Restriction map for
pF1KSDA0661
(Linear) (Six-base) MAPSORT of: KIAA0661.seq Check: 9684 from: 1 to: 3003
KIAA0661.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1277 2225 3003
Size: 1277 948 778
Fragments arranged by size:
1277 948 778
AccI GT'mk_AC
Cuts at: 0 2780 3003
Size: 2780 223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 341 1063 1709 1775 2638 3003
Size: 166 175 722 646 66 863 365
Fragments arranged by size:
863 722 646 365 175 166 66
AflIII A'CryG_T
Cuts at: 0 394 3003
Size: 394 2609
AleI CACnn'nnGTG
Cuts at: 0 1726 3003
Size: 1726 1277
AlwNI CAG_nnn'CTG
Cuts at: 0 364 1032 1407 1660 1702 2475 2490 3003
Size: 364 668 375 253 42 773 15 513
Fragments arranged by size:
773 668 513 375 364 253 42 15
ApaI G_GGCC'C
Cuts at: 0 50 297 1433 1560 1788 1795 3003
Size: 50 247 1136 127 228 7 1208
Fragments arranged by size:
1208 1136 247 228 127 50 7
AvaI C'yCGr_G
Cuts at: 0 689 1235 1802 2524 3003
Size: 689 546 567 722 479
Fragments arranged by size:
722 689 567 546 479
AvrII C'CTAG_G
Cuts at: 0 1863 3003
Size: 1863 1140
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 142 175 3003
Size: 142 33 2828
Fragments arranged by size:
2828 142 33
BanI G'GyrC_C
Cuts at: 0 369 508 1705 2233 2971 3003
Size: 369 139 1197 528 738 32
Fragments arranged by size:
1197 738 528 369 139 32
BanII G_rGCy'C
Cuts at: 0 43 50 104 297 662 782 949 1433
Size: 43 7 54 193 365 120 167 484
Cuts at: 1433 1560 1788 1795 1976 2573 2724 3003
Size: 127 228 7 181 597 151 279
Fragments arranged by size:
597 484 365 279 228 193 181 167
151 127 120 54 43 7 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 85 347 1222 1459 3003
Size: 85 262 875 237 1544
Fragments arranged by size:
1544 875 262 237 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2142 2176 3003
Size: 2142 34 827
Fragments arranged by size:
2142 827 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2605 3003
Size: 2605 398
Bme1580I G_kGCm'C
Cuts at: 0 50 297 1433 1560 1626 1788 1795 2950
Size: 50 247 1136 127 66 162 7 1155
Cuts at: 2950 2976 3003
Size: 26 27
Fragments arranged by size:
1155 1136 247 162 127 66 50 27
26 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 300 514 3003
Size: 300 214 2489
Fragments arranged by size:
2489 300 214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1990 2751 3003
Size: 1990 761 252
Fragments arranged by size:
1990 761 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 766 838 1078 1363 1679 2512 2587
Size: 556 210 72 240 285 316 833 75
Cuts at: 2587 2650 2788 3003
Size: 63 138 215
Fragments arranged by size:
833 556 316 285 240 215 210 138
75 72 63
Bpu10I CC'TnA_GC
Cuts at: 0 42 677 2087 2723 3003
Size: 42 635 1410 636 280
Fragments arranged by size:
1410 636 635 280 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1093 1878 1939 1960 2434 2698 3003
Size: 1093 785 61 21 474 264 305
Fragments arranged by size:
1093 785 474 305 264 61 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 799 1966 3003
Size: 434 365 1167 1037
Fragments arranged by size:
1167 1037 434 365
BsaAI yAC'GTr
Cuts at: 0 2637 3003
Size: 2637 366
BsaHI Gr'CG_yC
Cuts at: 0 1499 1616 3003
Size: 1499 117 1387
Fragments arranged by size:
1499 1387 117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 148 262 398 538 939 1150 1657
Size: 88 60 114 136 140 401 211 507
Cuts at: 1657 1753 1942 2146 2215 2263 2266 2428 2509
Size: 96 189 204 69 48 3 162 81
Cuts at: 2509 2824 3003
Size: 315 179
Fragments arranged by size:
507 401 315 211 204 189 179 162
140 136 114 96 88 81 69 60
48 3
BseYI C'CCAG_C
Cuts at: 0 296 696 1220 1302 1502 1626 1858 2229
Size: 296 400 524 82 200 124 232 371
Cuts at: 2229 2468 2600 3003
Size: 239 132 403
Fragments arranged by size:
524 403 400 371 296 239 232 200
132 124 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2080 2503 2644 2659 3003
Size: 2080 423 141 15 344
Fragments arranged by size:
2080 423 344 141 15
BsiHKAI G_wGCw'C
Cuts at: 0 782 949 1286 1976 2573 2636 3003
Size: 782 167 337 690 597 63 367
Fragments arranged by size:
782 690 597 367 337 167 63
BsmI GAATG_Cn'
Cuts at: 0 1011 2186 3003
Size: 1011 1175 817
Fragments arranged by size:
1175 1011 817
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1144 3003
Size: 1144 1859
Bsp1286I G_dGCh'C
Cuts at: 0 43 50 104 297 662 782 949 1286
Size: 43 7 54 193 365 120 167 337
Cuts at: 1286 1433 1560 1626 1788 1795 1976 2573 2636
Size: 147 127 66 162 7 181 597 63
Cuts at: 2636 2724 2950 2976 3003
Size: 88 226 26 27
Fragments arranged by size:
597 365 337 226 193 181 167 162
147 127 120 88 66 63 54 43
27 26 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1277 2225 2427 2848 3003
Size: 1277 948 202 421 155
Fragments arranged by size:
1277 948 421 202 155
BsrBI CCG'CTC
Cuts at: 0 183 1888 2376 3003
Size: 183 1705 488 627
Fragments arranged by size:
1705 627 488 183
BsrFI r'CCGG_y
Cuts at: 0 28 480 710 3003
Size: 28 452 230 2293
Fragments arranged by size:
2293 452 230 28
BsrGI T'GTAC_A
Cuts at: 0 638 2024 3003
Size: 638 1386 979
Fragments arranged by size:
1386 979 638
BssSI C'ACGA_G
Cuts at: 0 578 2690 3003
Size: 578 2112 313
Fragments arranged by size:
2112 578 313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 508 3003
Size: 508 2495
BstXI CCAn_nnnn'nTGG
Cuts at: 0 816 1567 3003
Size: 816 751 1436
Fragments arranged by size:
1436 816 751
BstYI r'GATC_y
Cuts at: 0 2657 3003
Size: 2657 346
Bsu36I CC'TnA_GG
Cuts at: 0 365 951 1133 1817 3003
Size: 365 586 182 684 1186
Fragments arranged by size:
1186 684 586 365 182
BtgI C'CryG_G
Cuts at: 0 128 3003
Size: 128 2875
BtsI GCAGTG_nn'
Cuts at: 0 525 853 1186 3003
Size: 525 328 333 1817
Fragments arranged by size:
1817 525 333 328
DraIII CAC_nnn'GTG
Cuts at: 0 508 3003
Size: 508 2495
EaeI y'GGCC_r
Cuts at: 0 728 1034 1250 1412 2606 3003
Size: 728 306 216 162 1194 397
Fragments arranged by size:
1194 728 397 306 216 162
EarI CTCTTCn'nnn_
Cuts at: 0 129 1751 3003
Size: 129 1622 1252
Fragments arranged by size:
1622 1252 129
EcoICRI GAG'CTC
Cuts at: 0 780 947 1974 2571 3003
Size: 780 167 1027 597 432
Fragments arranged by size:
1027 780 597 432 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 341 556 766 838 1063 1078 1363
Size: 166 175 215 210 72 225 15 285
Cuts at: 1363 1679 1709 1775 2512 2587 2638 2650 2788
Size: 316 30 66 737 75 51 12 138
Cuts at: 2788 3003
Size: 215
Fragments arranged by size:
737 316 285 225 215 215 210 175
166 138 75 72 66 51 30 15
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 667 901 1132 2806 3003
Size: 667 234 231 1674 197
Fragments arranged by size:
1674 667 234 231 197
EcoO109I rG'GnC_Cy
Cuts at: 0 46 47 377 1239 1429 1657 1784 1791
Size: 46 1 330 862 190 228 127 7
Cuts at: 1791 1792 1855 1867 2439 2613 3003
Size: 1 63 12 572 174 390
Fragments arranged by size:
862 572 390 330 228 190 174 127
63 46 12 7 1 1
FspI TGC'GCA
Cuts at: 0 1387 2554 3003
Size: 1387 1167 449
Fragments arranged by size:
1387 1167 449
HaeII r_GCGC'y
Cuts at: 0 364 612 1107 2038 3003
Size: 364 248 495 931 965
Fragments arranged by size:
965 931 495 364 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 1449 1481 3003
Size: 25 32 1392 32 1522
Fragments arranged by size:
1522 1392 32 32 25
HincII GTy'rAC
Cuts at: 0 2837 3003
Size: 2837 166
HindIII A'AGCT_T
Cuts at: 0 1519 3003
Size: 1519 1484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 3003
Size: 218 2785
MscI TGG'CCA
Cuts at: 0 730 1036 1252 3003
Size: 730 306 216 1751
Fragments arranged by size:
1751 730 306 216
MslI CAynn'nnrTG
Cuts at: 0 1726 3003
Size: 1726 1277
MspA1I CmG'CkG
Cuts at: 0 300 459 571 738 1341 2049 2074 2472
Size: 300 159 112 167 603 708 25 398
Cuts at: 2472 2604 2628 3003
Size: 132 24 375
Fragments arranged by size:
708 603 398 375 300 167 159 132
112 25 24
NaeI GCC'GGC
Cuts at: 0 30 482 3003
Size: 30 452 2521
Fragments arranged by size:
2521 452 30
NgoMIV G'CCGG_C
Cuts at: 0 28 480 3003
Size: 28 452 2523
Fragments arranged by size:
2523 452 28
NspI r_CATG'y
Cuts at: 0 398 987 1170 2304 3003
Size: 398 589 183 1134 699
Fragments arranged by size:
1134 699 589 398 183
PasI CC'CwG_GG
Cuts at: 0 1633 1780 1788 3003
Size: 1633 147 8 1215
Fragments arranged by size:
1633 1215 147 8
PciI A'CATG_T
Cuts at: 0 394 3003
Size: 394 2609
PflMI CCAn_nnn'nTGG
Cuts at: 0 1032 1702 2565 3003
Size: 1032 670 863 438
Fragments arranged by size:
1032 863 670 438
PfoI T'CCnGG_A
Cuts at: 0 782 2120 2804 3003
Size: 782 1338 684 199
Fragments arranged by size:
1338 782 684 199
PmlI CAC'GTG
Cuts at: 0 2637 3003
Size: 2637 366
PpuMI rG'GwC_Cy
Cuts at: 0 377 1855 1867 2439 2613 3003
Size: 377 1478 12 572 174 390
Fragments arranged by size:
1478 572 390 377 174 12
PspOMI G'GGCC_C
Cuts at: 0 46 293 1429 1556 1784 1791 3003
Size: 46 247 1136 127 228 7 1212
Fragments arranged by size:
1212 1136 247 228 127 46 7
PstI C_TGCA'G
Cuts at: 0 722 743 1070 1175 1313 2222 3003
Size: 722 21 327 105 138 909 781
Fragments arranged by size:
909 781 722 327 138 105 21
PvuII CAG'CTG
Cuts at: 0 300 459 571 738 1341 2472 2604 2628
Size: 300 159 112 167 603 1131 132 24
Cuts at: 2628 3003
Size: 375
Fragments arranged by size:
1131 603 375 300 167 159 132 112
24
SacI G_AGCT'C
Cuts at: 0 782 949 1976 2573 3003
Size: 782 167 1027 597 430
Fragments arranged by size:
1027 782 597 430 167
SanDI GG'GwC_CC
Cuts at: 0 377 3003
Size: 377 2626
ScaI AGT'ACT
Cuts at: 0 772 3003
Size: 772 2231
SfcI C'TryA_G
Cuts at: 0 154 604 718 739 1066 1171 1309 1877
Size: 154 450 114 21 327 105 138 568
Cuts at: 1877 2218 2326 2588 3003
Size: 341 108 262 415
Fragments arranged by size:
568 450 415 341 327 262 154 138
114 108 105 21
SgrAI Cr'CCGG_yG
Cuts at: 0 28 3003
Size: 28 2975
SmaI CCC'GGG
Cuts at: 0 1804 3003
Size: 1804 1199
SmlI C'TyrA_G
Cuts at: 0 1108 1893 1954 1975 2449 2713 3003
Size: 1108 785 61 21 474 264 290
Fragments arranged by size:
1108 785 474 290 264 61 21
SphI G_CATG'C
Cuts at: 0 987 1170 3003
Size: 987 183 1833
Fragments arranged by size:
1833 987 183
StuI AGG'CCT
Cuts at: 0 592 907 3003
Size: 592 315 2096
Fragments arranged by size:
2096 592 315
StyI C'CwwG_G
Cuts at: 0 485 563 809 1863 1916 2039 2442 3003
Size: 485 78 246 1054 53 123 403 561
Fragments arranged by size:
1054 561 485 403 246 123 78 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 496 1616 2221 3003
Size: 496 1120 605 782
Fragments arranged by size:
1120 782 605 496
TatI w'GTAC_w
Cuts at: 0 638 770 1530 2024 2822 3003
Size: 638 132 760 494 798 181
Fragments arranged by size:
798 760 638 494 181 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 832 864 3003
Size: 832 32 2139
Fragments arranged by size:
2139 832 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 1044 1590 3003
Size: 135 909 546 1413
Fragments arranged by size:
1413 909 546 135
XmaI C'CCGG_G
Cuts at: 0 1802 3003
Size: 1802 1201
XmnI GAAnn'nnTTC
Cuts at: 0 320 3003
Size: 320 2683
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI AvaI
AvrII BaeI BanI BanII BbsI BcgI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII HindIII MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI
ScaI SfcI SgrAI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI BamHI BbeI
BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BsaBI BsaWI BsaXI BsiEI BsiWI BspEI BspHI BsrDI
BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgfI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI ZraI