Restriction map for
pF1KSDA0666
(Linear) (Six-base) MAPSORT of: KIAA0666.seq Check: 7174 from: 1 to: 3234
KIAA0666.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1509 3234
Size: 1509 1725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1813 2793 3234
Size: 97 1716 980 441
Fragments arranged by size:
1716 980 441 97
AflIII A'CryG_T
Cuts at: 0 2177 3234
Size: 2177 1057
AleI CACnn'nnGTG
Cuts at: 0 2587 3234
Size: 2587 647
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 151 183 1972 2004 3234
Size: 151 32 1789 32 1230
Fragments arranged by size:
1789 1230 151 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 633 1395 2439 3234
Size: 633 762 1044 795
Fragments arranged by size:
1044 795 762 633
ApaI G_GGCC'C
Cuts at: 0 1616 3234
Size: 1616 1618
ApoI r'AATT_y
Cuts at: 0 310 919 935 972 1033 1913 2197 3234
Size: 310 609 16 37 61 880 284 1037
Fragments arranged by size:
1037 880 609 310 284 61 37 16
AseI AT'TA_AT
Cuts at: 0 614 778 854 2300 3234
Size: 614 164 76 1446 934
Fragments arranged by size:
1446 934 614 164 76
AvaI C'yCGr_G
Cuts at: 0 700 1855 3234
Size: 700 1155 1379
Fragments arranged by size:
1379 1155 700
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1889 1922 3234
Size: 1889 33 1312
Fragments arranged by size:
1889 1312 33
BanI G'GyrC_C
Cuts at: 0 682 2422 3234
Size: 682 1740 812
Fragments arranged by size:
1740 812 682
BanII G_rGCy'C
Cuts at: 0 588 1553 1616 2079 3012 3234
Size: 588 965 63 463 933 222
Fragments arranged by size:
965 933 588 463 222 63
BbeI G_GCGC'C
Cuts at: 0 686 3234
Size: 686 2548
BbsI GAAGACnn'nnnn_
Cuts at: 0 1899 1934 2834 3234
Size: 1899 35 900 400
Fragments arranged by size:
1899 900 400 35
BbvCI CC'TCA_GC
Cuts at: 0 1811 3234
Size: 1811 1423
BciVI GTATCCnnnnn_n'
Cuts at: 0 490 3234
Size: 490 2744
BclI T'GATC_A
Cuts at: 0 63 321 2928 3234
Size: 63 258 2607 306
Fragments arranged by size:
2607 306 258 63
BfrBI ATG'CAT
Cuts at: 0 2494 3234
Size: 2494 740
BglII A'GATC_T
Cuts at: 0 1920 2163 3234
Size: 1920 243 1071
Fragments arranged by size:
1920 1071 243
BlpI GC'TnA_GC
Cuts at: 0 865 1552 3023 3234
Size: 865 687 1471 211
Fragments arranged by size:
1471 865 687 211
Bme1580I G_kGCm'C
Cuts at: 0 1616 2427 3234
Size: 1616 811 807
Fragments arranged by size:
1616 811 807
BmgBI CAC'GTC
Cuts at: 0 591 3234
Size: 591 2643
BmrI ACTGGGnnnn_n'
Cuts at: 0 812 2600 3234
Size: 812 1788 634
Fragments arranged by size:
1788 812 634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1619 1694 2461 3234
Size: 1619 75 767 773
Fragments arranged by size:
1619 773 767 75
Bpu10I CC'TnA_GC
Cuts at: 0 1811 3123 3234
Size: 1811 1312 111
Fragments arranged by size:
1811 1312 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 814 1415 2413 2630 2715 3234
Size: 560 254 601 998 217 85 519
Fragments arranged by size:
998 601 560 519 254 217 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 505 3204 3234
Size: 505 2699 30
Fragments arranged by size:
2699 505 30
BsaAI yAC'GTr
Cuts at: 0 80 3234
Size: 80 3154
BsaBI GATnn'nnATC
Cuts at: 0 1237 3234
Size: 1237 1997
BsaHI Gr'CG_yC
Cuts at: 0 683 3234
Size: 683 2551
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1252 1282 1972 2002 3234
Size: 1252 30 690 30 1232
Fragments arranged by size:
1252 1232 690 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 1194 1712 1715 1730 3234
Size: 166 1028 518 3 15 1504
Fragments arranged by size:
1504 1028 518 166 15 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 896 1135 3234
Size: 718 178 239 2099
Fragments arranged by size:
2099 718 239 178
BsiHKAI G_wGCw'C
Cuts at: 0 640 866 1376 1553 1605 1776 2079 3012
Size: 640 226 510 177 52 171 303 933
Cuts at: 3012 3234
Size: 222
Fragments arranged by size:
933 640 510 303 226 222 177 171
52
BsiWI C'GTAC_G
Cuts at: 0 1305 3234
Size: 1305 1929
BsmI GAATG_Cn'
Cuts at: 0 1805 2496 3234
Size: 1805 691 738
Fragments arranged by size:
1805 738 691
Bsp1286I G_dGCh'C
Cuts at: 0 588 640 866 1376 1553 1605 1616 1776
Size: 588 52 226 510 177 52 11 160
Cuts at: 1776 2079 2427 3012 3234
Size: 303 348 585 222
Fragments arranged by size:
588 585 510 348 303 226 222 177
160 52 52 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1509 3234
Size: 1509 1725
BsrDI GCAATG_nn'
Cuts at: 0 130 3234
Size: 130 3104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 729 2319 3234
Size: 729 1590 915
Fragments arranged by size:
1590 915 729
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1278 3234
Size: 1278 1956
BstYI r'GATC_y
Cuts at: 0 1636 1920 2163 3234
Size: 1636 284 243 1071
Fragments arranged by size:
1636 1071 284 243
Bsu36I CC'TnA_GG
Cuts at: 0 1746 3234
Size: 1746 1488
BtgI C'CryG_G
Cuts at: 0 119 335 500 3234
Size: 119 216 165 2734
Fragments arranged by size:
2734 216 165 119
BtsI GCAGTG_nn'
Cuts at: 0 866 1155 1345 2873 3234
Size: 866 289 190 1528 361
Fragments arranged by size:
1528 866 361 289 190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1164 1199 3234
Size: 1164 35 2035
Fragments arranged by size:
2035 1164 35
DraI TTT'AAA
Cuts at: 0 410 3234
Size: 410 2824
DraIII CAC_nnn'GTG
Cuts at: 0 1120 3234
Size: 1120 2114
DrdI GACnn_nn'nnGTC
Cuts at: 0 519 3234
Size: 519 2715
EaeI y'GGCC_r
Cuts at: 0 662 2261 3234
Size: 662 1599 973
Fragments arranged by size:
1599 973 662
EarI CTCTTCn'nnn_
Cuts at: 0 1099 1436 2399 2879 3234
Size: 1099 337 963 480 355
Fragments arranged by size:
1099 963 480 355 337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1540 3234
Size: 1540 1694
EcoICRI GAG'CTC
Cuts at: 0 1551 2077 3010 3234
Size: 1551 526 933 224
Fragments arranged by size:
1551 933 526 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1619 1694 1813 2461 2793 3234
Size: 97 1522 75 119 648 332 441
Fragments arranged by size:
1522 648 441 332 119 97 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1670 1730 1745 2230 3234
Size: 1670 60 15 485 1004
Fragments arranged by size:
1670 1004 485 60 15
EcoO109I rG'GnC_Cy
Cuts at: 0 761 1260 1612 1613 1735 3234
Size: 761 499 352 1 122 1499
Fragments arranged by size:
1499 761 499 352 122 1
EcoRI G'AATT_C
Cuts at: 0 310 935 3234
Size: 310 625 2299
Fragments arranged by size:
2299 625 310
EcoRV GAT'ATC
Cuts at: 0 2510 3234
Size: 2510 724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1495 3234
Size: 1495 1739
HaeII r_GCGC'y
Cuts at: 0 686 3234
Size: 686 2548
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 1252 1284 2052 2084 2524 2556
Size: 457 32 763 32 768 32 440 32
Cuts at: 2556 3234
Size: 678
Fragments arranged by size:
768 763 678 457 440 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1124 3234
Size: 1124 2110
KasI G'GCGC_C
Cuts at: 0 682 3234
Size: 682 2552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2050 2162 3234
Size: 2050 112 1072
Fragments arranged by size:
2050 1072 112
MscI TGG'CCA
Cuts at: 0 2263 3234
Size: 2263 971
MslI CAynn'nnrTG
Cuts at: 0 2587 3234
Size: 2587 647
MspA1I CmG'CkG
Cuts at: 0 235 3234
Size: 235 2999
NarI GG'CG_CC
Cuts at: 0 683 3234
Size: 683 2551
NcoI C'CATG_G
Cuts at: 0 119 335 500 3234
Size: 119 216 165 2734
Fragments arranged by size:
2734 216 165 119
NdeI CA'TA_TG
Cuts at: 0 2496 3234
Size: 2496 738
NsiI A_TGCA'T
Cuts at: 0 2496 3234
Size: 2496 738
NspI r_CATG'y
Cuts at: 0 2181 3234
Size: 2181 1053
PacI TTA_AT'TAA
Cuts at: 0 782 3234
Size: 782 2452
PasI CC'CwG_GG
Cuts at: 0 1732 3234
Size: 1732 1502
PciI A'CATG_T
Cuts at: 0 2177 3234
Size: 2177 1057
PflMI CCAn_nnn'nTGG
Cuts at: 0 1768 3234
Size: 1768 1466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 1972 2004 3234
Size: 179 32 1761 32 1230
Fragments arranged by size:
1761 1230 179 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 761 1260 3234
Size: 761 499 1974
Fragments arranged by size:
1974 761 499
PspOMI G'GGCC_C
Cuts at: 0 1612 3234
Size: 1612 1622
PstI C_TGCA'G
Cuts at: 0 725 3234
Size: 725 2509
PvuII CAG'CTG
Cuts at: 0 235 3234
Size: 235 2999
SacI G_AGCT'C
Cuts at: 0 1553 2079 3012 3234
Size: 1553 526 933 222
Fragments arranged by size:
1553 933 526 222
ScaI AGT'ACT
Cuts at: 0 1201 3234
Size: 1201 2033
SexAI A'CCwGG_T
Cuts at: 0 1671 3234
Size: 1671 1563
SfcI C'TryA_G
Cuts at: 0 258 398 721 3234
Size: 258 140 323 2513
Fragments arranged by size:
2513 323 258 140
SfoI GGC'GCC
Cuts at: 0 684 3234
Size: 684 2550
SmaI CCC'GGG
Cuts at: 0 702 3234
Size: 702 2532
SmlI C'TyrA_G
Cuts at: 0 575 829 1430 2428 2645 2694 3234
Size: 575 254 601 998 217 49 540
Fragments arranged by size:
998 601 575 540 254 217 49
SspI AAT'ATT
Cuts at: 0 1293 3234
Size: 1293 1941
StyI C'CwwG_G
Cuts at: 0 119 335 500 870 2171 2264 3234
Size: 119 216 165 370 1301 93 970
Fragments arranged by size:
1301 970 370 216 165 119 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 83 1900 3234
Size: 83 1817 1334
Fragments arranged by size:
1817 1334 83
TatI w'GTAC_w
Cuts at: 0 1199 2330 3234
Size: 1199 1131 904
Fragments arranged by size:
1199 1131 904
XbaI T'CTAG_A
Cuts at: 0 1917 3234
Size: 1917 1317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 2178 3234
Size: 144 2034 1056
Fragments arranged by size:
2034 1056 144
XmaI C'CCGG_G
Cuts at: 0 700 3234
Size: 700 2534
XmnI GAAnn'nnTTC
Cuts at: 0 1055 1834 2387 2831 3234
Size: 1055 779 553 444 403
Fragments arranged by size:
1055 779 553 444 403
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApoI
AseI AvaI BaeI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrDI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII KasI
MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI
NspI PacI PasI PciI PflMI PpiI PpuMI PspOMI
PstI PvuII SacI ScaI SexAI SfcI SfoI SmaI
SmlI SspI StyI TaqII TatI XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaLI AscI AsiSI AvrII BamHI BcgI BglI
BmtI BplI BsaWI BseYI BsiEI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI EagI FseI FspI FspAI HincII HpaI
KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XhoI ZraI