Restriction map for
pF1KSDA0658
(Linear) (Six-base) MAPSORT of: KIAA0658.seq Check: 5452 from: 1 to: 1779
KIAA0658.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 1551 1779
Size: 1551 228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 683 1779
Size: 208 475 1096
Fragments arranged by size:
1096 475 208
AfeI AGC'GCT
Cuts at: 0 511 1779
Size: 511 1268
AflIII A'CryG_T
Cuts at: 0 302 1779
Size: 302 1477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 109 556 1449 1779
Size: 109 447 893 330
Fragments arranged by size:
893 447 330 109
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 766 798 1779
Size: 637 32 97 32 981
Fragments arranged by size:
981 637 97 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 231 1779
Size: 231 1548
ApaI G_GGCC'C
Cuts at: 0 1734 1779
Size: 1734 45
ApaLI G'TGCA_C
Cuts at: 0 73 1779
Size: 73 1706
ApoI r'AATT_y
Cuts at: 0 438 1779
Size: 438 1341
AvaI C'yCGr_G
Cuts at: 0 1611 1733 1779
Size: 1611 122 46
Fragments arranged by size:
1611 122 46
BamHI G'GATC_C
Cuts at: 0 1112 1779
Size: 1112 667
BanI G'GyrC_C
Cuts at: 0 39 1779
Size: 39 1740
BanII G_rGCy'C
Cuts at: 0 1397 1607 1734 1779
Size: 1397 210 127 45
Fragments arranged by size:
1397 210 127 45
BbeI G_GCGC'C
Cuts at: 0 43 1779
Size: 43 1736
BbsI GAAGACnn'nnnn_
Cuts at: 0 1586 1779
Size: 1586 193
BbvCI CC'TCA_GC
Cuts at: 0 808 1049 1779
Size: 808 241 730
Fragments arranged by size:
808 730 241
BglI GCCn_nnn'nGGC
Cuts at: 0 412 803 1045 1304 1622 1738 1779
Size: 412 391 242 259 318 116 41
Fragments arranged by size:
412 391 318 259 242 116 41
Bme1580I G_kGCm'C
Cuts at: 0 77 635 1734 1779
Size: 77 558 1099 45
Fragments arranged by size:
1099 558 77 45
BmgBI CAC'GTC
Cuts at: 0 303 1779
Size: 303 1476
BmrI ACTGGGnnnn_n'
Cuts at: 0 1322 1652 1779
Size: 1322 330 127
Fragments arranged by size:
1322 330 127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1626 1779
Size: 1626 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1779
Size: 661 1118
Bpu10I CC'TnA_GC
Cuts at: 0 808 1049 1779
Size: 808 241 730
Fragments arranged by size:
808 730 241
BsaI GGTCTCn'nnnn_
Cuts at: 0 613 756 1474 1779
Size: 613 143 718 305
Fragments arranged by size:
718 613 305 143
BsaHI Gr'CG_yC
Cuts at: 0 40 1779
Size: 40 1739
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 766 796 1779
Size: 637 30 99 30 983
Fragments arranged by size:
983 637 99 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 177 180 658 707 1779
Size: 177 3 478 49 1072
Fragments arranged by size:
1072 478 177 49 3
BseYI C'CCAG_C
Cuts at: 0 648 1175 1606 1665 1779
Size: 648 527 431 59 114
Fragments arranged by size:
648 527 431 114 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1658 1779
Size: 1658 121
BsiEI CG_ry'CG
Cuts at: 0 128 1779
Size: 128 1651
BsiHKAI G_wGCw'C
Cuts at: 0 77 1779
Size: 77 1702
BsmI GAATG_Cn'
Cuts at: 0 1407 1779
Size: 1407 372
Bsp1286I G_dGCh'C
Cuts at: 0 77 635 1397 1607 1734 1779
Size: 77 558 762 210 127 45
Fragments arranged by size:
762 558 210 127 77 45
BspHI T'CATG_A
Cuts at: 0 395 1085 1779
Size: 395 690 694
Fragments arranged by size:
694 690 395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 827 1361 1779
Size: 827 534 418
Fragments arranged by size:
827 534 418
BsrBI CCG'CTC
Cuts at: 0 1184 1779
Size: 1184 595
BsrFI r'CCGG_y
Cuts at: 0 1487 1779
Size: 1487 292
BssHII G'CGCG_C
Cuts at: 0 139 1779
Size: 139 1640
BstEII G'GTnAC_C
Cuts at: 0 564 1779
Size: 564 1215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 683 1779
Size: 683 1096
BstYI r'GATC_y
Cuts at: 0 599 1007 1112 1779
Size: 599 408 105 667
Fragments arranged by size:
667 599 408 105
Bsu36I CC'TnA_GG
Cuts at: 0 1028 1779
Size: 1028 751
BtgI C'CryG_G
Cuts at: 0 172 1136 1155 1456 1779
Size: 172 964 19 301 323
Fragments arranged by size:
964 323 301 172 19
BtsI GCAGTG_nn'
Cuts at: 0 1269 1287 1641 1779
Size: 1269 18 354 138
Fragments arranged by size:
1269 354 138 18
DraIII CAC_nnn'GTG
Cuts at: 0 1596 1711 1779
Size: 1596 115 68
Fragments arranged by size:
1596 115 68
DrdI GACnn_nn'nnGTC
Cuts at: 0 1168 1779
Size: 1168 611
EaeI y'GGCC_r
Cuts at: 0 125 404 791 1037 1311 1779
Size: 125 279 387 246 274 468
Fragments arranged by size:
468 387 279 274 246 125
EagI C'GGCC_G
Cuts at: 0 125 1779
Size: 125 1654
EarI CTCTTCn'nnn_
Cuts at: 0 72 853 1779
Size: 72 781 926
Fragments arranged by size:
926 781 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 661 683 1779
Size: 208 453 22 1096
Fragments arranged by size:
1096 453 208 22
EcoO109I rG'GnC_Cy
Cuts at: 0 867 1032 1161 1779
Size: 867 165 129 618
Fragments arranged by size:
867 618 165 129
EcoRI G'AATT_C
Cuts at: 0 438 1779
Size: 438 1341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1080 1779
Size: 1080 699
HaeII r_GCGC'y
Cuts at: 0 43 49 65 513 1779
Size: 43 6 16 448 1266
Fragments arranged by size:
1266 448 43 16 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 690 722 1779
Size: 49 32 609 32 1057
Fragments arranged by size:
1057 609 49 32 32
HincII GTy'rAC
Cuts at: 0 1470 1779
Size: 1470 309
KasI G'GCGC_C
Cuts at: 0 39 1779
Size: 39 1740
MscI TGG'CCA
Cuts at: 0 406 793 1039 1779
Size: 406 387 246 740
Fragments arranged by size:
740 406 387 246
MslI CAynn'nnrTG
Cuts at: 0 400 1444 1779
Size: 400 1044 335
Fragments arranged by size:
1044 400 335
MspA1I CmG'CkG
Cuts at: 0 28 679 1157 1175 1244 1779
Size: 28 651 478 18 69 535
Fragments arranged by size:
651 535 478 69 28 18
NaeI GCC'GGC
Cuts at: 0 1489 1779
Size: 1489 290
NarI GG'CG_CC
Cuts at: 0 40 1779
Size: 40 1739
NgoMIV G'CCGG_C
Cuts at: 0 1487 1779
Size: 1487 292
NspI r_CATG'y
Cuts at: 0 1135 1779
Size: 1135 644
PasI CC'CwG_GG
Cuts at: 0 1013 1779
Size: 1013 766
PflMI CCAn_nnn'nTGG
Cuts at: 0 579 1122 1779
Size: 579 543 657
Fragments arranged by size:
657 579 543
PfoI T'CCnGG_A
Cuts at: 0 602 1211 1779
Size: 602 609 568
Fragments arranged by size:
609 602 568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 766 798 1779
Size: 637 32 97 32 981
Fragments arranged by size:
981 637 97 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 867 1161 1779
Size: 867 294 618
Fragments arranged by size:
867 618 294
PshAI GACnn'nnGTC
Cuts at: 0 1586 1779
Size: 1586 193
PspOMI G'GGCC_C
Cuts at: 0 1730 1779
Size: 1730 49
PspXI vC'TCGA_Gb
Cuts at: 0 1611 1779
Size: 1611 168
PstI C_TGCA'G
Cuts at: 0 592 1264 1779
Size: 592 672 515
Fragments arranged by size:
672 592 515
PvuII CAG'CTG
Cuts at: 0 28 679 1175 1244 1779
Size: 28 651 496 69 535
Fragments arranged by size:
651 535 496 69 28
SacII CC_GC'GG
Cuts at: 0 1158 1779
Size: 1158 621
SexAI A'CCwGG_T
Cuts at: 0 1707 1779
Size: 1707 72
SfcI C'TryA_G
Cuts at: 0 588 1260 1779
Size: 588 672 519
Fragments arranged by size:
672 588 519
SfoI GGC'GCC
Cuts at: 0 41 1779
Size: 41 1738
SmaI CCC'GGG
Cuts at: 0 1735 1779
Size: 1735 44
SmlI C'TyrA_G
Cuts at: 0 1611 1779
Size: 1611 168
SphI G_CATG'C
Cuts at: 0 1135 1779
Size: 1135 644
StuI AGG'CCT
Cuts at: 0 742 806 1779
Size: 742 64 973
Fragments arranged by size:
973 742 64
StyI C'CwwG_G
Cuts at: 0 310 407 1069 1779
Size: 310 97 662 710
Fragments arranged by size:
710 662 310 97
Tth111I GACn'n_nGTC
Cuts at: 0 671 1547 1779
Size: 671 876 232
Fragments arranged by size:
876 671 232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 399 552 576 786 1683 1779
Size: 399 153 24 210 897 96
Fragments arranged by size:
897 399 210 153 96 24
XhoI C'TCGA_G
Cuts at: 0 1611 1779
Size: 1611 168
XmaI C'CCGG_G
Cuts at: 0 1733 1779
Size: 1733 46
XmnI GAAnn'nnTTC
Cuts at: 0 1369 1779
Size: 1369 410
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII KasI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuII SacII SexAI SfcI SfoI SmaI
SmlI SphI StuI StyI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BfrBI BglII BlpI BmtI BpuEI BsaAI BsaBI BsaWI
BsiWI BsmBI BspEI BsrDI BsrGI BssSI BstAPI BstBI
BstZ17I BtgZI ClaI CspCI DraI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PsiI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI TaqII TatI
TstI XbaI ZraI