Restriction map for
pF1KSDA0632
(Linear) (Six-base) MAPSORT of: KIAA0632.seq Check: 1116 from: 1 to: 2100
KIAA0632.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 1713 2100
Size: 1713 387
Acc65I G'GTAC_C
Cuts at: 0 1919 2100
Size: 1919 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 511 586 715 781 837 1045 1054
Size: 120 391 75 129 66 56 208 9
Cuts at: 1054 1312 2100
Size: 258 788
Fragments arranged by size:
788 391 258 208 129 120 75 66
56 9
AgeI A'CCGG_T
Cuts at: 0 1916 2100
Size: 1916 184
AleI CACnn'nnGTG
Cuts at: 0 1282 1576 2100
Size: 1282 294 524
Fragments arranged by size:
1282 524 294
AlwNI CAG_nnn'CTG
Cuts at: 0 489 711 1983 2100
Size: 489 222 1272 117
Fragments arranged by size:
1272 489 222 117
ApaI G_GGCC'C
Cuts at: 0 39 2100
Size: 39 2061
AvaI C'yCGr_G
Cuts at: 0 207 986 1333 2100
Size: 207 779 347 767
Fragments arranged by size:
779 767 347 207
AvrII C'CTAG_G
Cuts at: 0 999 2100
Size: 999 1101
BanI G'GyrC_C
Cuts at: 0 129 1691 1919 2100
Size: 129 1562 228 181
Fragments arranged by size:
1562 228 181 129
BanII G_rGCy'C
Cuts at: 0 39 202 377 617 734 1259 2100
Size: 39 163 175 240 117 525 841
Fragments arranged by size:
841 525 240 175 163 117 39
BbeI G_GCGC'C
Cuts at: 0 133 2100
Size: 133 1967
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 895 2100
Size: 248 647 1205
Fragments arranged by size:
1205 647 248
BciVI GTATCCnnnnn_n'
Cuts at: 0 1236 2100
Size: 1236 864
BclI T'GATC_A
Cuts at: 0 437 2100
Size: 437 1663
BglI GCCn_nnn'nGGC
Cuts at: 0 91 1415 2100
Size: 91 1324 685
Fragments arranged by size:
1324 685 91
Bme1580I G_kGCm'C
Cuts at: 0 39 833 1220 1776 2088 2100
Size: 39 794 387 556 312 12
Fragments arranged by size:
794 556 387 312 39 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 1063 1890 2100
Size: 315 748 827 210
Fragments arranged by size:
827 748 315 210
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 35 2100
Size: 35 2065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 895 1171 1178 1432 1627 1903 1963
Size: 631 264 276 7 254 195 276 60
Cuts at: 1963 2100
Size: 137
Fragments arranged by size:
631 276 276 264 254 195 137 60
7
Bpu10I CC'TnA_GC
Cuts at: 0 1423 2100
Size: 1423 677
BsaI GGTCTCn'nnnn_
Cuts at: 0 841 1362 1595 2100
Size: 841 521 233 505
Fragments arranged by size:
841 521 505 233
BsaAI yAC'GTr
Cuts at: 0 834 2100
Size: 834 1266
BsaHI Gr'CG_yC
Cuts at: 0 130 623 2100
Size: 130 493 1477
Fragments arranged by size:
1477 493 130
BsaWI w'CCGG_w
Cuts at: 0 369 899 1203 1916 2030 2100
Size: 369 530 304 713 114 70
Fragments arranged by size:
713 530 369 304 114 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 271 301 2100
Size: 205 30 36 30 1799
Fragments arranged by size:
1799 205 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 135 138 262 265 268 271 279 511
Size: 135 3 124 3 3 3 8 232
Cuts at: 511 859 1060 2100
Size: 348 201 1040
Fragments arranged by size:
