Restriction map for
pF1KSDA0665
(Linear) (Six-base) MAPSORT of: KIAA0665.seq Check: 4434 from: 1 to: 2268
KIAA0665.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 702 1901 2268
Size: 702 1199 367
Fragments arranged by size:
1199 702 367
AatII G_ACGT'C
Cuts at: 0 479 2268
Size: 479 1789
AccI GT'mk_AC
Cuts at: 0 473 698 2268
Size: 473 225 1570
Fragments arranged by size:
1570 473 225
Acc65I G'GTAC_C
Cuts at: 0 1301 2268
Size: 1301 967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1543 1714 2029 2050 2268
Size: 1034 509 171 315 21 218
Fragments arranged by size:
1034 509 315 218 171 21
AgeI A'CCGG_T
Cuts at: 0 1447 2268
Size: 1447 821
AhdI GACnn_n'nnGTC
Cuts at: 0 150 1571 2268
Size: 150 1421 697
Fragments arranged by size:
1421 697 150
AleI CACnn'nnGTG
Cuts at: 0 991 2268
Size: 991 1277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1009 1966 2268
Size: 1009 957 302
Fragments arranged by size:
1009 957 302
AlwNI CAG_nnn'CTG
Cuts at: 0 1536 1650 1734 1860 2268
Size: 1536 114 84 126 408
Fragments arranged by size:
1536 408 126 114 84
ApaI G_GGCC'C
Cuts at: 0 50 106 322 1323 1372 2268
Size: 50 56 216 1001 49 896
Fragments arranged by size:
1001 896 216 56 50 49
ApaLI G'TGCA_C
Cuts at: 0 994 1492 2268
Size: 994 498 776
Fragments arranged by size:
994 776 498
AvaI C'yCGr_G
Cuts at: 0 27 156 178 225 253 315 385 532
Size: 27 129 22 47 28 62 70 147
Cuts at: 532 1354 1973 2147 2268
Size: 822 619 174 121
Fragments arranged by size:
822 619 174 147 129 121 70 62
47 28 27 22
BamHI G'GATC_C
Cuts at: 0 738 2268
Size: 738 1530
BanI G'GyrC_C
Cuts at: 0 1090 1301 1823 2083 2268
Size: 1090 211 522 260 185
Fragments arranged by size:
1090 522 260 211 185
BanII G_rGCy'C
Cuts at: 0 34 50 106 307 322 386 590 752
Size: 34 16 56 201 15 64 204 162
Cuts at: 752 860 937 1082 1323 1361 1372 1586 2159
Size: 108 77 145 241 38 11 214 573
Cuts at: 2159 2268
Size: 109
Fragments arranged by size:
573 241 214 204 201 162 145 109
108 77 64 56 38 34 16 15
11
BbeI G_GCGC'C
Cuts at: 0 2087 2268
Size: 2087 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 471 776 1392 2268
Size: 471 305 616 876
Fragments arranged by size:
876 616 471 305
BbvCI CC'TCA_GC
Cuts at: 0 559 2071 2268
Size: 559 1512 197
Fragments arranged by size:
1512 559 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1072 1106 1602 1636 2268
Size: 1072 34 496 34 632
Fragments arranged by size:
1072 632 496 34 34
BclI T'GATC_A
Cuts at: 0 758 2268
Size: 758 1510
BglI GCCn_nnn'nGGC
Cuts at: 0 16 45 66 75 220 2268
Size: 16 29 21 9 145 2048
Fragments arranged by size:
2048 145 29 21 16 9
Bme1580I G_kGCm'C
Cuts at: 0 50 106 322 998 1052 1095 1323 1372
Size: 50 56 216 676 54 43 228 49
Cuts at: 1372 1496 2268
Size: 124 772
Fragments arranged by size:
772 676 228 216 124 56 54 50
49 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 737 1256 2268
Size: 737 519 1012
Fragments arranged by size:
1012 737 519
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2154 2173 2268
Size: 2154 19 95
Fragments arranged by size:
