Restriction map for
pF1KA0621
(Linear) (Six-base) MAPSORT of: KIAA0621.seq Check: 6523 from: 1 to: 2166
KIAA0621.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 1022 1109 2166
Size: 1022 87 1057
Fragments arranged by size:
1057 1022 87
Acc65I G'GTAC_C
Cuts at: 0 900 2166
Size: 900 1266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 463 1152 1378 2166
Size: 146 317 689 226 788
Fragments arranged by size:
788 689 317 226 146
AfeI AGC'GCT
Cuts at: 0 14 2166
Size: 14 2152
AhdI GACnn_n'nnGTC
Cuts at: 0 129 788 915 2166
Size: 129 659 127 1251
Fragments arranged by size:
1251 659 129 127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 284 2166
Size: 284 1882
AlwNI CAG_nnn'CTG
Cuts at: 0 29 2166
Size: 29 2137
ApoI r'AATT_y
Cuts at: 0 100 153 196 202 447 1560 1651 2166
Size: 100 53 43 6 245 1113 91 515
Fragments arranged by size:
1113 515 245 100 91 53 43 6
AvaI C'yCGr_G
Cuts at: 0 16 1451 2166
Size: 16 1435 715
Fragments arranged by size:
1435 715 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 884 917 2166
Size: 884 33 1249
Fragments arranged by size:
1249 884 33
BanI G'GyrC_C
Cuts at: 0 900 2166
Size: 900 1266
BanII G_rGCy'C
Cuts at: 0 8 116 2166
Size: 8 108 2050
Fragments arranged by size:
2050 108 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 152 446 537 1366 2061 2166
Size: 152 294 91 829 695 105
Fragments arranged by size:
829 695 294 152 105 91
BbvCI CC'TCA_GC
Cuts at: 0 1609 2166
Size: 1609 557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1732 1766 2052 2086 2166
Size: 1732 34 286 34 80
Fragments arranged by size:
1732 286 80 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 296 540 802 2166
Size: 296 244 262 1364
Fragments arranged by size:
1364 296 262 244
BfrBI ATG'CAT
Cuts at: 0 1187 2166
Size: 1187 979
Bme1580I G_kGCm'C
Cuts at: 0 1715 2166
Size: 1715 451
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 79 2166
Size: 79 2087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1236 1456 1848 1923 2166
Size: 1236 220 392 75 243
Fragments arranged by size:
1236 392 243 220 75
Bpu10I CC'TnA_GC
Cuts at: 0 1609 2166
Size: 1609 557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 133 793 2166
Size: 49 84 660 1373
Fragments arranged by size:
1373 660 84 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 1461 2166
Size: 1461 705
BsaAI yAC'GTr
Cuts at: 0 819 2154 2166
Size: 819 1335 12
Fragments arranged by size:
1335 819 12
BsaWI w'CCGG_w
Cuts at: 0 1067 1997 2166
Size: 1067 930 169
Fragments arranged by size:
1067 930 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1921 1951 2166
Size: 1921 30 215
Fragments arranged by size:
1921 215 30
BseYI C'CCAG_C
Cuts at: 0 9 1736 2166
Size: 9 1727 430
Fragments arranged by size:
1727 430 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 841 948 1279 1726 2166
Size: 574 267 107 331 447 440
Fragments arranged by size:
574 447 440 331 267 107
BsiHKAI G_wGCw'C
Cuts at: 0 116 335 779 1356 2166
Size: 116 219 444 577 810
Fragments arranged by size:
810 577 444 219 116
BsmI GAATG_Cn'
Cuts at: 0 324 1379 2166
Size: 324 1055 787
Fragments arranged by size:
1055 787 324
BsmBI CGTCTCn'nnnn_
Cuts at: 0 52 2166
Size: 52 2114
Bsp1286I G_dGCh'C
Cuts at: 0 8 116 335 779 1356 1715 2166
Size: 8 108 219 444 577 359 451
Fragments arranged by size:
577 451 444 359 219 108 8
BspEI T'CCGG_A
Cuts at: 0 1997 2166
Size: 1997 169
BspHI T'CATG_A
Cuts at: 0 1391 1520 2166
Size: 1391 129 646
Fragments arranged by size:
1391 646 129
BsrGI T'GTAC_A
Cuts at: 0 865 2166
Size: 865 1301
BssHII G'CGCG_C
Cuts at: 0 143 2166
Size: 143 2023
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1266 1540 2166
Size: 1266 274 626
Fragments arranged by size:
1266 626 274
BstBI TT'CG_AA
Cuts at: 0 357 2166
Size: 357 1809
BstYI r'GATC_y
Cuts at: 0 1673 2166
Size: 1673 493
Bsu36I CC'TnA_GG
Cuts at: 0 280 1616 2166
Size: 280 1336 550
Fragments arranged by size:
1336 550 280
BtgI C'CryG_G
Cuts at: 0 636 800 896 1088 2166
