Cuts at: 0 38 137 254 357 456 632 812 845
Size: 38 99 117 103 99 176 180 33
Cuts at: 845 901 980 1237 3406 5511 5775
Size: 56 79 257 2169 2105 264
Fragments arranged by size:
2169 2105 264 257 180 176 117 103
99 99 79 56 38 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 1181 1213 2896 2911 2928 2943
Size: 135 32 1014 32 1683 15 17 15
Cuts at: 2943 3928 3930 3947 3960 3962 3979 4861 4893
Size: 985 2 17 13 2 17 882 32
Cuts at: 4893 5401 5433 5775
Size: 508 32 342
Fragments arranged by size:
1683 1014 985 882 508 342 135 32
32 32 32 17 17 17 15 15
13 2 2
HincII GTy'rAC
Cuts at: 0 228 1437 1470 3708 4818 5775
Size: 228 1209 33 2238 1110 957
Fragments arranged by size:
2238 1209 1110 957 228 33
HindIII A'AGCT_T
Cuts at: 0 4903 5775
Size: 4903 872
KasI G'GCGC_C
Cuts at: 0 133 250 353 452 628 808 976 1233
Size: 133 117 103 99 176 180 168 257
Cuts at: 1233 5775
Size: 4542
Fragments arranged by size:
4542 257 180 176 168 133 117 103
99
MluI A'CGCG_T
Cuts at: 0 563 887 5775
Size: 563 324 4888
Fragments arranged by size:
4888 563 324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 884 2228 2502 2687 2810 5285 5775
Size: 803 81 1344 274 185 123 2475 490
Fragments arranged by size:
2475 1344 803 490 274 185 123 81
MscI TGG'CCA
Cuts at: 0 748 1429 1624 2326 4951 5617 5775
Size: 748 681 195 702 2625 666 158
Fragments arranged by size:
2625 748 702 681 666 195 158
MslI CAynn'nnrTG
Cuts at: 0 1390 1417 1510 1900 2195 2314 3008 3262
Size: 1390 27 93 390 295 119 694 254
Cuts at: 3262 3817 4501 4879 5611 5775
Size: 555 684 378 732 164
Fragments arranged by size:
1390 732 694 684 555 390 378 295
254 164 119 93 27
MspA1I CmG'CkG
Cuts at: 0 16 81 96 243 306 324 351 387
Size: 16 65 15 147 63 18 27 36
Cuts at: 387 447 598 1052 1138 1326 1359 1450 1796
Size: 60 151 454 86 188 33 91 346
Cuts at: 1796 2147 3489 3738 3792 4511 5436 5775
Size: 351 1342 249 54 719 925 339
Fragments arranged by size:
1342 925 719 454 351 346 339 249
188 151 147 91 86 65 63 60
54 36 33 27 18 16 15
NaeI GCC'GGC
Cuts at: 0 132 981 2752 3125 4207 5775
Size: 132 849 1771 373 1082 1568
Fragments arranged by size:
1771 1568 1082 849 373 132
NarI GG'CG_CC
Cuts at: 0 134 251 354 453 629 809 977 1234
Size: 134 117 103 99 176 180 168 257
Cuts at: 1234 5775
Size: 4541
Fragments arranged by size:
4541 257 180 176 168 134 117 103
99
NcoI C'CATG_G
Cuts at: 0 2549 2648 2966 3003 3302 4721 5618 5775
Size: 2549 99 318 37 299 1419 897 157
Fragments arranged by size:
2549 1419 897 318 299 157 99 37
NgoMIV G'CCGG_C
Cuts at: 0 130 979 2750 3123 4205 5775
Size: 130 849 1771 373 1082 1570
Fragments arranged by size:
1771 1570 1082 849 373 130
NspI r_CATG'y
Cuts at: 0 537 1509 2610 3198 4506 5775
Size: 537 972 1101 588 1308 1269
Fragments arranged by size:
1308 1269 1101 972 588 537
PasI CC'CwG_GG
Cuts at: 0 1125 3717 4140 5775
Size: 1125 2592 423 1635
Fragments arranged by size:
2592 1635 1125 423
PflMI CCAn_nnn'nTGG
Cuts at: 0 405 1620 2577 2955 3042 4287 5775
Size: 405 1215 957 378 87 1245 1488
Fragments arranged by size:
1488 1245 1215 957 405 378 87
PfoI T'CCnGG_A
Cuts at: 0 2504 4043 4760 5096 5228 5525 5775
Size: 2504 1539 717 336 132 297 250
Fragments arranged by size:
2504 1539 717 336 297 250 132
PmlI CAC'GTG
Cuts at: 0 2034 5775
Size: 2034 3741
PpuMI rG'GwC_Cy
Cuts at: 0 1130 1944 2717 3039 4863 5775
Size: 1130 814 773 322 1824 912
Fragments arranged by size:
1824 1130 912 814 773 322
PshAI GACnn'nnGTC
Cuts at: 0 2594 4744 5775
Size: 2594 2150 1031
Fragments arranged by size:
2594 2150 1031
PsiI TTA'TAA
Cuts at: 0 5633 5775
Size: 5633 142
PspOMI G'GGCC_C
Cuts at: 0 285 1785 2970 3116 3383 5775
Size: 285 1500 1185 146 267 2392
Fragments arranged by size:
2392 1500 1185 285 267 146
PstI C_TGCA'G
Cuts at: 0 386 1352 1373 1865 2107 2645 3791 3838
Size: 386 966 21 492 242 538 1146 47
Cuts at: 3838 3929 3989 5435 5711 5775
Size: 91 60 1446 276 64
