Restriction map for
pF1KSDA0662
(Linear) (Six-base) MAPSORT of: KIAA0662.seq Check: 5667 from: 1 to: 3303
KIAA0662.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1136 3303
Size: 1136 2167
AatII G_ACGT'C
Cuts at: 0 236 446 3303
Size: 236 210 2857
Fragments arranged by size:
2857 236 210
AccI GT'mk_AC
Cuts at: 0 896 2561 3303
Size: 896 1665 742
Fragments arranged by size:
1665 896 742
Acc65I G'GTAC_C
Cuts at: 0 773 3303
Size: 773 2530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 541 1582 1861 2557 3303
Size: 323 218 1041 279 696 746
Fragments arranged by size:
1041 746 696 323 279 218
AfeI AGC'GCT
Cuts at: 0 847 3303
Size: 847 2456
AflIII A'CryG_T
Cuts at: 0 2036 3303
Size: 2036 1267
AleI CACnn'nnGTG
Cuts at: 0 976 3303
Size: 976 2327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2548 3303
Size: 2548 755
AlwNI CAG_nnn'CTG
Cuts at: 0 3100 3303
Size: 3100 203
ApaI G_GGCC'C
Cuts at: 0 3120 3303
Size: 3120 183
ApoI r'AATT_y
Cuts at: 0 1199 1769 3266 3275 3303
Size: 1199 570 1497 9 28
Fragments arranged by size:
1497 1199 570 28 9
AvaI C'yCGr_G
Cuts at: 0 92 216 583 1601 3303
Size: 92 124 367 1018 1702
Fragments arranged by size:
1702 1018 367 124 92
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2762 2795 3303
Size: 2762 33 508
Fragments arranged by size:
2762 508 33
BanI G'GyrC_C
Cuts at: 0 9 123 760 773 2485 2739 2923 3103
Size: 9 114 637 13 1712 254 184 180
Cuts at: 3103 3303
Size: 200
Fragments arranged by size:
1712 637 254 200 184 180 114 13
9
BanII G_rGCy'C
Cuts at: 0 377 1265 1415 1966 2057 2278 2470 3050
Size: 377 888 150 551 91 221 192 580
Cuts at: 3050 3120 3303
Size: 70 183
Fragments arranged by size:
888 580 551 377 221 192 183 150
91 70
BbeI G_GCGC'C
Cuts at: 0 127 764 3303
Size: 127 637 2539
Fragments arranged by size:
2539 637 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 311 802 2657 3303
Size: 311 491 1855 646
Fragments arranged by size:
1855 646 491 311
BfrBI ATG'CAT
Cuts at: 0 676 3303
Size: 676 2627
BglI GCCn_nnn'nGGC
Cuts at: 0 1545 3303
Size: 1545 1758
Bme1580I G_kGCm'C
Cuts at: 0 14 1724 2490 3120 3303
Size: 14 1710 766 630 183
Fragments arranged by size:
1710 766 630 183 14
BmgBI CAC'GTC
Cuts at: 0 309 3303
Size: 309 2994
BmrI ACTGGGnnnn_n'
Cuts at: 0 1078 3303
Size: 1078 2225
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1350 1629 3054 3303
Size: 1350 279 1425 249
Fragments arranged by size:
1425 1350 279 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1474 2350 2659 3303
Size: 1153 321 876 309 644
Fragments arranged by size:
1153 876 644 321 309
Bpu10I CC'TnA_GC
Cuts at: 0 1535 3303
Size: 1535 1768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 2041 3303
Size: 769 1272 1262
Fragments arranged by size:
1272 1262 769
BsaI GGTCTCn'nnnn_
Cuts at: 0 2785 3303
Size: 2785 518
BsaAI yAC'GTr
Cuts at: 0 456 780 2037 2441 3303
Size: 456 324 1257 404 862
Fragments arranged by size:
1257 862 456 404 324
BsaHI Gr'CG_yC
Cuts at: 0 124 233 443 761 3303
Size: 124 109 210 318 2542
Fragments arranged by size:
2542 318 210 124 109
BsaWI w'CCGG_w
Cuts at: 0 1350 3303
Size: 1350 1953
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 992 1022 1402 1432 1447 1477 2875 2905
Size: 992 30 380 30 15 30 1398 30
Cuts at: 2905 3303
Size: 398
Fragments arranged by size:
1398 992 398 380 30 30 30 30
15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 1423 1867 1921 2187 2329 2848 3303
Size: 370 1053 444 54 266 142 519 455
Fragments arranged by size:
1053 519 455 444 370 266 142 54
BseYI C'CCAG_C
Cuts at: 0 342 402 2341 2365 2825 3137 3303
Size: 342 60 1939 24 460 312 166
Fragments arranged by size:
1939 460 342 312 166 60 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 2200 2540 3303
Size: 265 1935 340 763
Fragments arranged by size:
1935 763 340 265
BsiEI CG_ry'CG
Cuts at: 0 1222 1351 2244 2677 3245 3303
Size: 1222 129 893 433 568 58
Fragments arranged by size:
1222 893 568 433 129 58
BsiHKAI G_wGCw'C
Cuts at: 0 362 785 1265 1274 2057 2278 2470 3303
Size: 362 423 480 9 783 221 192 833
Fragments arranged by size:
833 783 480 423 362 221 192 9
BsiWI C'GTAC_G
Cuts at: 0 3223 3303
Size: 3223 80
BsmI GAATG_Cn'
Cuts at: 0 1138 1335 2396 3303
Size: 1138 197 1061 907
Fragments arranged by size:
1138 1061 907 197
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1424 3194 3303
Size: 1424 1770 109
Fragments arranged by size:
1770 1424 109
Bsp1286I G_dGCh'C
Cuts at: 0 14 362 377 785 1265 1274 1415 1724
Size: 14 348 15 408 480 9 141 309
Cuts at: 1724 1966 2057 2278 2470 2490 3050 3120 3303
Size: 242 91 221 192 20 560 70 183
Fragments arranged by size:
560 480 408 348 309 242 221 192
183 141 91 70 20 15 14 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1136 1646 2306 2417 3303
Size: 1136 510 660 111 886
Fragments arranged by size:
1136 886 660 510 111
BsrDI GCAATG_nn'
Cuts at: 0 676 2253 3303
Size: 676 1577 1050
Fragments arranged by size:
1577 1050 676
BsrFI r'CCGG_y
Cuts at: 0 29 816 2920 2976 3303
Size: 29 787 2104 56 327
Fragments arranged by size:
2104 787 327 56 29
BssHII G'CGCG_C
Cuts at: 0 2324 3303
Size: 2324 979
BstXI CCAn_nnnn'nTGG
Cuts at: 0 615 3303
Size: 615 2688
BstYI r'GATC_y
Cuts at: 0 956 1313 3303
Size: 956 357 1990
Fragments arranged by size:
1990 956 357
Bsu36I CC'TnA_GG
Cuts at: 0 948 3303
Size: 948 2355
BtgI C'CryG_G
Cuts at: 0 168 2399 2481 3143 3303
Size: 168 2231 82 662 160
Fragments arranged by size:
2231 662 168 160 82
BtsI GCAGTG_nn'
Cuts at: 0 144 2514 3303
Size: 144 2370 789
Fragments arranged by size:
2370 789 144
EaeI y'GGCC_r
Cuts at: 0 664 683 824 1304 2241 2402 2451 3008
Size: 664 19 141 480 937 161 49 557
Cuts at: 3008 3126 3303
Size: 118 177
Fragments arranged by size:
937 664 557 480 177 161 141 118
49 19
EagI C'GGCC_G
Cuts at: 0 2241 3303
Size: 2241 1062
EarI CTCTTCn'nnn_
Cuts at: 0 268 943 1844 2533 2576 3303
Size: 268 675 901 689 43 727
Fragments arranged by size:
901 727 689 675 268 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3094 3303
Size: 3094 209
EcoICRI GAG'CTC
Cuts at: 0 1263 2055 2276 2468 3303
Size: 1263 792 221 192 835
Fragments arranged by size:
1263 835 792 221 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 541 1153 1474 1582 1861 2350 2557
Size: 323 218 612 321 108 279 489 207
Cuts at: 2557 2659 3303
Size: 102 644
Fragments arranged by size:
644 612 489 323 321 279 218 207
108 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 294 1132 2302 3303
Size: 294 838 1170 1001
Fragments arranged by size:
1170 1001 838 294
EcoO109I rG'GnC_Cy
Cuts at: 0 564 1547 1952 1979 3116 3303
Size: 564 983 405 27 1137 187
Fragments arranged by size:
1137 983 564 405 187 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 3303
Size: 198 3105
FseI GG_CCGG'CC
Cuts at: 0 820 3303
Size: 820 2483
HaeII r_GCGC'y
Cuts at: 0 127 229 764 849 3303
Size: 127 102 535 85 2454
Fragments arranged by size:
2454 535 127 102 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 789 821 992 1024 1402 1434 1447 1479
Size: 789 32 171 32 378 32 13 32
Cuts at: 1479 2023 2055 2182 2214 3303
Size: 544 32 127 32 1089
Fragments arranged by size:
1089 789 544 378 171 127 32 32
32 32 32 32 13
HincII GTy'rAC
Cuts at: 0 897 2562 3303
Size: 897 1665 741
Fragments arranged by size:
1665 897 741
KasI G'GCGC_C
Cuts at: 0 123 760 3303
Size: 123 637 2543
Fragments arranged by size:
2543 637 123
KpnI G_GTAC'C
Cuts at: 0 777 3303
Size: 777 2526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1371 1943 2021 2102 2444 3303
Size: 1371 572 78 81 342 859
Fragments arranged by size:
1371 859 572 342 81 78
MscI TGG'CCA
Cuts at: 0 666 685 1306 2404 2453 3303
Size: 666 19 621 1098 49 850
Fragments arranged by size:
1098 850 666 621 49 19
MslI CAynn'nnrTG
Cuts at: 0 601 976 2597 3303
Size: 601 375 1621 706
Fragments arranged by size:
1621 706 601 375
MspA1I CmG'CkG
Cuts at: 0 105 1170 2284 3100 3208 3303
Size: 105 1065 1114 816 108 95
Fragments arranged by size:
1114 1065 816 108 105 95
NaeI GCC'GGC
Cuts at: 0 31 818 2922 3303
Size: 31 787 2104 381
Fragments arranged by size:
2104 787 381 31
NarI GG'CG_CC
Cuts at: 0 124 761 3303
Size: 124 637 2542
Fragments arranged by size:
2542 637 124
NcoI C'CATG_G
Cuts at: 0 168 2399 2481 3143 3303
Size: 168 2231 82 662 160
Fragments arranged by size:
2231 662 168 160 82
NdeI CA'TA_TG
Cuts at: 0 1044 3303
Size: 1044 2259
NgoMIV G'CCGG_C
Cuts at: 0 29 816 2920 3303
Size: 29 787 2104 383
Fragments arranged by size:
2104 787 383 29
NsiI A_TGCA'T
Cuts at: 0 678 3303
Size: 678 2625
NspI r_CATG'y
Cuts at: 0 1746 3303
Size: 1746 1557
PasI CC'CwG_GG
Cuts at: 0 296 3151 3303
Size: 296 2855 152
Fragments arranged by size:
2855 296 152
PflMI CCAn_nnn'nTGG
Cuts at: 0 3144 3303
Size: 3144 159
PfoI T'CCnGG_A
Cuts at: 0 1265 1451 3303
Size: 1265 186 1852
Fragments arranged by size:
1852 1265 186
PmlI CAC'GTG
Cuts at: 0 780 2441 3303
Size: 780 1661 862
Fragments arranged by size:
1661 862 780
PpuMI rG'GwC_Cy
Cuts at: 0 564 3303
Size: 564 2739
PshAI GACnn'nnGTC
Cuts at: 0 487 3303
Size: 487 2816
PspOMI G'GGCC_C
Cuts at: 0 3116 3303
Size: 3116 187
PspXI vC'TCGA_Gb
Cuts at: 0 583 3303
Size: 583 2720
PstI C_TGCA'G
Cuts at: 0 2306 2414 2435 2814 3303
Size: 2306 108 21 379 489
Fragments arranged by size:
2306 489 379 108 21
PvuI CG_AT'CG
Cuts at: 0 1222 3303
Size: 1222 2081
PvuII CAG'CTG
Cuts at: 0 1170 2284 3303
Size: 1170 1114 1019
Fragments arranged by size:
1170 1114 1019
RsrII CG'GwC_CG
Cuts at: 0 85 3303
Size: 85 3218
SacI G_AGCT'C
Cuts at: 0 1265 2057 2278 2470 3303
Size: 1265 792 221 192 833
Fragments arranged by size:
1265 833 792 221 192
SalI G'TCGA_C
Cuts at: 0 895 2560 3303
Size: 895 1665 743
Fragments arranged by size:
1665 895 743
SapI GCTCTTCn'nnn_
Cuts at: 0 268 2576 3303
Size: 268 2308 727
Fragments arranged by size:
2308 727 268
SbfI CC_TGCA'GG
Cuts at: 0 2414 3303
Size: 2414 889
ScaI AGT'ACT
Cuts at: 0 706 3303
Size: 706 2597
SexAI A'CCwGG_T
Cuts at: 0 890 3303
Size: 890 2413
SfcI C'TryA_G
Cuts at: 0 543 2302 2410 2431 2709 2810 3303
Size: 543 1759 108 21 278 101 493
Fragments arranged by size:
1759 543 493 278 108 101 21
SfoI GGC'GCC
Cuts at: 0 125 762 3303
Size: 125 637 2541
Fragments arranged by size:
2541 637 125
SmaI CCC'GGG
Cuts at: 0 94 218 1603 3303
Size: 94 124 1385 1700
Fragments arranged by size:
1700 1385 124 94
SmlI C'TyrA_G
Cuts at: 0 583 784 2056 3303
Size: 583 201 1272 1247
Fragments arranged by size:
1272 1247 583 201
StuI AGG'CCT
Cuts at: 0 2416 2757 3022 3303
Size: 2416 341 265 281
Fragments arranged by size:
2416 341 281 265
StyI C'CwwG_G
Cuts at: 0 168 1188 1543 1655 1940 2399 2459 2481
Size: 168 1020 355 112 285 459 60 22
Cuts at: 2481 3143 3303
Size: 662 160
Fragments arranged by size:
1020 662 459 355 285 168 160 112
60 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 102 1632 3303
Size: 102 1530 1671
Fragments arranged by size:
1671 1530 102
TatI w'GTAC_w
Cuts at: 0 17 704 1811 2333 3089 3303
Size: 17 687 1107 522 756 214
Fragments arranged by size:
1107 756 687 522 214 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1607 1639 3303
Size: 1607 32 1664
Fragments arranged by size:
1664 1607 32
Tth111I GACn'n_nGTC
Cuts at: 0 892 3303
Size: 892 2411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 751 1629 1731 3303
Size: 751 878 102 1572
Fragments arranged by size:
1572 878 751 102
XhoI C'TCGA_G
Cuts at: 0 583 3303
Size: 583 2720
XmaI C'CCGG_G
Cuts at: 0 92 216 1601 3303
Size: 92 124 1385 1702
Fragments arranged by size:
1702 1385 124 92
XmnI GAAnn'nnTTC
Cuts at: 0 799 2381 3303
Size: 799 1582 922
Fragments arranged by size:
1582 922 799
ZraI GAC'GTC
Cuts at: 0 234 444 3303
Size: 234 210 2859
Fragments arranged by size:
2859 234 210
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApoI AvaI BaeI BanI BanII
BbeI BbsI BfrBI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BssHII BstXI BstYI Bsu36I
BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AloI ApaLI AscI AseI
AsiSI AvrII BamHI BbvCI BcgI BciVI BclI BglII
BlpI BmtI BsaBI BspEI BspHI BsrBI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EcoRI EcoRV FspI FspAI HindIII HpaI
MfeI MluI NheI NotI NruI PacI PciI PmeI
PpiI PsiI PsrI SacII SanDI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI