Restriction map for
pF1KA0683
(Linear) (Six-base) MAPSORT of: KIAA0683.seq Check: 5264 from: 1 to: 2511
KIAA0683.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 990 2511
Size: 990 1521
AccI GT'mk_AC
Cuts at: 0 1625 2511
Size: 1625 886
Acc65I G'GTAC_C
Cuts at: 0 702 2511
Size: 702 1809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 1027 1537 2095 2511
Size: 124 903 510 558 416
Fragments arranged by size:
903 558 510 416 124
AfeI AGC'GCT
Cuts at: 0 137 719 1588 2116 2511
Size: 137 582 869 528 395
Fragments arranged by size:
869 582 528 395 137
AflIII A'CryG_T
Cuts at: 0 1496 2511
Size: 1496 1015
AhdI GACnn_n'nnGTC
Cuts at: 0 31 2511
Size: 31 2480
AleI CACnn'nnGTG
Cuts at: 0 2224 2511
Size: 2224 287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 526 754 2266 2511
Size: 526 228 1512 245
Fragments arranged by size:
1512 526 245 228
AlwNI CAG_nnn'CTG
Cuts at: 0 951 1005 1866 2307 2511
Size: 951 54 861 441 204
Fragments arranged by size:
951 861 441 204 54
ApaI G_GGCC'C
Cuts at: 0 283 337 1611 2053 2199 2271 2460 2511
Size: 283 54 1274 442 146 72 189 51
Fragments arranged by size:
1274 442 283 189 146 72 54 51
ApoI r'AATT_y
Cuts at: 0 1273 2257 2511
Size: 1273 984 254
Fragments arranged by size:
1273 984 254
AvaI C'yCGr_G
Cuts at: 0 141 1111 1255 1303 1646 1885 2040 2511
Size: 141 970 144 48 343 239 155 471
Fragments arranged by size:
970 471 343 239 155 144 141 48
BamHI G'GATC_C
Cuts at: 0 1247 2511
Size: 1247 1264
BanI G'GyrC_C
Cuts at: 0 702 758 983 1345 1880 2511
Size: 702 56 225 362 535 631
Fragments arranged by size:
702 631 535 362 225 56
BanII G_rGCy'C
Cuts at: 0 214 283 337 1391 1453 1611 1636 1737
Size: 214 69 54 1054 62 158 25 101
Cuts at: 1737 2053 2199 2271 2460 2511
Size: 316 146 72 189 51
Fragments arranged by size:
1054 316 214 189 158 146 101 72
69 62 54 51 25
BbeI G_GCGC'C
Cuts at: 0 762 987 1349 2511
Size: 762 225 362 1162
Fragments arranged by size:
1162 762 362 225
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 1702 2162 2511
Size: 53 1649 460 349
Fragments arranged by size:
1649 460 349 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 310 344 921 955 1350 1384 2511
Size: 310 34 577 34 395 34 1127
Fragments arranged by size:
1127 577 395 310 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 563 793 956 1354 1606 1741 1771 2057
Size: 563 230 163 398 252 135 30 286
Cuts at: 2057 2511
Size: 454
Fragments arranged by size:
563 454 398 286 252 230 163 135
30
BlpI GC'TnA_GC
Cuts at: 0 208 2335 2511
Size: 208 2127 176
Fragments arranged by size:
2127 208 176
Bme1580I G_kGCm'C
Cuts at: 0 283 337 1245 1363 1611 2053 2199 2271
Size: 283 54 908 118 248 442 146 72
Cuts at: 2271 2460 2511
Size: 189 51
Fragments arranged by size:
908 442 283 248 189 146 118 72
54 51
BmgBI CAC'GTC
Cuts at: 0 1025 1077 1364 1571 2409 2511
Size: 1025 52 287 207 838 102
Fragments arranged by size:
1025 838 287 207 102 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 835 879 2511
Size: 835 44 1632
Fragments arranged by size:
1632 835 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 180 2511
Size: 180 2331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 116 244 268 295 1708 2380 2511
Size: 115 1 128 24 27 1413 672 131
Fragments arranged by size:
1413 672 131 128 115 27 24 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 2464 2511
Size: 1633 831 47
Fragments arranged by size:
1633 831 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 1393 2511
Size: 307 1086 1118
Fragments arranged by size:
1118 1086 307
BsaAI yAC'GTr
Cuts at: 0 2295 2511
Size: 2295 216
BsaHI Gr'CG_yC
Cuts at: 0 759 934 984 1209 1346 1427 1972 2511
Size: 759 175 50 225 137 81 545 539
Fragments arranged by size:
759 545 539 225 175 137 81 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 271 403 433 2511
Size: 241 30 132 30 2078
Fragments arranged by size:
2078 241 132 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 265 583 1705 2011 2313 2482 2511
Size: 169 96 318 1122 306 302 169 29
Fragments arranged by size:
1122 318 306 302 169 169 96 29
BseYI C'CCAG_C
Cuts at: 0 213 337 468 852 954 1328 1589 2018
Size: 213 124 131 384 102 374 261 429
Cuts at: 2018 2065 2511
Size: 47 446
Fragments arranged by size:
446 429 384 374 261 213 131 124
102 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 724 2511
Size: 72 652 1787
Fragments arranged by size:
1787 652 72
BsiEI CG_ry'CG
Cuts at: 0 2318 2511
Size: 2318 193
BsmBI CGTCTCn'nnnn_
Cuts at: 0 454 1015 1953 2325 2510 2511
Size: 454 561 938 372 185 1
Fragments arranged by size:
938 561 454 372 185 1
Bsp1286I G_dGCh'C
Cuts at: 0 214 283 337 1245 1363 1391 1453 1611
Size: 214 69 54 908 118 28 62 158
Cuts at: 1611 1636 1737 2053 2199 2271 2460 2511
Size: 25 101 316 146 72 189 51
Fragments arranged by size:
908 316 214 189 158 146 118 101
72 69 62 54 51 28 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 746 990 2511
Size: 746 244 1521
Fragments arranged by size:
1521 746 244
BsrBI CCG'CTC
Cuts at: 0 990 2001 2511
Size: 990 1011 510
Fragments arranged by size:
1011 990 510
BsrFI r'CCGG_y
Cuts at: 0 392 2086 2511
Size: 392 1694 425
Fragments arranged by size:
1694 425 392
BssHII G'CGCG_C
Cuts at: 0 2350 2511
Size: 2350 161
BstAPI GCAn_nnn'nTGC
Cuts at: 0 951 2511
Size: 951 1560
BstEII G'GTnAC_C
Cuts at: 0 2133 2511
Size: 2133 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1161 2511
Size: 1161 1350
BstYI r'GATC_y
Cuts at: 0 686 1247 2511
Size: 686 561 1264
Fragments arranged by size:
1264 686 561
Bsu36I CC'TnA_GG
Cuts at: 0 833 2190 2511
Size: 833 1357 321
Fragments arranged by size:
1357 833 321
BtgI C'CryG_G
Cuts at: 0 237 669 788 968 1379 1967 2081 2222
Size: 237 432 119 180 411 588 114 141
Cuts at: 2222 2237 2511
Size: 15 274
Fragments arranged by size:
588 432 411 274 237 180 141 119
114 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1487 2511
Size: 1487 1024
BtsI GCAGTG_nn'
Cuts at: 0 407 424 1044 1968 2511
Size: 407 17 620 924 543
Fragments arranged by size:
924 620 543 407 17
ClaI AT'CG_AT
Cuts at: 0 2285 2511
Size: 2285 226
DraIII CAC_nnn'GTG