Fragments arranged by size:
1446 1146 966 538 492 386 276 242
91 64 60 47 21
PvuII CAG'CTG
Cuts at: 0 243 324 387 447 1326 2147 3738 3792
Size: 243 81 63 60 879 821 1591 54
Cuts at: 3792 4511 5436 5775
Size: 719 925 339
Fragments arranged by size:
1591 925 879 821 719 339 243 81
63 60 54
SacI G_AGCT'C
Cuts at: 0 3035 5775
Size: 3035 2740
SacII CC_GC'GG
Cuts at: 0 17 82 352 599 1053 1139 5775
Size: 17 65 270 247 454 86 4636
Fragments arranged by size:
4636 454 270 247 86 65 17
SalI G'TCGA_C
Cuts at: 0 4816 5775
Size: 4816 959
SanDI GG'GwC_CC
Cuts at: 0 1130 1944 2717 5775
Size: 1130 814 773 3058
Fragments arranged by size:
3058 1130 814 773
SapI GCTCTTCn'nnn_
Cuts at: 0 760 2446 4097 5775
Size: 760 1686 1651 1678
Fragments arranged by size:
1686 1678 1651 760
ScaI AGT'ACT
Cuts at: 0 4372 5215 5775
Size: 4372 843 560
Fragments arranged by size:
4372 843 560
SexAI A'CCwGG_T
Cuts at: 0 2426 5775
Size: 2426 3349
SfcI C'TryA_G
Cuts at: 0 382 1062 1348 1369 1861 2103 2641 3787
Size: 382 680 286 21 492 242 538 1146
Cuts at: 3787 3834 3925 3985 5431 5707 5763 5775
Size: 47 91 60 1446 276 56 12
Fragments arranged by size:
1446 1146 680 538 492 382 286 276
242 91 60 56 47 21 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 277 5775
Size: 277 5498
SfoI GGC'GCC
Cuts at: 0 135 252 355 454 630 810 978 1235
Size: 135 117 103 99 176 180 168 257
Cuts at: 1235 5775
Size: 4540
Fragments arranged by size:
4540 257 180 176 168 135 117 103
99
SgrAI Cr'CCGG_yG
Cuts at: 0 979 5775
Size: 979 4796
SmaI CCC'GGG
Cuts at: 0 40 1093 1406 2917 3381 3573 5775
Size: 40 1053 313 1511 464 192 2202
Fragments arranged by size:
2202 1511 1053 464 313 192 40
SmlI C'TyrA_G
Cuts at: 0 793 1483 4822 5775
Size: 793 690 3339 953
Fragments arranged by size:
3339 953 793 690
SphI G_CATG'C
Cuts at: 0 537 1509 5775
Size: 537 972 4266
Fragments arranged by size:
4266 972 537
SrfI GCCC'GGGC
Cuts at: 0 40 5775
Size: 40 5735
StuI AGG'CCT
Cuts at: 0 334 2224 4768 5401 5775
Size: 334 1890 2544 633 374
Fragments arranged by size:
2544 1890 633 374 334
StyI C'CwwG_G
Cuts at: 0 812 2549 2648 2966 2975 3003 3302 4010
Size: 812 1737 99 318 9 28 299 708
Cuts at: 4010 4721 5129 5474 5618 5775
Size: 711 408 345 144 157
Fragments arranged by size:
1737 812 711 708 408 345 318 299
157 144 99 28 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1068 1666 2388 3693 4184 5304 5775
Size: 1068 598 722 1305 491 1120 471
Fragments arranged by size:
1305 1120 1068 722 598 491 471
TatI w'GTAC_w
Cuts at: 0 4370 4505 5213 5775
Size: 4370 135 708 562
Fragments arranged by size:
4370 708 562 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1345 1377 1705 1737 2775 2807 2827 2859
Size: 1345 32 328 32 1038 32 20 32
Cuts at: 2859 5775
Size: 2916
Fragments arranged by size:
2916 1345 1038 328 32 32 32 32
20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 723 2529 3789 5616 5775
Size: 261 462 1806 1260 1827 159
Fragments arranged by size:
1827 1806 1260 462 261 159
XmaI C'CCGG_G
Cuts at: 0 38 1091 1404 2915 3379 3571 5775
Size: 38 1053 313 1511 464 192 2204
Fragments arranged by size:
2204 1511 1053 464 313 192 38
XmnI GAAnn'nnTTC
Cuts at: 0 5017 5599 5775
Size: 5017 582 176
Fragments arranged by size:
5017 582 176
ZraI GAC'GTC
Cuts at: 0 1610 4815 5775
Size: 1610 3205 960
Fragments arranged by size:
3205 1610 960
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AvaI BaeI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI
HaeII Hin4I HincII HindIII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PstI
PvuII SacI SacII SalI SanDI SapI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AhdI ApoI AscI AseI AsiSI
AvrII BamHI BclI BfrBI BmtI BplI BsaI BsiWI
BspEI BsrDI BstBI BstZ17I CspCI DraI DrdI EcoRI
EcoRV FseI HpaI KpnI MfeI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PspXI PsrI
PvuI RsrII SbfI SgfI SnaBI SpeI SspI SwaI
Tth111I XbaI XhoI