Restriction map for
pF1KA0623
(Linear) (Six-base) MAPSORT of: KIAA0623.seq Check: 2501 from: 1 to: 3108
KIAA0623.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 2049 3108
Size: 2049 1059
Acc65I G'GTAC_C
Cuts at: 0 1444 3108
Size: 1444 1664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 739 1907 3108
Size: 172 567 1168 1201
Fragments arranged by size:
1201 1168 567 172
AflII C'TTAA_G
Cuts at: 0 663 3108
Size: 663 2445
AflIII A'CryG_T
Cuts at: 0 2222 3108
Size: 2222 886
AleI CACnn'nnGTG
Cuts at: 0 1094 2746 3108
Size: 1094 1652 362
Fragments arranged by size:
1652 1094 362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 359 2815 3108
Size: 359 2456 293
Fragments arranged by size:
2456 359 293
ApaI G_GGCC'C
Cuts at: 0 2258 3108
Size: 2258 850
ApaLI G'TGCA_C
Cuts at: 0 2559 3108
Size: 2559 549
ApoI r'AATT_y
Cuts at: 0 1138 2785 3108
Size: 1138 1647 323
Fragments arranged by size:
1647 1138 323
AseI AT'TA_AT
Cuts at: 0 122 3108
Size: 122 2986
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1297 1330 3108
Size: 1297 33 1778
Fragments arranged by size:
1778 1297 33
BamHI G'GATC_C
Cuts at: 0 526 3108
Size: 526 2582
BanI G'GyrC_C
Cuts at: 0 80 1065 1444 2130 3108
Size: 80 985 379 686 978
Fragments arranged by size:
985 978 686 379 80
BanII G_rGCy'C
Cuts at: 0 2258 2275 2414 2737 3108
Size: 2258 17 139 323 371
Fragments arranged by size:
2258 371 323 139 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 60 320 1403 3078 3108
Size: 60 260 1083 1675 30
Fragments arranged by size:
1675 1083 260 60 30
BbvCI CC'TCA_GC
Cuts at: 0 1688 3108
Size: 1688 1420
BglI GCCn_nnn'nGGC
Cuts at: 0 1331 1698 3108
Size: 1331 367 1410
Fragments arranged by size:
1410 1331 367
BglII A'GATC_T
Cuts at: 0 390 1294 1996 3108
Size: 390 904 702 1112
Fragments arranged by size:
1112 904 702 390
BlpI GC'TnA_GC
Cuts at: 0 2623 3108
Size: 2623 485
Bme1580I G_kGCm'C
Cuts at: 0 854 1654 1929 2258 2563 3108
Size: 854 800 275 329 305 545
Fragments arranged by size:
854 800 545 329 305 275
BmgBI CAC'GTC
Cuts at: 0 1126 3108
Size: 1126 1982
BmrI ACTGGGnnnn_n'
Cuts at: 0 835 1354 1415 1673 1688 2643 3108
Size: 835 519 61 258 15 955 465
Fragments arranged by size:
955 835 519 465 258 61 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1485 1511 2327 3108
Size: 1485 26 816 781
Fragments arranged by size:
1485 816 781 26
Bpu10I CC'TnA_GC
Cuts at: 0 578 1266 1454 1688 2552 3108
Size: 578 688 188 234 864 556
Fragments arranged by size:
864 688 578 556 234 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 832 1823 3108
Size: 641 191 991 1285
Fragments arranged by size:
1285 991 641 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 266 2189 3108
Size: 266 1923 919
Fragments arranged by size:
1923 919 266
BsaAI yAC'GTr
Cuts at: 0 2376 3108
Size: 2376 732
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1800 1830 3108
Size: 1800 30 1278
Fragments arranged by size:
1800 1278 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1506 2177 2458 3108
Size: 1506 671 281 650
Fragments arranged by size:
1506 671 650 281
BseYI C'CCAG_C
Cuts at: 0 1669 2257 2362 2398 3108
Size: 1669 588 105 36 710
Fragments arranged by size:
1669 710 588 105 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 3108
Size: 349 2759
BsiEI CG_ry'CG
Cuts at: 0 1345 3108
Size: 1345 1763
BsiHKAI G_wGCw'C
Cuts at: 0 2563 3108
Size: 2563 545
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2440 3108
Size: 2440 668
Bsp1286I G_dGCh'C
Cuts at: 0 854 1654 1929 2258 2275 2414 2563 2737
Size: 854 800 275 329 17 139 149 174
Cuts at: 2737 3108
Size: 371
Fragments arranged by size:
854 800 371 329 275 174 149 139
17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2131 3108
Size: 2131 977
BsrBI CCG'CTC
Cuts at: 0 2460 3108
Size: 2460 648
BsrDI GCAATG_nn'
Cuts at: 0 270 1929 3108
Size: 270 1659 1179
Fragments arranged by size:
1659 1179 270
BsrGI T'GTAC_A
Cuts at: 0 536 1278 2660 3108
Size: 536 742 1382 448
Fragments arranged by size:
1382 742 536 448
BssSI C'ACGA_G
Cuts at: 0 40 3108
Size: 40 3068
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2137 3108
Size: 2137 971
BstBI TT'CG_AA
Cuts at: 0 2232 3108
Size: 2232 876
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1329 1734 3108
Size: 1329 405 1374
Fragments arranged by size:
1374 1329 405
BstYI r'GATC_y
Cuts at: 0 36 390 526 1294 1996 2585 3108
Size: 36 354 136 768 702 589 523
Fragments arranged by size:
768 702 589 523 354 136 36
Bsu36I CC'TnA_GG
Cuts at: 0 548 1487 1518 3108
Size: 548 939 31 1590
Fragments arranged by size:
1590 939 548 31
BtgI C'CryG_G
Cuts at: 0 62 1328 1747 1915 3108
Size: 62 1266 419 168 1193
Fragments arranged by size:
1266 1193 419 168 62
BtsI GCAGTG_nn'
Cuts at: 0 1165 1307 1991 3108
Size: 1165 142 684 1117
Fragments arranged by size:
1165 1117 684 142
ClaI AT'CG_AT
Cuts at: 0 2829 3108
Size: 2829 279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2231 2266 3108
Size: 2231 35 842
Fragments arranged by size:
2231 842 35
DraI TTT'AAA
Cuts at: 0 1770 2075 3108
Size: 1770 305 1033
Fragments arranged by size:
1770 1033 305
DraIII CAC_nnn'GTG
Cuts at: 0 523 2197 2513 3108
Size: 523 1674 316 595
Fragments arranged by size:
1674 595 523 316
EaeI y'GGCC_r
Cuts at: 0 1342 2292 3108
Size: 1342 950 816
Fragments arranged by size:
1342 950 816
EagI C'GGCC_G
Cuts at: 0 1342 3108
Size: 1342 1766
EarI CTCTTCn'nnn_
Cuts at: 0 1041 2176 2340 3108
Size: 1041 1135 164 768
Fragments arranged by size:
1135 1041 768 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 739 1485 1511 1907 2327 3108
Size: 172 567 746 26 396 420 781
Fragments arranged by size:
781 746 567 420 396 172 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2138 2321 3108
Size: 2138 183 787
Fragments arranged by size:
2138 787 183
EcoO109I rG'GnC_Cy
Cuts at: 0 53 979 1739 2254 2309 3021 3108
Size: 53 926 760 515 55 712 87
Fragments arranged by size:
926 760 712 515 87 55 53
EcoRI G'AATT_C
Cuts at: 0 1138 3108
Size: 1138 1970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 603 3108
Size: 603 2505
FspI TGC'GCA
Cuts at: 0 2803 3108
Size: 2803 305
HaeII r_GCGC'y
Cuts at: 0 1586 3085 3108
Size: 1586 1499 23
Fragments arranged by size:
1586 1499 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1382 1414 1800 1832 3108
Size: 1382 32 386 32 1276
Fragments arranged by size:
1382 1276 386 32 32
HincII GTy'rAC
Cuts at: 0 2050 3108
Size: 2050 1058
KpnI G_GTAC'C
Cuts at: 0 1448 3108
Size: 1448 1660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 1340 1368 1874 3108
Size: 606 734 28 506 1234
Fragments arranged by size:
1234 734 606 506 28
MslI CAynn'nnrTG
Cuts at: 0 572 1094 1920 2489 2746 2797 3108
Size: 572 522 826 569 257 51 311
Fragments arranged by size:
826 572 569 522 311 257 51
MspA1I CmG'CkG
Cuts at: 0 1962 2622 2652 2679 2746 3108
Size: 1962 660 30 27 67 362
Fragments arranged by size:
1962 660 362 67 30 27
NcoI C'CATG_G
Cuts at: 0 1328 3108
Size: 1328 1780
NspI r_CATG'y
Cuts at: 0 2226 3108
Size: 2226 882
PciI A'CATG_T
Cuts at: 0 2222 3108
Size: 2222 886
PfoI T'CCnGG_A
Cuts at: 0 1501 2343 2588 2722 3108
Size: 1501 842 245 134 386
Fragments arranged by size:
1501 842 386 245 134
PpuMI rG'GwC_Cy
Cuts at: 0 979 1739 2309 3021 3108
Size: 979 760 570 712 87
Fragments arranged by size:
979 760 712 570 87
PspOMI G'GGCC_C
Cuts at: 0 2254 3108
Size: 2254 854
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2900 2932 3108
Size: 2900 32 176
Fragments arranged by size:
2900 176 32
PstI C_TGCA'G
Cuts at: 0 1302 1580 2142 2883 2928 3030 3108
Size: 1302 278 562 741 45 102 78
Fragments arranged by size:
1302 741 562 278 102 78 45
PvuII CAG'CTG
Cuts at: 0 2622 2652 2679 2746 3108
Size: 2622 30 27 67 362
Fragments arranged by size:
2622 362 67 30 27
SalI G'TCGA_C
Cuts at: 0 2048 3108
Size: 2048 1060
SanDI GG'GwC_CC
Cuts at: 0 2309 3108
Size: 2309 799
SapI GCTCTTCn'nnn_
Cuts at: 0 2340 3108
Size: 2340 768
SbfI CC_TGCA'GG
Cuts at: 0 2142 3108
Size: 2142 966
ScaI AGT'ACT
Cuts at: 0 1020 3108
Size: 1020 2088
SfcI C'TryA_G
Cuts at: 0 1298 1576 2138 2177 2879 2906 2924 3016
Size: 1298 278 562 39 702 27 18 92
Cuts at: 3016 3026 3108
Size: 10 82
Fragments arranged by size:
1298 702 562 278 92 82 39 27
18 10
SmlI C'TyrA_G
Cuts at: 0 656 663 811 1838 3108
Size: 656 7 148 1027 1270
Fragments arranged by size:
1270 1027 656 148 7
SpeI A'CTAG_T
Cuts at: 0 1021 3108
Size: 1021 2087
SspI AAT'ATT
Cuts at: 0 201 3108
Size: 201 2907
StuI AGG'CCT
Cuts at: 0 1419 3002 3108
Size: 1419 1583 106
Fragments arranged by size:
1583 1419 106
StyI C'CwwG_G
Cuts at: 0 1328 1710 1862 2159 3108
Size: 1328 382 152 297 949
Fragments arranged by size:
1328 949 382 297 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1 2968 3108
Size: 1 2967 140
Fragments arranged by size:
2967 140 1
TatI w'GTAC_w
Cuts at: 0 23 536 1018 1278 2660 3108
Size: 23 513 482 260 1382 448
Fragments arranged by size:
1382 513 482 448 260 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 294 326 1723 1755 3108
Size: 294 32 1397 32 1353
Fragments arranged by size:
1397 1353 294 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 622 1666 1731 3108
Size: 622 1044 65 1377
Fragments arranged by size:
1377 1044 622 65
XmnI GAAnn'nnTTC
Cuts at: 0 2830 2915 3108
Size: 2830 85 193
Fragments arranged by size:
2830 193 85
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AlfI ApaI
ApaLI ApoI AseI BaeI BamHI BanI BanII BbsI
BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI
ClaI CspCI DraI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KpnI MmeI MslI MspA1I NcoI NspI PciI PfoI
PpuMI PspOMI PsrI PstI PvuII SalI SanDI SapI
SbfI ScaI SfcI SmlI SpeI SspI StuI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AloI AlwNI
AscI AsiSI AvaI AvrII BbeI BcgI BciVI BclI
BfrBI BmtI BplI BsaBI BsaHI BsaWI BsiWI BsmI
BspEI BspHI BsrFI BssHII BstEII BstZ17I BtgZI DrdI
EciI EcoICRI EcoRV FseI FspAI HindIII HpaI KasI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PvuI RsrII SacI SacII
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI ZraI