Restriction map for
pF1KA0633
(Linear) (Six-base) MAPSORT of: KIAA0633.seq Check: 7724 from: 1 to: 3783
KIAA0633.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1137 3496 3783
Size: 1137 2359 287
Fragments arranged by size:
2359 1137 287
AatII G_ACGT'C
Cuts at: 0 194 1820 3783
Size: 194 1626 1963
Fragments arranged by size:
1963 1626 194
AccI GT'mk_AC
Cuts at: 0 2884 3783
Size: 2884 899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 311 437 2291 3783
Size: 265 46 126 1854 1492
Fragments arranged by size:
1854 1492 265 126 46
AflII C'TTAA_G
Cuts at: 0 2101 3783
Size: 2101 1682
AflIII A'CryG_T
Cuts at: 0 665 3783
Size: 665 3118
AgeI A'CCGG_T
Cuts at: 0 2007 3783
Size: 2007 1776
AleI CACnn'nnGTG
Cuts at: 0 2383 3783
Size: 2383 1400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2474 3783
Size: 2474 1309
AlwNI CAG_nnn'CTG
Cuts at: 0 1124 1152 3028 3751 3783
Size: 1124 28 1876 723 32
Fragments arranged by size:
1876 1124 723 32 28
ApaI G_GGCC'C
Cuts at: 0 132 3783
Size: 132 3651
ApaLI G'TGCA_C
Cuts at: 0 97 3783
Size: 97 3686
ApoI r'AATT_y
Cuts at: 0 317 739 1998 3280 3783
Size: 317 422 1259 1282 503
Fragments arranged by size:
1282 1259 503 422 317
AseI AT'TA_AT
Cuts at: 0 1450 3783
Size: 1450 2333
AvaI C'yCGr_G
Cuts at: 0 133 646 3783
Size: 133 513 3137
Fragments arranged by size:
3137 513 133
AvrII C'CTAG_G
Cuts at: 0 2484 2848 3783
Size: 2484 364 935
Fragments arranged by size:
2484 935 364
BanI G'GyrC_C
Cuts at: 0 447 1737 1881 2049 2063 2535 2697 2782
Size: 447 1290 144 168 14 472 162 85
Cuts at: 2782 3484 3685 3768 3783
Size: 702 201 83 15
Fragments arranged by size:
1290 702 472 447 201 168 162 144
85 83 15 14
BanII G_rGCy'C
Cuts at: 0 132 517 647 662 906 1143 1366 2359
Size: 132 385 130 15 244 237 223 993
Cuts at: 2359 2634 2844 3035 3530 3568 3783
Size: 275 210 191 495 38 215
Fragments arranged by size:
993 495 385 275 244 237 223 215
210 191 132 130 38 15
BbeI G_GCGC'C
Cuts at: 0 1741 1885 2701 3783
Size: 1741 144 816 1082
Fragments arranged by size:
1741 1082 816 144
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 1316 1448 1499 1517 2249 2933 3589
Size: 317 999 132 51 18 732 684 656
Cuts at: 3589 3593 3783
Size: 4 190
Fragments arranged by size:
999 732 684 656 317 190 132 51
18 4
BbvCI CC'TCA_GC
Cuts at: 0 172 3691 3783
Size: 172 3519 92
Fragments arranged by size:
3519 172 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1591 1625 3433 3467 3783
Size: 1591 34 1808 34 316
Fragments arranged by size:
1808 1591 316 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 22 213 3783
Size: 22 191 3570
Fragments arranged by size:
3570 191 22
BlpI GC'TnA_GC
Cuts at: 0 1751 3021 3783
Size: 1751 1270 762
Fragments arranged by size:
1751 1270 762
Bme1580I G_kGCm'C
Cuts at: 0 101 132 2390 2787 3479 3688 3773 3783
Size: 101 31 2258 397 692 209 85 10
Fragments arranged by size:
2258 692 397 209 101 85 31 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 538 1612 2781 3203 3783
Size: 538 1074 1169 422 580
Fragments arranged by size:
1169 1074 580 538 422
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3036 3783
Size: 3036 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 514 640 899 1478 2331 3140 3514
Size: 238 276 126 259 579 853 809 374
Cuts at: 3514 3654 3783
Size: 140 129
Fragments arranged by size:
853 809 579 374 276 259 238 140
129 126
Bpu10I CC'TnA_GC
Cuts at: 0 172 3560 3691 3783
Size: 172 3388 131 92
Fragments arranged by size:
3388 172 131 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1964 2554 3783
Size: 1964 590 1229
Fragments arranged by size:
1964 1229 590
BsaI GGTCTCn'nnnn_
Cuts at: 0 1162 3294 3783
Size: 1162 2132 489
Fragments arranged by size:
2132 1162 489
BsaAI yAC'GTr
Cuts at: 0 582 2529 3783
Size: 582 1947 1254
Fragments arranged by size:
1947 1254 582
BsaBI GATnn'nnATC
Cuts at: 0 1865 3783
Size: 1865 1918
BsaHI Gr'CG_yC
Cuts at: 0 191 1738 1817 1882 2698 3728 3783
Size: 191 1547 79 65 816 1030 55
Fragments arranged by size:
1547 1030 816 191 79 65 55
BsaWI w'CCGG_w
Cuts at: 0 2007 2182 3783
Size: 2007 175 1601
Fragments arranged by size:
2007 1601 175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1159 1189 1519 1549 2902 2932 3783
Size: 1159 30 330 30 1353 30 851
Fragments arranged by size:
1353 1159 851 330 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 240 580 628 899 902 1087 1180 2795
Size: 240 340 48 271 3 185 93 1615
Cuts at: 2795 3538 3783
Size: 743 245
Fragments arranged by size:
1615 743 340 271 245 240 185 93
48 3
BseYI C'CCAG_C
Cuts at: 0 834 852 1690 2205 2447 3256 3783
Size: 834 18 838 515 242 809 527
Fragments arranged by size:
838 834 809 527 515 242 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1783 2696 3310 3437 3638 3783
Size: 1783 913 614 127 201 145
Fragments arranged by size:
1783 913 614 201 145 127
BsiEI CG_ry'CG
Cuts at: 0 38 1211 3783
Size: 38 1173 2572
Fragments arranged by size:
2572 1173 38
BsiHKAI G_wGCw'C
Cuts at: 0 63 101 236 581 647 662 1090 1757
Size: 63 38 135 345 66 15 428 667
Cuts at: 1757 2359 3530 3783
Size: 602 1171 253
Fragments arranged by size:
1171 667 602 428 345 253 135 66
63 38 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1254 3783
Size: 1254 2529
Bsp1286I G_dGCh'C
Cuts at: 0 63 101 132 236 517 581 647 662
Size: 63 38 31 104 281 64 66 15
Cuts at: 662 906 1090 1143 1366 1757 2359 2390 2634
Size: 244 184 53 223 391 602 31 244
Cuts at: 2634 2787 2844 3035 3479 3530 3568 3688 3773
Size: 153 57 191 444 51 38 120 85
Cuts at: 3773 3783
Size: 10
Fragments arranged by size:
602 444 391 281 244 244 223 191
184 153 120 104 85 66 64 63
57 53 51 38 38 31 31 15
10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 491 1137 1800 2907 3496 3783
Size: 491 646 663 1107 589 287
Fragments arranged by size:
1107 663 646 589 491 287
BsrBI CCG'CTC
Cuts at: 0 3737 3783
Size: 3737 46
BsrDI GCAATG_nn'
Cuts at: 0 602 717 2612 3783
Size: 602 115 1895 1171
Fragments arranged by size:
1895 1171 602 115
BsrFI r'CCGG_y
Cuts at: 0 2007 2233 3783
Size: 2007 226 1550
Fragments arranged by size:
2007 1550 226
BssHII G'CGCG_C
Cuts at: 0 12 3123 3783
Size: 12 3111 660
Fragments arranged by size:
3111 660 12
BssSI C'ACGA_G
Cuts at: 0 56 3778 3783
Size: 56 3722 5
Fragments arranged by size:
3722 56 5
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1124 3514 3556 3783
Size: 1124 2390 42 227
Fragments arranged by size:
2390 1124 227 42
BstBI TT'CG_AA
Cuts at: 0 1906 3783
Size: 1906 1877
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 2060 2520 3783
Size: 186 1874 460 1263
Fragments arranged by size:
1874 1263 460 186
Bsu36I CC'TnA_GG
Cuts at: 0 443 518 592 3496 3783
Size: 443 75 74 2904 287
Fragments arranged by size:
2904 443 287 75 74
BtgI C'CryG_G
Cuts at: 0 185 197 1554 2513 2817 3135 3471 3783
Size: 185 12 1357 959 304 318 336 312
Fragments arranged by size:
1357 959 336 318 312 304 185 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 265 2166 3783
Size: 265 1901 1617
Fragments arranged by size:
1901 1617 265
BtsI GCAGTG_nn'
Cuts at: 0 1122 1323 1569 2840 3454 3783
Size: 1122 201 246 1271 614 329
Fragments arranged by size:
1271 1122 614 329 246 201
EaeI y'GGCC_r
Cuts at: 0 23 3250 3783
Size: 23 3227 533
Fragments arranged by size:
3227 533 23
EarI CTCTTCn'nnn_
Cuts at: 0 217 413 675 1078 1354 3783
Size: 217 196 262 403 276 2429
Fragments arranged by size:
2429 403 276 262 217 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1177 3783
Size: 1177 2606
EcoICRI GAG'CTC
Cuts at: 0 645 660 2357 3528 3783
Size: 645 15 1697 1171 255
Fragments arranged by size:
1697 1171 645 255 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 265 311 437 514 640 899 1478
Size: 238 27 46 126 77 126 259 579
Cuts at: 1478 2291 2331 3140 3514 3654 3783
Size: 813 40 809 374 140 129
Fragments arranged by size:
813 809 579 374 259 238 140 129
126 126 77 46 40 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 914 2488 3096 3158 3499 3783
Size: 914 1574 608 62 341 284
Fragments arranged by size:
1574 914 608 341 284 62
EcoO109I rG'GnC_Cy
Cuts at: 0 128 129 374 1326 1351 2368 3783
Size: 128 1 245 952 25 1017 1415
Fragments arranged by size:
1415 1017 952 245 128 25 1
EcoRI G'AATT_C
Cuts at: 0 1998 3783
Size: 1998 1785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1383 3783
Size: 1383 2400
HaeII r_GCGC'y
Cuts at: 0 172 900 1006 1741 1885 2701 3783
Size: 172 728 106 735 144 816 1082
Fragments arranged by size:
1082 816 735 728 172 144 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 39 71 931 963 1159 1191 1234 1266
Size: 39 32 860 32 196 32 43 32
Cuts at: 1266 1519 1551 2388 2420 2728 2760 3783
Size: 253 32 837 32 308 32 1023
Fragments arranged by size:
1023 860 837 308 253 196 43 39
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1737 1881 2697 3783
Size: 1737 144 816 1086
Fragments arranged by size:
1737 1086 816 144
MluI A'CGCG_T
Cuts at: 0 665 3783
Size: 665 3118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1178 1589 1800 3783
Size: 863 315 411 211 1983
Fragments arranged by size:
1983 863 411 315 211
MscI TGG'CCA
Cuts at: 0 3252 3783
Size: 3252 531
MslI CAynn'nnrTG
Cuts at: 0 2383 2518 2648 2669 2769 3783
Size: 2383 135 130 21 100 1014
Fragments arranged by size:
2383 1014 135 130 100 21
MspA1I CmG'CkG
Cuts at: 0 1187 3473 3482 3783
Size: 1187 2286 9 301
Fragments arranged by size:
2286 1187 301 9
NarI GG'CG_CC
Cuts at: 0 1738 1882 2698 3783
Size: 1738 144 816 1085
Fragments arranged by size:
1738 1085 816 144
NcoI C'CATG_G
Cuts at: 0 185 1554 2513 3783
Size: 185 1369 959 1270
Fragments arranged by size:
1369 1270 959 185
NspI r_CATG'y
Cuts at: 0 97 3783
Size: 97 3686
PasI CC'CwG_GG
Cuts at: 0 1107 1886 2083 3783
Size: 1107 779 197 1700
Fragments arranged by size:
1700 1107 779 197
PflMI CCAn_nnn'nTGG
Cuts at: 0 73 2601 3783
Size: 73 2528 1182
Fragments arranged by size:
2528 1182 73
PfoI T'CCnGG_A
Cuts at: 0 72 3156 3783
Size: 72 3084 627
Fragments arranged by size:
3084 627 72
PmlI CAC'GTG
Cuts at: 0 582 2529 3783
Size: 582 1947 1254
Fragments arranged by size:
1947 1254 582
PpuMI rG'GwC_Cy
Cuts at: 0 374 1326 1351 2368 3783
Size: 374 952 25 1017 1415
Fragments arranged by size:
1415 1017 952 374 25
PshAI GACnn'nnGTC
Cuts at: 0 1349 3593 3783
Size: 1349 2244 190
Fragments arranged by size:
2244 1349 190
PspOMI G'GGCC_C
Cuts at: 0 128 3783
Size: 128 3655
PstI C_TGCA'G
Cuts at: 0 1129 1318 3097 3493 3783
Size: 1129 189 1779 396 290
Fragments arranged by size:
1779 1129 396 290 189
PvuII CAG'CTG
Cuts at: 0 1187 3783
Size: 1187 2596
SacI G_AGCT'C
Cuts at: 0 647 662 2359 3530 3783
Size: 647 15 1697 1171 253
Fragments arranged by size:
1697 1171 647 253 15
SacII CC_GC'GG
Cuts at: 0 3474 3783
Size: 3474 309
SanDI GG'GwC_CC
Cuts at: 0 374 1351 2368 3783
Size: 374 977 1017 1415
Fragments arranged by size:
1415 1017 977 374
SapI GCTCTTCn'nnn_
Cuts at: 0 1078 1354 3783
Size: 1078 276 2429
Fragments arranged by size:
2429 1078 276
SfcI C'TryA_G
Cuts at: 0 1125 1314 2094 3093 3221 3489 3783
Size: 1125 189 780 999 128 268 294
Fragments arranged by size:
1125 999 780 294 268 189 128
SfoI GGC'GCC
Cuts at: 0 1739 1883 2699 3783
Size: 1739 144 816 1084
Fragments arranged by size:
1739 1084 816 144
SmlI C'TyrA_G
Cuts at: 0 1979 2101 2569 3783
Size: 1979 122 468 1214
Fragments arranged by size:
1979 1214 468 122
SspI AAT'ATT
Cuts at: 0 537 3783
Size: 537 3246
StuI AGG'CCT
Cuts at: 0 3091 3783
Size: 3091 692
StyI C'CwwG_G
Cuts at: 0 185 1554 2045 2484 2513 2848 3591 3658
Size: 185 1369 491 439 29 335 743 67
Cuts at: 3658 3783
Size: 125
Fragments arranged by size:
1369 743 491 439 335 185 125 67
29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 1225 3270 3783
Size: 311 914 2045 513
Fragments arranged by size:
2045 914 513 311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1792 1820 1824 1852 2766 2798 2902 2934
Size: 1792 28 4 28 914 32 104 32
Cuts at: 2934 3783
Size: 849
Fragments arranged by size:
1792 914 849 104 32 32 28 28
4
Tth111I GACn'n_nGTC
Cuts at: 0 1173 1246 3783
Size: 1173 73 2537
Fragments arranged by size:
2537 1173 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1330 2517 2598 3783
Size: 1330 1187 81 1185
Fragments arranged by size:
1330 1187 1185 81
XmnI GAAnn'nnTTC
Cuts at: 0 410 3280 3783
Size: 410 2870 503
Fragments arranged by size:
2870 503 410
ZraI GAC'GTC
Cuts at: 0 192 1818 3783
Size: 192 1626 1965
Fragments arranged by size:
1965 1626 192
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI Bsu36I BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I KasI MluI MmeI MscI MslI
MspA1I NarI NcoI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI
SapI SfcI SfoI SmlI SspI StuI StyI TaqII
TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AhdI AloI AscI AsiSI BaeI
BamHI BciVI BclI BfrBI BglII BmgBI BmtI BsiWI
BsmI BspEI BspHI BsrGI BstEII BstYI BstZ17I ClaI
CspCI DraI DraIII DrdI EagI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SalI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TatI XbaI XhoI XmaI