Restriction map for
pF1KA0672
(Linear) (Six-base) MAPSORT of: KIAA0672.seq Check: 267 from: 1 to: 2454
KIAA0672.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 2311 2362 2454
Size: 2311 51 92
Fragments arranged by size:
2311 92 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 470 916 1063 2454
Size: 73 397 446 147 1391
Fragments arranged by size:
1391 446 397 147 73
AfeI AGC'GCT
Cuts at: 0 193 2454
Size: 193 2261
AflIII A'CryG_T
Cuts at: 0 1383 2454
Size: 1383 1071
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1295 1327 1333 1365 2454
Size: 1295 32 6 32 1089
Fragments arranged by size:
1295 1089 32 32 6
AlwNI CAG_nnn'CTG
Cuts at: 0 184 231 324 1692 1795 2410 2454
Size: 184 47 93 1368 103 615 44
Fragments arranged by size:
1368 615 184 103 93 47 44
ApaI G_GGCC'C
Cuts at: 0 1751 2454
Size: 1751 703
ApoI r'AATT_y
Cuts at: 0 1147 2454
Size: 1147 1307
AvaI C'yCGr_G
Cuts at: 0 1453 1750 2103 2356 2454
Size: 1453 297 353 253 98
Fragments arranged by size:
1453 353 297 253 98
BamHI G'GATC_C
Cuts at: 0 1040 2454
Size: 1040 1414
BanI G'GyrC_C
Cuts at: 0 517 1445 1879 1953 2454
Size: 517 928 434 74 501
Fragments arranged by size:
928 517 501 434 74
BanII G_rGCy'C
Cuts at: 0 368 1751 1860 1872 2357 2454
Size: 368 1383 109 12 485 97
Fragments arranged by size:
1383 485 368 109 97 12
BbeI G_GCGC'C
Cuts at: 0 1449 1883 2454
Size: 1449 434 571
Fragments arranged by size:
1449 571 434
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 476 2454
Size: 86 390 1978
Fragments arranged by size:
1978 390 86
BbvCI CC'TCA_GC
Cuts at: 0 1474 2222 2454
Size: 1474 748 232
Fragments arranged by size:
1474 748 232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 1639 1673 2454
Size: 32 1607 34 781
Fragments arranged by size:
1607 781 34 32
BglI GCCn_nnn'nGGC
Cuts at: 0 1654 2454
Size: 1654 800
BglII A'GATC_T
Cuts at: 0 2316 2454
Size: 2316 138
BlpI GC'TnA_GC
Cuts at: 0 1871 2454
Size: 1871 583
Bme1580I G_kGCm'C
Cuts at: 0 1751 1831 2454
Size: 1751 80 623
Fragments arranged by size:
1751 623 80
BmgBI CAC'GTC
Cuts at: 0 357 2454
Size: 357 2097
BmrI ACTGGGnnnn_n'
Cuts at: 0 1190 1578 2020 2454
Size: 1190 388 442 434
Fragments arranged by size:
1190 442 434 388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2099 2225 2454
Size: 2099 126 229
Fragments arranged by size:
2099 229 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 790 1522 1723 1772 1803 1886 2404
Size: 127 663 732 201 49 31 83 518
Cuts at: 2404 2454
Size: 50
Fragments arranged by size:
732 663 518 201 127 83 50 49
31
Bpu10I CC'TnA_GC
Cuts at: 0 317 1474 2189 2222 2454
Size: 317 1157 715 33 232
Fragments arranged by size:
1157 715 317 232 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 2454
Size: 859 1595
BsaI GGTCTCn'nnnn_
Cuts at: 0 2196 2454
Size: 2196 258
BsaAI yAC'GTr
Cuts at: 0 1386 2454
Size: 1386 1068
BsaHI Gr'CG_yC
Cuts at: 0 1446 1880 2454
Size: 1446 434 574
Fragments arranged by size:
1446 574 434
BsaWI w'CCGG_w
Cuts at: 0 1931 2454
Size: 1931 523
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 1211 1241 1333 1363 2419 2449
Size: 178 30 1003 30 92 30 1056 30
Cuts at: 2449 2454
Size: 5
Fragments arranged by size:
1056 1003 178 92 30 30 30 30
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 787 871 1578 2120 2210 2443 2446 2454
Size: 787 84 707 542 90 233 3 8
Fragments arranged by size:
787 707 542 233 90 84 8 3
BseYI C'CCAG_C
Cuts at: 0 1888 1894 2050 2118 2454
Size: 1888 6 156 68 336
Fragments arranged by size:
1888 336 156 68 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1781 1859 2454
Size: 958 823 78 595
Fragments arranged by size:
958 823 595 78
BsiEI CG_ry'CG
Cuts at: 0 795 1595 1599 2454
Size: 795 800 4 855
Fragments arranged by size:
855 800 795 4
BsiHKAI G_wGCw'C
Cuts at: 0 779 1872 2357 2390 2446 2454
Size: 779 1093 485 33 56 8
Fragments arranged by size:
1093 779 485 56 33 8
BsmI GAATG_Cn'
Cuts at: 0 1098 2454
Size: 1098 1356
BsmBI CGTCTCn'nnnn_
Cuts at: 0 292 347 2454
Size: 292 55 2107
Fragments arranged by size:
2107 292 55
Bsp1286I G_dGCh'C
Cuts at: 0 368 779 1751 1831 1860 1872 2357 2390
Size: 368 411 972 80 29 12 485 33
Cuts at: 2390 2446 2454
Size: 56 8
Fragments arranged by size:
972 485 411 368 80 56 33 29
12 8
BspEI T'CCGG_A
Cuts at: 0 1931 2454
Size: 1931 523
BsrFI r'CCGG_y
Cuts at: 0 1442 1466 1594 2454
Size: 1442 24 128 860
Fragments arranged by size:
1442 860 128 24
BsrGI T'GTAC_A
Cuts at: 0 602 2454
Size: 602 1852
BstXI CCAn_nnnn'nTGG
Cuts at: 0 567 1203 2011 2454
Size: 567 636 808 443
Fragments arranged by size:
808 636 567 443
BstYI r'GATC_y
Cuts at: 0 1040 2316 2454
Size: 1040 1276 138
Fragments arranged by size:
1276 1040 138
Bsu36I CC'TnA_GG
Cuts at: 0 535 1525 2084 2454
Size: 535 990 559 370
Fragments arranged by size:
990 559 535 370
BtgI C'CryG_G
Cuts at: 0 1484 1703 2264 2454
Size: 1484 219 561 190
Fragments arranged by size:
1484 561 219 190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1553 1588 2454
Size: 1553 35 866
Fragments arranged by size:
1553 866 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 480 1275 2454
Size: 480 795 1179
Fragments arranged by size:
1179 795 480
EaeI y'GGCC_r
Cuts at: 0 32 614 792 1234 1592 1980 2013 2454
Size: 32 582 178 442 358 388 33 441
Fragments arranged by size:
582 442 441 388 358 178 33 32
EagI C'GGCC_G
Cuts at: 0 792 1592 2454
Size: 792 800 862
Fragments arranged by size:
862 800 792
EarI CTCTTCn'nnn_
Cuts at: 0 545 871 1575 2454
Size: 545 326 704 879
Fragments arranged by size:
879 704 545 326
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 397 2359 2454
Size: 397 1962 95
Fragments arranged by size:
1962 397 95
EcoICRI GAG'CTC
Cuts at: 0 1870 2355 2454
Size: 1870 485 99
Fragments arranged by size:
1870 485 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 127 470 790 916 1063 1522 1723
Size: 73 54 343 320 126 147 459 201
Cuts at: 1723 1772 1803 1886 2404 2454
Size: 49 31 83 518 50
Fragments arranged by size:
518 459 343 320 201 147 126 83
73 54 50 49 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 2454
Size: 88 2366
EcoO109I rG'GnC_Cy
Cuts at: 0 1219 1971 2271 2454
Size: 1219 752 300 183
Fragments arranged by size:
1219 752 300 183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 103 771 1992 2454
Size: 103 668 1221 462
Fragments arranged by size:
1221 668 462 103
HaeII r_GCGC'y
Cuts at: 0 195 1449 1883 2454
Size: 195 1254 434 571
Fragments arranged by size:
1254 571 434 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 574 606 1211 1243 2275 2307
Size: 178 32 364 32 605 32 1032 32
Cuts at: 2307 2323 2344 2355 2376 2419 2451 2454
Size: 16 21 11 21 43 32 3
Fragments arranged by size:
1032 605 364 178 43 32 32 32
32 32 21 21 16 11 3
HincII GTy'rAC
Cuts at: 0 2363 2454
Size: 2363 91
HindIII A'AGCT_T
Cuts at: 0 1075 2454
Size: 1075 1379
KasI G'GCGC_C
Cuts at: 0 1445 1879 2454
Size: 1445 434 575
Fragments arranged by size:
1445 575 434
MfeI C'AATT_G
Cuts at: 0 965 1208 2454
Size: 965 243 1246
Fragments arranged by size:
1246 965 243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 2454
Size: 1579 875
MscI TGG'CCA
Cuts at: 0 34 616 1236 1982 2015 2454
Size: 34 582 620 746 33 439
Fragments arranged by size:
746 620 582 439 34 33
MslI CAynn'nnrTG
Cuts at: 0 1546 2454
Size: 1546 908
MspA1I CmG'CkG
Cuts at: 0 30 595 768 791 1677 1705 2011 2019
Size: 30 565 173 23 886 28 306 8
Cuts at: 2019 2122 2454
Size: 103 332
Fragments arranged by size:
886 565 332 306 173 103 30 28
23 8
NaeI GCC'GGC
Cuts at: 0 1468 2454
Size: 1468 986
NarI GG'CG_CC
Cuts at: 0 1446 1880 2454
Size: 1446 434 574
Fragments arranged by size:
1446 574 434
NgoMIV G'CCGG_C
Cuts at: 0 1466 2454
Size: 1466 988
NspI r_CATG'y
Cuts at: 0 24 158 1321 2292 2310 2394 2454
Size: 24 134 1163 971 18 84 60
Fragments arranged by size:
1163 971 134 84 60 24 18
PasI CC'CwG_GG
Cuts at: 0 1336 2296 2454
Size: 1336 960 158
Fragments arranged by size:
1336 960 158
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 1542 2454
Size: 45 1497 912
Fragments arranged by size:
1497 912 45
PfoI T'CCnGG_A
Cuts at: 0 1058 2454
Size: 1058 1396
PmlI CAC'GTG
Cuts at: 0 1386 2454
Size: 1386 1068
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1333 1365 2454
Size: 1333 32 1089
Fragments arranged by size:
1333 1089 32
PpuMI rG'GwC_Cy
Cuts at: 0 1219 1971 2454
Size: 1219 752 483
Fragments arranged by size:
1219 752 483
PshAI GACnn'nnGTC
Cuts at: 0 1916 2454
Size: 1916 538
PspOMI G'GGCC_C
Cuts at: 0 1747 2454
Size: 1747 707
PstI C_TGCA'G
Cuts at: 0 164 1911 2127 2454
Size: 164 1747 216 327
Fragments arranged by size:
1747 327 216 164
PvuII CAG'CTG
Cuts at: 0 30 595 2019 2122 2454
Size: 30 565 1424 103 332
Fragments arranged by size:
1424 565 332 103 30
SacI G_AGCT'C
Cuts at: 0 1872 2357 2454
Size: 1872 485 97
Fragments arranged by size:
1872 485 97
SacII CC_GC'GG
Cuts at: 0 1706 2454
Size: 1706 748
SalI G'TCGA_C
Cuts at: 0 2361 2454
Size: 2361 93
ScaI AGT'ACT
Cuts at: 0 946 2454
Size: 946 1508
SexAI A'CCwGG_T
Cuts at: 0 460 2454
Size: 460 1994
SfcI C'TryA_G
Cuts at: 0 160 1404 1907 2123 2312 2454
Size: 160 1244 503 216 189 142
Fragments arranged by size:
1244 503 216 189 160 142
SfoI GGC'GCC
Cuts at: 0 1447 1881 2454
Size: 1447 434 573
Fragments arranged by size:
1447 573 434
SmlI C'TyrA_G
Cuts at: 0 838 2454
Size: 838 1616
SphI G_CATG'C
Cuts at: 0 24 1321 2454
Size: 24 1297 1133
Fragments arranged by size:
1297 1133 24
SspI AAT'ATT
Cuts at: 0 402 2454
Size: 402 2052
StuI AGG'CCT
Cuts at: 0 736 2454
Size: 736 1718
StyI C'CwwG_G
Cuts at: 0 521 1112 1967 2096 2454
Size: 521 591 855 129 358
Fragments arranged by size:
855 591 521 358 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1534 1560 1980 2454
Size: 1534 26 420 474
Fragments arranged by size:
1534 474 420 26
TatI w'GTAC_w
Cuts at: 0 602 944 1380 2454
Size: 602 342 436 1074
Fragments arranged by size:
1074 602 436 342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 667 699 1083 1115 1955 1987 2454
Size: 667 32 384 32 840 32 467
Fragments arranged by size:
840 667 467 384 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 435 2454
Size: 42 393 2019
Fragments arranged by size:
2019 393 42
XmnI GAAnn'nnTTC
Cuts at: 0 10 83 2454
Size: 10 73 2371
Fragments arranged by size:
2371 73 10
Enzymes that do cut:
AccI AcuI AfeI AflIII AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BsrFI BsrGI BstXI BstYI Bsu36I BtgI CspCI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HindIII KasI MfeI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SalI ScaI SexAI SfcI SfoI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BmtI BsaBI BsiWI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
BtsI ClaI DraI DraIII EcoRI EcoRV FseI FspI
FspAI HpaI KpnI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PsiI PspXI PsrI
PvuI RsrII SanDI SapI SbfI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI
XmaI ZraI