Size: 636 164 96 192 1078
Fragments arranged by size:
1078 636 192 164 96
BtsI GCAGTG_nn'
Cuts at: 0 1770 2166
Size: 1770 396
DraI TTT'AAA
Cuts at: 0 201 2166
Size: 201 1965
DrdI GACnn_nn'nnGTC
Cuts at: 0 66 2166
Size: 66 2100
EaeI y'GGCC_r
Cuts at: 0 650 1095 2166
Size: 650 445 1071
Fragments arranged by size:
1071 650 445
EarI CTCTTCn'nnn_
Cuts at: 0 625 988 1055 1753 1813 2166
Size: 625 363 67 698 60 353
Fragments arranged by size:
698 625 363 353 67 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 987 2166
Size: 987 1179
EcoICRI GAG'CTC
Cuts at: 0 114 2166
Size: 114 2052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 463 1152 1236 1378 1456 1848 1923
Size: 146 317 689 84 142 78 392 75
Cuts at: 1923 2166
Size: 243
Fragments arranged by size:
689 392 317 243 146 142 84 78
75
EcoO109I rG'GnC_Cy
Cuts at: 0 1796 2166
Size: 1796 370
EcoRV GAT'ATC
Cuts at: 0 1317 2166
Size: 1317 849
FspI TGC'GCA
Cuts at: 0 1148 2166
Size: 1148 1018
HaeII r_GCGC'y
Cuts at: 0 16 2166
Size: 16 2150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 774 806 901 933 2166
Size: 115 32 627 32 95 32 1233
Fragments arranged by size:
1233 627 115 95 32 32 32
HincII GTy'rAC
Cuts at: 0 683 1248 1826 2166
Size: 683 565 578 340
Fragments arranged by size:
683 578 565 340
HindIII A'AGCT_T
Cuts at: 0 1049 2166
Size: 1049 1117
HpaI GTT'AAC
Cuts at: 0 683 2166
Size: 683 1483
KpnI G_GTAC'C
Cuts at: 0 904 2166
Size: 904 1262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1133 2087 2166
Size: 1037 96 954 79
Fragments arranged by size:
1037 954 96 79
MscI TGG'CCA
Cuts at: 0 652 2166
Size: 652 1514
MslI CAynn'nnrTG
Cuts at: 0 521 2166
Size: 521 1645
MspA1I CmG'CkG
Cuts at: 0 1515 1740 2166
Size: 1515 225 426
Fragments arranged by size:
1515 426 225
NcoI C'CATG_G
Cuts at: 0 636 800 896 1088 2166
Size: 636 164 96 192 1078
Fragments arranged by size:
1078 636 192 164 96
NsiI A_TGCA'T
Cuts at: 0 1189 2166
Size: 1189 977
PflMI CCAn_nnn'nTGG
Cuts at: 0 1197 2166
Size: 1197 969
PfoI T'CCnGG_A
Cuts at: 0 1380 2166
Size: 1380 786
PspXI vC'TCGA_Gb
Cuts at: 0 16 2166
Size: 16 2150
PstI C_TGCA'G
Cuts at: 0 1789 2166
Size: 1789 377
PvuII CAG'CTG
Cuts at: 0 1515 1740 2166
Size: 1515 225 426
Fragments arranged by size:
1515 426 225
SacI G_AGCT'C
Cuts at: 0 116 2166
Size: 116 2050
SapI GCTCTTCn'nnn_
Cuts at: 0 1753 1813 2166
Size: 1753 60 353
Fragments arranged by size:
1753 353 60
SexAI A'CCwGG_T
Cuts at: 0 503 2166
Size: 503 1663
SfcI C'TryA_G
Cuts at: 0 1785 2166
Size: 1785 381
SmlI C'TyrA_G
Cuts at: 0 16 64 148 772 2166
Size: 16 48 84 624 1394
Fragments arranged by size:
1394 624 84 48 16
SpeI A'CTAG_T
Cuts at: 0 1348 2166
Size: 1348 818
StuI AGG'CCT
Cuts at: 0 1614 2166
Size: 1614 552
StyI C'CwwG_G
Cuts at: 0 344 380 636 653 800 896 1088 1581
Size: 344 36 256 17 147 96 192 493
Cuts at: 1581 2166
Size: 585
Fragments arranged by size:
585 493 344 256 192 147 96 36
17
SwaI ATTT'AAAT
Cuts at: 0 201 2166
Size: 201 1965
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 840 2099 2166
Size: 840 1259 67
Fragments arranged by size:
1259 840 67
TatI w'GTAC_w
Cuts at: 0 865 2166
Size: 865 1301
Tth111I GACn'n_nGTC
Cuts at: 0 505 2166
Size: 505 1661
XhoI C'TCGA_G
Cuts at: 0 16 2166
Size: 16 2150
Enzymes that do cut:
AccI Acc65I AcuI AfeI AhdI AlfI AlwNI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BcgI BciVI
BfrBI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BsrGI BssHII BstAPI BstBI BstYI Bsu36I
BtgI BtsI DraI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MmeI MscI MslI MspA1I NcoI NsiI
PflMI PfoI PspXI PstI PvuII SacI SapI SexAI
SfcI SmlI SpeI StuI StyI SwaI TaqII TatI
Tth111I XhoI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BclI BglI BglII BlpI BmgBI BmrI BmtI BsaBI
BsaHI BseRI BsiEI BsiWI BspMI BsrBI BsrDI BsrFI
BssSI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII
EagI EcoNI EcoRI FalI FseI FspAI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI TstI XbaI XcmI XmaI
XmnI ZraI