Restriction map for
pF1KSDA0677
(Linear) (Six-base) MAPSORT of: KIAA0677.seq Check: 2991 from: 1 to: 3192
KIAA0677.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 862 3008 3192
Size: 862 2146 184
Fragments arranged by size:
2146 862 184
Acc65I G'GTAC_C
Cuts at: 0 412 3192
Size: 412 2780
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 587 769 1174 1400 2060 2294 2793
Size: 63 524 182 405 226 660 234 499
Cuts at: 2793 2894 3192
Size: 101 298
Fragments arranged by size:
660 524 499 405 298 234 226 182
101 63
AflII C'TTAA_G
Cuts at: 0 1060 3192
Size: 1060 2132
AflIII A'CryG_T
Cuts at: 0 1748 3192
Size: 1748 1444
AhdI GACnn_n'nnGTC
Cuts at: 0 1495 3192
Size: 1495 1697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1655 3192
Size: 1655 1537
ApaI G_GGCC'C
Cuts at: 0 2352 3192
Size: 2352 840
ApaLI G'TGCA_C
Cuts at: 0 1630 3192
Size: 1630 1562
ApoI r'AATT_y
Cuts at: 0 761 1387 1966 2041 3192
Size: 761 626 579 75 1151
Fragments arranged by size:
1151 761 626 579 75
AvaI C'yCGr_G
Cuts at: 0 349 3192
Size: 349 2843
BanI G'GyrC_C
Cuts at: 0 412 1193 2191 3192
Size: 412 781 998 1001
Fragments arranged by size:
1001 998 781 412
BanII G_rGCy'C
Cuts at: 0 117 350 1241 1540 1605 2352 3192
Size: 117 233 891 299 65 747 840
Fragments arranged by size:
891 840 747 299 233 117 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 550 575 1162 1491 2282 2622 3192
Size: 550 25 587 329 791 340 570
Fragments arranged by size:
791 587 570 550 340 329 25
BbvCI CC'TCA_GC
Cuts at: 0 1889 3192
Size: 1889 1303
BclI T'GATC_A
Cuts at: 0 803 3192
Size: 803 2389
BglI GCCn_nnn'nGGC
Cuts at: 0 1990 2680 3192
Size: 1990 690 512
Fragments arranged by size:
1990 690 512
BglII A'GATC_T
Cuts at: 0 941 3192
Size: 941 2251
Bme1580I G_kGCm'C
Cuts at: 0 1108 1291 1634 2352 3192
Size: 1108 183 343 718 840
Fragments arranged by size:
1108 840 718 343 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 23 3192
Size: 23 3169
BmtI G_CTAG'C
Cuts at: 0 133 3192
Size: 133 3059
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1608 2994 3192
Size: 1608 1386 198
Fragments arranged by size:
1608 1386 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 2839 3192
Size: 812 2027 353
Fragments arranged by size:
2027 812 353
Bpu10I CC'TnA_GC
Cuts at: 0 638 1889 3192
Size: 638 1251 1303
Fragments arranged by size:
1303 1251 638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 2200 3123 3192
Size: 767 1433 923 69
Fragments arranged by size:
1433 923 767 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 1142 1969 2767 3192
Size: 1142 827 798 425
Fragments arranged by size:
1142 827 798 425
BsaHI Gr'CG_yC
Cuts at: 0 1491 1785 3192
Size: 1491 294 1407
Fragments arranged by size:
1491 1407 294
BsaWI w'CCGG_w
Cuts at: 0 2442 3192
Size: 2442 750
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1111 1114 1126 1435 1444 1906 2197 2329
Size: 1111 3 12 309 9 462 291 132
Cuts at: 2329 2357 3192
Size: 28 835
Fragments arranged by size:
1111 835 462 309 291 132 28 12
9 3
BseYI C'CCAG_C
Cuts at: 0 1000 3192
Size: 1000 2192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 583 2241 2554 3152 3192
Size: 419 164 1658 313 598 40
Fragments arranged by size:
1658 598 419 313 164 40
BsiEI CG_ry'CG
Cuts at: 0 2550 3192
Size: 2550 642
BsiHKAI G_wGCw'C
Cuts at: 0 117 350 693 919 1241 1634 3192
Size: 117 233 343 226 322 393 1558
Fragments arranged by size:
1558 393 343 322 233 226 117
Bsp1286I G_dGCh'C
Cuts at: 0 117 350 693 919 1108 1241 1291 1540
Size: 117 233 343 226 189 133 50 249
Cuts at: 1540 1605 1634 2352 3192
Size: 65 29 718 840
Fragments arranged by size:
840 718 343 249 233 226 189 133
117 65 50 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 605 2647 3192
Size: 605 2042 545
Fragments arranged by size:
2042 605 545
BsrBI CCG'CTC
Cuts at: 0 354 1719 3192
Size: 354 1365 1473
Fragments arranged by size:
1473 1365 354
BsrDI GCAATG_nn'
Cuts at: 0 89 2426 3192
Size: 89 2337 766
Fragments arranged by size:
2337 766 89
BsrFI r'CCGG_y
Cuts at: 0 452 829 2590 3192
Size: 452 377 1761 602
Fragments arranged by size:
1761 602 452 377
BssSI C'ACGA_G
Cuts at: 0 164 1680 3192
Size: 164 1516 1512
Fragments arranged by size:
1516 1512 164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 909 1735 2219 3192
Size: 909 826 484 973
Fragments arranged by size:
973 909 826 484
BstEII G'GTnAC_C
Cuts at: 0 820 3192
Size: 820 2372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 155 620 2917 2931 3192
Size: 155 465 2297 14 261
Fragments arranged by size:
2297 465 261 155 14
BstYI r'GATC_y
Cuts at: 0 941 1254 1458 3192
Size: 941 313 204 1734
Fragments arranged by size:
1734 941 313 204
BstZ17I GTA'TAC
Cuts at: 0 3009 3192
Size: 3009 183
Bsu36I CC'TnA_GG
Cuts at: 0 1877 2828 3192
Size: 1877 951 364
Fragments arranged by size:
1877 951 364
BtgI C'CryG_G
Cuts at: 0 840 947 1979 3192
Size: 840 107 1032 1213
Fragments arranged by size:
1213 1032 840 107
BtsI GCAGTG_nn'
Cuts at: 0 1998 2542 2696 3192
Size: 1998 544 154 496
Fragments arranged by size:
1998 544 496 154
DrdI GACnn_nn'nnGTC
Cuts at: 0 231 2377 2911 3192
Size: 231 2146 534 281
Fragments arranged by size:
2146 534 281 231
EaeI y'GGCC_r
Cuts at: 0 450 1166 2547 2907 3192
Size: 450 716 1381 360 285
Fragments arranged by size:
1381 716 450 360 285
EagI C'GGCC_G
Cuts at: 0 2547 3192
Size: 2547 645
EarI CTCTTCn'nnn_
Cuts at: 0 59 338 1246 1388 1412 1551 1569 1757
Size: 59 279 908 142 24 139 18 188
Cuts at: 1757 1877 3017 3192
Size: 120 1140 175
Fragments arranged by size:
1140 908 279 188 175 142 139 120
59 24 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 703 3192
Size: 703 2489
EcoICRI GAG'CTC
Cuts at: 0 115 348 1239 3192
Size: 115 233 891 1953
Fragments arranged by size:
1953 891 233 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 587 769 812 1174 1400 2060 2294
Size: 63 524 182 43 362 226 660 234
Cuts at: 2294 2793 2839 2894 3192
Size: 499 46 55 298
Fragments arranged by size:
660 524 499 362 298 234 226 182
63 55 46 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2314 2873 3192
Size: 2314 559 319
Fragments arranged by size:
2314 559 319
EcoO109I rG'GnC_Cy
Cuts at: 0 1992 2253 2348 2349 2681 3192
Size: 1992 261 95 1 332 511
Fragments arranged by size:
1992 511 332 261 95 1
EcoRI G'AATT_C
Cuts at: 0 761 1966 3192
Size: 761 1205 1226
Fragments arranged by size:
1226 1205 761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 567 2700 3192
Size: 567 2133 492
Fragments arranged by size:
2133 567 492
HaeII r_GCGC'y
Cuts at: 0 1700 2739 3192
Size: 1700 1039 453
Fragments arranged by size:
1700 1039 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 1481 1513 1546 1578 1843 1875
Size: 36 32 1413 32 33 32 265 32
Cuts at: 1875 2016 2048 2274 2306 2788 2820 3192
Size: 141 32 226 32 482 32 372
Fragments arranged by size:
1413 482 372 265 226 141 36 33
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2424 2790 3192
Size: 2424 366 402
Fragments arranged by size:
2424 402 366
KpnI G_GTAC'C
Cuts at: 0 416 3192
Size: 416 2776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 635 3192
Size: 79 556 2557
Fragments arranged by size:
2557 556 79
MscI TGG'CCA
Cuts at: 0 1168 3192
Size: 1168 2024
MslI CAynn'nnrTG
Cuts at: 0 434 952 1637 3192
Size: 434 518 685 1555
Fragments arranged by size:
1555 685 518 434
MspA1I CmG'CkG
Cuts at: 0 219 1051 1450 1869 2552 3192
Size: 219 832 399 419 683 640
Fragments arranged by size:
832 683 640 419 399 219
NaeI GCC'GGC
Cuts at: 0 454 831 3192
Size: 454 377 2361
Fragments arranged by size:
2361 454 377
NcoI C'CATG_G
Cuts at: 0 840 947 1979 3192
Size: 840 107 1032 1213
Fragments arranged by size:
1213 1032 840 107
NgoMIV G'CCGG_C
Cuts at: 0 452 829 3192
Size: 452 377 2363
Fragments arranged by size:
2363 452 377
NheI G'CTAG_C
Cuts at: 0 129 3192
Size: 129 3063
NspI r_CATG'y
Cuts at: 0 433 540 1752 3072 3192
Size: 433 107 1212 1320 120
Fragments arranged by size:
1320 1212 433 120 107
PciI A'CATG_T
Cuts at: 0 1748 3192
Size: 1748 1444
PflMI CCAn_nnn'nTGG
Cuts at: 0 394 1087 3192
Size: 394 693 2105
Fragments arranged by size:
2105 693 394
PfoI T'CCnGG_A
Cuts at: 0 467 3192
Size: 467 2725
PpuMI rG'GwC_Cy
Cuts at: 0 2253 3192
Size: 2253 939
PspOMI G'GGCC_C
Cuts at: 0 2348 3192
Size: 2348 844
PspXI vC'TCGA_Gb
Cuts at: 0 349 3192
Size: 349 2843
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 505 537 3192
Size: 505 32 2655
Fragments arranged by size:
2655 505 32
PstI C_TGCA'G
Cuts at: 0 1835 2149 2357 2703 3192
Size: 1835 314 208 346 489
Fragments arranged by size:
1835 489 346 314 208
PvuII CAG'CTG
Cuts at: 0 219 1051 1450 1869 3192
Size: 219 832 399 419 1323
Fragments arranged by size:
1323 832 419 399 219
SacI G_AGCT'C
Cuts at: 0 117 350 1241 3192
Size: 117 233 891 1951
Fragments arranged by size:
1951 891 233 117
SanDI GG'GwC_CC
Cuts at: 0 2253 3192
Size: 2253 939
SapI GCTCTTCn'nnn_
Cuts at: 0 338 1246 1388 1551 3017 3192
Size: 338 908 142 163 1466 175
Fragments arranged by size:
1466 908 338 175 163 142
ScaI AGT'ACT
Cuts at: 0 316 3192
Size: 316 2876
SexAI A'CCwGG_T
Cuts at: 0 2957 3192
Size: 2957 235
SfcI C'TryA_G
Cuts at: 0 330 582 921 1481 1831 2145 2353 2699
Size: 330 252 339 560 350 314 208 346
Cuts at: 2699 3192
Size: 493
Fragments arranged by size:
560 493 350 346 339 330 314 252
208
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1990 3192
Size: 1990 1202
SmlI C'TyrA_G
Cuts at: 0 349 782 1060 2179 3138 3192
Size: 349 433 278 1119 959 54
Fragments arranged by size:
1119 959 433 349 278 54
SpeI A'CTAG_T
Cuts at: 0 1579 3192
Size: 1579 1613
SspI AAT'ATT
Cuts at: 0 447 3192
Size: 447 2745
StuI AGG'CCT
Cuts at: 0 1903 3192
Size: 1903 1289
StyI C'CwwG_G
Cuts at: 0 108 134 840 947 1247 1454 1605 1778
Size: 108 26 706 107 300 207 151 173
Cuts at: 1778 1979 2675 3192
Size: 201 696 517
Fragments arranged by size:
706 696 517 300 207 201 173 151
108 107 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1333 1835 3192
Size: 1333 502 1357
Fragments arranged by size:
1357 1333 502
TatI w'GTAC_w
Cuts at: 0 251 314 527 3192
Size: 251 63 213 2665
Fragments arranged by size:
2665 251 213 63
Tth111I GACn'n_nGTC
Cuts at: 0 1787 3192
Size: 1787 1405
XbaI T'CTAG_A
Cuts at: 0 3046 3192
Size: 3046 146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 3192
Size: 216 2976
XhoI C'TCGA_G
Cuts at: 0 349 3192
Size: 349 2843
XmnI GAAnn'nnTTC
Cuts at: 0 1966 2073 3026 3192
Size: 1966 107 953 166
Fragments arranged by size:
1966 953 166 107
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AhdI AlfI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BclI
BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KpnI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PpuMI PspOMI PspXI PsrI PstI PvuII
SacI SanDI SapI ScaI SexAI SfcI SfiI SmlI
SpeI SspI StuI StyI TaqII TatI Tth111I XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AleI AloI AlwNI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BfrBI BlpI BmgBI BsaAI BsaBI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI BtgZI
ClaI CspCI DraI DraIII EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI NarI NdeI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PsiI PvuI RsrII SacII SalI SbfI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI TstI XmaI
ZraI