Restriction map for
pF1KSDA0679
(Linear) (Six-base) MAPSORT of: KIAA0679.seq Check: 8747 from: 1 to: 2748
KIAA0679.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 2413 2748
Size: 2413 335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1985 2623 2748
Size: 1985 638 125
Fragments arranged by size:
1985 638 125
AflIII A'CryG_T
Cuts at: 0 1916 2748
Size: 1916 832
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1697 2748
Size: 1697 1051
ApaI G_GGCC'C
Cuts at: 0 883 2748
Size: 883 1865
ApoI r'AATT_y
Cuts at: 0 773 949 1741 2381 2657 2665 2748
Size: 773 176 792 640 276 8 83
Fragments arranged by size:
792 773 640 276 176 83 8
AvaI C'yCGr_G
Cuts at: 0 2618 2748
Size: 2618 130
AvrII C'CTAG_G
Cuts at: 0 615 2748
Size: 615 2133
BamHI G'GATC_C
Cuts at: 0 2390 2748
Size: 2390 358
BanI G'GyrC_C
Cuts at: 0 105 2748
Size: 105 2643
BanII G_rGCy'C
Cuts at: 0 50 171 822 883 1307 2748
Size: 50 121 651 61 424 1441
Fragments arranged by size:
1441 651 424 121 61 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 122 216 2588 2748
Size: 122 94 2372 160
Fragments arranged by size:
2372 160 122 94
BbvCI CC'TCA_GC
Cuts at: 0 2284 2748
Size: 2284 464
BciVI GTATCCnnnnn_n'
Cuts at: 0 462 2748
Size: 462 2286
BclI T'GATC_A
Cuts at: 0 858 2748
Size: 858 1890
BlpI GC'TnA_GC
Cuts at: 0 49 347 2748
Size: 49 298 2401
Fragments arranged by size:
2401 298 49
Bme1580I G_kGCm'C
Cuts at: 0 883 2347 2748
Size: 883 1464 401
Fragments arranged by size:
1464 883 401
BmtI G_CTAG'C
Cuts at: 0 1147 2748
Size: 1147 1601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 69 2748
Size: 69 2679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 238 806 1684 2748
Size: 106 132 568 878 1064
Fragments arranged by size:
1064 878 568 132 106
Bpu10I CC'TnA_GC
Cuts at: 0 2284 2748
Size: 2284 464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1350 2748
Size: 1350 1398
BsaI GGTCTCn'nnnn_
Cuts at: 0 2015 2634 2748
Size: 2015 619 114
Fragments arranged by size:
2015 619 114
BsaBI GATnn'nnATC
Cuts at: 0 857 2748
Size: 857 1891
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 131 161 1906 1936 2748
Size: 131 30 1745 30 812
Fragments arranged by size:
1745 812 131 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 2748
Size: 46 2702
BseYI C'CCAG_C
Cuts at: 0 133 2748
Size: 133 2615
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 359 516 563 2748
Size: 25 334 157 47 2185
Fragments arranged by size:
2185 334 157 47 25
BsiHKAI G_wGCw'C
Cuts at: 0 50 822 1728 2748
Size: 50 772 906 1020
Fragments arranged by size:
1020 906 772 50
Bsp1286I G_dGCh'C
Cuts at: 0 50 171 822 883 1307 1728 2347 2748
Size: 50 121 651 61 424 421 619 401
Fragments arranged by size:
651 619 424 421 401 121 61 50
BsrBI CCG'CTC
Cuts at: 0 36 142 2748
Size: 36 106 2606
Fragments arranged by size:
2606 106 36
BsrDI GCAATG_nn'
Cuts at: 0 1568 2748
Size: 1568 1180
BsrFI r'CCGG_y
Cuts at: 0 93 2748
Size: 93 2655
BsrGI T'GTAC_A
Cuts at: 0 1640 1971 2183 2748
Size: 1640 331 212 565
Fragments arranged by size:
1640 565 331 212
BssSI C'ACGA_G
Cuts at: 0 377 1826 2748
Size: 377 1449 922
Fragments arranged by size:
1449 922 377
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1614 2748
Size: 1614 1134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 830 1398 2748
Size: 830 568 1350
Fragments arranged by size:
1350 830 568
BstYI r'GATC_y
Cuts at: 0 895 2390 2748
Size: 895 1495 358
Fragments arranged by size:
1495 895 358
BstZ17I GTA'TAC
Cuts at: 0 2414 2748
Size: 2414 334
Bsu36I CC'TnA_GG
Cuts at: 0 2165 2748
Size: 2165 583
BtgI C'CryG_G
Cuts at: 0 913 1391 2748
Size: 913 478 1357
Fragments arranged by size:
1357 913 478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 65 2748
Size: 65 2683
BtsI GCAGTG_nn'
Cuts at: 0 1095 1643 2748
Size: 1095 548 1105
Fragments arranged by size:
1105 1095 548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2705 2740 2748
Size: 2705 35 8
Fragments arranged by size:
2705 35 8
DraI TTT'AAA
Cuts at: 0 1268 2748
Size: 1268 1480
DraIII CAC_nnn'GTG
Cuts at: 0 1213 2748
Size: 1213 1535
EaeI y'GGCC_r
Cuts at: 0 149 2567 2748
Size: 149 2418 181
Fragments arranged by size:
2418 181 149
EarI CTCTTCn'nnn_
Cuts at: 0 643 788 859 1838 2748
Size: 643 145 71 979 910
Fragments arranged by size:
979 910 643 145 71
EcoICRI GAG'CTC
Cuts at: 0 48 820 2748
Size: 48 772 1928
Fragments arranged by size:
1928 772 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 238 806 1684 1985 2623 2748
Size: 106 132 568 878 301 638 125
Fragments arranged by size:
878 638 568 301 132 125 106
EcoO109I rG'GnC_Cy
Cuts at: 0 751 2748
Size: 751 1997
EcoRI G'AATT_C
Cuts at: 0 2657 2748
Size: 2657 91
EcoRV GAT'ATC
Cuts at: 0 426 1938 2748
Size: 426 1512 810
Fragments arranged by size:
1512 810 426
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 1906 1938 2161 2193 2748
Size: 410 32 1464 32 223 32 555
Fragments arranged by size:
1464 555 410 223 32 32 32
HindIII A'AGCT_T
Cuts at: 0 718 2748
Size: 718 2030
MfeI C'AATT_G
Cuts at: 0 1782 1887 2081 2748
Size: 1782 105 194 667
Fragments arranged by size:
1782 667 194 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 80 2719 2748
Size: 8 72 2639 29
Fragments arranged by size:
2639 72 29 8
MscI TGG'CCA
Cuts at: 0 2569 2748
Size: 2569 179
MslI CAynn'nnrTG
Cuts at: 0 848 1396 1402 1433 2748
Size: 848 548 6 31 1315
Fragments arranged by size:
1315 848 548 31 6
NaeI GCC'GGC
Cuts at: 0 95 2748
Size: 95 2653
NcoI C'CATG_G
Cuts at: 0 1391 2748
Size: 1391 1357
NdeI CA'TA_TG
Cuts at: 0 2326 2748
Size: 2326 422
NgoMIV G'CCGG_C
Cuts at: 0 93 2748
Size: 93 2655
NheI G'CTAG_C
Cuts at: 0 1143 2748
Size: 1143 1605
NspI r_CATG'y
Cuts at: 0 1920 2748
Size: 1920 828
PasI CC'CwG_GG
Cuts at: 0 2033 2748
Size: 2033 715
PciI A'CATG_T
Cuts at: 0 1916 2748
Size: 1916 832
PflMI CCAn_nnn'nTGG
Cuts at: 0 2086 2748
Size: 2086 662
PfoI T'CCnGG_A
Cuts at: 0 2386 2748
Size: 2386 362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 2748
Size: 379 32 2337
Fragments arranged by size:
2337 379 32
PpuMI rG'GwC_Cy
Cuts at: 0 751 2748
Size: 751 1997
PspOMI G'GGCC_C
Cuts at: 0 879 2748
Size: 879 1869
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2471 2503 2748
Size: 2471 32 245
Fragments arranged by size:
2471 245 32
PstI C_TGCA'G
Cuts at: 0 162 2748
Size: 162 2586
SacI G_AGCT'C
Cuts at: 0 50 822 2748
Size: 50 772 1926
Fragments arranged by size:
1926 772 50
ScaI AGT'ACT
Cuts at: 0 1053 2489 2748
Size: 1053 1436 259
Fragments arranged by size:
1436 1053 259
SexAI A'CCwGG_T
Cuts at: 0 476 988 2748
Size: 476 512 1760
Fragments arranged by size:
1760 512 476
SfcI C'TryA_G
Cuts at: 0 158 1119 2348 2748
Size: 158 961 1229 400
Fragments arranged by size:
1229 961 400 158
SmaI CCC'GGG
Cuts at: 0 2620 2748
Size: 2620 128
SmlI C'TyrA_G
Cuts at: 0 1329 2748
Size: 1329 1419
SpeI A'CTAG_T
Cuts at: 0 1857 2748
Size: 1857 891
StyI C'CwwG_G
Cuts at: 0 214 443 615 1355 1391 2339 2748
Size: 214 229 172 740 36 948 409
Fragments arranged by size:
948 740 409 229 214 172 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 482 700 2748
Size: 482 218 2048
Fragments arranged by size:
2048 482 218
TatI w'GTAC_w
Cuts at: 0 1051 1113 1640 1919 1971 2183 2487 2748
Size: 1051 62 527 279 52 212 304 261
Fragments arranged by size:
1051 527 304 279 261 212 62 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 131 163 696 722 728 754 2748
Size: 131 32 533 26 6 26 1994
Fragments arranged by size:
1994 533 131 32 26 26 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1398 2577 2748
Size: 1398 1179 171
Fragments arranged by size:
1398 1179 171
XmaI C'CCGG_G
Cuts at: 0 2618 2748
Size: 2618 130
XmnI GAAnn'nnTTC
Cuts at: 0 719 2711 2748
Size: 719 1992 37
Fragments arranged by size:
1992 719 37
Enzymes that do cut:
AccI AcuI AflIII AlfI ApaI ApoI AvaI AvrII
BamHI BanI BanII BbsI BbvCI BciVI BclI BlpI
Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HindIII MfeI
MmeI MscI MslI NaeI NcoI NdeI NgoMIV NheI
NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI
PsrI PstI SacI ScaI SexAI SfcI SmaI SmlI
SpeI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaLI AscI AseI AsiSI BaeI
BbeI BcgI BfrBI BglI BglII BmgBI BmrI BsaAI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BssHII BstBI BstEII ClaI DrdI EagI EciI
EcoNI FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI MspA1I NarI NotI NruI NsiI
PacI PmeI PmlI PshAI PsiI PspXI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI ZraI