Restriction map for
pF1KSDA0693
(Linear) (Six-base) MAPSORT of: KIAA0693.seq Check: 1785 from: 1 to: 1188
KIAA0693.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 176 1188
Size: 176 1012
Acc65I G'GTAC_C
Cuts at: 0 947 1188
Size: 947 241
AflIII A'CryG_T
Cuts at: 0 386 518 1188
Size: 386 132 670
Fragments arranged by size:
670 386 132
AgeI A'CCGG_T
Cuts at: 0 875 1188
Size: 875 313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 1188
Size: 352 836
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1188
Size: 199 32 957
Fragments arranged by size:
957 199 32
AlwNI CAG_nnn'CTG
Cuts at: 0 159 219 294 738 1188
Size: 159 60 75 444 450
Fragments arranged by size:
450 444 159 75 60
ApaI G_GGCC'C
Cuts at: 0 259 449 1188
Size: 259 190 739
Fragments arranged by size:
739 259 190
ApaLI G'TGCA_C
Cuts at: 0 138 1188
Size: 138 1050
AvaI C'yCGr_G
Cuts at: 0 1015 1188
Size: 1015 173
BanI G'GyrC_C
Cuts at: 0 405 502 696 784 947 958 1188
Size: 405 97 194 88 163 11 230
Fragments arranged by size:
405 230 194 163 97 88 11
BanII G_rGCy'C
Cuts at: 0 259 267 449 797 1053 1188
Size: 259 8 182 348 256 135
Fragments arranged by size:
348 259 256 182 135 8
BbeI G_GCGC'C
Cuts at: 0 409 700 788 1188
Size: 409 291 88 400
Fragments arranged by size:
409 400 291 88
BglI GCCn_nnn'nGGC
Cuts at: 0 368 705 1188
Size: 368 337 483
Fragments arranged by size:
483 368 337
Bme1580I G_kGCm'C
Cuts at: 0 142 259 449 1188
Size: 142 117 190 739
Fragments arranged by size:
739 190 142 117
BmgBI CAC'GTC
Cuts at: 0 584 1188
Size: 584 604
BmrI ACTGGGnnnn_n'
Cuts at: 0 547 918 1188
Size: 547 371 270
Fragments arranged by size:
547 371 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 267 281 1188
Size: 127 140 14 907
Fragments arranged by size:
907 140 127 14
BsaAI yAC'GTr
Cuts at: 0 387 519 1188
Size: 387 132 669
Fragments arranged by size:
669 387 132
BsaHI Gr'CG_yC
Cuts at: 0 320 406 482 697 785 1188
Size: 320 86 76 215 88 403
Fragments arranged by size:
403 320 215 88 86 76
BsaWI w'CCGG_w
Cuts at: 0 164 875 1188
Size: 164 711 313
Fragments arranged by size:
711 313 164
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 694 724 1188
Size: 541 30 123 30 464
Fragments arranged by size:
541 464 123 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 336 763 901 1188
Size: 336 427 138 287
Fragments arranged by size:
427 336 287 138
BseYI C'CCAG_C
Cuts at: 0 713 728 983 997 1133 1188
Size: 713 15 255 14 136 55
Fragments arranged by size:
713 255 136 55 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 280 1188
Size: 145 135 908
Fragments arranged by size:
908 145 135
BsiEI CG_ry'CG
Cuts at: 0 134 191 876 1188
Size: 134 57 685 312
Fragments arranged by size:
685 312 134 57
BsiHKAI G_wGCw'C
Cuts at: 0 142 1113 1188
Size: 142 971 75
Fragments arranged by size:
971 142 75
Bsp1286I G_dGCh'C
Cuts at: 0 142 259 267 449 797 1053 1113 1188
Size: 142 117 8 182 348 256 60 75
Fragments arranged by size:
348 256 182 142 117 75 60 8
BsrBI CCG'CTC
Cuts at: 0 278 1188
Size: 278 910
BsrFI r'CCGG_y
Cuts at: 0 704 875 1188
Size: 704 171 313
Fragments arranged by size:
704 313 171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 159 219 1188
Size: 159 60 969
Fragments arranged by size:
969 159 60
BstYI r'GATC_y
Cuts at: 0 155 1188
Size: 155 1033
BstZ17I GTA'TAC
Cuts at: 0 177 1188
Size: 177 1011
BtgI C'CryG_G
Cuts at: 0 5 692 788 797 825 1188
Size: 5 687 96 9 28 363
Fragments arranged by size:
687 363 96 28 9 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 624 1188
Size: 624 564
DrdI GACnn_nn'nnGTC
Cuts at: 0 1125 1188
Size: 1125 63
EaeI y'GGCC_r
Cuts at: 0 25 131 182 578 822 1188
Size: 25 106 51 396 244 366
Fragments arranged by size:
396 366 244 106 51 25
EagI C'GGCC_G
Cuts at: 0 131 1188
Size: 131 1057
EarI CTCTTCn'nnn_
Cuts at: 0 729 1188
Size: 729 459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 267 281 1188
Size: 127 140 14 907
Fragments arranged by size:
907 140 127 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 742 1188
Size: 355 387 446
Fragments arranged by size:
446 387 355
EcoO109I rG'GnC_Cy
Cuts at: 0 256 291 466 1188
Size: 256 35 175 722
Fragments arranged by size:
722 256 175 35
HaeII r_GCGC'y
Cuts at: 0 409 700 788 1126 1188
Size: 409 291 88 338 62
Fragments arranged by size:
409 338 291 88 62
KasI G'GCGC_C
Cuts at: 0 405 696 784 1188
Size: 405 291 88 404
Fragments arranged by size:
405 404 291 88
KpnI G_GTAC'C
Cuts at: 0 951 1188
Size: 951 237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 572 1188
Size: 224 348 616
Fragments arranged by size:
616 348 224
MscI TGG'CCA
Cuts at: 0 184 1188
Size: 184 1004
MslI CAynn'nnrTG
Cuts at: 0 430 538 643 905 1188
Size: 430 108 105 262 283
Fragments arranged by size:
430 283 262 108 105
MspA1I CmG'CkG
Cuts at: 0 687 790 1143 1188
Size: 687 103 353 45
Fragments arranged by size:
687 353 103 45
NarI GG'CG_CC
Cuts at: 0 406 697 785 1188
Size: 406 291 88 403
Fragments arranged by size:
406 403 291 88
NcoI C'CATG_G
Cuts at: 0 692 797 825 1188
Size: 692 105 28 363
Fragments arranged by size:
692 363 105 28
NspI r_CATG'y
Cuts at: 0 213 543 1188
Size: 213 330 645
Fragments arranged by size:
645 330 213
PasI CC'CwG_GG
Cuts at: 0 1044 1188
Size: 1044 144
PfoI T'CCnGG_A
Cuts at: 0 104 1188
Size: 104 1084
PmlI CAC'GTG
Cuts at: 0 387 1188
Size: 387 801
PpuMI rG'GwC_Cy
Cuts at: 0 466 1188
Size: 466 722
PspOMI G'GGCC_C
Cuts at: 0 255 445 1188
Size: 255 190 743
Fragments arranged by size:
743 255 190
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 160 192 1188
Size: 160 32 996
Fragments arranged by size:
996 160 32
PstI C_TGCA'G
Cuts at: 0 359 1188
Size: 359 829
PvuI CG_AT'CG
Cuts at: 0 191 1188
Size: 191 997
SacII CC_GC'GG
Cuts at: 0 791 1188
Size: 791 397
SanDI GG'GwC_CC
Cuts at: 0 466 1188
Size: 466 722
SapI GCTCTTCn'nnn_
Cuts at: 0 729 1188
Size: 729 459
SbfI CC_TGCA'GG
Cuts at: 0 359 1188
Size: 359 829
ScaI AGT'ACT
Cuts at: 0 754 811 1188
Size: 754 57 377
Fragments arranged by size:
754 377 57
SexAI A'CCwGG_T
Cuts at: 0 170 1188
Size: 170 1018
SfcI C'TryA_G
Cuts at: 0 355 453 591 1188
Size: 355 98 138 597
Fragments arranged by size:
597 355 138 98
SfoI GGC'GCC
Cuts at: 0 407 698 786 1188
Size: 407 291 88 402
Fragments arranged by size:
407 402 291 88
SmaI CCC'GGG
Cuts at: 0 1017 1188
Size: 1017 171
StyI C'CwwG_G
Cuts at: 0 692 797 825 1083 1188
Size: 692 105 28 258 105
Fragments arranged by size:
692 258 105 105 28
TatI w'GTAC_w
Cuts at: 0 752 767 809 926 1188
Size: 752 15 42 117 262
Fragments arranged by size:
752 262 117 42 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 645 1188
Size: 645 543
XmaI C'CCGG_G
Cuts at: 0 1015 1188
Size: 1015 173
Enzymes that do cut:
AccI Acc65I AflIII AgeI AlfI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BglI Bme1580I BmgBI
BmrI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BstAPI BstYI
BstZ17I BtgI BtgZI DrdI EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII KasI KpnI MmeI MscI MslI
MspA1I NarI NcoI NspI PasI PfoI PmlI PpuMI
PspOMI PsrI PstI PvuI SacII SanDI SapI SbfI
ScaI SexAI SfcI SfoI SmaI StyI TatI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AhdI AleI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI Bsu36I BtsI ClaI CspCI DraI DraIII
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PvuII RsrII SacI
SalI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmnI ZraI