2154 95 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 541 1266 1360 1441 1531 1735 1906
Size: 253 288 725 94 81 90 204 171
Cuts at: 1906 1930 2008 2268
Size: 24 78 260
Fragments arranged by size:
725 288 260 253 204 171 94 90
81 78 24
Bpu10I CC'TnA_GC
Cuts at: 0 559 1001 2071 2268
Size: 559 442 1070 197
Fragments arranged by size:
1070 559 442 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1888 2268
Size: 1888 380
BsaI GGTCTCn'nnnn_
Cuts at: 0 242 955 1204 1558 2233 2268
Size: 242 713 249 354 675 35
Fragments arranged by size:
713 675 354 249 242 35
BsaHI Gr'CG_yC
Cuts at: 0 4 311 476 1067 1572 2084 2268
Size: 4 307 165 591 505 512 184
Fragments arranged by size:
591 512 505 307 184 165 4
BsaWI w'CCGG_w
Cuts at: 0 1447 1673 2268
Size: 1447 226 595
Fragments arranged by size:
1447 595 226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 116 146 919 949 1573 1603 2268
Size: 116 30 773 30 624 30 665
Fragments arranged by size:
773 665 624 116 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 403 868 1090 1174 1390 1528 1672
Size: 394 9 465 222 84 216 138 144
Cuts at: 1672 1732 1735 1806 1891 2059 2197 2268
Size: 60 3 71 85 168 138 71
Fragments arranged by size:
465 394 222 216 168 144 138 138
85 84 71 71 60 9 3
BseYI C'CCAG_C
Cuts at: 0 1230 2268
Size: 1230 1038
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1291 1665 2268
Size: 1291 374 603
Fragments arranged by size:
1291 603 374
BsiEI CG_ry'CG
Cuts at: 0 1046 2268
Size: 1046 1222
BsiHKAI G_wGCw'C
Cuts at: 0 946 998 1166 1361 1496 1586 1892 2159
Size: 946 52 168 195 135 90 306 267
Cuts at: 2159 2268
Size: 109
Fragments arranged by size:
946 306 267 195 168 135 109 90
52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2148 2268
Size: 2148 120
Bsp1286I G_dGCh'C
Cuts at: 0 34 50 106 307 322 386 590 752
Size: 34 16 56 201 15 64 204 162
Cuts at: 752 860 937 946 998 1052 1082 1095 1166
Size: 108 77 9 52 54 30 13 71
Cuts at: 1166 1323 1361 1372 1496 1586 1892 2159 2268
Size: 157 38 11 124 90 306 267 109
Fragments arranged by size:
306 267 204 201 162 157 124 109
108 90 77 71 64 56 54 52
38 34 30 16 15 13 11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 702 1313 1490 1637 1901 2268
Size: 702 611 177 147 264 367
Fragments arranged by size:
702 611 367 264 177 147
BsrDI GCAATG_nn'
Cuts at: 0 1382 2268
Size: 1382 886
BsrFI r'CCGG_y
Cuts at: 0 15 219 1033 1447 2268
Size: 15 204 814 414 821
Fragments arranged by size:
821 814 414 204 15
BssHII G'CGCG_C
Cuts at: 0 419 2268
Size: 419 1849
BssSI C'ACGA_G
Cuts at: 0 1816 2268
Size: 1816 452
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1030 1491 2268
Size: 1030 461 777
Fragments arranged by size:
1030 777 461
BstXI CCAn_nnnn'nTGG
Cuts at: 0 990 2268
Size: 990 1278
BstYI r'GATC_y
Cuts at: 0 738 807 2268
Size: 738 69 1461
Fragments arranged by size:
1461 738 69
Bsu36I CC'TnA_GG
Cuts at: 0 1468 2268
Size: 1468 800
BtgI C'CryG_G
Cuts at: 0 286 500 590 1070 1086 1125 1328 2268
Size: 286 214 90 480 16 39 203 940
Fragments arranged by size:
940 480 286 214 203 90 39 16
BtsI GCAGTG_nn'
Cuts at: 0 1194 1245 2268
Size: 1194 51 1023
Fragments arranged by size:
1194 1023 51
DrdI GACnn_nn'nnGTC
Cuts at: 0 661 1396 2247 2268
Size: 661 735 851 21
Fragments arranged by size:
851 735 661 21
EaeI y'GGCC_r
Cuts at: 0 816 1043 1128 2228 2268
Size: 816 227 85 1100 40
Fragments arranged by size:
1100 816 227 85 40
EagI C'GGCC_G
Cuts at: 0 1043 2268
Size: 1043 1225
EarI CTCTTCn'nnn_
Cuts at: 0 1556 1603 1769 2101 2268
Size: 1556 47 166 332 167
Fragments arranged by size:
1556 332 167 166 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 1210 2185 2268
Size: 252 958 975 83
Fragments arranged by size:
975 958 252 83
EcoICRI GAG'CTC
Cuts at: 0 1359 1584 2157 2268
Size: 1359 225 573 111
Fragments arranged by size:
1359 573 225 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 541 1034 1266 1360 1441 1531 1543
Size: 253 288 493 232 94 81 90 12
Cuts at: 1543 1714 1735 1906 1930 2008 2029 2050 2268
Size: 171 21 171 24 78 21 21 218
Fragments arranged by size:
493 288 253 232 218 171 171 94
90 81 78 24 21 21 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1471 2268
Size: 1471 797
EcoO109I rG'GnC_Cy
Cuts at: 0 46 103 529 536 1319 1320 1335 1344
Size: 46 57 426 7 783 1 15 9
Cuts at: 1344 1368 1869 2268
Size: 24 501 399
Fragments arranged by size:
783 501 426 399 57 46 24 15
9 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 361 1416 2112 2268
Size: 361 1055 696 156
Fragments arranged by size:
1055 696 361 156
FseI GG_CCGG'CC
Cuts at: 0 223 2268
Size: 223 2045
HaeII r_GCGC'y
Cuts at: 0 130 215 343 520 2087 2268
Size: 130 85 128 177 1567 181
Fragments arranged by size:
1567 181 177 130 128 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 136 148 168 466 498 1557 1589
Size: 116 20 12 20 298 32 1059 32
Cuts at: 1589 1735 1767 2268
Size: 146 32 501
Fragments arranged by size:
1059 501 298 146 116 32 32 32
20 20 12
HincII GTy'rAC
Cuts at: 0 474 2268
Size: 474 1794
KasI G'GCGC_C
Cuts at: 0 2083 2268
Size: 2083 185
KpnI G_GTAC'C
Cuts at: 0 1305 2268
Size: 1305 963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1291 1718 2268
Size: 187 1104 427 550
Fragments arranged by size:
1104 550 427 187
MscI TGG'CCA
Cuts at: 0 818 1130 2230 2268
Size: 818 312 1100 38
Fragments arranged by size:
1100 818 312 38
MslI CAynn'nnrTG
Cuts at: 0 991 1124 1144 2268
Size: 991 133 20 1124
Fragments arranged by size:
1124 991 133 20
MspA1I CmG'CkG
Cuts at: 0 187 288 1230 1488 1521 1884 1923 2268
Size: 187 101 942 258 33 363 39 345
Fragments arranged by size:
942 363 345 258 187 101 39 33
NaeI GCC'GGC
Cuts at: 0 17 221 1035 2268
Size: 17 204 814 1233
Fragments arranged by size:
1233 814 204 17
NarI GG'CG_CC
Cuts at: 0 2084 2268
Size: 2084 184
NcoI C'CATG_G
Cuts at: 0 1070 1086 1125 1328 2268
Size: 1070 16 39 203 940
Fragments arranged by size:
1070 940 203 39 16
NgoMIV G'CCGG_C
Cuts at: 0 15 219 1033 2268
Size: 15 204 814 1235
Fragments arranged by size:
1235 814 204 15
PasI CC'CwG_GG
Cuts at: 0 322 2268
Size: 322 1946
PfoI T'CCnGG_A
Cuts at: 0 370 1403 1556 2255 2268
Size: 370 1033 153 699 13
Fragments arranged by size:
1033 699 370 153 13
PpuMI rG'GwC_Cy
Cuts at: 0 529 536 1344 1869 2268
Size: 529 7 808 525 399
Fragments arranged by size:
808 529 525 399 7
PspOMI G'GGCC_C
Cuts at: 0 46 102 318 1319 1368 2268
Size: 46 56 216 1001 49 900
Fragments arranged by size:
1001 900 216 56 49 46
PstI C_TGCA'G
Cuts at: 0 192 1240 1487 1634 1898 1955 2130 2204
Size: 192 1048 247 147 264 57 175 74
Cuts at: 2204 2268
Size: 64
Fragments arranged by size:
1048 264 247 192 175 147 74 64
57
PvuII CAG'CTG
Cuts at: 0 1230 1488 1521 1884 2268
Size: 1230 258 33 363 384
Fragments arranged by size:
1230 384 363 258 33
SacI G_AGCT'C
Cuts at: 0 1361 1586 2159 2268
Size: 1361 225 573 109
Fragments arranged by size:
1361 573 225 109
SacII CC_GC'GG
Cuts at: 0 289 2268
Size: 289 1979
SalI G'TCGA_C
Cuts at: 0 472 2268
Size: 472 1796
SanDI GG'GwC_CC
Cuts at: 0 536 2268
Size: 536 1732
SapI GCTCTTCn'nnn_
Cuts at: 0 1603 1769 2268
Size: 1603 166 499
Fragments arranged by size:
1603 499 166
SbfI CC_TGCA'GG
Cuts at: 0 1955 2204 2268
Size: 1955 249 64
Fragments arranged by size:
1955 249 64
ScaI AGT'ACT
Cuts at: 0 733 2268
Size: 733 1535
SfcI C'TryA_G
Cuts at: 0 188 948 1236 1483 1630 1642 1894 1951
Size: 188 760 288 247 147 12 252 57
Cuts at: 1951 2126 2200 2268
Size: 175 74 68
Fragments arranged by size:
760 288 252 247 188 175 147 74
68 57 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 16 75 2268
Size: 16 59 2193
Fragments arranged by size:
2193 59 16
SfoI GGC'GCC
Cuts at: 0 2085 2268
Size: 2085 183
SgrAI Cr'CCGG_yG
Cuts at: 0 1447 2268
Size: 1447 821
SmaI CCC'GGG
Cuts at: 0 29 158 180 227 255 317 534 1975
Size: 29 129 22 47 28 62 217 1441
Cuts at: 1975 2268
Size: 293
Fragments arranged by size:
1441 293 217 129 62 47 29 28
22
SmlI C'TyrA_G
Cuts at: 0 1903 2268
Size: 1903 365
StuI AGG'CCT
Cuts at: 0 1116 2268
Size: 1116 1152
StyI C'CwwG_G
Cuts at: 0 780 1070 1086 1125 1328 2268
Size: 780 290 16 39 203 940
Fragments arranged by size:
940 780 290 203 39 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1867 2268
Size: 1867 401
TatI w'GTAC_w
Cuts at: 0 731 2268
Size: 731 1537
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1248 1280 1573 1605 2268
Size: 1248 32 293 32 663
Fragments arranged by size:
1248 663 293 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 889 2268
Size: 889 1379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 826 1290 1335 2268
Size: 826 464 45 933
Fragments arranged by size:
933 826 464 45
XmaI C'CCGG_G
Cuts at: 0 27 156 178 225 253 315 532 1973
Size: 27 129 22 47 28 62 217 1441
Cuts at: 1973 2268
Size: 295
Fragments arranged by size:
1441 295 217 129 62 47 28 27
22
ZraI GAC'GTC
Cuts at: 0 477 2268
Size: 477 1791
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AloI ApoI AscI AseI
AsiSI AvrII BaeI BciVI BfrBI BglII BlpI BmgBI
BmtI BsaAI BsaBI BsiWI BsmI BspEI BspHI BsrBI
BsrGI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII EcoRI EcoRV FspI FspAI HindIII HpaI MfeI
MluI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SexAI SgfI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI