Restriction map for
pF1KSDA0663
(Linear) (Six-base) MAPSORT of: KIAA0663.seq Check: 7842 from: 1 to: 2430
KIAA0663.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 692 1160 1552 1610 2171 2186 2430
Size: 578 114 468 392 58 561 15 244
Fragments arranged by size:
578 561 468 392 244 114 58 15
AflII C'TTAA_G
Cuts at: 0 991 2430
Size: 991 1439
AflIII A'CryG_T
Cuts at: 0 43 2430
Size: 43 2387
AleI CACnn'nnGTG
Cuts at: 0 1798 2430
Size: 1798 632
AlwNI CAG_nnn'CTG
Cuts at: 0 754 2143 2430
Size: 754 1389 287
Fragments arranged by size:
1389 754 287
ApaLI G'TGCA_C
Cuts at: 0 1405 2430
Size: 1405 1025
ApoI r'AATT_y
Cuts at: 0 188 630 862 1088 1658 2430
Size: 188 442 232 226 570 772
Fragments arranged by size:
772 570 442 232 226 188
AseI AT'TA_AT
Cuts at: 0 470 2430
Size: 470 1960
BamHI G'GATC_C
Cuts at: 0 2373 2430
Size: 2373 57
BanI G'GyrC_C
Cuts at: 0 1817 2430
Size: 1817 613
BanII G_rGCy'C
Cuts at: 0 743 1465 2430
Size: 743 722 965
Fragments arranged by size:
965 743 722
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 1081 2430
Size: 23 1058 1349
Fragments arranged by size:
1349 1058 23
BbvCI CC'TCA_GC
Cuts at: 0 398 2430
Size: 398 2032
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 128 162 2430
Size: 128 34 2268
Fragments arranged by size:
2268 128 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1275 2430
Size: 1275 1155
BclI T'GATC_A
Cuts at: 0 501 2430
Size: 501 1929
BglI GCCn_nnn'nGGC
Cuts at: 0 2095 2430
Size: 2095 335
Bme1580I G_kGCm'C
Cuts at: 0 1409 2430
Size: 1409 1021
BmrI ACTGGGnnnn_n'
Cuts at: 0 859 2187 2430
Size: 859 1328 243
Fragments arranged by size:
1328 859 243
BmtI G_CTAG'C
Cuts at: 0 347 2430
Size: 347 2083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1456 1490 2046 2430
Size: 923 533 34 556 384
Fragments arranged by size:
923 556 533 384 34
Bpu10I CC'TnA_GC
Cuts at: 0 398 737 2430
Size: 398 339 1693
Fragments arranged by size:
1693 398 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 959 1123 2425 2430
Size: 670 289 164 1302 5
Fragments arranged by size:
1302 670 289 164 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 1627 1672 2430
Size: 1627 45 758
Fragments arranged by size:
1627 758 45
BsaBI GATnn'nnATC
Cuts at: 0 500 2430
Size: 500 1930
BsaWI w'CCGG_w
Cuts at: 0 590 2430
Size: 590 1840
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1182 1384 2430
Size: 1182 202 1046
Fragments arranged by size:
1182 1046 202
BseYI C'CCAG_C
Cuts at: 0 2085 2430
Size: 2085 345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1474 1928 2430
Size: 168 1306 454 502
Fragments arranged by size:
1306 502 454 168
BsiHKAI G_wGCw'C
Cuts at: 0 1409 1465 1793 2430
Size: 1409 56 328 637
Fragments arranged by size:
1409 637 328 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1493 2430
Size: 1493 937
Bsp1286I G_dGCh'C
Cuts at: 0 743 1409 1465 1793 2430
Size: 743 666 56 328 637
Fragments arranged by size:
743 666 637 328 56
BspEI T'CCGG_A
Cuts at: 0 590 2430
Size: 590 1840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 606 1369 2430
Size: 140 466 763 1061
Fragments arranged by size:
1061 763 466 140
BsrDI GCAATG_nn'
Cuts at: 0 2036 2430
Size: 2036 394
BssSI C'ACGA_G
Cuts at: 0 1495 2430
Size: 1495 935
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1404 2430
Size: 1404 1026
BstYI r'GATC_y
Cuts at: 0 1064 2373 2430
Size: 1064 1309 57
Fragments arranged by size:
1309 1064 57
Bsu36I CC'TnA_GG
Cuts at: 0 1872 2430
Size: 1872 558
BtsI GCAGTG_nn'
Cuts at: 0 189 1793 2021 2073 2430
Size: 189 1604 228 52 357
Fragments arranged by size:
1604 357 228 189 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1626 1661 2430
Size: 1626 35 769
Fragments arranged by size:
1626 769 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2137 2430
Size: 2137 293
EarI CTCTTCn'nnn_
Cuts at: 0 323 1550 1745 2205 2430
Size: 323 1227 195 460 225
Fragments arranged by size:
1227 460 323 225 195
EcoICRI GAG'CTC
Cuts at: 0 1463 2430
Size: 1463 967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 692 923 1160 1456 1490 1552 1610
Size: 578 114 231 237 296 34 62 58
Cuts at: 1610 2046 2171 2186 2430
Size: 436 125 15 244
Fragments arranged by size:
578 436 296 244 237 231 125 114
62 58 34 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 8 2430
Size: 8 2422
EcoO109I rG'GnC_Cy
Cuts at: 0 751 1150 1224 2430
Size: 751 399 74 1206
Fragments arranged by size:
1206 751 399 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1108 2413 2430
Size: 1108 1305 17
Fragments arranged by size:
1305 1108 17
HaeII r_GCGC'y
Cuts at: 0 132 2430
Size: 132 2298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 777 809 1634 1666 1741 1773 2119 2151
Size: 777 32 825 32 75 32 346 32
Cuts at: 2151 2430
Size: 279
Fragments arranged by size:
825 777 346 279 75 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1647 2430
Size: 1647 783
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2212 2430
Size: 2212 218
MslI CAynn'nnrTG
Cuts at: 0 1798 2430
Size: 1798 632
MspA1I CmG'CkG
Cuts at: 0 62 402 1015 2044 2101 2430
Size: 62 340 613 1029 57 329
Fragments arranged by size:
1029 613 340 329 62 57
NheI G'CTAG_C
Cuts at: 0 343 2430
Size: 343 2087
NspI r_CATG'y
Cuts at: 0 47 1011 1701 2430
Size: 47 964 690 729
Fragments arranged by size:
964 729 690 47
PciI A'CATG_T
Cuts at: 0 43 2430
Size: 43 2387
PflMI CCAn_nnn'nTGG
Cuts at: 0 1857 2430
Size: 1857 573
PpuMI rG'GwC_Cy
Cuts at: 0 751 1150 1224 2430
Size: 751 399 74 1206
Fragments arranged by size:
1206 751 399 74
PshAI GACnn'nnGTC
Cuts at: 0 586 2430
Size: 586 1844
PstI C_TGCA'G
Cuts at: 0 480 603 1380 2430
Size: 480 123 777 1050
Fragments arranged by size:
1050 777 480 123
PvuII CAG'CTG
Cuts at: 0 62 402 1015 2101 2430
Size: 62 340 613 1086 329
Fragments arranged by size:
1086 613 340 329 62
SacI G_AGCT'C
Cuts at: 0 1465 2430
Size: 1465 965
SbfI CC_TGCA'GG
Cuts at: 0 1380 2430
Size: 1380 1050
SfcI C'TryA_G
Cuts at: 0 476 599 1376 2074 2430
Size: 476 123 777 698 356
Fragments arranged by size:
777 698 476 356 123
SmlI C'TyrA_G
Cuts at: 0 649 974 991 1138 2404 2430
Size: 649 325 17 147 1266 26
Fragments arranged by size:
1266 649 325 147 26 17
StyI C'CwwG_G
Cuts at: 0 821 1919 2430
Size: 821 1098 511
Fragments arranged by size:
1098 821 511
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1356 2430
Size: 1356 1074
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 563 595 2430
Size: 563 32 1835
Fragments arranged by size:
1835 563 32
XmnI GAAnn'nnTTC
Cuts at: 0 437 864 1088 1091 2430
Size: 437 427 224 3 1339
Fragments arranged by size:
1339 437 427 224 3
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApaLI ApoI AseI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrDI BssSI BstAPI BstYI Bsu36I BtsI
CspCI DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII MmeI MslI MspA1I NheI NspI
PciI PflMI PpuMI PshAI PstI PvuII SacI SbfI
SfcI SmlI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI AscI AsiSI AvaI AvrII BaeI
BbeI BfrBI BglII BlpI BmgBI BplI BsaAI BsaHI
BsaXI BsiEI BsiWI BsmI BspHI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
DraI DraIII EaeI EagI EciI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI ZraI