Cuts at: 0 1928 2511
Size: 1928 583
EaeI y'GGCC_r
Cuts at: 0 75 240 371 527 965 1151 1667 1742
Size: 75 165 131 156 438 186 516 75
Cuts at: 1742 1772 2084 2088 2234 2315 2511
Size: 30 312 4 146 81 196
Fragments arranged by size:
516 438 312 196 186 165 156 146
131 81 75 75 30 4
EagI C'GGCC_G
Cuts at: 0 2315 2511
Size: 2315 196
EarI CTCTTCn'nnn_
Cuts at: 0 1280 1513 2511
Size: 1280 233 998
Fragments arranged by size:
1280 998 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 301 544 1036 2419 2511
Size: 301 243 492 1383 92
Fragments arranged by size:
1383 492 301 243 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 116 124 244 268 295 1027 1537
Size: 115 1 8 120 24 27 732 510
Cuts at: 1537 1708 2095 2380 2511
Size: 171 387 285 131
Fragments arranged by size:
732 510 387 285 171 131 120 115
27 24 8 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 385 514 997 1936 2359 2511
Size: 385 129 483 939 423 152
Fragments arranged by size:
939 483 423 385 152 129
EcoO109I rG'GnC_Cy
Cuts at: 0 279 334 648 829 837 1261 1608 1617
Size: 279 55 314 181 8 424 347 9
Cuts at: 1617 2049 2195 2196 2247 2268 2364 2456 2457
Size: 432 146 1 51 21 96 92 1
Cuts at: 2457 2511
Size: 54
Fragments arranged by size:
432 424 347 314 279 181 146 96
92 55 54 51 21 9 8 1
1
EcoRI G'AATT_C
Cuts at: 0 2257 2511
Size: 2257 254
FseI GG_CCGG'CC
Cuts at: 0 2090 2511
Size: 2090 421
HaeII r_GCGC'y
Cuts at: 0 139 721 762 987 1074 1349 1590 2118
Size: 139 582 41 225 87 275 241 528
Cuts at: 2118 2511
Size: 393
Fragments arranged by size:
582 528 393 275 241 225 139 87
41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 114 146 1333 1365 2511
Size: 17 32 65 32 1187 32 1146
Fragments arranged by size:
1187 1146 65 32 32 32 17
HincII GTy'rAC
Cuts at: 0 2217 2511
Size: 2217 294
HindIII A'AGCT_T
Cuts at: 0 904 2511
Size: 904 1607
HpaI GTT'AAC
Cuts at: 0 2217 2511
Size: 2217 294
KasI G'GCGC_C
Cuts at: 0 758 983 1345 2511
Size: 758 225 362 1166
Fragments arranged by size:
1166 758 362 225
KpnI G_GTAC'C
Cuts at: 0 706 2511
Size: 706 1805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 2511
Size: 998 1513
MscI TGG'CCA
Cuts at: 0 373 967 1153 1669 2236 2511
Size: 373 594 186 516 567 275
Fragments arranged by size:
594 567 516 373 275 186
MslI CAynn'nnrTG
Cuts at: 0 1222 2224 2285 2511
Size: 1222 1002 61 226
Fragments arranged by size:
1222 1002 226 61
MspA1I CmG'CkG
Cuts at: 0 981 1443 2022 2163 2511
Size: 981 462 579 141 348
Fragments arranged by size:
981 579 462 348 141
NaeI GCC'GGC
Cuts at: 0 394 2088 2511
Size: 394 1694 423
Fragments arranged by size:
1694 423 394
NarI GG'CG_CC
Cuts at: 0 759 984 1346 2511
Size: 759 225 362 1165
Fragments arranged by size:
1165 759 362 225
NcoI C'CATG_G
Cuts at: 0 237 788 968 2237 2511
Size: 237 551 180 1269 274
Fragments arranged by size:
1269 551 274 237 180
NgoMIV G'CCGG_C
Cuts at: 0 392 2086 2511
Size: 392 1694 425
Fragments arranged by size:
1694 425 392
NspI r_CATG'y
Cuts at: 0 1500 2511
Size: 1500 1011
PasI CC'CwG_GG
Cuts at: 0 726 2511
Size: 726 1785
PciI A'CATG_T
Cuts at: 0 1496 2511
Size: 1496 1015
PfoI T'CCnGG_A
Cuts at: 0 35 272 455 1544 1835 2511
Size: 35 237 183 1089 291 676
Fragments arranged by size:
1089 676 291 237 183 35
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1286 1318 2511
Size: 1286 32 1193
Fragments arranged by size:
1286 1193 32
PpuMI rG'GwC_Cy
Cuts at: 0 648 837 2364 2511
Size: 648 189 1527 147
Fragments arranged by size:
1527 648 189 147
PspOMI G'GGCC_C
Cuts at: 0 279 333 1607 2049 2195 2267 2456 2511
Size: 279 54 1274 442 146 72 189 55
Fragments arranged by size:
1274 442 279 189 146 72 55 54
PspXI vC'TCGA_Gb
Cuts at: 0 1303 2511
Size: 1303 1208
PstI C_TGCA'G
Cuts at: 0 515 590 947 1001 1398 2440 2474 2511
Size: 515 75 357 54 397 1042 34 37
Fragments arranged by size:
1042 515 397 357 75 54 37 34
PvuII CAG'CTG
Cuts at: 0 1443 2163 2511
Size: 1443 720 348
Fragments arranged by size:
1443 720 348
RsrII CG'GwC_CG
Cuts at: 0 780 2511
Size: 780 1731
SapI GCTCTTCn'nnn_
Cuts at: 0 1513 2511
Size: 1513 998
SexAI A'CCwGG_T
Cuts at: 0 860 2511
Size: 860 1651
SfcI C'TryA_G
Cuts at: 0 511 586 943 997 1394 1626 1809 2436
Size: 511 75 357 54 397 232 183 627
Cuts at: 2436 2470 2511
Size: 34 41
Fragments arranged by size:
627 511 397 357 232 183 75 54
41 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1771 2511
Size: 1771 740
SfoI GGC'GCC
Cuts at: 0 760 985 1347 2511
Size: 760 225 362 1164
Fragments arranged by size:
1164 760 362 225
SmlI C'TyrA_G
Cuts at: 0 1303 1612 2479 2511
Size: 1303 309 867 32
Fragments arranged by size:
1303 867 309 32
StuI AGG'CCT
Cuts at: 0 1875 2127 2511
Size: 1875 252 384
Fragments arranged by size:
1875 384 252
StyI C'CwwG_G
Cuts at: 0 237 609 651 788 968 1316 1670 2030
Size: 237 372 42 137 180 348 354 360
Cuts at: 2030 2237 2511
Size: 207 274
Fragments arranged by size:
372 360 354 348 274 237 207 180
137 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 739 1868 2511
Size: 739 1129 643
Fragments arranged by size:
1129 739 643
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 241 273 403 435 648 680 2248 2280
Size: 241 32 130 32 213 32 1568 32
Cuts at: 2280 2511
Size: 231
Fragments arranged by size:
1568 241 231 213 130 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1569 2511
Size: 1569 942
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 270 2229 2511
Size: 270 1959 282
Fragments arranged by size:
1959 282 270
XhoI C'TCGA_G
Cuts at: 0 1303 2511
Size: 1303 1208
XmnI GAAnn'nnTTC
Cuts at: 0 44 550 2511
Size: 44 506 1961
Fragments arranged by size:
1961 506 44
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PpiI
PpuMI PspOMI PspXI PstI PvuII RsrII SapI SexAI
SfcI SfiI SfoI SmlI StuI StyI TaqII TstI
Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglII
BmtI Bpu10I BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstZ17I CspCI DraI
DrdI EcoICRI EcoRV FalI FspI FspAI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PflMI PmeI
PmlI PshAI PsiI PsrI PvuI SacI SacII SalI
SanDI SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XmaI ZraI