Restriction map for
pF1KSDA0680
(Linear) (Six-base) MAPSORT of: KIAA0680.seq Check: 1230 from: 1 to: 1902
KIAA0680.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1809 1902
Size: 1809 93
AccI GT'mk_AC
Cuts at: 0 1875 1902
Size: 1875 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1148 1253 1526 1601 1902
Size: 374 774 105 273 75 301
Fragments arranged by size:
774 374 301 273 105 75
AflII C'TTAA_G
Cuts at: 0 241 1170 1902
Size: 241 929 732
Fragments arranged by size:
929 732 241
AleI CACnn'nnGTG
Cuts at: 0 557 1902
Size: 557 1345
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 908 940 1902
Size: 908 32 962
Fragments arranged by size:
962 908 32
AlwNI CAG_nnn'CTG
Cuts at: 0 457 1165 1242 1902
Size: 457 708 77 660
Fragments arranged by size:
708 660 457 77
ApoI r'AATT_y
Cuts at: 0 160 288 955 1837 1902
Size: 160 128 667 882 65
Fragments arranged by size:
882 667 160 128 65
AvaI C'yCGr_G
Cuts at: 0 329 650 1902
Size: 329 321 1252
Fragments arranged by size:
1252 329 321
BamHI G'GATC_C
Cuts at: 0 1715 1902
Size: 1715 187
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 1231 1376 1902
Size: 154 1077 145 526
Fragments arranged by size:
1077 526 154 145
BbvCI CC'TCA_GC
Cuts at: 0 935 1235 1902
Size: 935 300 667
Fragments arranged by size:
935 667 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 689 723 1902
Size: 689 34 1179
Fragments arranged by size:
1179 689 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1422 1902
Size: 1422 480
BclI T'GATC_A
Cuts at: 0 255 1902
Size: 255 1647
BlpI GC'TnA_GC
Cuts at: 0 1561 1675 1902
Size: 1561 114 227
Fragments arranged by size:
1561 227 114
Bme1580I G_kGCm'C
Cuts at: 0 302 1902
Size: 302 1600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1448 1902
Size: 1448 454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 950 974 1040 1902
Size: 415 535 24 66 862
Fragments arranged by size:
862 535 415 66 24
Bpu10I CC'TnA_GC
Cuts at: 0 482 935 1235 1902
Size: 482 453 300 667
Fragments arranged by size:
667 482 453 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1902
Size: 1045 857
BsaI GGTCTCn'nnnn_
Cuts at: 0 883 1677 1902
Size: 883 794 225
Fragments arranged by size:
883 794 225
BsaBI GATnn'nnATC
Cuts at: 0 310 1902
Size: 310 1592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 579 609 1737 1767 1902
Size: 579 30 1128 30 135
Fragments arranged by size:
1128 579 135 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 467 657 952 992 1228 1902
Size: 232 235 190 295 40 236 674
Fragments arranged by size:
674 295 236 235 232 190 40
BseYI C'CCAG_C
Cuts at: 0 541 1218 1902
Size: 541 677 684
Fragments arranged by size:
684 677 541
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1098 1902
Size: 1098 804
Bsp1286I G_dGCh'C
Cuts at: 0 302 1902
Size: 302 1600
BspHI T'CATG_A
Cuts at: 0 1863 1902
Size: 1863 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 1094 1809 1902
Size: 52 1042 715 93
Fragments arranged by size:
1042 715 93 52
BsrFI r'CCGG_y
Cuts at: 0 447 1902
Size: 447 1455
BsrGI T'GTAC_A
Cuts at: 0 1349 1902
Size: 1349 553
BstAPI GCAn_nnn'nTGC
Cuts at: 0 457 1902
Size: 457 1445
BstYI r'GATC_y
Cuts at: 0 490 1715 1902
Size: 490 1225 187
Fragments arranged by size:
1225 490 187
BtgI C'CryG_G
Cuts at: 0 965 1902
Size: 965 937
BtsI GCAGTG_nn'
Cuts at: 0 536 1395 1902
Size: 536 859 507
Fragments arranged by size:
859 536 507
DraI TTT'AAA
Cuts at: 0 1842 1902
Size: 1842 60
DrdI GACnn_nn'nnGTC
Cuts at: 0 1090 1902
Size: 1090 812
EarI CTCTTCn'nnn_
Cuts at: 0 1124 1505 1619 1862 1902
Size: 1124 381 114 243 40
Fragments arranged by size:
1124 381 243 114 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 415 950 974 1040 1148 1253 1526
Size: 374 41 535 24 66 108 105 273
Cuts at: 1526 1601 1902
Size: 75 301
Fragments arranged by size:
535 374 301 273 108 105 75 66
41 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 527 1788 1902
Size: 527 1261 114
Fragments arranged by size:
1261 527 114
EcoO109I rG'GnC_Cy
Cuts at: 0 609 1902
Size: 609 1293
EcoRV GAT'ATC
Cuts at: 0 197 1902
Size: 197 1705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1218 1250 1315 1347 1902
Size: 1218 32 65 32 555
Fragments arranged by size:
1218 555 65 32 32
HincII GTy'rAC
Cuts at: 0 15 279 1179 1796 1876 1902
Size: 15 264 900 617 80 26
Fragments arranged by size:
900 617 264 80 26 15
HindIII A'AGCT_T
Cuts at: 0 29 743 1902
Size: 29 714 1159
Fragments arranged by size:
1159 714 29
HpaI GTT'AAC
Cuts at: 0 279 1179 1796 1902
Size: 279 900 617 106
Fragments arranged by size:
900 617 279 106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1056 1551 1902
Size: 890 166 495 351
Fragments arranged by size:
890 495 351 166
MslI CAynn'nnrTG
Cuts at: 0 557 1852 1902
Size: 557 1295 50
Fragments arranged by size:
1295 557 50
NcoI C'CATG_G
Cuts at: 0 965 1902
Size: 965 937
PasI CC'CwG_GG
Cuts at: 0 1784 1902
Size: 1784 118
PshAI GACnn'nnGTC
Cuts at: 0 1109 1902
Size: 1109 793
PstI C_TGCA'G
Cuts at: 0 888 1806 1902
Size: 888 918 96
Fragments arranged by size:
918 888 96
SalI G'TCGA_C
Cuts at: 0 1874 1902
Size: 1874 28
SapI GCTCTTCn'nnn_
Cuts at: 0 1124 1902
Size: 1124 778
SfcI C'TryA_G
Cuts at: 0 35 884 1802 1902
Size: 35 849 918 100
Fragments arranged by size:
918 849 100 35
SmlI C'TyrA_G
Cuts at: 0 241 1024 1170 1902
Size: 241 783 146 732
Fragments arranged by size:
783 732 241 146
StyI C'CwwG_G
Cuts at: 0 127 590 791 965 1902
Size: 127 463 201 174 937
Fragments arranged by size:
937 463 201 174 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 639 1902
Size: 639 1263
TatI w'GTAC_w
Cuts at: 0 1349 1902
Size: 1349 553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1737 1769 1902
Size: 1737 32 133
Fragments arranged by size:
1737 133 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1215 1902
Size: 1215 687
Enzymes that do cut:
AarI AccI AcuI AflII AleI AloI AlwNI ApoI
AvaI BamHI BbsI BbvCI BcgI BciVI BclI BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI
BstAPI BstYI BtgI BtsI DraI DrdI EarI Eco57MI
EcoNI EcoO109I EcoRV Hin4I HincII HindIII HpaI MmeI
MslI NcoI PasI PshAI PstI SalI SapI SfcI
SmlI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BanII BbeI BfrBI BglI BglII BmgBI BmrI BmtI
BsaAI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI
BspEI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI EagI
EciI EcoICRI EcoRI FalI FseI FspI FspAI HaeII
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI