Restriction map for
pF1KSDA0638
(Linear) (Six-base) MAPSORT of: KIAA0638.seq Check: 8898 from: 1 to: 4131
KIAA0638.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3290 3950 4131
Size: 3290 660 181
Fragments arranged by size:
3290 660 181
AccI GT'mk_AC
Cuts at: 0 2762 4131
Size: 2762 1369
Acc65I G'GTAC_C
Cuts at: 0 852 2777 4131
Size: 852 1925 1354
Fragments arranged by size:
1925 1354 852
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1584 3184 3724 3913 4131
Size: 428 1156 1600 540 189 218
Fragments arranged by size:
1600 1156 540 428 218 189
AfeI AGC'GCT
Cuts at: 0 2041 3021 3826 4131
Size: 2041 980 805 305
Fragments arranged by size:
2041 980 805 305
AflII C'TTAA_G
Cuts at: 0 1377 4131
Size: 1377 2754
AflIII A'CryG_T
Cuts at: 0 3407 4131
Size: 3407 724
AhdI GACnn_n'nnGTC
Cuts at: 0 3408 4131
Size: 3408 723
AleI CACnn'nnGTG
Cuts at: 0 3982 4131
Size: 3982 149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2123 4131
Size: 2123 2008
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2410 2442 4131
Size: 2410 32 1689
Fragments arranged by size:
2410 1689 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1012 1105 1165 1839 2169 2220 2487 2637
Size: 1012 93 60 674 330 51 267 150
Cuts at: 2637 2787 3537 3609 4105 4131
Size: 150 750 72 496 26
Fragments arranged by size:
1012 750 674 496 330 267 150 150
93 72 60 51 26
ApaI G_GGCC'C
Cuts at: 0 232 461 3238 4131
Size: 232 229 2777 893
Fragments arranged by size:
2777 893 232 229
AvaI C'yCGr_G
Cuts at: 0 2234 2609 3240 4131
Size: 2234 375 631 891
Fragments arranged by size:
2234 891 631 375
BanI G'GyrC_C
Cuts at: 0 274 718 852 1581 1704 2777 4131
Size: 274 444 134 729 123 1073 1354
Fragments arranged by size:
1354 1073 729 444 274 134 123
BanII G_rGCy'C
Cuts at: 0 232 323 453 461 574 736 1048 1404
Size: 232 91 130 8 113 162 312 356
Cuts at: 1404 1555 1618 1636 2163 2580 3238 3349 3997
Size: 151 63 18 527 417 658 111 648
Cuts at: 3997 4131
Size: 134
Fragments arranged by size:
658 648 527 417 356 312 232 162
151 134 130 113 111 91 63 18
8
BbeI G_GCGC'C
Cuts at: 0 722 4131
Size: 722 3409
BbsI GAAGACnn'nnnn_
Cuts at: 0 2719 3712 3828 4131
Size: 2719 993 116 303
Fragments arranged by size:
2719 993 303 116
BbvCI CC'TCA_GC
Cuts at: 0 3182 4106 4131
Size: 3182 924 25
Fragments arranged by size:
3182 924 25
BglI GCCn_nnn'nGGC
Cuts at: 0 342 1346 1495 2239 3242 3291 4131
Size: 342 1004 149 744 1003 49 840
Fragments arranged by size:
1004 1003 840 744 342 149 49
BlpI GC'TnA_GC
Cuts at: 0 937 1612 2504 2980 3158 3760 4131
Size: 937 675 892 476 178 602 371
Fragments arranged by size:
937 892 675 602 476 371 178
Bme1580I G_kGCm'C
Cuts at: 0 232 277 461 845 1533 2177 2259 2292
Size: 232 45 184 384 688 644 82 33
Cuts at: 2292 3238 3597 4131
Size: 946 359 534
Fragments arranged by size:
946 688 644 534 384 359 232 184
82 45 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 809 1576 1652 4131
Size: 809 767 76 2479
Fragments arranged by size:
2479 809 767 76
BmtI G_CTAG'C
Cuts at: 0 2143 4131
Size: 2143 1988
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 768 1530 4131
Size: 768 762 2601
Fragments arranged by size:
2601 768 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 227 647 720 740 852 1142 1177
Size: 196 31 420 73 20 112 290 35
Cuts at: 1177 1211 1671 1789 1861 2110 2413 3361 3559
Size: 34 460 118 72 249 303 948 198
Cuts at: 3559 4131
Size: 572
Fragments arranged by size:
948 572 460 420 303 290 249 198
196 118 112 73 72 35 34 31
20
Bpu10I CC'TnA_GC
Cuts at: 0 2162 2579 3182 4106 4131
Size: 2162 417 603 924 25
Fragments arranged by size:
2162 924 603 417 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2044 2173 3832 4131
Size: 2044 129 1659 299
Fragments arranged by size:
2044 1659 299 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 1837 3082 3355 4131
Size: 1837 1245 273 776
Fragments arranged by size:
1837 1245 776 273
BsaAI yAC'GTr
Cuts at: 0 2178 3753 4131
Size: 2178 1575 378
Fragments arranged by size:
2178 1575 378
BsaHI Gr'CG_yC
Cuts at: 0 719 4131
Size: 719 3412
BsaWI w'CCGG_w
Cuts at: 0 2492 3683 4131
Size: 2492 1191 448
Fragments arranged by size:
2492 1191 448
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 634 2011 2080 2473 2476 2937 2940 2955
Size: 634 1377 69 393 3 461 3 15
Cuts at: 2955 2958 2961 3322 3460 3556 3559 4131
Size: 3 3 361 138 96 3 572
Fragments arranged by size:
1377 634 572 461 393 361 138 96
69 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 967 1053 1098 1207 1481 1524 1617 1897
Size: 967 86 45 109 274 43 93 280
Cuts at: 1897 1909 2090 2169 2563 2799 2975 3228 4002
Size: 12 181 79 394 236 176 253 774
Cuts at: 4002 4131
Size: 129
Fragments arranged by size:
967 774 394 280 274 253 236 181
176 129 109 93 86 79 45 43
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 291 2091 2608 3733 3971 4131
Size: 76 215 1800 517 1125 238 160
Fragments arranged by size:
1800 1125 517 238 215 160 76
BsiEI CG_ry'CG
Cuts at: 0 3843 4131
Size: 3843 288
BsiHKAI G_wGCw'C
Cuts at: 0 251 574 918 1113 1404 2089 2102 3320
Size: 251 323 344 195 291 685 13 1218
Cuts at: 3320 3349 3761 4131
Size: 29 412 370
Fragments arranged by size:
1218 685 412 370 344 323 291 251
195 29 13
BsmI GAATG_Cn'
Cuts at: 0 1130 2625 4131
Size: 1130 1495 1506
Fragments arranged by size:
1506 1495 1130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3877 4131
Size: 3877 254
Bsp1286I G_dGCh'C
Cuts at: 0 232 251 277 323 453 461 574 736
Size: 232 19 26 46 130 8 113 162
Cuts at: 736 845 918 1048 1113 1404 1533 1555 1618
Size: 109 73 130 65 291 129 22 63
Cuts at: 1618 1636 2089 2102 2163 2177 2259 2292 2580
Size: 18 453 13 61 14 82 33 288
Cuts at: 2580 3238 3320 3349 3597 3761 3997 4131
Size: 658 82 29 248 164 236 134
Fragments arranged by size:
658 453 291 288 248 236 232 164
162 134 130 130 129 113 109 82
82 73 65 63 61 46 33 29
26 22 19 18 14 13 8
BspEI T'CCGG_A
Cuts at: 0 2492 3683 4131
Size: 2492 1191 448
Fragments arranged by size:
2492 1191 448
BspHI T'CATG_A
Cuts at: 0 4124 4131
Size: 4124 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 596 701 864 2865 3290 3555 3683
Size: 275 321 105 163 2001 425 265 128
Cuts at: 3683 3751 3950 4131
Size: 68 199 181
Fragments arranged by size:
2001 425 321 275 265 199 181 163
128 105 68
BsrBI CCG'CTC
Cuts at: 0 1239 1722 1797 2157 2253 2286 2442 2885
Size: 1239 483 75 360 96 33 156 443
Cuts at: 2885 3510 3549 4131
Size: 625 39 582
Fragments arranged by size:
1239 625 582 483 443 360 156 96
75 39 33
BsrDI GCAATG_nn'
Cuts at: 0 306 4131
Size: 306 3825
BsrFI r'CCGG_y
Cuts at: 0 1340 1778 2476 3488 3842 4034 4131
Size: 1340 438 698 1012 354 192 97
Fragments arranged by size:
1340 1012 698 438 354 192 97
BssSI C'ACGA_G
Cuts at: 0 1961 4131
Size: 1961 2170
BstAPI GCAn_nnn'nTGC
Cuts at: 0 305 365 2220 3756 3773 4131
Size: 305 60 1855 1536 17 358
Fragments arranged by size:
1855 1536 358 305 60 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 4131
Size: 729 3402
BstYI r'GATC_y
Cuts at: 0 1434 2570 4131
Size: 1434 1136 1561
Fragments arranged by size:
1561 1434 1136
Bsu36I CC'TnA_GG
Cuts at: 0 1892 4131
Size: 1892 2239
BtgI C'CryG_G
Cuts at: 0 50 1491 3729 3777 4131
Size: 50 1441 2238 48 354
Fragments arranged by size:
2238 1441 354 50 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2505 4131
Size: 2505 1626
BtsI GCAGTG_nn'
Cuts at: 0 144 249 795 1239 2257 2290 2321 2779
Size: 144 105 546 444 1018 33 31 458
Cuts at: 2779 3073 3954 4131
Size: 294 881 177
Fragments arranged by size:
1018 881 546 458 444 294 177 144
105 33 31
DraIII CAC_nnn'GTG
Cuts at: 0 126 817 1570 3756 4131
Size: 126 691 753 2186 375
Fragments arranged by size:
2186 753 691 375 126
EaeI y'GGCC_r
Cuts at: 0 1076 2474 2478 2543 2792 4131
Size: 1076 1398 4 65 249 1339
Fragments arranged by size:
1398 1339 1076 249 65 4
EarI CTCTTCn'nnn_
Cuts at: 0 780 2147 2838 2964 3131 3955 4131
Size: 780 1367 691 126 167 824 176
Fragments arranged by size:
1367 824 780 691 176 167 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1036 2001 3567 4131
Size: 1036 965 1566 564
Fragments arranged by size:
1566 1036 965 564
EcoICRI GAG'CTC
Cuts at: 0 572 1402 3347 4131
Size: 572 830 1945 784
Fragments arranged by size:
1945 830 784 572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 227 428 647 720 740 852 1142
Size: 196 31 201 219 73 20 112 290
Cuts at: 1142 1177 1211 1584 1671 1789 1861 2110 2413
Size: 35 34 373 87 118 72 249 303
Cuts at: 2413 3184 3361 3559 3724 3913 4131
Size: 771 177 198 165 189 218
Fragments arranged by size:
771 373 303 290 249 219 218 201
198 196 189 177 165 118 112 87
73 72 35 34 31 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3541 4131
Size: 3541 590
EcoO109I rG'GnC_Cy
Cuts at: 0 28 64 228 458 535 585 832 1347
