Restriction map for
pF1KA0656
(Linear) (Six-base) MAPSORT of: KIAA0656.seq Check: 4781 from: 1 to: 2721
KIAA0656.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1122 2658 2721
Size: 1122 1536 63
Fragments arranged by size:
1536 1122 63
AccI GT'mk_AC
Cuts at: 0 361 2721
Size: 361 2360
Acc65I G'GTAC_C
Cuts at: 0 2220 2314 2721
Size: 2220 94 407
Fragments arranged by size:
2220 407 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1208 1325 1469 1736 1889 2323 2721
Size: 1208 117 144 267 153 434 398
Fragments arranged by size:
1208 434 398 267 153 144 117
AfeI AGC'GCT
Cuts at: 0 1947 2721
Size: 1947 774
AgeI A'CCGG_T
Cuts at: 0 978 2721
Size: 978 1743
AlwNI CAG_nnn'CTG
Cuts at: 0 58 212 1267 1465 1594 1744 2721
Size: 58 154 1055 198 129 150 977
Fragments arranged by size:
1055 977 198 154 150 129 58
ApaI G_GGCC'C
Cuts at: 0 109 2490 2721
Size: 109 2381 231
Fragments arranged by size:
2381 231 109
ApaLI G'TGCA_C
Cuts at: 0 2432 2721
Size: 2432 289
ApoI r'AATT_y
Cuts at: 0 538 704 745 2306 2721
Size: 538 166 41 1561 415
Fragments arranged by size:
1561 538 415 166 41
BamHI G'GATC_C
Cuts at: 0 340 2721
Size: 340 2381
BanI G'GyrC_C
Cuts at: 0 2034 2187 2220 2314 2721
Size: 2034 153 33 94 407
Fragments arranged by size:
2034 407 153 94 33
BanII G_rGCy'C
Cuts at: 0 109 2490 2721
Size: 109 2381 231
Fragments arranged by size:
2381 231 109
BbeI G_GCGC'C
Cuts at: 0 2038 2721
Size: 2038 683
BbsI GAAGACnn'nnnn_
Cuts at: 0 905 2721
Size: 905 1816
BbvCI CC'TCA_GC
Cuts at: 0 1260 2558 2721
Size: 1260 1298 163
Fragments arranged by size:
1298 1260 163
BfrBI ATG'CAT
Cuts at: 0 1417 1852 1933 2721
Size: 1417 435 81 788
Fragments arranged by size:
1417 788 435 81
BglII A'GATC_T
Cuts at: 0 606 2334 2721
Size: 606 1728 387
Fragments arranged by size:
1728 606 387
Bme1580I G_kGCm'C
Cuts at: 0 109 2436 2490 2721
Size: 109 2327 54 231
Fragments arranged by size:
2327 231 109 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 1009 2222 2721
Size: 1009 1213 499
Fragments arranged by size:
1213 1009 499
BmtI G_CTAG'C
Cuts at: 0 1538 2721
Size: 1538 1183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1039 1094 1139 2033 2042 2372 2384
Size: 902 137 55 45 894 9 330 12
Cuts at: 2384 2528 2660 2721
Size: 144 132 61
Fragments arranged by size:
902 894 330 144 137 132 61 55
45 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 1260 1472 1733 2558 2721
Size: 1260 212 261 825 163
Fragments arranged by size:
1260 825 261 212 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 2721
Size: 736 1985
BsaI GGTCTCn'nnnn_
Cuts at: 0 145 1133 1498 2721
Size: 145 988 365 1223
Fragments arranged by size:
1223 988 365 145
BsaBI GATnn'nnATC
Cuts at: 0 548 2721
Size: 548 2173
BsaHI Gr'CG_yC
Cuts at: 0 2035 2721
Size: 2035 686
BsaWI w'CCGG_w
Cuts at: 0 978 1548 2721
Size: 978 570 1173
Fragments arranged by size:
1173 978 570
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 462 492 792 822 1530 1560 1653 1656
Size: 462 30 300 30 708 30 93 3
Cuts at: 1656 1683 1686 2721
Size: 27 3 1035
Fragments arranged by size:
1035 708 462 300 93 30 30 30
27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1136 1661 1773 2721
Size: 1136 525 112 948
Fragments arranged by size:
1136 948 525 112
BseYI C'CCAG_C
Cuts at: 0 212 811 2194 2533 2721
Size: 212 599 1383 339 188
Fragments arranged by size:
1383 599 339 212 188
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 1451 1478 2189 2721
Size: 1151 300 27 711 532
Fragments arranged by size:
1151 711 532 300 27
BsiHKAI G_wGCw'C
Cuts at: 0 894 2436 2721
Size: 894 1542 285
Fragments arranged by size:
1542 894 285
BsmI GAATG_Cn'
Cuts at: 0 2352 2528 2721
Size: 2352 176 193
Fragments arranged by size:
2352 193 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 820 941 2721
Size: 820 121 1780
Fragments arranged by size:
1780 820 121
Bsp1286I G_dGCh'C
Cuts at: 0 109 894 2436 2490 2721
Size: 109 785 1542 54 231
Fragments arranged by size:
1542 785 231 109 54
BspHI T'CATG_A
Cuts at: 0 93 2721
Size: 93 2628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1122 1971 2081 2658 2721
Size: 1122 849 110 577 63
Fragments arranged by size:
1122 849 577 110 63
BsrBI CCG'CTC
Cuts at: 0 37 73 195 2721
Size: 37 36 122 2526
Fragments arranged by size:
2526 122 37 36
BsrDI GCAATG_nn'
Cuts at: 0 1496 2243 2721
Size: 1496 747 478
Fragments arranged by size:
1496 747 478
BsrFI r'CCGG_y
Cuts at: 0 978 2565 2721
Size: 978 1587 156
Fragments arranged by size:
1587 978 156
BssSI C'ACGA_G
Cuts at: 0 734 2721
Size: 734 1987
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1414 1465 1594 1879 2721
Size: 1414 51 129 285 842
Fragments arranged by size:
1414 842 285 129 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2408 2721
Size: 2408 313
BstYI r'GATC_y
Cuts at: 0 340 606 883 1203 1311 2334 2697 2721
Size: 340 266 277 320 108 1023 363 24
Fragments arranged by size:
1023 363 340 320 277 266 108 24
BtgI C'CryG_G
Cuts at: 0 345 2183 2721
Size: 345 1838 538
Fragments arranged by size:
1838 538 345
BtsI GCAGTG_nn'
Cuts at: 0 529 1127 1298 1664 1826 1877 2721
Size: 529 598 171 366 162 51 844
Fragments arranged by size:
844 598 529 366 171 162 51
DraIII CAC_nnn'GTG
Cuts at: 0 2431 2721
Size: 2431 290
DrdI GACnn_nn'nnGTC
Cuts at: 0 738 2721
Size: 738 1983
EaeI y'GGCC_r
Cuts at: 0 176 2721
Size: 176 2545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1039 1094 1139 1208 1325 1469 1736
Size: 902 137 55 45 69 117 144 267
Cuts at: 1736 1889 2033 2042 2323 2372 2384 2528 2660
Size: 153 144 9 281 49 12 144 132
Cuts at: 2660 2721
Size: 61
Fragments arranged by size:
902 281 267 153 144 144 144 137
132 117 69 61 55 49 45 12
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 369 802 1118 2441 2510 2721
Size: 369 433 316 1323 69 211
Fragments arranged by size:
1323 433 369 316 211 69
EcoO109I rG'GnC_Cy
Cuts at: 0 106 1350 1799 2486 2487 2594 2685 2721
Size: 106 1244 449 687 1 107 91 36
Fragments arranged by size:
1244 687 449 107 106 91 36 1
EcoRV GAT'ATC
Cuts at: 0 1686 2721
Size: 1686 1035
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 929 1964 2453 2721
Size: 929 1035 489 268
Fragments arranged by size:
1035 929 489 268
HaeII r_GCGC'y
Cuts at: 0 378 1732 1949 2038 2721
Size: 378 1354 217 89 683
Fragments arranged by size:
1354 683 378 217 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 231 263 462 494 1054 1086 1188 1220
Size: 231 32 199 32 560 32 102 32
Cuts at: 1220 1765 1797 1825 1857 2046 2078 2721
Size: 545 32 28 32 189 32 643
Fragments arranged by size:
643 560 545 231 199 189 102 32
32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 0 2366 2721
Size: 2366 355
KasI G'GCGC_C
Cuts at: 0 2034 2721
Size: 2034 687
KpnI G_GTAC'C
Cuts at: 0 2224 2318 2721
Size: 2224 94 403
Fragments arranged by size:
2224 403 94
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2478 2482 2721
Size: 2478 4 239
Fragments arranged by size:
2478 239 4
MslI CAynn'nnrTG
Cuts at: 0 266 350 554 2548 2721
Size: 266 84 204 1994 173
Fragments arranged by size:
1994 266 204 173 84
MspA1I CmG'CkG
Cuts at: 0 1591 1624 1651 2194 2614 2721
Size: 1591 33 27 543 420 107
Fragments arranged by size:
1591 543 420 107 33 27
NarI GG'CG_CC
Cuts at: 0 2035 2721
Size: 2035 686
NcoI C'CATG_G
Cuts at: 0 345 2183 2721
Size: 345 1838 538
Fragments arranged by size:
1838 538 345
NheI G'CTAG_C
Cuts at: 0 1534 2721
Size: 1534 1187
NsiI A_TGCA'T
Cuts at: 0 1419 1854 1935 2721
Size: 1419 435 81 786
Fragments arranged by size:
1419 786 435 81
NspI r_CATG'y
Cuts at: 0 1421 2547 2721
Size: 1421 1126 174
Fragments arranged by size:
1421 1126 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 1117 1133 2497 2721
Size: 1117 16 1364 224
Fragments arranged by size:
1364 1117 224 16
PfoI T'CCnGG_A
Cuts at: 0 1046 2721
Size: 1046 1675
PpuMI rG'GwC_Cy
Cuts at: 0 2685 2721
Size: 2685 36
PspOMI G'GGCC_C
Cuts at: 0 105 2486 2721
Size: 105 2381 235
Fragments arranged by size:
2381 235 105
PstI C_TGCA'G
Cuts at: 0 1359 1728 2721
Size: 1359 369 993
Fragments arranged by size:
1359 993 369
PvuII CAG'CTG
Cuts at: 0 1591 2194 2721
Size: 1591 603 527
Fragments arranged by size:
1591 603 527
SexAI A'CCwGG_T
Cuts at: 0 2407 2721
Size: 2407 314
SfcI C'TryA_G
Cuts at: 0 62 378 511 1355 1397 1517 1529 1724
Size: 62 316 133 844 42 120 12 195
Cuts at: 1724 2077 2721
Size: 353 644
Fragments arranged by size:
844 644 353 316 195 133 120 62
42 12
SfoI GGC'GCC
Cuts at: 0 2036 2721
Size: 2036 685
SmlI C'TyrA_G
Cuts at: 0 751 2721
Size: 751 1970
SphI G_CATG'C
Cuts at: 0 2547 2721
Size: 2547 174
SspI AAT'ATT
Cuts at: 0 165 286 2721
Size: 165 121 2435
Fragments arranged by size:
2435 165 121
StyI C'CwwG_G
Cuts at: 0 345 2183 2256 2721
Size: 345 1838 73 465
Fragments arranged by size:
1838 465 345 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 204 2241 2721
Size: 204 2037 480
Fragments arranged by size:
2037 480 204
TatI w'GTAC_w
Cuts at: 0 41 1768 2721
Size: 41 1727 953
Fragments arranged by size:
1727 953 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 792 824 1530 1562 1653 1656 1685 1688
Size: 792 32 706 32 91 3 29 3
Cuts at: 1688 2721
Size: 1033
Fragments arranged by size:
1033 792 706 91 32 32 29 3
3
XbaI T'CTAG_A
Cuts at: 0 295 699 1680 2721
Size: 295 404 981 1041
Fragments arranged by size:
1041 981 404 295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 819 2235 2721
Size: 819 1416 486
Fragments arranged by size:
1416 819 486
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AgeI AlwNI ApaI
ApaLI ApoI BamHI BanI BanII BbeI BbsI BbvCI
BfrBI BglII Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI DraIII
DrdI EaeI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII KasI KpnI MmeI MslI MspA1I NarI
NcoI NheI NsiI NspI PflMI PfoI PpuMI PspOMI
PstI PvuII SexAI SfcI SfoI SmlI SphI SspI
StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AatII AclI AflII AflIII AhdI AleI AlfI AloI
AscI AseI AsiSI AvaI AvrII BaeI BcgI BciVI
BclI BglI BlpI BmgBI BplI BsaAI BsiEI BsiWI
BspEI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI EagI EarI EciI EcoICRI EcoRI
FseI FspI FspAI HindIII HpaI MfeI MluI MscI
NaeI NdeI NgoMIV NotI NruI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI Tth111I XhoI XmaI XmnI ZraI