1040 348 232 201 135 124 8 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 1258 1380 1596 2100
Size: 1258 122 216 504
Fragments arranged by size:
1258 504 216 122
BsiHKAI G_wGCw'C
Cuts at: 0 734 1442 1541 2100
Size: 734 708 99 559
Fragments arranged by size:
734 708 559 99
Bsp1286I G_dGCh'C
Cuts at: 0 39 202 377 617 734 833 1220 1259
Size: 39 163 175 240 117 99 387 39
Cuts at: 1259 1442 1541 1776 2088 2100
Size: 183 99 235 312 12
Fragments arranged by size:
387 312 240 235 183 175 163 117
99 99 39 39 12
BspEI T'CCGG_A
Cuts at: 0 1203 2030 2100
Size: 1203 827 70
Fragments arranged by size:
1203 827 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 977 1670 2100
Size: 341 636 693 430
Fragments arranged by size:
693 636 430 341
BsrFI r'CCGG_y
Cuts at: 0 1388 1629 1916 2059 2100
Size: 1388 241 287 143 41
Fragments arranged by size:
1388 287 241 143 41
BstAPI GCAn_nnn'nTGC
Cuts at: 0 711 2100
Size: 711 1389
BstEII G'GTnAC_C
Cuts at: 0 1371 1746 2100
Size: 1371 375 354
Fragments arranged by size:
1371 375 354
Bsu36I CC'TnA_GG
Cuts at: 0 787 2100
Size: 787 1313
BtgI C'CryG_G
Cuts at: 0 44 224 512 1304 2100
Size: 44 180 288 792 796
Fragments arranged by size:
796 792 288 180 44
BtsI GCAGTG_nn'
Cuts at: 0 430 1289 1984 2100
Size: 430 859 695 116
Fragments arranged by size:
859 695 430 116
EaeI y'GGCC_r
Cuts at: 0 118 165 227 1084 1478 1682 1787 2100
Size: 118 47 62 857 394 204 105 313
Fragments arranged by size:
857 394 313 204 118 105 62 47
EarI CTCTTCn'nnn_
Cuts at: 0 1582 2100
Size: 1582 518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1864 2100
Size: 1864 236
EcoICRI GAG'CTC
Cuts at: 0 732 2100
Size: 732 1368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 511 586 631 715 781 837 895
Size: 120 391 75 45 84 66 56 58
Cuts at: 895 1045 1054 1171 1178 1312 1432 1627 1903
Size: 150 9 117 7 134 120 195 276
Cuts at: 1903 1963 2100
Size: 60 137
Fragments arranged by size:
391 276 195 150 137 134 120 120
117 84 75 66 60 58 56 45
9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 166 439 568 2100
Size: 166 273 129 1532
Fragments arranged by size:
1532 273 166 129
EcoO109I rG'GnC_Cy
Cuts at: 0 35 269 606 1005 1146 1407 1463 1647
Size: 35 234 337 399 141 261 56 184
Cuts at: 1647 1851 2100
Size: 204 249
Fragments arranged by size:
399 337 261 249 234 204 184 141
56 35
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 591 912 2100
Size: 591 321 1188
Fragments arranged by size:
1188 591 321
FspI TGC'GCA
Cuts at: 0 780 2100
Size: 780 1320
HaeII r_GCGC'y
Cuts at: 0 133 692 1624 2100
Size: 133 559 932 476
Fragments arranged by size:
932 559 476 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 41 73 271 303 918 950 2100
Size: 41 32 198 32 615 32 1150
Fragments arranged by size:
1150 615 198 41 32 32 32
HincII GTy'rAC
Cuts at: 0 1320 2100
Size: 1320 780
KasI G'GCGC_C
Cuts at: 0 129 2100
Size: 129 1971
KpnI G_GTAC'C
Cuts at: 0 1923 2100
Size: 1923 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 2100
Size: 242 1858
MscI TGG'CCA
Cuts at: 0 1684 2100
Size: 1684 416
MslI CAynn'nnrTG
Cuts at: 0 1282 1576 1729 2100
Size: 1282 294 153 371
Fragments arranged by size:
1282 371 294 153
MspA1I CmG'CkG
Cuts at: 0 156 711 1083 1161 1384 1881 2100
Size: 156 555 372 78 223 497 219
Fragments arranged by size:
555 497 372 223 219 156 78
NarI GG'CG_CC
Cuts at: 0 130 2100
Size: 130 1970
NcoI C'CATG_G
Cuts at: 0 44 512 2100
Size: 44 468 1588
Fragments arranged by size:
1588 468 44
NspI r_CATG'y
Cuts at: 0 1141 2100
Size: 1141 959
PasI CC'CwG_GG
Cuts at: 0 1040 2043 2100
Size: 1040 1003 57
Fragments arranged by size:
1040 1003 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 383 2100
Size: 383 1717
PfoI T'CCnGG_A
Cuts at: 0 292 1495 1529 2100
Size: 292 1203 34 571
Fragments arranged by size:
1203 571 292 34
PmlI CAC'GTG
Cuts at: 0 834 2100
Size: 834 1266
PpuMI rG'GwC_Cy
Cuts at: 0 269 606 1005 1463 1851 2100
Size: 269 337 399 458 388 249
Fragments arranged by size:
458 399 388 337 269 249
PspOMI G'GGCC_C
Cuts at: 0 35 2100
Size: 35 2065
PstI C_TGCA'G
Cuts at: 0 716 1509 1667 2100
Size: 716 793 158 433
Fragments arranged by size:
793 716 433 158
PvuII CAG'CTG
Cuts at: 0 156 711 1161 1384 1881 2100
Size: 156 555 450 223 497 219
Fragments arranged by size:
555 497 450 223 219 156
SacI G_AGCT'C
Cuts at: 0 734 2100
Size: 734 1366
SanDI GG'GwC_CC
Cuts at: 0 269 1005 1851 2100
Size: 269 736 846 249
Fragments arranged by size:
846 736 269 249
SapI GCTCTTCn'nnn_
Cuts at: 0 1582 2100
Size: 1582 518
SexAI A'CCwGG_T
Cuts at: 0 1541 2100
Size: 1541 559
SfcI C'TryA_G
Cuts at: 0 78 682 712 943 1349 1505 1663 1714
Size: 78 604 30 231 406 156 158 51
Cuts at: 1714 1767 2100
Size: 53 333
Fragments arranged by size:
604 406 333 231 158 156 78 53
51 30
SfoI GGC'GCC
Cuts at: 0 131 2100
Size: 131 1969
SmaI CCC'GGG
Cuts at: 0 1335 2100
Size: 1335 765
StuI AGG'CCT
Cuts at: 0 574 2100
Size: 574 1526
StyI C'CwwG_G
Cuts at: 0 44 512 999 1087 1106 1616 1923 2088
Size: 44 468 487 88 19 510 307 165
Cuts at: 2088 2100
Size: 12
Fragments arranged by size:
510 487 468 307 165 88 44 19
12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 2100
Size: 205 32 1863
Fragments arranged by size:
1863 205 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1137 2100
Size: 1137 963
XmaI C'CCGG_G
Cuts at: 0 1333 2100
Size: 1333 767
XmnI GAAnn'nnTTC
Cuts at: 0 574 2100
Size: 574 1526
Enzymes that do cut:
AccI Acc65I AcuI AgeI AleI AlwNI ApaI AvaI
AvrII BanI BanII BbeI BbsI BciVI BclI BglI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI
BsrFI BstAPI BstEII Bsu36I BtgI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SexAI SfcI
SfoI SmaI StuI StyI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbvCI BcgI BfrBI BglII BlpI BmgBI BmtI BpuEI
BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI
EcoRV FseI FspAI HindIII HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
Tth111I XbaI XhoI ZraI