Size: 28 36 164 230 77 50 247 515
Cuts at: 1347 1888 1955 1976 2388 3235 3534 4131
Size: 541 67 21 412 847 299 597
Fragments arranged by size:
847 597 541 515 412 299 247 230
164 77 67 50 36 28 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1614 3711 4131
Size: 1614 2097 420
Fragments arranged by size:
2097 1614 420
FseI GG_CCGG'CC
Cuts at: 0 2480 4131
Size: 2480 1651
FspI TGC'GCA
Cuts at: 0 3946 4131
Size: 3946 185
HaeII r_GCGC'y
Cuts at: 0 690 722 1482 1791 1839 2043 2541 3023
Size: 690 32 760 309 48 204 498 482
Cuts at: 3023 3099 3828 4131
Size: 76 729 303
Fragments arranged by size:
760 729 690 498 482 309 303 204
76 48 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2562 2594 3394 3426 4131
Size: 2562 32 800 32 705
Fragments arranged by size:
2562 800 705 32 32
HincII GTy'rAC
Cuts at: 0 1120 4131
Size: 1120 3011
HindIII A'AGCT_T
Cuts at: 0 2858 4131
Size: 2858 1273
KasI G'GCGC_C
Cuts at: 0 718 4131
Size: 718 3413
KpnI G_GTAC'C
Cuts at: 0 856 2781 4131
Size: 856 1925 1350
Fragments arranged by size:
1925 1350 856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2417 4131
Size: 2417 1714
MscI TGG'CCA
Cuts at: 0 1078 2545 4131
Size: 1078 1467 1586
Fragments arranged by size:
1586 1467 1078
MslI CAynn'nnrTG
Cuts at: 0 727 2105 3323 3982 4131
Size: 727 1378 1218 659 149
Fragments arranged by size:
1378 1218 727 659 149
MspA1I CmG'CkG
Cuts at: 0 174 849 1065 1413 1691 2220 2325 2634
Size: 174 675 216 348 278 529 105 309
Cuts at: 2634 2815 2871 2933 3144 3181 3232 3609 4131
Size: 181 56 62 211 37 51 377 522
Fragments arranged by size:
675 529 522 377 348 309 278 216
211 181 174 105 62 56 51 37
NaeI GCC'GGC
Cuts at: 0 1342 2478 4131
Size: 1342 1136 1653
Fragments arranged by size:
1653 1342 1136
NarI GG'CG_CC
Cuts at: 0 719 4131
Size: 719 3412
NcoI C'CATG_G
Cuts at: 0 50 1491 3729 4131
Size: 50 1441 2238 402
Fragments arranged by size:
2238 1441 402 50
NgoMIV G'CCGG_C
Cuts at: 0 1340 2476 4131
Size: 1340 1136 1655
Fragments arranged by size:
1655 1340 1136
NheI G'CTAG_C
Cuts at: 0 2139 4131
Size: 2139 1992
NspI r_CATG'y
Cuts at: 0 363 3411 4131
Size: 363 3048 720
Fragments arranged by size:
3048 720 363
PasI CC'CwG_GG
Cuts at: 0 454 2994 4131
Size: 454 2540 1137
Fragments arranged by size:
2540 1137 454
PciI A'CATG_T
Cuts at: 0 3407 4131
Size: 3407 724
PflMI CCAn_nnn'nTGG
Cuts at: 0 51 1105 1272 1839 2436 4131
Size: 51 1054 167 567 597 1695
Fragments arranged by size:
1695 1054 597 567 167 51
PfoI T'CCnGG_A
Cuts at: 0 1193 1766 1802 2549 4131
Size: 1193 573 36 747 1582
Fragments arranged by size:
1582 1193 747 573 36
PmlI CAC'GTG
Cuts at: 0 2178 3753 4131
Size: 2178 1575 378
Fragments arranged by size:
2178 1575 378
PpuMI rG'GwC_Cy
Cuts at: 0 64 535 585 832 1888 4131
Size: 64 471 50 247 1056 2243
Fragments arranged by size:
2243 1056 471 247 64 50
PspOMI G'GGCC_C
Cuts at: 0 228 457 3234 4131
Size: 228 229 2777 897
Fragments arranged by size:
2777 897 229 228
PstI C_TGCA'G
Cuts at: 0 207 483 1008 1316 2225 2267 2300 2330
Size: 207 276 525 308 909 42 33 30
Cuts at: 2330 2820 2876 3080 3113 3584 4065 4131
Size: 490 56 204 33 471 481 66
Fragments arranged by size:
909 525 490 481 471 308 276 207
204 66 56 42 33 33 30
PvuII CAG'CTG
Cuts at: 0 1065 2220 2325 2634 2871 3181 3609 4131
Size: 1065 1155 105 309 237 310 428 522
Fragments arranged by size:
1155 1065 522 428 310 309 237 105
SacI G_AGCT'C
Cuts at: 0 574 1404 3349 4131
Size: 574 830 1945 782
Fragments arranged by size:
1945 830 782 574
SanDI GG'GwC_CC
Cuts at: 0 535 4131
Size: 535 3596
SapI GCTCTTCn'nnn_
Cuts at: 0 2147 2964 3131 4131
Size: 2147 817 167 1000
Fragments arranged by size:
2147 1000 817 167
SexAI A'CCwGG_T
Cuts at: 0 122 4131
Size: 122 4009
SfcI C'TryA_G
Cuts at: 0 203 223 465 479 886 1004 1312 1623
Size: 203 20 242 14 407 118 308 311
Cuts at: 1623 2221 2263 2296 2326 2759 2816 2872 3076
Size: 598 42 33 30 433 57 56 204
Cuts at: 3076 3109 3580 4061 4131
Size: 33 471 481 70
Fragments arranged by size:
598 481 471 433 407 311 308 242
204 203 118 70 57 56 42 33
33 30 20 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1346 4131
Size: 1346 2785
SfoI GGC'GCC
Cuts at: 0 720 4131
Size: 720 3411
SgrAI Cr'CCGG_yG
Cuts at: 0 1778 4131
Size: 1778 2353
SmaI CCC'GGG
Cuts at: 0 2611 4131
Size: 2611 1520
SmlI C'TyrA_G
Cuts at: 0 1377 2059 2188 3847 4131
Size: 1377 682 129 1659 284
Fragments arranged by size:
1659 1377 682 284 129
StuI AGG'CCT
Cuts at: 0 236 963 1967 2703 4131
Size: 236 727 1004 736 1428
Fragments arranged by size:
1428 1004 736 727 236
StyI C'CwwG_G
Cuts at: 0 50 68 1491 2345 3729 4131
Size: 50 18 1423 854 1384 402
Fragments arranged by size:
1423 1384 854 402 50 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 828 1045 1558 1575 2029 2104 3046 4131
Size: 828 217 513 17 454 75 942 1085
Fragments arranged by size:
1085 942 828 513 454 217 75 17
TatI w'GTAC_w
Cuts at: 0 3929 4040 4131
Size: 3929 111 91
Fragments arranged by size:
3929 111 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 1116 1148 1419 1451 3113 3145
Size: 824 32 260 32 271 32 1662 32
Cuts at: 3145 4131
Size: 986
Fragments arranged by size:
1662 986 824 271 260 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 28 876 1932 4131
Size: 28 848 1056 2199
Fragments arranged by size:
2199 1056 848 28
XmaI C'CCGG_G
Cuts at: 0 2609 4131
Size: 2609 1522
XmnI GAAnn'nnTTC
Cuts at: 0 1284 4131
Size: 1284 2847
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SanDI SapI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AgeI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BmgBI BsaBI BsaXI BsiWI BsrGI BssHII BstBI BstEII
BstZ17I ClaI CspCI DraI DrdI EagI EcoRI EcoRV
FspAI HpaI MfeI MluI NdeI NotI NruI NsiI
PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SbfI ScaI SgfI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI