Restriction map for
pF1KSDA0639
(Linear) (Six-base) MAPSORT of: KIAA0639.seq Check: 1523 from: 1 to: 5478
KIAA0639.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 548 1718 3150 5478
Size: 548 1170 1432 2328
Fragments arranged by size:
2328 1432 1170 548
AclI AA'CG_TT
Cuts at: 0 2032 5478
Size: 2032 3446
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1291 1602 2735 5238 5478
Size: 829 462 311 1133 2503 240
Fragments arranged by size:
2503 1133 829 462 311 240
AfeI AGC'GCT
Cuts at: 0 1989 5478
Size: 1989 3489
AflII C'TTAA_G
Cuts at: 0 2001 5478
Size: 2001 3477
AflIII A'CryG_T
Cuts at: 0 2923 5478
Size: 2923 2555
AhdI GACnn_n'nnGTC
Cuts at: 0 544 1491 2927 5478
Size: 544 947 1436 2551
Fragments arranged by size:
2551 1436 947 544
AleI CACnn'nnGTG
Cuts at: 0 3724 5478
Size: 3724 1754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4492 5478
Size: 4492 986
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4125 4157 5449 5478
Size: 4125 32 1292 29
Fragments arranged by size:
4125 1292 32 29
AlwNI CAG_nnn'CTG
Cuts at: 0 631 793 2649 2681 3390 3490 3577 3997
Size: 631 162 1856 32 709 100 87 420
Cuts at: 3997 5058 5478
Size: 1061 420
Fragments arranged by size:
1856 1061 709 631 420 420 162 100
87 32
ApaI G_GGCC'C
Cuts at: 0 3468 4540 4569 4670 4750 5317 5478
Size: 3468 1072 29 101 80 567 161
Fragments arranged by size:
3468 1072 567 161 101 80 29
ApoI r'AATT_y
Cuts at: 0 455 901 1428 1460 2968 5478
Size: 455 446 527 32 1508 2510
Fragments arranged by size:
2510 1508 527 455 446 32
AseI AT'TA_AT
Cuts at: 0 364 5478
Size: 364 5114
AvaI C'yCGr_G
Cuts at: 0 146 952 2419 4807 4909 5010 5037 5356
Size: 146 806 1467 2388 102 101 27 319
Cuts at: 5356 5382 5478
Size: 26 96
Fragments arranged by size:
2388 1467 806 319 146 102 101 96
27 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3470 3503 5478
Size: 3470 33 1975
Fragments arranged by size:
3470 1975 33
BamHI G'GATC_C
Cuts at: 0 1674 5478
Size: 1674 3804
BanI G'GyrC_C
Cuts at: 0 4812 4920 5041 5128 5350 5478
Size: 4812 108 121 87 222 128
Fragments arranged by size:
4812 222 128 121 108 87
BanII G_rGCy'C
Cuts at: 0 3198 3468 4540 4569 4670 4750 4808 4862
Size: 3198 270 1072 29 101 80 58 54
Cuts at: 4862 4888 5204 5317 5389 5478
Size: 26 316 113 72 89
Fragments arranged by size:
3198 1072 316 270 113 101 89 80
72 58 54 29 26
BbeI G_GCGC'C
Cuts at: 0 4816 4924 5045 5132 5478
Size: 4816 108 121 87 346
Fragments arranged by size:
4816 346 121 108 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 790 1304 2406 2813 2923 3239 5478
Size: 790 514 1102 407 110 316 2239
Fragments arranged by size:
2239 1102 790 514 407 316 110
BbvCI CC'TCA_GC
Cuts at: 0 2015 2056 2111 3704 5478
Size: 2015 41 55 1593 1774
Fragments arranged by size:
2015 1774 1593 55 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4576 4610 5008 5042 5172 5206 5478
Size: 4576 34 398 34 130 34 272
Fragments arranged by size:
4576 398 272 130 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 26 4251 5478
Size: 26 4225 1227
Fragments arranged by size:
4225 1227 26
BclI T'GATC_A
Cuts at: 0 174 2772 5478
Size: 174 2598 2706
Fragments arranged by size:
2706 2598 174
BfrBI ATG'CAT
Cuts at: 0 289 3292 5478
Size: 289 3003 2186
Fragments arranged by size:
3003 2186 289
BglI GCCn_nnn'nGGC
Cuts at: 0 979 4798 5359 5478
Size: 979 3819 561 119
Fragments arranged by size:
3819 979 561 119
BglII A'GATC_T
Cuts at: 0 4179 4580 5478
Size: 4179 401 898
Fragments arranged by size:
4179 898 401
BlpI GC'TnA_GC
Cuts at: 0 1991 3709 3769 4523 5478
Size: 1991 1718 60 754 955
Fragments arranged by size:
1991 1718 955 754 60
Bme1580I G_kGCm'C
Cuts at: 0 346 3468 3721 4466 4540 4569 4670 4750
Size: 346 3122 253 745 74 29 101 80
Cuts at: 4750 5185 5317 5355 5478
Size: 435 132 38 123
Fragments arranged by size:
3122 745 435 346 253 132 123 101
80 74 38 29
BmgBI CAC'GTC
Cuts at: 0 1493 4545 5478
Size: 1493 3052 933
Fragments arranged by size:
3052 1493 933
BmrI ACTGGGnnnn_n'
Cuts at: 0 2326 2642 3478 3611 5478
Size: 2326 316 836 133 1867
Fragments arranged by size:
2326 1867 836 316 133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4986 5478
Size: 4986 492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1154 1545 1716 1897 2148 3412 4298
Size: 746 408 391 171 181 251 1264 886
Cuts at: 4298 5478
Size: 1180
Fragments arranged by size:
1264 1180 886 746 408 391 251 181
171
Bpu10I CC'TnA_GC
Cuts at: 0 2015 2056 2111 3704 5478
Size: 2015 41 55 1593 1774
Fragments arranged by size:
2015 1774 1593 55 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 4378 5478
Size: 1306 3072 1100
Fragments arranged by size:
3072 1306 1100
BsaI GGTCTCn'nnnn_
Cuts at: 0 470 2285 2574 2578 3205 5478
Size: 470 1815 289 4 627 2273
Fragments arranged by size:
2273 1815 627 470 289 4
BsaAI yAC'GTr
Cuts at: 0 2502 2926 5157 5186 5478
Size: 2502 424 2231 29 292
Fragments arranged by size:
2502 2231 424 292 29
BsaHI Gr'CG_yC
Cuts at: 0 4739 4813 4921 5042 5129 5478
Size: 4739 74 108 121 87 349
Fragments arranged by size:
4739 349 121 108 87 74
BsaWI w'CCGG_w
Cuts at: 0 1919 4650 4943 5459 5478
Size: 1919 2731 293 516 19
Fragments arranged by size:
2731 1919 516 293 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 719 749 1711 1741 2713 2743 3385 3415
Size: 719 30 962 30 972 30 642 30
Cuts at: 3415 3836 3866 4125 4155 4632 4662 4702 4732
Size: 421 30 259 30 477 30 40 30
Cuts at: 4732 5478
Size: 746
Fragments arranged by size:
972 962 746 719 642 477 421 259
40 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 1249 1270 1508 1719 2032 2128 2718
Size: 664 585 21 238 211 313 96 590
Cuts at: 2718 2721 3430 3631 4146 4381 4531 4870 5478
Size: 3 709 201 515 235 150 339 608
Fragments arranged by size:
709 664 608 590 585 515 339 313
238 235 211 201 150 96 21 3
BseYI C'CCAG_C
Cuts at: 0 1368 1984 3468 3990 4672 4871 4990 5290
Size: 1368 616 1484 522 682 199 119 300
Cuts at: 5290 5346 5478
Size: 56 132
Fragments arranged by size:
1484 1368 682 616 522 300 199 132
119 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1856 2482 3133 4498 4573 5478
Size: 1856 626 651 1365 75 905
Fragments arranged by size:
1856 1365 905 651 626 75
BsiEI CG_ry'CG
Cuts at: 0 3831 4438 4844 4893 5478
Size: 3831 607 406 49 585
Fragments arranged by size:
3831 607 585 406 49
BsiHKAI G_wGCw'C
Cuts at: 0 1904 5204 5478
Size: 1904 3300 274
Fragments arranged by size:
3300 1904 274
BsmI GAATG_Cn'
Cuts at: 0 273 1365 5031 5478
Size: 273 1092 3666 447
Fragments arranged by size:
3666 1092 447 273
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4445 4722 5478
Size: 4445 277 756
Fragments arranged by size:
4445 756 277
Bsp1286I G_dGCh'C
Cuts at: 0 346 1904 3198 3468 3721 4466 4540 4569
Size: 346 1558 1294 270 253 745 74 29
Cuts at: 4569 4670 4750 4808 4862 4888 5185 5204 5317
Size: 101 80 58 54 26 297 19 113
Cuts at: 5317 5355 5389 5478
Size: 38 34 89
Fragments arranged by size:
1558 1294 745 346 297 270 253 113
101 89 80 74 58 54 38 34
29 26 19
BspEI T'CCGG_A
Cuts at: 0 1919 4650 4943 5478
Size: 1919 2731 293 535
Fragments arranged by size:
2731 1919 535 293
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 211 2479 4602 5216 5478
Size: 211 2268 2123 614 262
Fragments arranged by size:
2268 2123 614 262 211
BsrBI CCG'CTC
Cuts at: 0 3189 3735 5478
Size: 3189 546 1743
Fragments arranged by size:
3189 1743 546
BsrDI GCAATG_nn'
Cuts at: 0 1166 1815 2105 2775 2787 5478
Size: 1166 649 290 670 12 2691
Fragments arranged by size:
2691 1166 670 649 290 12
BsrFI r'CCGG_y
Cuts at: 0 47 1184 2697 4217 5031 5478
Size: 47 1137 1513 1520 814 447
Fragments arranged by size:
1520 1513 1137 814 447 47
BssSI C'ACGA_G
Cuts at: 0 4879 5478
Size: 4879 599
BstEII G'GTnAC_C
Cuts at: 0 1785 4503 4638 5478
Size: 1785 2718 135 840
Fragments arranged by size:
2718 1785 840 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 78 2085 2964 3476 5400 5478
Size: 78 2007 879 512 1924 78
Fragments arranged by size:
2007 1924 879 512 78 78
BstYI r'GATC_y
Cuts at: 0 1107 1674 4179 4317 4580 5478
Size: 1107 567 2505 138 263 898
Fragments arranged by size:
2505 1107 898 567 263 138
BstZ17I GTA'TAC
Cuts at: 0 3151 5478
Size: 3151 2327
Bsu36I CC'TnA_GG
Cuts at: 0 4030 4796 5214 5478
Size: 4030 766 418 264
Fragments arranged by size:
4030 766 418 264
BtgI C'CryG_G
Cuts at: 0 974 1581 3686 3725 4838 5123 5309 5478
Size: 974 607 2105 39 1113 285 186 169
Fragments arranged by size:
2105 1113 974 607 285 186 169 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2567 4430 5478
Size: 2567 1863 1048
Fragments arranged by size:
2567 1863 1048
BtsI GCAGTG_nn'
Cuts at: 0 387 774 2754 3456 4097 5478
Size: 387 387 1980 702 641 1381
Fragments arranged by size:
1980 1381 702 641 387 387
DraI TTT'AAA
Cuts at: 0 432 1400 5478
Size: 432 968 4078
Fragments arranged by size:
4078 968 432
DraIII CAC_nnn'GTG
Cuts at: 0 1071 2661 5478
Size: 1071 1590 2817
Fragments arranged by size:
2817 1590 1071
EaeI y'GGCC_r
Cuts at: 0 2405 3828 4435 4841 5423 5478
Size: 2405 1423 607 406 582 55
Fragments arranged by size:
2405 1423 607 582 406 55
EagI C'GGCC_G
Cuts at: 0 3828 4435 4841 5478
Size: 3828 607 406 637
Fragments arranged by size:
3828 637 607 406
EarI CTCTTCn'nnn_
Cuts at: 0 1310 2201 2739 3221 3608 4417 5478
Size: 1310 891 538 482 387 809 1061
Fragments arranged by size:
1310 1061 891 809 538 482 387
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 5379 5478
Size: 5379 99
EcoICRI GAG'CTC
Cuts at: 0 5202 5478
Size: 5202 276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 829 1154 1291 1545 1602 1716 1897
Size: 746 83 325 137 254 57 114 181
Cuts at: 1897 2148 2735 3412 4298 5238 5478
Size: 251 587 677 886 940 240
Fragments arranged by size:
940 886 746 677 587 325 254 251
240 181 137 114 83 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2299 3412 5425 5478
Size: 2299 1113 2013 53
Fragments arranged by size:
2299 2013 1113 53
EcoO109I rG'GnC_Cy
Cuts at: 0 538 1083 1797 3464 3465 4537 4565 4566
Size: 538 545 714 1667 1 1072 28 1
Cuts at: 4566 4667 4747 4914 4995 5055 5088 5161 5314
Size: 101 80 167 81 60 33 73 153
Cuts at: 5314 5478
Size: 164
Fragments arranged by size:
1667 1072 714 545 538 167 164 153
101 81 80 73 60 33 28 1
1
EcoRI G'AATT_C
Cuts at: 0 1460 2968 5478
Size: 1460 1508 2510
Fragments arranged by size:
2510 1508 1460
EcoRV GAT'ATC
Cuts at: 0 1440 5478
Size: 1440 4038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3403 4419 5268 5478
Size: 3403 1016 849 210
Fragments arranged by size:
3403 1016 849 210
HaeII r_GCGC'y
Cuts at: 0 794 1884 1991 4816 4902 4924 5045 5132
Size: 794 1090 107 2825 86 22 121 87
Cuts at: 5132 5375 5478
Size: 243 103
Fragments arranged by size:
2825 1090 794 243 121 107 103 87
86 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 242 274 530 562 577 609 697 729
Size: 242 32 256 32 15 32 88 32
Cuts at: 729 1477 1509 1711 1743 2694 2713 2726 2745
Size: 748 32 202 32 951 19 13 19
Cuts at: 2745 2913 2945 3080 3112 3532 3564 3855 3887
Size: 168 32 135 32 420 32 291 32
Cuts at: 3887 4053 4085 4166 4198 4875 4907 5197 5229
Size: 166 32 81 32 677 32 290 32
Cuts at: 5229 5478
Size: 249
Fragments arranged by size:
951 748 677 420 291 290 256 249
242 202 168 166 135 88 81 32
32 32 32 32 32 32 32 32
32 32 32 32 32 19 19 15
13
HincII GTy'rAC
Cuts at: 0 549 1755 2796 3404 4106 5478
Size: 549 1206 1041 608 702 1372
Fragments arranged by size:
1372 1206 1041 702 608 549
HindIII A'AGCT_T
Cuts at: 0 3334 5478
Size: 3334 2144
HpaI GTT'AAC
Cuts at: 0 1755 2796 4106 5478
Size: 1755 1041 1310 1372
Fragments arranged by size:
1755 1372 1310 1041
KasI G'GCGC_C
Cuts at: 0 4812 4920 5041 5128 5478
Size: 4812 108 121 87 350
Fragments arranged by size:
4812 350 121 108 87
MfeI C'AATT_G
Cuts at: 0 2843 3325 5478
Size: 2843 482 2153
Fragments arranged by size:
2843 2153 482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1467 2261 4715 5478
Size: 1467 794 2454 763
Fragments arranged by size:
2454 1467 794 763
MscI TGG'CCA
Cuts at: 0 2407 5425 5478
Size: 2407 3018 53
Fragments arranged by size:
3018 2407 53
MslI CAynn'nnrTG
Cuts at: 0 2401 2780 3724 4474 4501 5478
Size: 2401 379 944 750 27 977
Fragments arranged by size:
2401 977 944 750 379 27
MspA1I CmG'CkG
Cuts at: 0 349 1000 1143 1710 3688 4636 5416 5478
Size: 349 651 143 567 1978 948 780 62
Fragments arranged by size:
1978 948 780 651 567 349 143 62
NaeI GCC'GGC
Cuts at: 0 5033 5478
Size: 5033 445
NarI GG'CG_CC
Cuts at: 0 4813 4921 5042 5129 5478
Size: 4813 108 121 87 349
Fragments arranged by size:
4813 349 121 108 87
NcoI C'CATG_G
Cuts at: 0 974 1581 5478
Size: 974 607 3897
Fragments arranged by size:
3897 974 607
NgoMIV G'CCGG_C
Cuts at: 0 5031 5478
Size: 5031 447
NsiI A_TGCA'T
Cuts at: 0 291 3294 5478
Size: 291 3003 2184
Fragments arranged by size:
3003 2184 291
NspI r_CATG'y
Cuts at: 0 293 3789 4986 5478
Size: 293 3496 1197 492
Fragments arranged by size:
3496 1197 492 293
PasI CC'CwG_GG
Cuts at: 0 1800 3936 4743 5478
Size: 1800 2136 807 735
Fragments arranged by size:
2136 1800 807 735
PflMI CCAn_nnn'nTGG
Cuts at: 0 1071 3490 3577 3942 5478
Size: 1071 2419 87 365 1536
Fragments arranged by size:
2419 1536 1071 365 87
PfoI T'CCnGG_A
Cuts at: 0 1103 1112 1133 1211 3082 3521 4320 5090
Size: 1103 9 21 78 1871 439 799 770
Cuts at: 5090 5478
Size: 388
Fragments arranged by size:
1871 1103 799 770 439 388 78 21
9
PmlI CAC'GTG
Cuts at: 0 2502 5186 5478
Size: 2502 2684 292
Fragments arranged by size:
2684 2502 292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3724 3756 4125 4157 5098 5130 5478
Size: 3724 32 369 32 941 32 348
Fragments arranged by size:
3724 941 369 348 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 538 1083 4995 5088 5478
Size: 538 545 3912 93 390
Fragments arranged by size:
3912 545 538 390 93
PsiI TTA'TAA
Cuts at: 0 461 5478
Size: 461 5017
PspOMI G'GGCC_C
Cuts at: 0 3464 4536 4565 4666 4746 5313 5478
Size: 3464 1072 29 101 80 567 165
Fragments arranged by size:
3464 1072 567 165 101 80 29
PspXI vC'TCGA_Gb
Cuts at: 0 2419 4909 5478
Size: 2419 2490 569
Fragments arranged by size:
2490 2419 569
PstI C_TGCA'G
Cuts at: 0 266 798 999 5478
Size: 266 532 201 4479
Fragments arranged by size:
4479 532 266 201
PvuII CAG'CTG
Cuts at: 0 349 1000 1143 1710 5416 5478
Size: 349 651 143 567 3706 62
Fragments arranged by size:
3706 651 567 349 143 62
SacI G_AGCT'C
Cuts at: 0 5204 5478
Size: 5204 274
SacII CC_GC'GG
Cuts at: 0 3689 5478
Size: 3689 1789
SalI G'TCGA_C
Cuts at: 0 547 5478
Size: 547 4931
SanDI GG'GwC_CC
Cuts at: 0 4995 5478
Size: 4995 483
ScaI AGT'ACT
Cuts at: 0 3313 5251 5478
Size: 3313 1938 227
Fragments arranged by size:
3313 1938 227
SfcI C'TryA_G
Cuts at: 0 262 794 995 2149 3017 4046 5478
Size: 262 532 201 1154 868 1029 1432
Fragments arranged by size:
1432 1154 1029 868 532 262 201
SfoI GGC'GCC
Cuts at: 0 4814 4922 5043 5130 5478
Size: 4814 108 121 87 348
Fragments arranged by size:
4814 348 121 108 87
SmaI CCC'GGG
Cuts at: 0 148 5012 5039 5478
Size: 148 4864 27 439
Fragments arranged by size:
4864 439 148 27
SmlI C'TyrA_G
Cuts at: 0 1285 2001 2419 4393 4909 5478
Size: 1285 716 418 1974 516 569
Fragments arranged by size:
1974 1285 716 569 516 418
SphI G_CATG'C
Cuts at: 0 4986 5478
Size: 4986 492
SspI AAT'ATT
Cuts at: 0 3921 5478
Size: 3921 1557
StyI C'CwwG_G
Cuts at: 0 246 974 1581 5426 5478
Size: 246 728 607 3845 52
Fragments arranged by size:
3845 728 607 246 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 838 5338 5478
Size: 838 4500 140
Fragments arranged by size:
4500 838 140
TatI w'GTAC_w
Cuts at: 0 1391 3311 5249 5478
Size: 1391 1920 1938 229
Fragments arranged by size:
1938 1920 1391 229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 719 751 2301 2333 3836 3868 4632 4664
Size: 719 32 1550 32 1503 32 764 32
Cuts at: 4664 4702 4734 5478
Size: 38 32 744
Fragments arranged by size:
1550 1503 764 744 719 38 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4731 5086 5478
Size: 4731 355 392
Fragments arranged by size:
4731 392 355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 78 1797 5478
Size: 18 60 1719 3681
Fragments arranged by size:
3681 1719 60 18
XhoI C'TCGA_G
Cuts at: 0 2419 4909 5478
Size: 2419 2490 569
Fragments arranged by size:
2490 2419 569
XmaI C'CCGG_G
Cuts at: 0 146 5010 5037 5478
Size: 146 4864 27 441
Fragments arranged by size:
4864 441 146 27
XmnI GAAnn'nnTTC
Cuts at: 0 470 5478
Size: 470 5008
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApoI AseI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII
SacI SacII SalI SanDI ScaI SfcI SfoI SmaI
SmlI SphI SspI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AgeI ApaLI AscI AsiSI AvrII
BmtI BsaBI BsiWI BspHI BsrGI BssHII BstAPI BstBI
ClaI CspCI DrdI FseI FspI FspAI KpnI MluI
NdeI NheI NotI NruI PacI PciI PmeI PshAI
PsrI PvuI RsrII SapI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI StuI SwaI XbaI ZraI